Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_E18
(749 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q28034|GLU2B_BOVIN Glucosidase II beta subunit precursor... 163 4e-40
sp|O08795|GLU2B_MOUSE Glucosidase II beta subunit precursor... 162 9e-40
sp|P14314|GLU2B_HUMAN Glucosidase II beta subunit precursor... 159 8e-39
sp|Q9USH8|YJQ2_SCHPO Hypothetical protein C825.02 in chromo... 70 8e-12
sp|Q58CS8|GNPTG_BOVIN N-acetylglucosamine-1-phosphotransfer... 66 9e-11
sp|Q9UJJ9|GNPTG_HUMAN N-acetylglucosamine-1-phosphotransfer... 65 1e-10
sp|Q6S5C2|GNPTG_MOUSE N-acetylglucosamine-1-phosphotransfer... 64 4e-10
sp|P16025|RPOC2_MAIZE DNA-directed RNA polymerase beta'' ch... 35 0.28
sp|P52025|DPOL_METVO DNA polymerase 34 0.37
sp|P44496|RECN_HAEIN DNA repair protein recN (Recombination... 33 0.82
>sp|Q28034|GLU2B_BOVIN Glucosidase II beta subunit precursor (Protein kinase C substrate,
60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein)
(Vacuolar system associated protein 60) (VASAP-60)
Length = 533
Score = 163 bits (413), Expect = 4e-40
Identities = 83/204 (40%), Positives = 111/204 (54%), Gaps = 3/204 (1%)
Frame = +2
Query: 2 DFDGAVPDPVDQAPEDEKTLEETPHEESPKMEFDEETKKLISIANDARNNFNELNEKYMK 181
D D P D P +P EE +DE+T+ I+ A +ARN F E
Sbjct: 332 DEDSQGEQPKDAPPPAPAPQTASPTEEDRMPPYDEQTQAFINAAQEARNKFEEAERSLKD 391
Query: 182 LENEKKSIESLLNIDYGPNSAFYKLAEDDTCYELTDREYIYKLCPFKKTSQMSKDSGPET 361
+E +++E ++ D+GPN F L CYELT EY+Y+LCPFK SQ K G T
Sbjct: 392 MEESIRNLEQEISFDFGPNGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPT 449
Query: 362 ELGKWGHWSG-EENQYSMQIYDNGLPCWNGPNRQTKVIISCGVENKLTEASEPSRCEYLF 538
LG WG W+G + +++S Y+ G CW GPNR T V + CG E +T +EPSRCEYL
Sbjct: 450 SLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSRCEYLM 509
Query: 539 KFETPSACSKSPDQLLEE--HDEL 604
+ TP+AC + P + E HDEL
Sbjct: 510 ELMTPAACPEPPPEYPVEGDHDEL 533
>sp|O08795|GLU2B_MOUSE Glucosidase II beta subunit precursor (Protein kinase C substrate,
60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein)
Length = 521
Score = 162 bits (410), Expect = 9e-40
Identities = 81/209 (38%), Positives = 117/209 (55%), Gaps = 18/209 (8%)
Frame = +2
Query: 32 DQAPEDEKTLEETPHEESPKME---------------FDEETKKLISIANDARNNFNELN 166
++ PE+E+ EE E P ++ +DEET+ +I A +AR+ F E+
Sbjct: 315 EEEPEEEEEEEEEEEEAPPPLQPPQPPSPTEDEKMPPYDEETQAIIDAAQEARSKFEEVE 374
Query: 167 EKYMKLENEKKSIESLLNIDYGPNSAFYKLAEDDTCYELTDREYIYKLCPFKKTSQMSKD 346
++E +S+E ++ D+GP+ F L CYELT EY+Y+LCPFK SQ K
Sbjct: 375 RSLKEMEESIRSLEQEISFDFGPSGEFAYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKH 432
Query: 347 SGPETELGKWGHWSG-EENQYSMQIYDNGLPCWNGPNRQTKVIISCGVENKLTEASEPSR 523
G T LG WG W+G + +++S Y+ G CW GPNR T V + CG E +T +EPSR
Sbjct: 433 GGSPTSLGTWGSWAGPDHDKFSAMKYEQGTGCWQGPNRSTTVRLLCGKETVVTSTTEPSR 492
Query: 524 CEYLFKFETPSACSKSPDQLLE--EHDEL 604
CEYL + TP+AC + P + +HDEL
Sbjct: 493 CEYLMELMTPAACPEPPPEAPSDGDHDEL 521
>sp|P14314|GLU2B_HUMAN Glucosidase II beta subunit precursor (Protein kinase C substrate,
60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein)
Length = 527
Score = 159 bits (402), Expect = 8e-39
Identities = 77/182 (42%), Positives = 105/182 (57%), Gaps = 3/182 (1%)
Frame = +2
Query: 68 TPHEESPKMEFDEETKKLISIANDARNNFNELNEKYMKLENEKKSIESLLNIDYGPNSAF 247
+P EE +DE+T+ I A +ARN F E +E +++E ++ D+GPN F
Sbjct: 348 SPAEEDKMPPYDEQTQAFIDAAQEARNKFEEAERSLKDMEESIRNLEQEISFDFGPNGEF 407
Query: 248 YKLAEDDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWSG-EENQYSMQIYD 424
L CYELT EY+Y+LCPFK SQ K G T LG WG W G + +++S Y+
Sbjct: 408 AYLYSQ--CYELTTNEYVYRLCPFKLVSQKPKLGGSPTSLGTWGSWIGPDHDKFSAMKYE 465
Query: 425 NGLPCWNGPNRQTKVIISCGVENKLTEASEPSRCEYLFKFETPSACSKSPDQ--LLEEHD 598
G CW GPNR T V + CG E +T +EPSRCEYL + TP+AC + P + ++HD
Sbjct: 466 QGTGCWQGPNRSTTVRLLCGKETMVTSTTEPSRCEYLMELMTPAACPEPPPEAPTEDDHD 525
Query: 599 EL 604
EL
Sbjct: 526 EL 527
>sp|Q9USH8|YJQ2_SCHPO Hypothetical protein C825.02 in chromosome III precursor
Length = 506
Score = 69.7 bits (169), Expect = 8e-12
Identities = 29/55 (52%), Positives = 38/55 (69%)
Frame = +2
Query: 419 YDNGLPCWNGPNRQTKVIISCGVENKLTEASEPSRCEYLFKFETPSACSKSPDQL 583
Y+NG CWNGP+R V + CGVEN++ E +CEYL K ++P+AC SPDQL
Sbjct: 425 YENGQSCWNGPHRSAIVTVECGVENEIVSVLEAQKCEYLIKMKSPAAC--SPDQL 477
>sp|Q58CS8|GNPTG_BOVIN N-acetylglucosamine-1-phosphotransferase, gamma subunit precursor
(GlcNac-1-phosphotransferase-gamma subunit)
(UDP-N-acetylglucosamine-1-phosphotransferase, gamma
subunit)
Length = 306
Score = 66.2 bits (160), Expect = 9e-11
Identities = 35/100 (35%), Positives = 48/100 (48%), Gaps = 3/100 (3%)
Frame = +2
Query: 272 CYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWSGEENQYSMQIYDNGLPCW 442
C+ L + Y Y+LCPF +Q + + LG W W N + +G C
Sbjct: 71 CFSLVESTYKYELCPFHNVTQHEQTFRWNAYSGILGIWHEWEITNNTFRGMWMRDGDAC- 129
Query: 443 NGPNRQTKVIISCGVENKLTEASEPSRCEYLFKFETPSAC 562
+RQ+KV ++CG N+L SEPS C Y FETP C
Sbjct: 130 QSRSRQSKVELTCGKSNRLAHVSEPSTCVYALTFETPLVC 169
>sp|Q9UJJ9|GNPTG_HUMAN N-acetylglucosamine-1-phosphotransferase, gamma subunit precursor
(GlcNAc-1-phosphotransferase gamma subunit)
(UDP-N-acetylglucosamine-1-phosphotransferase, gamma
subunit)
Length = 305
Score = 65.5 bits (158), Expect = 1e-10
Identities = 34/100 (34%), Positives = 48/100 (48%), Gaps = 3/100 (3%)
Frame = +2
Query: 272 CYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWSGEENQYSMQIYDNGLPCW 442
C+ L + Y Y+ CPF +Q + + LG W W N ++ +G C
Sbjct: 71 CFSLVESTYKYEFCPFHNVTQHEQTFRWNAYSGILGIWHEWEIANNTFTGMWMRDGDAC- 129
Query: 443 NGPNRQTKVIISCGVENKLTEASEPSRCEYLFKFETPSAC 562
+RQ+KV ++CG N+L SEPS C Y FETP C
Sbjct: 130 RSRSRQSKVELACGKSNRLAHVSEPSTCVYALTFETPLVC 169
>sp|Q6S5C2|GNPTG_MOUSE N-acetylglucosamine-1-phosphotransferase, gamma subunit precursor
(GlcNAc-1-phosphotransferase gamma subunit)
(UDP-N-acetylglucosamine-1-phosphotransferase, gamma
subunit) (M6PR domain containing protein 1)
Length = 307
Score = 63.9 bits (154), Expect = 4e-10
Identities = 34/100 (34%), Positives = 48/100 (48%), Gaps = 3/100 (3%)
Frame = +2
Query: 272 CYELTDREYIYKLCPFKKTSQMSKD---SGPETELGKWGHWSGEENQYSMQIYDNGLPCW 442
C+ L + Y Y+ CPF +Q + + LG W W N + +G C
Sbjct: 71 CFSLVESTYKYEFCPFHNVTQHEQTFRWNAYSGILGIWHEWEIINNTFKGMWMTDGDSC- 129
Query: 443 NGPNRQTKVIISCGVENKLTEASEPSRCEYLFKFETPSAC 562
+ +RQ+KV ++CG N+L SEPS C Y FETP C
Sbjct: 130 HSRSRQSKVELTCGKINRLAHVSEPSTCVYALTFETPLVC 169
>sp|P16025|RPOC2_MAIZE DNA-directed RNA polymerase beta'' chain (PEP) (Plastid-encoded RNA
polymerase beta'' subunit) (RNA polymerase beta''
subunit)
Length = 1527
Score = 34.7 bits (78), Expect = 0.28
Identities = 28/120 (23%), Positives = 50/120 (41%)
Frame = +2
Query: 44 EDEKTLEETPHEESPKMEFDEETKKLISIANDARNNFNELNEKYMKLENEKKSIESLLNI 223
ED + E+P + E + E K L + + L ++Y LE+E +++E
Sbjct: 662 EDSEDEYESPENKYRTREGEGEYKILEDEYRTLEDEYETLEDEYGILEDEYRTLEKDSEE 721
Query: 224 DYGPNSAFYKLAEDDTCYELTDREYIYKLCPFKKTSQMSKDSGPETELGKWGHWSGEENQ 403
+YG Y+ E + YE+ + E + S + G E E G S E+++
Sbjct: 722 EYGSLENKYRTREGEGEYEILEEE--------SEEEYGSSEDGSEKEYGTLEEDSEEDSE 773
>sp|P52025|DPOL_METVO DNA polymerase
Length = 824
Score = 34.3 bits (77), Expect = 0.37
Identities = 22/56 (39%), Positives = 32/56 (57%), Gaps = 7/56 (12%)
Frame = +2
Query: 155 NELNEKYMKLENEKKSIESLLNIDYG----PNSAFYKLAEDDTCYELT---DREYI 301
NE +E+Y +LE+E+KSI+ L N YG P + +Y D C E+ R+YI
Sbjct: 509 NEFDEEYSRLEHEQKSIKVLANSHYGYLAFPMARWY----SDKCAEMVTGLGRKYI 560
>sp|P44496|RECN_HAEIN DNA repair protein recN (Recombination protein N)
Length = 558
Score = 33.1 bits (74), Expect = 0.82
Identities = 20/73 (27%), Positives = 36/73 (49%)
Frame = +2
Query: 53 KTLEETPHEESPKMEFDEETKKLISIANDARNNFNELNEKYMKLENEKKSIESLLNIDYG 232
K L+ K+ +E K+L+ D + FN +Y++LE+E++ + S +
Sbjct: 170 KNLQNQVKTFQQKVAENEARKQLLQYQVDELDEFNLRPNEYLELEDEQRRLSSSEQLTQL 229
Query: 233 PNSAFYKLAEDDT 271
SA L+E+DT
Sbjct: 230 SQSALQILSENDT 242
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,611,566
Number of Sequences: 369166
Number of extensions: 1594026
Number of successful extensions: 5485
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5004
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5396
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6824907600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)