Planarian EST Database


Dr_sW_003_J06

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_003_J06
         (557 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q86V88|MGDP1_HUMAN  Magnesium-dependent phosphatase 1 (MD...   144   2e-34
sp|Q9D967|MGDP1_MOUSE  Magnesium-dependent phosphatase 1 (MD...   138   8e-33
sp|P40081|YEW4_YEAST  Hypothetical 20.4 kDa protein in GLC7-...    58   1e-08
sp|Q9PPP0|SYE_UREPA  Glutamyl-tRNA synthetase (Glutamate--tR...    31   1.8  
sp|Q60343|Y026_METJA  Hypothetical protein MJ0026                  30   3.1  
sp|P95493|CPG2_PORGI  Gingipain R2 precursor (Gingipain 2) (...    30   3.1  
sp|Q6QEJ7|PROF_APIME  Profilin                                     30   4.0  
sp|P75114|SYE_MYCPN  Glutamyl-tRNA synthetase (Glutamate--tR...    29   6.9  
sp|P26168|BCHE_RHOCA  Anaerobic magnesium-protoporphyrin IX ...    29   9.0  
sp|P47700|SYE_MYCGE  Glutamyl-tRNA synthetase (Glutamate--tR...    29   9.0  
>sp|Q86V88|MGDP1_HUMAN Magnesium-dependent phosphatase 1 (MDP-1)
          Length = 176

 Score =  144 bits (362), Expect = 2e-34
 Identities = 71/154 (46%), Positives = 104/154 (67%), Gaps = 1/154 (0%)
 Frame = +3

Query: 24  LIVFDLDYTLWKFWIDTHVTPPFSKKNEIIV-DSYGSTVNFYPDSYFILKTLSSHPKIEV 200
           L VFDLDYTLW FW+DTHV PPF K ++  V D  G  V  YP+   +LK L S   +  
Sbjct: 7   LAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQDVRLYPEVPEVLKRLQSLG-VPG 65

Query: 201 AVASRTTEIEGAFNLIKVSGWDQFIKYKEIYPGSKMTHMAKLSRSSGVMFNRIIFFDDEE 380
           A ASRT+EIEGA  L+++    ++  ++EIYPGSK+TH  +L + +G+ F+++IFFDDE 
Sbjct: 66  AAASRTSEIEGANQLLELFDLFRYFVHREIYPGSKITHFERLQQKTGIPFSQMIFFDDER 125

Query: 381 RNVKDVSKMGVHCVLVNSGVSMKLVKDHLQDILK 482
           RN+ DVSK+GV C+ + +G++++ +   L+   K
Sbjct: 126 RNIVDVSKLGVTCIHIQNGMNLQTLSQGLETFAK 159
>sp|Q9D967|MGDP1_MOUSE Magnesium-dependent phosphatase 1 (MDP-1)
          Length = 164

 Score =  138 bits (348), Expect = 8e-33
 Identities = 68/154 (44%), Positives = 100/154 (64%), Gaps = 1/154 (0%)
 Frame = +3

Query: 24  LIVFDLDYTLWKFWIDTHVTPPFSKKNEIIV-DSYGSTVNFYPDSYFILKTLSSHPKIEV 200
           L VFDLDYTLW FW+DTHV PPF K ++  V D  G  +  YP+   +L  L S   + V
Sbjct: 7   LAVFDLDYTLWPFWVDTHVDPPFHKSSDGTVRDRRGQNIQLYPEVPEVLGRLQSLG-VPV 65

Query: 201 AVASRTTEIEGAFNLIKVSGWDQFIKYKEIYPGSKMTHMAKLSRSSGVMFNRIIFFDDEE 380
           A ASRT+EI+GA  L+++    ++   +EIYPGSK+TH  +L   +GV F++++FFDDE 
Sbjct: 66  AAASRTSEIQGANQLLELFDLGKYFIQREIYPGSKVTHFERLHHKTGVPFSQMVFFDDEN 125

Query: 381 RNVKDVSKMGVHCVLVNSGVSMKLVKDHLQDILK 482
           RN+ DV ++GV C+ +  G+S++ +   L+   K
Sbjct: 126 RNIIDVGRLGVTCIHIRDGMSLQTLTQGLETFAK 159
>sp|P40081|YEW4_YEAST Hypothetical 20.4 kDa protein in GLC7-GDI1 intergenic region
          Length = 178

 Score = 58.2 bits (139), Expect = 1e-08
 Identities = 53/167 (31%), Positives = 78/167 (46%), Gaps = 22/167 (13%)
 Frame = +3

Query: 33  FDLDYTLWKFWIDTHVTPPF----SKKNEI--IVDSYGSTVNFYPDSYFILKTLSSHPKI 194
           FDLDYT+W  + DTH+  PF    S   E+  I+   G  +  Y D   IL  L  +  +
Sbjct: 10  FDLDYTIWPCYCDTHLHGPFKPVKSSNGEVLTIICRDGYELTIYKDIPRILGDLKDN-GV 68

Query: 195 EVAVASRTTEIEGAFNLIKV-------------SGWDQFIKYKEIYPGSKMTHMAKLSRS 335
           ++  ASRT   E A  ++K+             + +D+F   +    G     +  L  +
Sbjct: 69  KLMTASRTWAPEIAQEILKIFKVKYAGVVTPLANLFDEFQWGERSKIGHLRDGLKDLYNT 128

Query: 336 SGVMFNRIIFFDDEERNVKDVSKMGVHCVLV---NSGVSMKLVKDHL 467
           S +   +I  FDDE RN K+V K GV  V V    +G S KL +D+L
Sbjct: 129 SDLKSKKICLFDDESRN-KEVEKYGVKFVYVRDPENGPSWKLYQDYL 174
>sp|Q9PPP0|SYE_UREPA Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
          Length = 482

 Score = 31.2 bits (69), Expect = 1.8
 Identities = 16/52 (30%), Positives = 23/52 (44%)
 Frame = +3

Query: 261 WDQFIKYKEIYPGSKMTHMAKLSRSSGVMFNRIIFFDDEERNVKDVSKMGVH 416
           WD  I+ K   PGS +T    L  +   M+N  +  DD E  +  V +   H
Sbjct: 165 WDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEH 216
>sp|Q60343|Y026_METJA Hypothetical protein MJ0026
          Length = 274

 Score = 30.4 bits (67), Expect = 3.1
 Identities = 20/69 (28%), Positives = 37/69 (53%), Gaps = 1/69 (1%)
 Frame = +3

Query: 264 DQFIKYKEIYPGSKMTHMAKLSRSSGVMFNRIIFFDDEERNVKDVSKMGV-HCVLVNSGV 440
           D FI      PG K TH+A+L ++ G +    I     +    ++++MGV + +++N+  
Sbjct: 84  DDFILDMCAAPGGKTTHLAQLMKNKGTIVAVEISKTRTKALKSNINRMGVLNTIIINA-- 141

Query: 441 SMKLVKDHL 467
            M+  KD+L
Sbjct: 142 DMRKYKDYL 150
>sp|P95493|CPG2_PORGI Gingipain R2 precursor (Gingipain 2) (Arg-gingipain) (RGP-2)
          Length = 736

 Score = 30.4 bits (67), Expect = 3.1
 Identities = 23/72 (31%), Positives = 35/72 (48%), Gaps = 1/72 (1%)
 Frame = +3

Query: 108 IIVDSYGSTVNFYPDSYF-ILKTLSSHPKIEVAVASRTTEIEGAFNLIKVSGWDQFIKYK 284
           I+ D  G  VNF P  Y  + KTL  + +I VAV+      +   +L+K S    F  ++
Sbjct: 159 ILRDVRGQVVNFAPLQYNPVTKTLRIYTEIVVAVSETAEAGQNTISLVKNS---TFTGFE 215

Query: 285 EIYPGSKMTHMA 320
           +IY    M + A
Sbjct: 216 DIYKSVFMNYEA 227
>sp|Q6QEJ7|PROF_APIME Profilin
          Length = 126

 Score = 30.0 bits (66), Expect = 4.0
 Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 3/49 (6%)
 Frame = +3

Query: 333 SSGVMF--NRIIFFDDEERNVK-DVSKMGVHCVLVNSGVSMKLVKDHLQ 470
           SSGV    NR I+    +R ++  + K+GVHC+     V + L +D +Q
Sbjct: 58  SSGVTLAGNRYIYLSGTDRVIRAKLGKVGVHCMKTTQAVVVSLYEDPIQ 106
>sp|P75114|SYE_MYCPN Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
          Length = 484

 Score = 29.3 bits (64), Expect = 6.9
 Identities = 12/52 (23%), Positives = 24/52 (46%)
 Frame = +3

Query: 261 WDQFIKYKEIYPGSKMTHMAKLSRSSGVMFNRIIFFDDEERNVKDVSKMGVH 416
           W+  ++ K   PG+ +T +  L  +    +N  +  DD +  + DV +   H
Sbjct: 166 WNDQVRGKITIPGNSLTDIVLLKANGIATYNFAVVIDDHDMEITDVLRGAEH 217
>sp|P26168|BCHE_RHOCA Anaerobic magnesium-protoporphyrin IX monomethyl ester [oxidative]
           cyclase (Anaerobic Mg-protoporphyrin IX monomethyl ester
           oxidative cyclase)
          Length = 575

 Score = 28.9 bits (63), Expect = 9.0
 Identities = 15/38 (39%), Positives = 18/38 (47%)
 Frame = +3

Query: 54  WKFWIDTHVTPPFSKKNEIIVDSYGSTVNFYPDSYFIL 167
           WKFW D  V  P     + +VD     VN Y   +FIL
Sbjct: 214 WKFWRDYRVRSP-----KAVVDEIEDLVNNYDVGFFIL 246
>sp|P47700|SYE_MYCGE Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
          Length = 484

 Score = 28.9 bits (63), Expect = 9.0
 Identities = 12/52 (23%), Positives = 24/52 (46%)
 Frame = +3

Query: 261 WDQFIKYKEIYPGSKMTHMAKLSRSSGVMFNRIIFFDDEERNVKDVSKMGVH 416
           W+  ++ +   PGS +T +  L  +    +N  +  DD +  + DV +   H
Sbjct: 166 WNDLVRGQITIPGSALTDIVILKANGVATYNFAVVIDDYDMEITDVLRGAEH 217
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,494,450
Number of Sequences: 369166
Number of extensions: 1019430
Number of successful extensions: 2272
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2270
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3980545740
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)