Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_D17
(879 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Int... 64 6e-10
sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6 64 7e-10
sp|P68907|PZRN3_RAT PDZ domain-containing RING finger prote... 55 2e-07
sp|Q69ZS0|PZRN3_MOUSE PDZ domain-containing RING finger pro... 55 3e-07
sp|Q9UPQ7|PZRN3_HUMAN PDZ domain-containing RING finger pro... 53 1e-06
sp|Q61382|TRAF4_MOUSE TNF receptor-associated factor 4 (Cys... 52 2e-06
sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cys... 51 4e-06
sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RIN... 51 4e-06
sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5 49 1e-05
sp|P39429|TRAF2_MOUSE TNF receptor-associated factor 2 47 7e-05
>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
(RING finger protein 85)
Length = 522
Score = 63.9 bits (154), Expect = 6e-10
Identities = 35/119 (29%), Positives = 55/119 (46%)
Frame = +2
Query: 53 EIMKKFDFHIENQFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNTD 232
E ++ +D + ES + C +C L A +PC RFC CI + CP + +
Sbjct: 51 EEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNE 110
Query: 233 DCKIELLSLNENVVIDHPTNNKILRLKVKCPENACNFKDELKNIENHIRICNYRPVCCP 409
+ L + D+ +IL L VKCP C K EL+++E+H C + + CP
Sbjct: 111 ------ILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCP 163
>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
Length = 530
Score = 63.5 bits (153), Expect = 7e-10
Identities = 36/119 (30%), Positives = 55/119 (46%)
Frame = +2
Query: 53 EIMKKFDFHIENQFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNTD 232
E ++ +D + ES + C +C L A +PC RFC CI + CP + +
Sbjct: 51 EEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNE 110
Query: 233 DCKIELLSLNENVVIDHPTNNKILRLKVKCPENACNFKDELKNIENHIRICNYRPVCCP 409
+ L + D+ +IL L VKCP C K EL+++E+H C + V CP
Sbjct: 111 ------ILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCP 163
>sp|P68907|PZRN3_RAT PDZ domain-containing RING finger protein 3 (Semaphorin cytoplasmic
domain-associated protein 3) (SEMACAP3 protein)
Length = 1062
Score = 55.5 bits (132), Expect = 2e-07
Identities = 34/116 (29%), Positives = 55/116 (47%), Gaps = 2/116 (1%)
Frame = +2
Query: 83 ENQFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNTDDCKIELLSLN 262
+ + + D C LC K+L + +PC FC C+ ++ QE SCP C+ L +
Sbjct: 9 DGEVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQE-GSCPSR---CRGRLSAKE 64
Query: 263 ENVVIDHPTNNKILRLKVKCPENA--CNFKDELKNIENHIRICNYRPVCCPFLNIG 424
N V+ P IL+L +KC A C +L+++ H+ C++ P C G
Sbjct: 65 LNHVL--PLKRLILKLDIKCAHAARGCGRVVKLQDLPEHLERCDFAPARCRHAGCG 118
>sp|Q69ZS0|PZRN3_MOUSE PDZ domain-containing RING finger protein 3 (Semaphorin cytoplasmic
domain-associated protein 3) (SEMACAP3 protein)
Length = 1063
Score = 54.7 bits (130), Expect = 3e-07
Identities = 34/116 (29%), Positives = 54/116 (46%), Gaps = 2/116 (1%)
Frame = +2
Query: 83 ENQFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNTDDCKIELLSLN 262
+ + D C LC K+L + +PC FC C+ ++ QE SCP C+ L +
Sbjct: 9 DGDVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQE-GSCPAR---CRGRLSAKE 64
Query: 263 ENVVIDHPTNNKILRLKVKCPENA--CNFKDELKNIENHIRICNYRPVCCPFLNIG 424
N V+ P IL+L +KC A C +L+++ H+ C++ P C G
Sbjct: 65 LNHVL--PLKRLILKLDIKCAHAARGCGRVVKLQDLPEHLERCDFAPARCRHAGCG 118
>sp|Q9UPQ7|PZRN3_HUMAN PDZ domain-containing RING finger protein 3 (Ligand of Numb-protein
X 3) (Semaphorin cytoplasmic domain-associated protein
3) (SEMACAP3 protein)
Length = 1066
Score = 53.1 bits (126), Expect = 1e-06
Identities = 33/116 (28%), Positives = 52/116 (44%), Gaps = 2/116 (1%)
Frame = +2
Query: 83 ENQFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNTDDCKIELLSLN 262
+ + D C LC K+L + +PC FC C+ ++ QE SCP C+ L +
Sbjct: 9 DGDVDPDLKCALCHKVLEDPLTTPCGHVFCAGCVLPWVVQE-GSCPAR---CRGRLSAKE 64
Query: 263 ENVVIDHPTNNKILRLKVKC--PENACNFKDELKNIENHIRICNYRPVCCPFLNIG 424
N V+ P IL+L +KC C +L+ + H+ C++ P C G
Sbjct: 65 LNHVL--PLKRLILKLDIKCAYATRGCGRVVKLQQLPEHLERCDFAPARCRHAGCG 118
>sp|Q61382|TRAF4_MOUSE TNF receptor-associated factor 4 (Cysteine-rich motif associated to
RING and Traf domains protein 1)
Length = 470
Score = 52.0 bits (123), Expect = 2e-06
Identities = 29/120 (24%), Positives = 57/120 (47%), Gaps = 3/120 (2%)
Frame = +2
Query: 59 MKKFDFHIENQFESDFLCVLCKKLLINAY-ISPCECRFCFNCISDYLNQEKKSCPGNTDD 235
M D+ + + LC LC K + ++ +S C R+C NC+ ++L++ CP D
Sbjct: 1 MPGLDYKFPEKPKRRLLCPLCGKPMRDSVEVSTCGHRYCDNCLQEFLSEGVFKCP--EDQ 58
Query: 236 CKIELLSLNENVVIDHPTNNKILRLKVKC--PENACNFKDELKNIENHIRICNYRPVCCP 409
++ + D ++L L ++C E C + L++++ H+ C++ V CP
Sbjct: 59 LPLDY----AKIYPDPELEVQVLGLAIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVVPCP 114
>sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cysteine-rich domain associated
with RING and Traf domains protein 1) (Malignant 62)
(RING finger protein 83)
Length = 470
Score = 51.2 bits (121), Expect = 4e-06
Identities = 29/120 (24%), Positives = 55/120 (45%), Gaps = 3/120 (2%)
Frame = +2
Query: 59 MKKFDFHIENQFESDFLCVLCKKLLIN-AYISPCECRFCFNCISDYLNQEKKSCPGNTDD 235
M FD+ + + LC LC K + +S C RFC C+ ++L++ CP D
Sbjct: 1 MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCP--EDQ 58
Query: 236 CKIELLSLNENVVIDHPTNNKILRLKVKC--PENACNFKDELKNIENHIRICNYRPVCCP 409
++ + D ++L L ++C E C + L++++ H+ C++ + CP
Sbjct: 59 LPLDYAKIYP----DPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCP 114
Score = 31.2 bits (69), Expect = 4.0
Identities = 27/138 (19%), Positives = 50/138 (36%), Gaps = 1/138 (0%)
Frame = +2
Query: 155 CECRFCFNCISDYLNQEKKSCPGNTDDCKIELLSLNENVVIDHPTNNKILRLKVKCPENA 334
C R ++ + E CP T C + + H + RL V CP
Sbjct: 171 CGARMMRRLLAQHATSE---CPKRTQPCTYCTKEFVFDTIQSH--QYQCPRLPVACPNQC 225
Query: 335 CNFKDELKNIENHIR-ICNYRPVCCPFLNIGCNSSNIHAGNVNDHLQLEIGNHTKLLIDC 511
+++ H++ CN V CPF + GC H++ + H ++
Sbjct: 226 GVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMAR-HVEESVKPHLAMMCAL 284
Query: 512 IDNLNNEIGRLKVSVDKL 565
+ E+ L+ +++L
Sbjct: 285 VSRQRQELQELRRELEEL 302
>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
Length = 557
Score = 51.2 bits (121), Expect = 4e-06
Identities = 62/285 (21%), Positives = 110/285 (38%), Gaps = 28/285 (9%)
Frame = +2
Query: 68 FDFHIENQF----ESDFLCVLCKKLLINAYISPCECRFCFNCISDYLN-QEKKSCPGNTD 232
F+ IE QF E + C C +L N + + C RFC +CI CP + +
Sbjct: 27 FEPSIEYQFVERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNTVPICPVDKE 86
Query: 233 DCKIELLSLNENVVIDHPTNNKILRLKVKCPE-NACNFKDELKNIENHIRICNYRPVCCP 409
K ++ V D+ ++L L V C CN K L ++H++ C ++PV C
Sbjct: 87 VIK------SQEVFKDNCCKREVLNLYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQCS 140
Query: 410 FLNIGCNSSNIHAGNVNDHLQLEIGNHTKLLIDC-----IDNLNNEIGRLKVSVDKLGEE 574
N C + ++ +HL + + C + NL N L
Sbjct: 141 --NEKCREPVLRK-DLKEHLSASCQFRKEKCLYCKKDVVVINLQNHEENLCPEYPVFCPN 197
Query: 575 N---KMLKNEINEIKLDSEKKQMKC--------------DEQDREIVLLNEDMVALRNVD 703
N +LK E++E + + C + Q E L E M + +
Sbjct: 198 NCAKIILKTEVDEHLAVCPEAEQDCPFKHYGCAVTDKRRNLQQHEHSALREHMRLVLEKN 257
Query: 704 QYLRDDFNELKLGGERKQREIDKLNKKLQECNQNLNRIKSILKKN 838
L + ++L E+K+ +I +L + +++ + + + KN
Sbjct: 258 VQLEEQISDLHKSLEQKESKIQQLAETIKKLEKEFKQFAQLFGKN 302
>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
Length = 558
Score = 49.3 bits (116), Expect = 1e-05
Identities = 37/136 (27%), Positives = 64/136 (47%), Gaps = 3/136 (2%)
Frame = +2
Query: 71 DFHIENQFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNTDDCKIEL 250
++ Q E + C C +L N + + C RFC CI +E S P C ++
Sbjct: 32 EYQFVEQLEERYKCAFCHSVLHNPHQTGCGHRFCQQCIRSL--RELNSVP----ICPVDK 85
Query: 251 LSLN-ENVVIDHPTNNKILRLKVKCPENA--CNFKDELKNIENHIRICNYRPVCCPFLNI 421
+ + V D+ ++L L V C +NA CN + L ++H++ C+++ V CP N
Sbjct: 86 EVIKPQEVFKDNCCKREVLNLHVYC-KNAPGCNARIILGRFQDHLQHCSFQAVPCP--NE 142
Query: 422 GCNSSNIHAGNVNDHL 469
C + + +V +HL
Sbjct: 143 SCREAMLRK-DVKEHL 157
Score = 37.7 bits (86), Expect = 0.042
Identities = 33/132 (25%), Positives = 58/132 (43%)
Frame = +2
Query: 236 CKIELLSLNENVVIDHPTNNKILRLKVKCPENACNFKDELKNIENHIRICNYRPVCCPFL 415
CK +++ N + DH N+ V CP N C + H+ +C CPF
Sbjct: 171 CKRDIVVTN---LQDHEENS-CPAYPVSCP-NRCVQTIPRARVNEHLTVCPEAEQDCPFK 225
Query: 416 NIGCNSSNIHAGNVNDHLQLEIGNHTKLLIDCIDNLNNEIGRLKVSVDKLGEENKMLKNE 595
+ GC GN+ +H + + +H L+++ L I L S+++ +E+K+ +
Sbjct: 226 HYGCTVKG-KRGNLLEHERAALQDHMLLVLEKNYQLEQRISDLYQSLEQ--KESKIQQLA 282
Query: 596 INEIKLDSEKKQ 631
K + E KQ
Sbjct: 283 ETVKKFEKELKQ 294
>sp|P39429|TRAF2_MOUSE TNF receptor-associated factor 2
Length = 501
Score = 47.0 bits (110), Expect = 7e-05
Identities = 28/111 (25%), Positives = 52/111 (46%), Gaps = 4/111 (3%)
Frame = +2
Query: 89 QFESDFLCVLCKKLLINAYISPCECRFCFNCISDYLNQEKKSCPGNT-DDCKIELLSLNE 265
+ E+ +LC CK +L + + C R+C C++ L+ ++C + E +S+ E
Sbjct: 27 RLEAKYLCSACKNILRRPFQAQCGHRYCSFCLTSILSSGPQNCAACVYEGLYEEGISILE 86
Query: 266 --NVVIDHPTNNKILRLKVKCPENACNFKDELKNIEN-HIRICNYRPVCCP 409
+ D+ ++ L CP + C +K LK E+ H +C + CP
Sbjct: 87 SSSAFPDNAARREVESLPAVCPNDGCTWKGTLKEYESCHEGLCPFLLTECP 137
Score = 33.9 bits (76), Expect = 0.61
Identities = 20/81 (24%), Positives = 36/81 (44%)
Frame = +2
Query: 356 KNIENHIRICNYRPVCCPFLNIGCNSSNIHAGNVNDHLQLEIGNHTKLLIDCIDNLNNEI 535
+ ++H+R C+ V C F +GC S + N+ DH + H LL+
Sbjct: 200 ETFQDHVRACSKCRVLCRFHTVGC-SEMVETENLQDHELQRLREHLALLLSSFLEAQASP 258
Query: 536 GRLKVSVDKLGEENKMLKNEI 598
G L +L + ++L+ +I
Sbjct: 259 GTLNQVGPELLQRCQILEQKI 279
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,040,642
Number of Sequences: 369166
Number of extensions: 1690614
Number of successful extensions: 6553
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5954
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6483
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8742211660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)