Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_E09
(651 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P35749|MYH11_HUMAN Myosin-11 (Myosin heavy chain, smooth... 40 0.007
sp|Q15696|U2AFM_HUMAN U2 small nuclear ribonucleoprotein au... 34 0.29
sp|Q15695|U2AFL_HUMAN U2 small nuclear ribonucleoprotein au... 33 0.84
sp|Q62377|U2AFM_MOUSE U2 small nuclear ribonucleoprotein au... 32 1.1
sp|Q64707|U2AFL_MOUSE U2 small nuclear ribonucleoprotein au... 31 2.4
sp|P21192|ACE2_YEAST Metallothionein expression activator 31 3.2
sp|O44952|LONM_CAEEL Lon protease homolog, mitochondrial pr... 30 4.2
sp|Q8MIT6|ROCK1_BOVIN Rho-associated protein kinase 1 (Rho-... 30 4.2
sp|Q9PLL9|TIG_CHLMU Trigger factor (TF) 30 4.2
sp|P39922|MYS3_HYDAT Myosin heavy chain, clone 203 30 5.4
>sp|P35749|MYH11_HUMAN Myosin-11 (Myosin heavy chain, smooth muscle isoform) (SMMHC)
Length = 1972
Score = 39.7 bits (91), Expect = 0.007
Identities = 40/197 (20%), Positives = 79/197 (40%), Gaps = 6/197 (3%)
Frame = +1
Query: 76 ENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAEDKITSXXXXXXXXXXXX 255
E E Q ++LQ T E + E + LE+KHS+ + + ++++ +
Sbjct: 851 EEEMQAKEDELQKTKERQQKAENELKELEQKHSQLTEEKNLLQEQLQAETELYAEAEEMR 910
Query: 256 XXXXXXXXXXXTIRNATMALLEEEQLAKVQLE---KLLEQQNDTLHTEMQKSQNFAEALS 426
I + A LEEE+ QL+ K + QQ L ++++ + + L
Sbjct: 911 VRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQ 970
Query: 427 AEKLRISXXXXXXXXXXXXXXDSQKKMQKSGIRVKNLDSKVSRIAENVFGKED---NRIP 597
EK+ D K+ K K L+ ++S + N+ +E+ N
Sbjct: 971 LEKVTAEAKIKKLEDEILVMDDQNNKLSKER---KLLEERISDLTTNLAEEEEKAKNLTK 1027
Query: 598 VSHRGQGFIAEVMVEAR 648
+ ++ + I+E+ V +
Sbjct: 1028 LKNKHESMISELEVRLK 1044
Score = 31.2 bits (69), Expect = 2.4
Identities = 42/180 (23%), Positives = 70/180 (38%), Gaps = 14/180 (7%)
Frame = +1
Query: 73 IENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQK-------MLSQAEDKITSXXXX 231
+E++K+ L Q+Q + S E+ R L +K + Q ML++AE K
Sbjct: 1250 VEHKKKKLEAQVQELQSKCSDGERARAELNDKVHKLQNEVESVTGMLNEAEGKAIKLAKD 1309
Query: 232 XXXXXXXXXXXXXXXXXXXTIRNATMALLEEEQLAKV----QLEKLLEQQN---DTLHTE 390
T LL+EE K+ +L +L E++N D L E
Sbjct: 1310 VASLSSQLQD--------------TQELLQEETRQKLNVSTKLRQLEEERNSLQDQLDEE 1355
Query: 391 MQKSQNFAEALSAEKLRISXXXXXXXXXXXXXXDSQKKMQKSGIRVKNLDSKVSRIAENV 570
M+ QN +S +++S DS+KK+Q V+ L+ R + +
Sbjct: 1356 MEAKQNLERHISTLNIQLS--------------DSKKKLQDFASTVEALEEGKKRFQKEI 1401
>sp|Q15696|U2AFM_HUMAN U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 2 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 2)
Length = 482
Score = 34.3 bits (77), Expect = 0.29
Identities = 17/46 (36%), Positives = 28/46 (60%)
Frame = +1
Query: 76 ENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAEDKI 213
E E+++ + + +E+ EEQKR +EK QKML QAE+++
Sbjct: 102 EQERKLKEQWEEQQRKEREEEEQKRQEKKEKEEALQKMLDQAENEL 147
>sp|Q15695|U2AFL_HUMAN U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 1 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 1)
Length = 479
Score = 32.7 bits (73), Expect = 0.84
Identities = 16/46 (34%), Positives = 27/46 (58%)
Frame = +1
Query: 76 ENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAEDKI 213
E E+++ + + +E+ EEQK+ +EK QKML QAE+ +
Sbjct: 107 EQERKLKEQWKEQQRKEREEEEQKQQEKKEKEEAVQKMLDQAENDL 152
>sp|Q62377|U2AFM_MOUSE U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 2 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 2)
Length = 462
Score = 32.3 bits (72), Expect = 1.1
Identities = 17/46 (36%), Positives = 27/46 (58%)
Frame = +1
Query: 76 ENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAEDKI 213
E E+++ E + +E+ EEQKR E+ QKML QAE+++
Sbjct: 106 ELERKLKAEWEEQQRKEREEEEQKRQEKREREEAVQKMLDQAENEL 151
>sp|Q64707|U2AFL_MOUSE U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 1 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 1) (SP2)
Length = 428
Score = 31.2 bits (69), Expect = 2.4
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 76 ENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAEDK 210
E E+QI E + +++ EEQK E+ QKML QAE++
Sbjct: 98 EQERQIKAEWEEQQKKQREEEEQKLQEKREREEAVQKMLDQAENE 142
>sp|P21192|ACE2_YEAST Metallothionein expression activator
Length = 770
Score = 30.8 bits (68), Expect = 3.2
Identities = 16/44 (36%), Positives = 30/44 (68%), Gaps = 1/44 (2%)
Frame = +1
Query: 73 IENEKQILNEQLQLTIEEKSIEEQK-RTYLEEKHSEAQKMLSQA 201
+E +K++ NE+L+ + + I+++K R LEE+ AQK++S A
Sbjct: 202 LEKQKEV-NEKLEKQLRDNQIQQEKLRKVLEEQEEVAQKLVSGA 244
>sp|O44952|LONM_CAEEL Lon protease homolog, mitochondrial precursor
Length = 971
Score = 30.4 bits (67), Expect = 4.2
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +1
Query: 79 NEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAE 204
+ K +LNEQL++ +E IE+ ++T + EK E K L+ E
Sbjct: 380 HRKYLLNEQLKVIKKELGIEKDEKTTIIEKIDERIKTLAVPE 421
>sp|Q8MIT6|ROCK1_BOVIN Rho-associated protein kinase 1 (Rho-associated, coiled-coil
containing protein kinase 1) (p160 ROCK-1) (p160ROCK)
Length = 441
Score = 30.4 bits (67), Expect = 4.2
Identities = 29/124 (23%), Positives = 54/124 (43%)
Frame = +1
Query: 73 IENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQKMLSQAEDKITSXXXXXXXXXXX 252
+++EK+ L QL L + E+ R LEE++ E L+Q K S
Sbjct: 288 LQSEKETLATQLDLAETKAESEQLARGLLEEQYFE----LTQESKKAAS----------- 332
Query: 253 XXXXXXXXXXXXTIRNATMALLEEEQLAKVQLEKLLEQQNDTLHTEMQKSQNFAEALSAE 432
T ++ T++ LEE + +LL ++N+ L +M+K++ + E
Sbjct: 333 ------RNRQEITDKDHTLSRLEETNSMLTKDIELLRKENEELTDKMRKAEEEYKLKKEE 386
Query: 433 KLRI 444
++ I
Sbjct: 387 EINI 390
>sp|Q9PLL9|TIG_CHLMU Trigger factor (TF)
Length = 433
Score = 30.4 bits (67), Expect = 4.2
Identities = 14/38 (36%), Positives = 25/38 (65%)
Frame = +1
Query: 73 IENEKQILNEQLQLTIEEKSIEEQKRTYLEEKHSEAQK 186
I + +++LN +L + +EEQK+T LEE ++A+K
Sbjct: 315 ILSREKLLNARLVKYCSDSELEEQKQTLLEEAKADARK 352
>sp|P39922|MYS3_HYDAT Myosin heavy chain, clone 203
Length = 539
Score = 30.0 bits (66), Expect = 5.4
Identities = 13/46 (28%), Positives = 28/46 (60%)
Frame = +1
Query: 298 NATMALLEEEQLAKVQLEKLLEQQNDTLHTEMQKSQNFAEALSAEK 435
NAT+A ++EE A + + LE++ L+ E+ +++N + + +K
Sbjct: 400 NATIAEIQEENEAMKKAKAKLEKEKSALNNELNETKNSLDQIKKQK 445
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.307 0.123 0.305
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,465,127
Number of Sequences: 369166
Number of extensions: 679522
Number of successful extensions: 2370
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2356
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5365037700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)