Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_003_G08 (891 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q8K991|TIG_BUCAP Trigger factor (TF) 33 1.4 sp|P40107|GOG5_YEAST GDP-mannose transporter 31 5.3 sp|P57546|TIG_BUCAI Trigger factor (TF) 30 6.9 sp|P47637|Y397_MYCGE Hypothetical protein MG397 30 9.0
>sp|Q8K991|TIG_BUCAP Trigger factor (TF) Length = 442 Score = 32.7 bits (73), Expect = 1.4 Identities = 30/136 (22%), Positives = 59/136 (43%), Gaps = 3/136 (2%) Frame = -1 Query: 675 QTRRKLHKKIKFISLKLVKRIIF---KNCLLQKCHKNAFQMYLKWKIIEIISQLVVSSKF 505 Q ++ K IK I + + ++ + KN +++K K+ Q +L+ +II QL++ + Sbjct: 246 QENIEIEKNIKTIKINKLSKLNYQTIKNNIIEKI-KSLTQNHLQ---NQIIKQLIIKNPI 301 Query: 504 KITAFIVVNNISITTQKIIQSYKHTQEVTQIDRAI*TVQEMTVLKNSYRKP*ADVNLHTR 325 I ++ + K I+ YK QE +LK Y NL ++ Sbjct: 302 NIPPTLLREETNFLRNKFIKEYKEKQE--------------NILKKKYH-----TNLESK 342 Query: 324 FLTMKYFKLFVKLCVK 277 T + KL ++ ++ Sbjct: 343 AKTRLHIKLIIEKIIR 358
>sp|P40107|GOG5_YEAST GDP-mannose transporter Length = 337 Score = 30.8 bits (68), Expect = 5.3 Identities = 18/48 (37%), Positives = 29/48 (60%) Frame = +2 Query: 278 FTHNLTNNLKYFIVRKRVCKLTSAYGFR*EFFNTVISCTV*IARSICV 421 FT+ +T+ L I+RKR+ KLT+ F F+N V++ + + S CV Sbjct: 189 FTNCITSALFVLIMRKRI-KLTNFKDFDTMFYNNVLALPILLLFSFCV 235
>sp|P57546|TIG_BUCAI Trigger factor (TF) Length = 442 Score = 30.4 bits (67), Expect = 6.9 Identities = 30/122 (24%), Positives = 54/122 (44%), Gaps = 36/122 (29%) Frame = -1 Query: 669 RRKLHKKI-----KFISLKLVKRIIFKNCLL------QKCHKNAFQMYLKW------KII 541 + LH +I K++ +++++I+ KN LL QK KN ++ Y K I+ Sbjct: 272 KNNLHSQINIITDKYLENQIIQKIVEKNILLLPPLLFQKEIKNLYKQYTKQYQEENSNIL 331 Query: 540 E-------------------IISQLVVSSKFKITAFIVVNNISITTQKIIQSYKHTQEVT 418 E II Q+++++K F NNI +KI +YK+ E+ Sbjct: 332 EKKYHMSLDSEVKKRLYFQIIIEQIILNNKL----FADENNIQKLIKKISSNYKNPMEII 387 Query: 417 QI 412 ++ Sbjct: 388 KL 389
>sp|P47637|Y397_MYCGE Hypothetical protein MG397 Length = 566 Score = 30.0 bits (66), Expect = 9.0 Identities = 32/92 (34%), Positives = 43/92 (46%), Gaps = 3/92 (3%) Frame = -1 Query: 687 LINTQTRRKLHKKIKFISLKLVKRIIFKNCL-LQKCHKNAFQMYLKWKIIEIISQL--VV 517 +IN+Q KLH+ I I++KL F N L L C +N + + E IS L V Sbjct: 249 VINSQDLLKLHELINNITIKLKDCTNFLNNLNLMLCERNMDE------VQEAISWLFQAV 302 Query: 516 SSKFKITAFIVVNNISITTQKIIQSYKHTQEV 421 SSK K +V N Q IQ Y+ E+ Sbjct: 303 SSKEKSINLVVENIDDFRNQ--IQQYEKKNEL 332
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 95,904,016 Number of Sequences: 369166 Number of extensions: 1799907 Number of successful extensions: 3279 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 3202 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3278 length of database: 68,354,980 effective HSP length: 110 effective length of database: 48,034,130 effective search space used: 8934348180 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)