Planarian EST Database


Dr_sW_003_L03

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_003_L03
         (828 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9QZA6|CD151_RAT  Platelet endothelial tetraspan antigen ...   115   1e-25
sp|O35566|C151_MOUSE  Platelet-endothelial tetraspan antigen...   113   7e-25
sp|P48509|CD151_HUMAN  Platelet-endothelial tetraspan antige...   109   1e-23
sp|P28648|CD63_RAT  CD63 antigen (AD1 antigen)                    108   2e-23
sp|Q9XSK2|CD63_BOVIN  CD63 antigen                                107   3e-23
sp|P61171|CD151_MACMU  Platelet-endothelial tetraspan antige...   104   3e-22
sp|O60637|TSN3_HUMAN  Tetraspanin-3 (Tspan-3) (Transmembrane...   103   6e-22
sp|Q9QY33|TSN3_MOUSE  Tetraspanin-3 (Tspan-3) (Transmembrane...   103   6e-22
sp|P08962|CD63_HUMAN  CD63 antigen (Melanoma-associated anti...   102   1e-21
sp|P41731|CD63_MOUSE  CD63 antigen                                100   6e-21
>sp|Q9QZA6|CD151_RAT Platelet endothelial tetraspan antigen 3 (CD151 antigen)
          Length = 253

 Score =  115 bits (289), Expect = 1e-25
 Identities = 62/222 (27%), Positives = 110/222 (49%), Gaps = 4/222 (1%)
 Frame = +2

Query: 56  WLSGLALIVISAFFLANGTMKYVDEIINSSTNKGLLYGILAVGVIIFVIAFCGCCGAIKD 235
           WL+GLA++ +  + LA  +  Y+  ++ SST     Y ++  GV++ V    GCC   K+
Sbjct: 28  WLAGLAVMAVGIWTLALKS-DYIS-LLASSTYLATAYILVVAGVVVMVTGVLGCCATFKE 85

Query: 236 SKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQNEFSKAFQKIY--GNDTTVTKT 409
            + LL +Y  LL ++ + E + GI A V    +   ++        K Y       VT  
Sbjct: 86  RRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKDTMIKRYHQSGHEGVTNA 145

Query: 410 VDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPSSCCDPITSPCWELGYDKKAF--T 583
           VD LQQ   CCG+    DW  S+W ++ +  ++  P SCC  + + C +  +    +   
Sbjct: 146 VDKLQQEFHCCGSNNSRDWRDSEWIRSGEADSRVVPDSCCKTVVTGCGKREHASNIYKVE 205

Query: 584 DGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCLCRN 709
            GCI K+ S+ + +  ++  + +GIA  ++F ++   CL R+
Sbjct: 206 GGCITKLESFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRS 247
>sp|O35566|C151_MOUSE Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
           glycoprotein SFA-1) (CD151 antigen)
          Length = 253

 Score =  113 bits (282), Expect = 7e-25
 Identities = 60/222 (27%), Positives = 110/222 (49%), Gaps = 4/222 (1%)
 Frame = +2

Query: 56  WLSGLALIVISAFFLANGTMKYVDEIINSSTNKGLLYGILAVGVIIFVIAFCGCCGAIKD 235
           WL+GLA++ +  + LA  +  Y+  ++ SST     Y ++  GV++ V    GCC   K+
Sbjct: 28  WLAGLAVMAVGIWTLALKS-DYIS-LLASSTYLATAYILVVAGVVVMVTGVLGCCATFKE 85

Query: 236 SKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQNEFSKAFQKIY--GNDTTVTKT 409
            + LL +Y  LL ++ + E + GI A V    +   ++        K Y       V+  
Sbjct: 86  RRNLLRLYFILLLIIFLLEIIAGILAYVYYQQLNTELKENLKDTMVKRYHQSGHEGVSSA 145

Query: 410 VDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPSSCCDPITSPCWELGYDKKAF--T 583
           VD LQQ   CCG+    DW  S+W ++ +  ++  P SCC  + + C +  +    +   
Sbjct: 146 VDKLQQEFHCCGSNNSQDWQDSEWIRSGEADSRVVPDSCCKTMVAGCGKRDHASNIYKVE 205

Query: 584 DGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCLCRN 709
            GCI K+ ++ + +  ++  + +GIA  ++F ++   CL R+
Sbjct: 206 GGCITKLETFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRS 247
>sp|P48509|CD151_HUMAN Platelet-endothelial tetraspan antigen 3 (PETA-3) (GP27) (Membrane
           glycoprotein SFA-1) (CD151 antigen) (Tetraspanin-24)
           (Tspan-24)
          Length = 253

 Score =  109 bits (272), Expect = 1e-23
 Identities = 57/222 (25%), Positives = 106/222 (47%), Gaps = 4/222 (1%)
 Frame = +2

Query: 56  WLSGLALIVISAFFLANGTMKYVDEIINSSTNKGLLYGILAVGVIIFVIAFCGCCGAIKD 235
           WL+GLA++ +  + LA  +  Y+  ++ S T     Y ++  G ++ V    GCC   K+
Sbjct: 28  WLAGLAVMAVGIWTLALKS-DYIS-LLASGTYLATAYILVVAGTVVMVTGVLGCCATFKE 85

Query: 236 SKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQNEFSKAFQKIY--GNDTTVTKT 409
            + LL +Y  LL ++ + E + GI A      +   ++        + Y       VT  
Sbjct: 86  RRNLLRLYFILLLIIFLLEIIAGILAYAYYQQLNTELKENLKDTMTRRYHQSGHEAVTSA 145

Query: 410 VDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPSSCCDPITSPCWELGYDKKAF--T 583
           VD LQQ   CCG+    DW  S+W ++ +   +  P SCC  + + C +  +    +   
Sbjct: 146 VDQLQQEFHCCGSNNSQDWRDSEWIRSQEAGGRVVPDSCCKTVVALCGQRDHASNIYKVE 205

Query: 584 DGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCLCRN 709
            GCI K+ ++ + +  ++  + +GIA  ++F ++   CL R+
Sbjct: 206 GGCITKLETFIQEHLRVIGAVGIGIACVQVFGMIFTCCLYRS 247
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
          Length = 238

 Score =  108 bits (270), Expect = 2e-23
 Identities = 62/183 (33%), Positives = 96/183 (52%), Gaps = 3/183 (1%)
 Frame = +2

Query: 170 ILAVGVIIFVIAFCGCCGAIKDSKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQ 349
           I+AVG  +F++AF GCCGA K++  L+  +   L L+++ E    I   V R    + ++
Sbjct: 57  IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFR----DQVK 112

Query: 350 NEFSKAFQKIYGNDTTVTKT---VDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPS 520
           +EFSK+FQK   N  T  KT   +D LQ+  +CCGA+  TDW +       +      P 
Sbjct: 113 SEFSKSFQKQMQNYLTDNKTATILDKLQKENKCCGASNYTDWERIPGMAKDR-----VPD 167

Query: 521 SCCDPITSPCWELGYDKKAFTDGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCL 700
           SCC  IT  C     +    T GC+  + ++ +    LV G  LGIAF E+  ++ + CL
Sbjct: 168 SCCINITVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAFVEVLGIIFSCCL 227

Query: 701 CRN 709
            ++
Sbjct: 228 VKS 230
>sp|Q9XSK2|CD63_BOVIN CD63 antigen
          Length = 237

 Score =  107 bits (268), Expect = 3e-23
 Identities = 61/183 (33%), Positives = 94/183 (51%), Gaps = 3/183 (1%)
 Frame = +2

Query: 170 ILAVGVIIFVIAFCGCCGAIKDSKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQ 349
           I+AVG  +F++AF GCCGA K++  L+  +   L L+++ E    I   V R    + ++
Sbjct: 57  IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFR----DKVR 112

Query: 350 NEFSKAFQ---KIYGNDTTVTKTVDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPS 520
           +EF+K F+   K Y  D      +D +Q+  +CCGA   TDW      +     T   P 
Sbjct: 113 SEFNKDFRQQMKNYPKDNQTASILDKMQKDFECCGAANYTDW------EKILAVTNKVPD 166

Query: 521 SCCDPITSPCWELGYDKKAFTDGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCL 700
           SCC  IT  C      K   T+GC+ K+ ++ +    +V+   LGIAF EI  +V A CL
Sbjct: 167 SCCVNITHNCGINFVVKDIHTEGCVEKIAAWLRKNVLVVVAAALGIAFVEILGIVLACCL 226

Query: 701 CRN 709
            ++
Sbjct: 227 VKS 229
>sp|P61171|CD151_MACMU Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
 sp|P61170|CD151_CERAE Platelet-endothelial tetraspan antigen 3 (PETA-3) (CD151 antigen)
          Length = 253

 Score =  104 bits (259), Expect = 3e-22
 Identities = 57/222 (25%), Positives = 105/222 (47%), Gaps = 4/222 (1%)
 Frame = +2

Query: 56  WLSGLALIVISAFFLANGTMKYVDEIINSSTNKGLLYGILAVGVIIFVIAFCGCCGAIKD 235
           WL+GLA++ +  + LA  +  Y+  ++ S T     Y ++  G ++ V    GCC   K+
Sbjct: 28  WLAGLAVMAVGIWTLALKS-DYIS-LLASGTYLATAYILVVAGAVVMVTGVLGCCATFKE 85

Query: 236 SKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQNEFSKAFQKIYGND--TTVTKT 409
            + LL +Y  LL ++ + E + G+ A V    +   ++        K Y       VT  
Sbjct: 86  RRNLLRLYFILLLIIFLLEIIAGVLAYVYYQQLNTELKENLKDTMAKRYHQPGHEAVTSA 145

Query: 410 VDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPSSCCDPITSPCWELGYDKKAF--T 583
           VD LQQ   CCG+    DW  S+W +  + + +  P SCC  + + C +  +    +   
Sbjct: 146 VDQLQQEFHCCGSNNSQDWRDSEWIRLREARGRVVPDSCCKTVVAGCGQRDHAFNIYKVE 205

Query: 584 DGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCLCRN 709
            G I K+ ++ + +  ++  +  GIA  ++F ++   CL R+
Sbjct: 206 GGFITKLETFIQEHLRVIGAVGTGIACVQVFGMIFTCCLYRS 247
>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
           (Tetraspanin TM4-A)
          Length = 253

 Score =  103 bits (257), Expect = 6e-22
 Identities = 57/188 (30%), Positives = 93/188 (49%), Gaps = 7/188 (3%)
 Frame = +2

Query: 170 ILAVGVIIFVIAFCGCCGAIKDSKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQ 349
           I+AVG ++F+I   GCC  I++S+  L  ++ +L LV + E V  +   V R  VE    
Sbjct: 57  IIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEVVVVVLGYVYRAKVE---- 112

Query: 350 NEFSKAFQKIY-----GNDTTVTKTVDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPF 514
           NE  ++ QK+Y      N    ++ +D +Q+ + CCG    +DW  +DW++ +KN  Q  
Sbjct: 113 NEVDRSIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKN--QSV 170

Query: 515 PSSCCDPITSPC-WELGYDKKAFTDGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCA 691
           P SCC    S C   L +    + +GC   +    +     VI   L  A  ++  ++CA
Sbjct: 171 PLSCCRETASNCNGSLAHPSDLYAEGCEALVVKKLQEIMMHVIWAALAFAAIQLLGMLCA 230

Query: 692 -FCLCRNT 712
              LCR +
Sbjct: 231 CIVLCRRS 238
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
           (Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
          Length = 253

 Score =  103 bits (257), Expect = 6e-22
 Identities = 57/188 (30%), Positives = 92/188 (48%), Gaps = 7/188 (3%)
 Frame = +2

Query: 170 ILAVGVIIFVIAFCGCCGAIKDSKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQ 349
           I+AVG ++F+I   GCC  I++S+  L  ++F+L LV + E V  +   V R  VE    
Sbjct: 57  IIAVGALLFIIGLIGCCATIRESRCGLATFVFILLLVFVTEVVVVVLGYVYRAKVE---- 112

Query: 350 NEFSKAFQKIY-----GNDTTVTKTVDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPF 514
           NE  ++ QK+Y      N    ++ +D +Q+ + CCG    +DW  +DW++ +KN  Q  
Sbjct: 113 NEVDRSIQKVYKTYNGTNSDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKETKN--QSV 170

Query: 515 PSSCCDPITSPC-WELGYDKKAFTDGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCA 691
           P SCC      C   L      + +GC   +    +     VI   L  A  ++  ++CA
Sbjct: 171 PLSCCRETAKSCNGSLANPSDLYAEGCEALVVKKLQEILMHVIWAALAFAAIQLLGMLCA 230

Query: 692 -FCLCRNT 712
              LCR +
Sbjct: 231 CIVLCRRS 238
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
           (Lysosome-associated membrane glycoprotein 3) (LAMP-3)
           (Ocular melanoma-associated antigen) (OMA81H)
           (Granulophysin) (Tetraspanin-30) (Tspan-30)
          Length = 238

 Score =  102 bits (254), Expect = 1e-21
 Identities = 64/194 (32%), Positives = 97/194 (50%), Gaps = 1/194 (0%)
 Frame = +2

Query: 131 IINSSTNKGLL-YGILAVGVIIFVIAFCGCCGAIKDSKMLLGIYIFLLFLVIIAEAVFGI 307
           II  +T   LL   I+AVGV +F++AF GCCGA K++  L+  +   L L+++ E    I
Sbjct: 43  IIQGATPGSLLPVVIIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAI 102

Query: 308 YAIVKRPAVEEMIQNEFSKAFQKIYGNDTTVTKTVDSLQQAMQCCGATKMTDWLQSDWYQ 487
              V R  V     N F +  +    N+ T +  +D +Q   +CCGA   TDW +     
Sbjct: 103 AGYVFRDKVMSEFNNNFRQQMENYPKNNHTAS-ILDRMQADFKCCGAANYTDWEKIP--S 159

Query: 488 NSKNKTQPFPSSCCDPITSPCWELGYDKKAFTDGCIPKMFSYAKTYFPLVIGITLGIAFF 667
            SKN+    P SCC  +T  C     +K    +GC+ K+  + +    +V    LGIAF 
Sbjct: 160 MSKNRV---PDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFV 216

Query: 668 EIFVVVCAFCLCRN 709
           E+  +V A CL ++
Sbjct: 217 EVLGIVFACCLVKS 230
>sp|P41731|CD63_MOUSE CD63 antigen
          Length = 238

 Score =  100 bits (248), Expect = 6e-21
 Identities = 57/183 (31%), Positives = 90/183 (49%), Gaps = 3/183 (1%)
 Frame = +2

Query: 170 ILAVGVIIFVIAFCGCCGAIKDSKMLLGIYIFLLFLVIIAEAVFGIYAIVKRPAVEEMIQ 349
           I+AVG  +F++AF GCCGA K++  L+  +   L L+++ E    I   V R    + ++
Sbjct: 57  IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFR----DQVK 112

Query: 350 NEFSKAFQ---KIYGNDTTVTKTVDSLQQAMQCCGATKMTDWLQSDWYQNSKNKTQPFPS 520
           +EF+K+FQ   + Y  D      +D LQ+   CCGA+  TDW         +      P 
Sbjct: 113 SEFNKSFQQQMQNYLKDNKTATILDKLQKENNCCGASNYTDWENIPGMAKDR-----VPD 167

Query: 521 SCCDPITSPCWELGYDKKAFTDGCIPKMFSYAKTYFPLVIGITLGIAFFEIFVVVCAFCL 700
           SCC  IT  C     +    T GC+  +  + +    LV    LGIAF E+  ++ + CL
Sbjct: 168 SCCINITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGIIFSCCL 227

Query: 701 CRN 709
            ++
Sbjct: 228 VKS 230
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,033,998
Number of Sequences: 369166
Number of extensions: 1881124
Number of successful extensions: 5366
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5278
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8004331590
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)