Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_H24
(629 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P51469|G3P_XENLA Glyceraldehyde-3-phosphate dehydrogenas... 251 1e-66
sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenas... 247 2e-65
sp|Q28554|G3P_SHEEP Glyceraldehyde-3-phosphate dehydrogenas... 246 5e-65
sp|O57479|G3P_COLLI Glyceraldehyde-3-phosphate dehydrogenas... 245 8e-65
sp|P00355|G3P_PIG Glyceraldehyde-3-phosphate dehydrogenase ... 244 1e-64
sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenas... 244 1e-64
sp|Q05025|G3P_COTJA Glyceraldehyde-3-phosphate dehydrogenas... 244 2e-64
sp|P46406|G3P_RABIT Glyceraldehyde-3-phosphate dehydrogenas... 243 2e-64
sp|Q28259|G3P_CANFA Glyceraldehyde-3-phosphate dehydrogenas... 243 2e-64
sp|P00354|G3P1_HUMAN Glyceraldehyde-3-phosphate dehydrogena... 242 5e-64
>sp|P51469|G3P_XENLA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 251 bits (641), Expect = 1e-66
Identities = 123/173 (71%), Positives = 142/173 (82%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V+ND+FGI EGLMTT+HA+TATQKTVDGPS KLWRDGRGA QNIIP IP
Sbjct: 161 VINDNFGIVEGLMTTVHAFTATQKTVDGPSGKLWRDGRGAGQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGKITGMAFRVP P+VSVVDLTC+L+K AKYD+IKAAIK ASE MKGIL YT+++VV
Sbjct: 221 ELNGKITGMAFRVPTPNVSVVDLTCRLQKPAKYDDIKAAIKTASEGPMKGILGYTQDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF GD +SSIFDA AGI L + FVKLV+WYDNE GYS RV+DL+ HM++K+
Sbjct: 281 STDFNGDTHSSIFDADAGIALNENFVKLVSWYDNECGYSNRVVDLVCHMASKE 333
>sp|Q9N2D5|G3P_FELCA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 247 bits (630), Expect = 2e-65
Identities = 119/173 (68%), Positives = 140/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP IP
Sbjct: 161 VIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+KAAKYD+IK +K ASE +KGIL YTE++VV
Sbjct: 221 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKGILGYTEDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM++K+
Sbjct: 281 SCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE 333
>sp|Q28554|G3P_SHEEP Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 322
Score = 246 bits (627), Expect = 5e-65
Identities = 119/173 (68%), Positives = 139/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP IP
Sbjct: 150 VIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIP 209
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYDEIK +K ASE +KGIL YTE++VV
Sbjct: 210 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDEIKKVVKQASEGPLKGILGYTEDQVV 269
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM++K+
Sbjct: 270 SCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMASKE 322
>sp|O57479|G3P_COLLI Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 245 bits (625), Expect = 8e-65
Identities = 119/173 (68%), Positives = 141/173 (81%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++D+FGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP IP
Sbjct: 161 VIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK +K A++ +KGILAYTE++VV
Sbjct: 221 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVVKAAADGPLKGILAYTEDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF GD +SS FDA AGI L D FVKLV+WYDNEYGYS RV+DL+ HM++K+
Sbjct: 281 SCDFNGDSHSSTFDAGAGIALNDHFVKLVSWYDNEYGYSNRVVDLMVHMASKE 333
>sp|P00355|G3P_PIG Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 244 bits (624), Expect = 1e-64
Identities = 118/173 (68%), Positives = 139/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP IP
Sbjct: 161 VIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK +K ASE +KGIL YTE++VV
Sbjct: 221 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM++K+
Sbjct: 281 SCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMASKE 333
>sp|P20287|G3P_SCHMA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Major larval
surface antigen) (P-37)
Length = 338
Score = 244 bits (623), Expect = 1e-64
Identities = 119/174 (68%), Positives = 136/174 (78%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++D F I EGLMTT+H++TATQK VDGPSSKLWRDGRGA QNIIP IP
Sbjct: 164 VIHDKFEIVEGLMTTVHSFTATQKVVDGPSSKLWRDGRGAMQNIIPASTGAAKAVGKVIP 223
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
LNGK+TGMAFRVP PDVSVVDLTC+L K A Y+EIKAA+K A+ +KGIL YTE+EVV
Sbjct: 224 ALNGKLTGMAFRVPTPDVSVVDLTCRLGKGASYEEIKAAVKAAASGPLKGILEYTEDEVV 283
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKDH 523
S DF G +SSIFDAKAGI L + FVKLV+WYDNE+GYSCRV+DLI HM DH
Sbjct: 284 SSDFVGSTSSSIFDAKAGISLNNNFVKLVSWYDNEFGYSCRVVDLITHMHKVDH 337
>sp|Q05025|G3P_COTJA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 244 bits (622), Expect = 2e-64
Identities = 118/173 (68%), Positives = 140/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++D+FGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP IP
Sbjct: 161 VIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+K AKYD+IK +K A+E +KGIL YTE++VV
Sbjct: 221 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKRVVKAAAEGPLKGILGYTEDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF GD +SS FDA AGI L D FVKLV+WYDNE+GYS RV+DL+ HM++K+
Sbjct: 281 SCDFNGDSHSSTFDAGAGIALNDNFVKLVSWYDNEFGYSNRVVDLMVHMASKE 333
>sp|P46406|G3P_RABIT Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 243 bits (621), Expect = 2e-64
Identities = 118/173 (68%), Positives = 139/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++DHFGI EGLMTT+HA TATQKTVDGPS KLWRDGRGAAQNIIP IP
Sbjct: 161 VIHDHFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+KAAKYD+IK +K ASE +KGIL YTE++VV
Sbjct: 221 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKGILGYTEDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ HM++K+
Sbjct: 281 SCDFNSATHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVHMASKE 333
>sp|Q28259|G3P_CANFA Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
Length = 333
Score = 243 bits (621), Expect = 2e-64
Identities = 117/173 (67%), Positives = 140/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++DHFGI EGLMTT+HA TATQKTVDGPS K+WRDGRGAAQNIIP IP
Sbjct: 161 VIHDHFGIVEGLMTTVHAITATQKTVDGPSGKMWRDGRGAAQNIIPASTGAAKAVGKVIP 220
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
ELNGK+TGMAFRVP P+VSVVDLTC+L+KAAKYD+IK +K ASE +KGIL YTE++VV
Sbjct: 221 ELNGKLTGMAFRVPTPNVSVVDLTCRLEKAAKYDDIKKVVKQASEGPLKGILGYTEDQVV 280
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF D +SS FDA AGI L D FVKL++WYDNE+GYS RV+DL+ +M++K+
Sbjct: 281 SCDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMVYMASKE 333
>sp|P00354|G3P1_HUMAN Glyceraldehyde-3-phosphate dehydrogenase, muscle (GAPDH)
Length = 334
Score = 242 bits (618), Expect = 5e-64
Identities = 116/173 (67%), Positives = 140/173 (80%)
Frame = +2
Query: 2 VVNDHFGIEEGLMTTIHAYTATQKTVDGPSSKLWRDGRGAAQNIIPXXXXXXXXXXXXIP 181
V++DHFGI EGLMTT+HA TATQKTVD PS KLWR GRGAAQN+IP IP
Sbjct: 162 VIHDHFGIVEGLMTTVHAITATQKTVDSPSGKLWRGGRGAAQNLIPASTGAAKAVGKVIP 221
Query: 182 ELNGKITGMAFRVPVPDVSVVDLTCKLKKAAKYDEIKAAIKHASETTMKGILAYTEEEVV 361
EL+GK+TGMAFRVP +VSV+DLTC+L+K AKYD+IK +K ASE +KGIL YTE+EVV
Sbjct: 222 ELDGKLTGMAFRVPTANVSVLDLTCRLEKPAKYDDIKKVVKEASEGPLKGILGYTEDEVV 281
Query: 362 SQDFKGDHNSSIFDAKAGIQLTDTFVKLVAWYDNEYGYSCRVIDLIQHMSTKD 520
S DF G ++SSIFDA AGI+L DTFVKLV+WYDNE+GYS RV+DL+ HM++K+
Sbjct: 282 SDDFNGSNHSSIFDAGAGIELNDTFVKLVSWYDNEFGYSERVVDLMAHMASKE 334
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,744,274
Number of Sequences: 369166
Number of extensions: 1281716
Number of successful extensions: 3455
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3249
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3324
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5023626210
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)