Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_D14
(801 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--pr... 289 5e-78
sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--pr... 288 2e-77
sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--pr... 286 5e-77
sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--pr... 286 6e-77
sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--pr... 266 4e-71
sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosacch... 246 4e-65
sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--pro... 93 1e-18
sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synt... 35 0.31
sp|O78432|YCF30_GUITH Putative HTH-type transcriptional reg... 33 0.91
sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc... 31 4.5
>sp|Q05052|OST48_CANFA Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit)
Length = 445
Score = 289 bits (740), Expect = 5e-78
Identities = 146/246 (59%), Positives = 188/246 (76%), Gaps = 1/246 (0%)
Frame = +1
Query: 1 LLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASV 180
+L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V
Sbjct: 203 ILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFNSAV 262
Query: 181 EHAI-SKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVH 357
+ A Q ++++GN LA A+S+WVFKE GVLRVG VSH ++ P YT+ + V
Sbjct: 263 QKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVE 321
Query: 358 YEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFL 537
Y IVI++L ++G W PF DD+QLEF RIDPFVR + +K GK+ + FKLPDVYGVF+F
Sbjct: 322 YSIVIEQL-SNGRWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFK 380
Query: 538 VDYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKD 717
VDY+R+GYTH+HSSTQVSVRPL HTQYERFI +AYPYYASA SMM+G+ +FS +FL+ K
Sbjct: 381 VDYNRLGYTHLHSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMLGLFIFSTVFLHMK- 439
Query: 718 DKEKNE 735
+KEK++
Sbjct: 440 EKEKSD 445
>sp|O54734|OST48_MOUSE Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 441
Score = 288 bits (736), Expect = 2e-77
Identities = 146/246 (59%), Positives = 186/246 (75%), Gaps = 1/246 (0%)
Frame = +1
Query: 1 LLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASV 180
+L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V
Sbjct: 199 ILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAV 258
Query: 181 EHAI-SKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVH 357
+ A Q ++++GN LA A+S+WVFKE GVLRVG VSH + P YT+ + V
Sbjct: 259 QKATPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGEMAP-PNAYTVTDLVE 317
Query: 358 YEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFL 537
Y IVI++L ++G W PF DD+QLEF RIDPFVR + RK GK+ + FKLPDVYGVF+F
Sbjct: 318 YSIVIEQL-SNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFK 376
Query: 538 VDYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKD 717
VDY+R+GYTH++SSTQVSVRPL HTQYERFI +AYPYYASA SMM G+ +FS +FL+ K
Sbjct: 377 VDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMK- 435
Query: 718 DKEKNE 735
+KEK++
Sbjct: 436 EKEKSD 441
>sp|P39656|OST48_HUMAN Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit)
Length = 439
Score = 286 bits (732), Expect = 5e-77
Identities = 144/246 (58%), Positives = 186/246 (75%), Gaps = 1/246 (0%)
Frame = +1
Query: 1 LLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASV 180
+L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F ++V
Sbjct: 197 ILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAV 256
Query: 181 EHAI-SKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVH 357
+ A Q ++++GN LA A+S+WVFKE GVLRVG VSH ++ P YT+ + V
Sbjct: 257 QKAAPGSQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGETAP-PNAYTVTDLVE 315
Query: 358 YEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFL 537
Y IVI +L ++G W PF DD+QLEF RIDPFVR + +K GK+ + FKLPDVYGVF+F
Sbjct: 316 YSIVIQQL-SNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGVFQFK 374
Query: 538 VDYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKD 717
VDY+R+GYTH++SSTQVSVRPL HTQYERFI +AYPYYASA MM+G+ +FS +FL+ K
Sbjct: 375 VDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFPMMLGLFIFSIVFLHMK- 433
Query: 718 DKEKNE 735
+KEK++
Sbjct: 434 EKEKSD 439
>sp|P48440|OST48_CHICK Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit (Oligosaccharyl transferase 48 kDa subunit)
(OST 50 kDa subunit) (DDOST 48 kDa subunit)
Length = 413
Score = 286 bits (731), Expect = 6e-77
Identities = 145/246 (58%), Positives = 187/246 (76%), Gaps = 1/246 (0%)
Frame = +1
Query: 1 LLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASV 180
+L SS++YSF PDKP+ YP++VG NT LI LQARNNARV+F GSLD FSD F+++V
Sbjct: 171 ILTGSSTSYSFFPDKPITQYPHAVGKNTLLIAGLQARNNARVVFSGSLDFFSDAFFSSAV 230
Query: 181 EHAI-SKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVH 357
+ A + ++++GN LA A+S+WVFKE GVLRVG VSH + + P YT+ + V
Sbjct: 231 QKAAPGSKRYSQTGNYELAVALSRWVFKEEGVLRVGAVSHHRVGE-LAPPNAYTVTDLVE 289
Query: 358 YEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFL 537
Y IVI++L +DG W PF DD+QLEF RIDPFVR + R GK+ + FKLPDVYGVF+F
Sbjct: 290 YSIVIEKL-SDGKWIPFDGDDIQLEFVRIDPFVRTFLKRNGGKYSVQFKLPDVYGVFQFK 348
Query: 538 VDYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKD 717
VDY+R+GYTH++SSTQVSVRPL HTQYERFI +AYPYYA A SMMVG+ +FS +FL+ K
Sbjct: 349 VDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYAGAFSMMVGLFMFSIVFLHMK- 407
Query: 718 DKEKNE 735
+KEK++
Sbjct: 408 EKEKSD 413
>sp|Q24319|OST48_DROME Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48
kDa subunit precursor (Oligosaccharyl transferase 48 kDa
subunit) (DDOST 48 kDa subunit) (OST5OP) (DrOST)
(DmOST50)
Length = 449
Score = 266 bits (681), Expect = 4e-71
Identities = 138/241 (57%), Positives = 175/241 (72%), Gaps = 1/241 (0%)
Frame = +1
Query: 1 LLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASV 180
LL A S+AYS+NP+ V DYP++VG T LI ALQARNNARV+F GSL FSD+ FT +V
Sbjct: 197 LLTAESTAYSYNPEASVSDYPHAVGRGTLLIAALQARNNARVVFSGSLLFFSDESFTTAV 256
Query: 181 EHAISKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHY 360
++A S H +GN +A +ISKWVF ETG LRV +V H + + + YTI + V Y
Sbjct: 257 QYAQSGVFHKLAGNRDVAESISKWVFGETGRLRVASVQHHKEGELLPPDQAYTITDPVVY 316
Query: 361 EIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKE-GKFYLDFKLPDVYGVFKFL 537
I I+EL G W F A D+QLEF RIDPFVR + + G + FK+PDVYGV++F
Sbjct: 317 TIGIEEL-VQGEWRAFKASDIQLEFVRIDPFVRTYLKQTNTGAYQAKFKIPDVYGVYQFK 375
Query: 538 VDYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKD 717
VDY+RVGYTH++S+TQVSVRPL HTQYERFI +A+PYY SA SMM+GV +FSF+FL+FKD
Sbjct: 376 VDYNRVGYTHLYSTTQVSVRPLEHTQYERFIPSAFPYYTSAFSMMIGVFVFSFVFLHFKD 435
Query: 718 D 720
+
Sbjct: 436 E 436
>sp|P45971|OST48_CAEEL Probable dolichyl-diphosphooligosaccharide--protein
glycosyltransferase 48 kDa subunit precursor
(Oligosaccharyl transferase 48 kDa subunit)
Length = 445
Score = 246 bits (629), Expect = 4e-65
Identities = 127/243 (52%), Positives = 163/243 (67%)
Frame = +1
Query: 1 LLHASSSAYSFNPDKPVDDYPNSVGINTQLITALQARNNARVLFVGSLDLFSDDLFTASV 180
++ AS +AYS++P P+ G T L+ LQ+RNNAR++F GS +LFS+ F+A
Sbjct: 192 IVRASGTAYSYDPKAVRATNPSIAGSRTLLVGGLQSRNNARIVFTGSSELFSNTFFSAKT 251
Query: 181 EHAISKQTHARSGNEALATAISKWVFKETGVLRVGNVSHSLADKSVENpreYTIMENVHY 360
A+SGN ATAI++WV KE+GVLRV V+H ++V Y I E+V Y
Sbjct: 252 NSVNPSVQGAQSGNADFATAITRWVMKESGVLRVKTVNHHKKGETVPPVEGYFITEDVVY 311
Query: 361 EIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLV 540
I I+EL+ +G W PF DVQLEF RIDPFVR + G+ + FKLPDV GVFKFLV
Sbjct: 312 TIEIEELK-NGKWVPFQGKDVQLEFVRIDPFVRATLKNSNGRLSVAFKLPDVLGVFKFLV 370
Query: 541 DYHRVGYTHVHSSTQVSVRPLMHTQYERFIQAAYPYYASAISMMVGVILFSFLFLYFKDD 720
DY RVGYTH++ QVSVRPL HTQYERFI++AYPYYAS+ SMM G++LFS ++LY KD
Sbjct: 371 DYRRVGYTHLYDVQQVSVRPLWHTQYERFIRSAYPYYASSFSMMAGLVLFSIVYLYHKDT 430
Query: 721 KEK 729
K
Sbjct: 431 PVK 433
>sp|P33767|OSTB_YEAST Dolichyl-diphosphooligosaccharide--protein glycosyltransferase beta
subunit precursor (Oligosaccharyl transferase beta
subunit)
Length = 430
Score = 92.8 bits (229), Expect = 1e-18
Identities = 60/214 (28%), Positives = 100/214 (46%), Gaps = 8/214 (3%)
Frame = +1
Query: 88 LITALQARNNARVLFVGSLDLFSDDLFTASVEHAISKQTHARSGNEALATAISKWVFKET 267
L+ Q NNAR++++GS D + + N+ A + KW F E
Sbjct: 217 LVVGFQNLNNARLVWIGSSDFLKNK---------------NQDSNQEFAKELLKWTFNEK 261
Query: 268 GVLRVGNVSHSLADKSVENpreYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRID 447
V++ + HS AD + + Y I + V Y + E + W P IADD+Q E ++D
Sbjct: 262 SVIKSVHAVHSHADGTSYDEEPYKIKDKVIYSVGFSEWNGE-EWLPHIADDIQFELRQVD 320
Query: 448 PFVRQNMLRK----EGKFYL--DFKLPDVYGVFKFLVDYHRVGYTHVHSSTQVSVRPLMH 609
P+ R + E ++Y +F LPD +GVF FL DY ++G + ++R L +
Sbjct: 321 PYYRLTLSPSGNDSETQYYTTGEFILPDRHGVFTFLTDYRKIGLSFTTDKDVKAIRHLAN 380
Query: 610 TQYERF--IQAAYPYYASAISMMVGVILFSFLFL 705
+Y R I ++ Y ++ ++V I F F+
Sbjct: 381 DEYPRSWEISNSWVYISAICGVIVAWIFFVVSFV 414
>sp|O26270|PURQ_METTH Phosphoribosylformylglycinamidine synthase I (FGAM synthase I)
Length = 218
Score = 34.7 bits (78), Expect = 0.31
Identities = 33/135 (24%), Positives = 60/135 (44%), Gaps = 4/135 (2%)
Frame = +1
Query: 157 DDLFTASVEHAISKQTHARSGNEALAT----AISKWVFKETGVLRVGNVSHSLADKSVEN 324
D L + S + R+G A T A+ + V +E VL + N + LA+ +
Sbjct: 39 DHLDAVVIPGGFSYGDYLRAGAIAAITPVMDAVRELVREEKPVLGICNGAQILAEVGLV- 97
Query: 325 preYTIMENVHYEIVIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFK 504
P +T+ E+ + ELR T TPF ++ D +R + EG++Y D
Sbjct: 98 PGVFTVNEHPKFNCQWTELRVKTTRTPFTG------LFKKDEVIRMPVAHAEGRYYHD-N 150
Query: 505 LPDVYGVFKFLVDYH 549
+ +V+ + ++ +H
Sbjct: 151 ISEVWENDQVVLQFH 165
>sp|O78432|YCF30_GUITH Putative HTH-type transcriptional regulator ycf30
Length = 310
Score = 33.1 bits (74), Expect = 0.91
Identities = 17/59 (28%), Positives = 32/59 (54%)
Frame = +1
Query: 367 VIDELRADGTWTPFIADDVQLEFYRIDPFVRQNMLRKEGKFYLDFKLPDVYGVFKFLVD 543
V DELR+ TP++ D++ L ++ PF + +++KE + L F D + ++D
Sbjct: 155 VPDELRSKLQITPYVDDELALILPKLHPFSKLEVIQKEDLYRLRFITLDKQSTIRRVID 213
>sp|P34374|UNC36_CAEEL Voltage-dependent calcium channel unc-36 precursor (Uncoordinated
protein 36)
Length = 1249
Score = 30.8 bits (68), Expect = 4.5
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 2/33 (6%)
Frame = -1
Query: 423 DIISNERSPCSISPQLVNNDLVMH--ILHYCVF 331
D NER PCS SP +V+ ++ LH+C+F
Sbjct: 1217 DDSENERRPCSTSPTIVSIFQILFGVFLHFCIF 1249
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,154,619
Number of Sequences: 369166
Number of extensions: 1795407
Number of successful extensions: 5258
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5068
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5236
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)