Planarian EST Database


Dr_sW_003_J05

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_003_J05
         (372 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P59999|ARPC4_MOUSE  Actin-related protein 2/3 complex sub...   153   1e-37
sp|P58798|ARPC4_CAEEL  Probable actin-related protein 2/3 co...   140   7e-34
sp|Q92352|ARPC4_SCHPO  Probable ARP2/3 complex 20 kDa subuni...   129   2e-30
sp|P33204|ARPC4_YEAST  ARP2/3 complex 20 kDa subunit (p20-ARC)    120   7e-28
sp|P09838|TDT_MOUSE  DNA nucleotidylexotransferase (Terminal...    30   2.3  
sp|O55111|DSG2_MOUSE  Desmoglein-2 precursor                       29   2.9  
sp|P25655|NOT1_YEAST  General negative regulator of transcri...    29   2.9  
sp|O75691|DRIM_HUMAN  Down-regulated in metastasis protein (...    28   6.6  
>sp|P59999|ARPC4_MOUSE Actin-related protein 2/3 complex subunit 4 (ARP2/3 complex 20 kDa
           subunit) (p20-ARC)
 sp|P59998|ARPC4_HUMAN Actin-related protein 2/3 complex subunit 4 (ARP2/3 complex 20 kDa
           subunit) (p20-ARC)
          Length = 168

 Score =  153 bits (386), Expect = 1e-37
 Identities = 75/91 (82%), Positives = 82/91 (90%)
 Frame = +2

Query: 2   QSDEIEKLLCHKFTRFMMMRAEEFVILRRKPVQGYDISFLITNVHTEQMLKHKLVDFIIG 181
           Q+DEIEK+LCHKF RFMMMRAE F ILRRKPV+GYDISFLITN HTEQM KHKLVDF+I 
Sbjct: 78  QADEIEKILCHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHTEQMYKHKLVDFVIH 137

Query: 182 FMEDIDKEISEMRLAVNARARQCAEEFLKAF 274
           FME+IDKEISEM+L+VNARAR  AEEFLK F
Sbjct: 138 FMEEIDKEISEMKLSVNARARIVAEEFLKNF 168
>sp|P58798|ARPC4_CAEEL Probable actin-related protein 2/3 complex subunit 4 (ARP2/3
           complex 20 kDa subunit) (p20-ARC)
          Length = 169

 Score =  140 bits (354), Expect = 7e-34
 Identities = 67/92 (72%), Positives = 79/92 (85%)
 Frame = +2

Query: 2   QSDEIEKLLCHKFTRFMMMRAEEFVILRRKPVQGYDISFLITNVHTEQMLKHKLVDFIIG 181
           QSDEIEK+LCHKFTRFM  RA+ F +LRRKP+ GYDISFLIT  HTE M KHKLVDF++ 
Sbjct: 78  QSDEIEKILCHKFTRFMCQRADNFFVLRRKPLPGYDISFLITASHTEAMFKHKLVDFLLH 137

Query: 182 FMEDIDKEISEMRLAVNARARQCAEEFLKAFD 277
           FM++IDKEISEM+L++NARAR  AEEFLK F+
Sbjct: 138 FMQEIDKEISEMKLSLNARARVSAEEFLKRFN 169
>sp|Q92352|ARPC4_SCHPO Probable ARP2/3 complex 20 kDa subunit (p20-ARC)
          Length = 168

 Score =  129 bits (325), Expect = 2e-30
 Identities = 64/91 (70%), Positives = 74/91 (81%)
 Frame = +2

Query: 2   QSDEIEKLLCHKFTRFMMMRAEEFVILRRKPVQGYDISFLITNVHTEQMLKHKLVDFIIG 181
           Q DEIE++L  KF +F+M RAE F ILRRKPVQGYDISFLITN HTE+MLKHKLVDFII 
Sbjct: 78  QVDEIERILVRKFMQFLMGRAESFFILRRKPVQGYDISFLITNYHTEEMLKHKLVDFIIE 137

Query: 182 FMEDIDKEISEMRLAVNARARQCAEEFLKAF 274
           FME++D EISEM+L +N RAR  AE +L  F
Sbjct: 138 FMEEVDAEISEMKLFLNGRARLVAETYLSCF 168
>sp|P33204|ARPC4_YEAST ARP2/3 complex 20 kDa subunit (p20-ARC)
          Length = 171

 Score =  120 bits (302), Expect = 7e-28
 Identities = 61/91 (67%), Positives = 71/91 (78%)
 Frame = +2

Query: 2   QSDEIEKLLCHKFTRFMMMRAEEFVILRRKPVQGYDISFLITNVHTEQMLKHKLVDFIIG 181
           Q+DEIE++L HKFTRF+  RAE F ILRR P+ GY ISFLITN HTE M   KLVDFII 
Sbjct: 79  QADEIEQILVHKFTRFLEQRAEAFYILRRVPIPGYSISFLITNKHTESMKTGKLVDFIIE 138

Query: 182 FMEDIDKEISEMRLAVNARARQCAEEFLKAF 274
           FMED+DKEISE++L +NARAR  AE +L  F
Sbjct: 139 FMEDVDKEISEIKLFLNARARFVAEAYLDEF 169
>sp|P09838|TDT_MOUSE DNA nucleotidylexotransferase (Terminal addition enzyme) (Terminal
           deoxynucleotidyltransferase) (TDT) (Terminal
           transferase)
          Length = 530

 Score = 29.6 bits (65), Expect = 2.3
 Identities = 15/34 (44%), Positives = 20/34 (58%), Gaps = 4/34 (11%)
 Frame = +2

Query: 83  RRKPVQGYDISFLITNVHT----EQMLKHKLVDF 172
           RR  + G+D+ FLIT+       EQ L HK+ DF
Sbjct: 335 RRGKMTGHDVDFLITSPEATEDEEQQLLHKVTDF 368
>sp|O55111|DSG2_MOUSE Desmoglein-2 precursor
          Length = 1122

 Score = 29.3 bits (64), Expect = 2.9
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = -2

Query: 371 LAKINSKLLENKSINLLYKVTGK 303
           +AKI+S L E K I + YK TGK
Sbjct: 75  IAKIHSDLAEEKGIKITYKYTGK 97
>sp|P25655|NOT1_YEAST General negative regulator of transcription subunit 1
          Length = 2108

 Score = 29.3 bits (64), Expect = 2.9
 Identities = 16/50 (32%), Positives = 31/50 (62%)
 Frame = -2

Query: 371  LAKINSKLLENKSINLLYKVTGKISIIINIPNRMPSGIPQHIVELEHSLP 222
            L +  SK   + +++++YK  G + +I+ I N MPS + ++  EL ++LP
Sbjct: 1789 LDQYTSKHAVSDAVSVVYK--GTLRVILGISNDMPSFLIENHYELMNNLP 1836
>sp|O75691|DRIM_HUMAN Down-regulated in metastasis protein (Key-1A6 protein) (Novel
            nucleolar protein 73) (NNP73)
          Length = 2785

 Score = 28.1 bits (61), Expect = 6.6
 Identities = 14/55 (25%), Positives = 30/55 (54%), Gaps = 4/55 (7%)
 Frame = +2

Query: 8    DEIEKLLCHKFTRFMMMRAEEF----VILRRKPVQGYDISFLITNVHTEQMLKHK 160
            + ++ +  H+  R +   A++     V+L  K +Q Y + + +T +  E+MLKH+
Sbjct: 1576 ENMKHIQIHRRARALKKLAKQLMEGKVVLSSKSLQNYIMPYAMTPIFDEKMLKHE 1630
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,064,281
Number of Sequences: 369166
Number of extensions: 626179
Number of successful extensions: 1580
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1564
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1580
length of database: 68,354,980
effective HSP length: 90
effective length of database: 51,728,830
effective search space used: 1707051390
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)