Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_F16
(758 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P11974|KPYM_RABIT Pyruvate kinase, isozymes M1/M2 (Pyruv... 349 5e-96
sp|P00548|KPYK_CHICK Pyruvate kinase, muscle isozyme 348 9e-96
sp|Q92122|KPYK_XENLA Pyruvate kinase, muscle isozyme (Cytos... 347 2e-95
sp|P52480|KPYM_MOUSE Pyruvate kinase, isozyme M2 347 3e-95
sp|P14618|KPYM_HUMAN Pyruvate kinase, isozymes M1/M2 (Pyruv... 344 1e-94
sp|P11979|KPYM_FELCA Pyruvate kinase, isozyme M1 (Pyruvate ... 344 2e-94
sp|P11980|KPYM_RAT Pyruvate kinase, isozymes M1/M2 (Pyruvat... 343 3e-94
sp|O62619|KPYK_DROME Pyruvate kinase (PK) 317 3e-86
sp|P30613|KPYR_HUMAN Pyruvate kinase, isozymes R/L (R-type/... 304 2e-82
sp|P12928|KPYR_RAT Pyruvate kinase, isozymes R/L (L-PK) 291 2e-78
>sp|P11974|KPYM_RABIT Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
Length = 531
Score = 349 bits (895), Expect = 5e-96
Identities = 177/254 (69%), Positives = 205/254 (80%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDS P R++ IICTIGPA R+V TLK+MI SGMN+ARMNFSHG+H
Sbjct: 22 MADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVETLKEMIKSGMNVARMNFSHGTH 81
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAETIK VR A E+F YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG +K
Sbjct: 82 EYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLK 141
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D Y E C+E ILW+DYKNI K+++ G ++YVDDGLISL V++KGPDFL EVENGG
Sbjct: 142 ITLDNAYMEKCDENILWLDYKNICKVVDVGSKVYVDDGLISLQVKQKGPDFLVTEVENGG 201
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS+KDI DL FGVEQ VDMVFASFIR A V E+R ILGEK
Sbjct: 202 FLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKILGEK 261
Query: 716 GKHILIISKIENHE 757
GK+I IISKIENHE
Sbjct: 262 GKNIKIISKIENHE 275
>sp|P00548|KPYK_CHICK Pyruvate kinase, muscle isozyme
Length = 530
Score = 348 bits (893), Expect = 9e-96
Identities = 172/254 (67%), Positives = 206/254 (81%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDSEP + R++ IICTIGPA R+V+ LK+MI SGMN+AR+NFSHG+H
Sbjct: 21 MADTFLEHMCRLDIDSEPTIARNTGIICTIGPASRSVDKLKEMIKSGMNVARLNFSHGTH 80
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYH TIK VREA E+F YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG +K
Sbjct: 81 EYHEGTIKNVREATESFASDPITYRPVAIALDTKGPEIRTGLIKGSGTAEVELKKGAALK 140
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D + E C+E +LWVDYKN++K+++ G +IYVDDGLISL+V+EKG DF+ EVENGG
Sbjct: 141 VTLDNAFMENCDENVLWVDYKNLIKVIDVGSKIYVDDGLISLLVKEKGKDFVMTEVENGG 200
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS+KDI DL FGVEQ VDMVFASFIR A V +R +LGEK
Sbjct: 201 MLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQNVDMVFASFIRKAADVHAVRKVLGEK 260
Query: 716 GKHILIISKIENHE 757
GKHI IISKIENHE
Sbjct: 261 GKHIKIISKIENHE 274
>sp|Q92122|KPYK_XENLA Pyruvate kinase, muscle isozyme (Cytosolic thyroid hormone binding
protein) (CTHBP)
Length = 527
Score = 347 bits (890), Expect = 2e-95
Identities = 173/254 (68%), Positives = 205/254 (80%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDSEP V R++ IICTIGPA R+V LK+MI SGMNIAR+NFSHG+H
Sbjct: 18 MADTFLEHMCRLDIDSEPIVARNTGIICTIGPASRSVEMLKEMIKSGMNIARLNFSHGTH 77
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHA TIK VREA E+ +YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG ++
Sbjct: 78 EYHAGTIKNVREATESLASNPIHYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATMR 137
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D+ + E C+E +LWVDYKN+ K++ PG +IYVDDGLISL+V+E GPDF E+ENGG
Sbjct: 138 ITLDDAFQENCDENVLWVDYKNLTKVVKPGSKIYVDDGLISLLVKEIGPDFCVTEIENGG 197
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS KDI DL FGVEQ VDMVFASFIR A V E+R +LGEK
Sbjct: 198 MLGSKKGVNLPGAAVDLPAVSSKDIQDLQFGVEQDVDMVFASFIRKAADVHEVREVLGEK 257
Query: 716 GKHILIISKIENHE 757
GK+I IISKIENHE
Sbjct: 258 GKNIKIISKIENHE 271
>sp|P52480|KPYM_MOUSE Pyruvate kinase, isozyme M2
Length = 531
Score = 347 bits (889), Expect = 3e-95
Identities = 176/254 (69%), Positives = 203/254 (79%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDS P R++ IICTIGPA R+V LK+MI SGMN+AR+NFSHG+H
Sbjct: 22 MADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTH 81
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAETIK VREA E+F YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG +K
Sbjct: 82 EYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLK 141
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D Y E C+E ILW+DYKNI K++ G +IYVDDGLISL V+EKG DFL EVENGG
Sbjct: 142 ITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGG 201
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS+KDI DL FGVEQ VDMVFASFIR A V E+R +LGEK
Sbjct: 202 SLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEK 261
Query: 716 GKHILIISKIENHE 757
GK+I IISKIENHE
Sbjct: 262 GKNIKIISKIENHE 275
>sp|P14618|KPYM_HUMAN Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
(Cytosolic thyroid hormone-binding protein) (CTHBP)
(THBP1)
Length = 531
Score = 344 bits (883), Expect = 1e-94
Identities = 174/254 (68%), Positives = 203/254 (79%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDS P R++ IICTIGPA R+V TLK+MI SGMN+AR+NFSHG+H
Sbjct: 22 MADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTH 81
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAETIK VR A E+F YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG +K
Sbjct: 82 EYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLK 141
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D Y E C+E ILW+DYKNI K++ G +IYVDDGLISL V++KG DFL EVENGG
Sbjct: 142 ITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGG 201
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS+KDI DL FGVEQ VDMVFASFIR + V E+R +LGEK
Sbjct: 202 SLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEK 261
Query: 716 GKHILIISKIENHE 757
GK+I IISKIENHE
Sbjct: 262 GKNIKIISKIENHE 275
>sp|P11979|KPYM_FELCA Pyruvate kinase, isozyme M1 (Pyruvate kinase muscle isozyme)
Length = 531
Score = 344 bits (882), Expect = 2e-94
Identities = 172/254 (67%), Positives = 203/254 (79%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDS P R++ IICTIGPA R+V LK+MI SGMN+AR+NFSHG+H
Sbjct: 22 MADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVEILKEMIKSGMNVARLNFSHGTH 81
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAETIK VR A E+F YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG +K
Sbjct: 82 EYHAETIKNVRAATESFASDPIRYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLK 141
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D Y E C+E +LW+DYKNI K++ G ++YVDDGLISL+V+EKG DFL EVENGG
Sbjct: 142 ITLDNAYMEKCDENVLWLDYKNICKVVEVGSKVYVDDGLISLLVKEKGADFLVTEVENGG 201
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS+KDI DL FGVEQ VDMVFASFIR + V E+R +LGEK
Sbjct: 202 SLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEK 261
Query: 716 GKHILIISKIENHE 757
GK+I IISKIENHE
Sbjct: 262 GKNIKIISKIENHE 275
>sp|P11980|KPYM_RAT Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
Length = 531
Score = 343 bits (880), Expect = 3e-94
Identities = 174/254 (68%), Positives = 202/254 (79%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDS P R++ IICTIGPA R+V LK+MI SGMN+AR+NFSHG+H
Sbjct: 22 MADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTH 81
Query: 185 EYHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAETIK VR A E+F YRPVA+ALDTKGPEIRTGLI GS TAE+ L KG +K
Sbjct: 82 EYHAETIKNVRAATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLK 141
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D Y E C+E ILW+DYKNI K++ G +IYVDDGLISL V+EKG D+L EVENGG
Sbjct: 142 ITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADYLVTEVENGG 201
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGSKKGVNLPGA VDLPAVS+KDI DL FGVEQ VDMVFASFIR A V E+R +LGEK
Sbjct: 202 SLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEK 261
Query: 716 GKHILIISKIENHE 757
GK+I IISKIENHE
Sbjct: 262 GKNIKIISKIENHE 275
>sp|O62619|KPYK_DROME Pyruvate kinase (PK)
Length = 533
Score = 317 bits (811), Expect = 3e-86
Identities = 163/253 (64%), Positives = 198/253 (78%), Gaps = 3/253 (1%)
Frame = +2
Query: 8 ARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSHE 187
A + LE++C L DS VR S I+CTIGPA +V L+KM+A+GMNIARMNFSHGSHE
Sbjct: 26 ADTQLEHMCRLQFDSPVPHVRLSGIVCTIGPASSSVEMLEKMMATGMNIARMNFSHGSHE 85
Query: 188 YHAETIKLVREAVETFTP---YYRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIKM 358
YHA T+ VR+AV+ ++ Y PVA+ALDTKGPEIRTGLI GS TAEI L KG KIK+
Sbjct: 86 YHAATVANVRQAVKNYSAKLGYEHPVAIALDTKGPEIRTGLIGGSGTAEIELKKGEKIKL 145
Query: 359 VIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGGK 538
++++ E + EI++VDY+NIV ++ PG R++VDDGLISLIV+E G D L CEVENGG
Sbjct: 146 TTNKEFLEKGSLEIVYVDYENIVNVVKPGNRVFVDDGLISLIVREVGKDSLTCEVENGGS 205
Query: 539 LGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEKG 718
LGS+KGVNLPG VDLPAVS+KD SDL FGVEQ VDM+FASFIR AA+ EIR +LGEKG
Sbjct: 206 LGSRKGVNLPGVPVDLPAVSEKDKSDLLFGVEQEVDMIFASFIRNAAALTEIRKVLGEKG 265
Query: 719 KHILIISKIENHE 757
K+I IISKIEN +
Sbjct: 266 KNIKIISKIENQQ 278
>sp|P30613|KPYR_HUMAN Pyruvate kinase, isozymes R/L (R-type/L-type pyruvate kinase) (Red
cell/liver pyruvate kinase)
Length = 574
Score = 304 bits (778), Expect = 2e-82
Identities = 152/254 (59%), Positives = 196/254 (77%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDSEP RS+SII TIGPA R+V LK+MI +GMNIAR+NFSHGSH
Sbjct: 65 MADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSH 124
Query: 185 EYHAETIKLVREAVETFTPY---YRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAE+I VREAVE+F YRPVA+ALDTKGPEIRTG++ G +E+ L+KG+++
Sbjct: 125 EYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVL 184
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D + N +WVDY NIV+++ G RIY+DDGLISL+VQ+ GP+ L +VENGG
Sbjct: 185 VTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGG 244
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGS+KGVNLPGA+VDLP +S++D+ DL FGVE GVD+VFASF+R + V +R+ LG +
Sbjct: 245 VLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPE 304
Query: 716 GKHILIISKIENHE 757
G I IISKIENHE
Sbjct: 305 GHGIKIISKIENHE 318
>sp|P12928|KPYR_RAT Pyruvate kinase, isozymes R/L (L-PK)
Length = 574
Score = 291 bits (744), Expect = 2e-78
Identities = 142/254 (55%), Positives = 195/254 (76%), Gaps = 3/254 (1%)
Frame = +2
Query: 5 IARSHLEYICNLDIDSEPDVVRSSSIICTIGPACRNVNTLKKMIASGMNIARMNFSHGSH 184
+A + LE++C LDIDS+P RS+SII TIGPA R+V+ LK+MI +GMNIAR+NFSHGSH
Sbjct: 65 MADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSH 124
Query: 185 EYHAETIKLVREAVETFTPY---YRPVALALDTKGPEIRTGLINGSATAEISLIKGNKIK 355
EYHAE+I +REA E+F YRPVA+ALDTKGPEIRTG++ G +E+ ++KG+++
Sbjct: 125 EYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVL 184
Query: 356 MVIDEKYAEACNEEILWVDYKNIVKILNPGCRIYVDDGLISLIVQEKGPDFLDCEVENGG 535
+ +D K+ + + +WVDY NI +++ G RIY+DDGLISL+VQ+ GP+ L EVE+GG
Sbjct: 185 VTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGG 244
Query: 536 KLGSKKGVNLPGAKVDLPAVSDKDISDLHFGVEQGVDMVFASFIRCGAAVDEIRSILGEK 715
LGS+KGVNLP +VDLP +S++D+ DL FGV+ VD++FASF+R + V +R LG +
Sbjct: 245 ILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPE 304
Query: 716 GKHILIISKIENHE 757
G++I IISKIENHE
Sbjct: 305 GQNIKIISKIENHE 318
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 82,711,858
Number of Sequences: 369166
Number of extensions: 1623620
Number of successful extensions: 5325
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4939
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5154
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6970118400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)