Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_C21
(507 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8LAX0|HMT3_ARATH Homocysteine S-methyltransferase 3 (S-... 100 3e-21
sp|P56707|SMTA_ASTBI Selenocysteine methyltransferase (SECY... 90 3e-18
sp|Q9FUN0|HMT1_MAIZE Homocysteine S-methyltransferase 1 (S-... 85 9e-17
sp|Q9FUM9|HMT2_MAIZE Homocysteine S-methyltransferase 2 (S-... 85 1e-16
sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-... 84 2e-16
sp|Q9FUM8|HMT3_MAIZE Homocysteine S-methyltransferase 3 (S-... 84 2e-16
sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-me... 82 9e-16
sp|Q9M1W4|HMT2_ARATH Homocysteine S-methyltransferase 2 (S-... 80 3e-15
sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-... 76 5e-14
sp|Q12525|MHT1_YEAST Homocysteine S-methyltransferase 1 (S-... 58 1e-08
>sp|Q8LAX0|HMT3_ARATH Homocysteine S-methyltransferase 3 (S-methylmethionine:homocysteine
methyltransferase 3) (SMM:Hcy S-methyltransferase 3)
(AtHMT-3)
Length = 347
Score = 100 bits (248), Expect = 3e-21
Identities = 54/153 (35%), Positives = 82/153 (53%), Gaps = 1/153 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
+AFET+P L+ E DL+ E + AW S TS DG+ G+ + + + + S KN
Sbjct: 186 IAFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVAD--SCKN 243
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
V+ G+NC P + I R K IV+YPN+GEV + LN+ W EG +E F
Sbjct: 244 VVAIGINCTAPRYI---HALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDF 300
Query: 360 YEYIPSWIEAGAEWIGGCCRMSADRMIRVSDII 458
Y+ W +AGA GGCCR + + + ++ ++
Sbjct: 301 VSYVSKWRDAGASLFGGCCRTTPNTIRAIAKVL 333
>sp|P56707|SMTA_ASTBI Selenocysteine methyltransferase (SECYS-methyltransferase)
(SECYS-MT)
Length = 338
Score = 89.7 bits (221), Expect = 3e-18
Identities = 50/155 (32%), Positives = 80/155 (51%), Gaps = 1/155 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYPN-TSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
LAFE +P L+ + DL+ E T AW + TS DG + G+ +++ S
Sbjct: 180 LAFEAVPNKLEAQAYADLLEEENIITPAWFAFTSKDGNNVVSGDSIEECGSIAE--SCDK 237
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
V+ G+NC P + D + ++ K IV+YPN+GE +++ + W GVT+ F
Sbjct: 238 VVAVGINCTPPRFI-HDLILLLKKVTA--KPIVIYPNSGETYDAIRKEWGQNSGVTDEDF 294
Query: 360 YEYIPSWIEAGAEWIGGCCRMSADRMIRVSDIIRS 464
Y+ W E+GA +GGCCR + D + + I+ S
Sbjct: 295 VSYVDKWCESGASLVGGCCRTTPDTIRGIYKILSS 329
>sp|Q9FUN0|HMT1_MAIZE Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(ZmHMT-1)
Length = 323
Score = 85.1 bits (209), Expect = 9e-17
Identities = 47/154 (30%), Positives = 82/154 (53%), Gaps = 1/154 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
+AFE +P ++ + +++L+ E +WI +S+DG + GE D ++ +N +++
Sbjct: 170 IAFEAIPNQMEAQALVELLEEEKVQIPSWICFSSVDGKNLCSGESFADCLKILN--ASEK 227
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
V G+NC P ++ +C+ R + K I +YPN+GEV + + W E + +F
Sbjct: 228 VAVVGVNCTPP-QFIEGIICEFRKQTK--KAIAVYPNSGEVWDGRAKRWLPVECLGHKSF 284
Query: 360 YEYIPSWIEAGAEWIGGCCRMSADRMIRVSDIIR 461
W EAGA IGGCCR + + VS I++
Sbjct: 285 DALAKRWQEAGASLIGGCCRTTPSTIRAVSKILK 318
>sp|Q9FUM9|HMT2_MAIZE Homocysteine S-methyltransferase 2 (S-methylmethionine:homocysteine
methyltransferase 2) (SMM:Hcy S-methyltransferase 2)
(ZmHMT-2)
Length = 339
Score = 84.7 bits (208), Expect = 1e-16
Identities = 47/140 (33%), Positives = 72/140 (51%), Gaps = 1/140 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
+AFET+P L+ E ++L+ E N AW S S DG+H G+ L + ++ +
Sbjct: 179 IAFETIPNKLEAEAYVELLEECNINIPAWFSFNSKDGVHIVSGDSLIECTTIADKCAKVG 238
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
+G +NC P + + IR DK I++YPN+GE + + W GV++ F
Sbjct: 239 AVG--INCTPPRFI-HGLILSIRKVT--DKPILIYPNSGERYDGEKKEWVESTGVSDGDF 293
Query: 360 YEYIPSWIEAGAEWIGGCCR 419
Y+ W + GA IGGCCR
Sbjct: 294 VSYVNEWCKDGAVLIGGCCR 313
>sp|Q9SDL7|HMT1_ARATH Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
(AtHMT-1)
Length = 326
Score = 84.0 bits (206), Expect = 2e-16
Identities = 47/150 (31%), Positives = 81/150 (54%), Gaps = 1/150 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
LAFET+P L+ + ++L+ E AWI TS+DG GE ++ +E +N+ + N
Sbjct: 176 LAFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNK--SNN 233
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
+ G+NC P + + + + ++L K IV+YPN+GEV + + W + ++ F
Sbjct: 234 IYAVGINCAPPQFI--ENLIRKFAKLTK-KAIVVYPNSGEVWDGKAKQWLPSQCFGDDEF 290
Query: 360 YEYIPSWIEAGAEWIGGCCRMSADRMIRVS 449
+ W + GA+ IGGCCR + + +S
Sbjct: 291 EMFATKWRDLGAKLIGGCCRTTPSTINAIS 320
>sp|Q9FUM8|HMT3_MAIZE Homocysteine S-methyltransferase 3 (S-methylmethionine:homocysteine
methyltransferase 3) (SMM:Hcy S-methyltransferase 3)
(ZmHMT-3)
Length = 338
Score = 84.0 bits (206), Expect = 2e-16
Identities = 45/140 (32%), Positives = 73/140 (52%), Gaps = 1/140 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
+AFET+P L+ + ++L+ E N +W+S S DG+H G+ L + ++ +
Sbjct: 179 IAFETIPNKLEAQAYVELLEECNINIPSWLSFNSKDGVHVVSGDSLIECATIADKCAKVG 238
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
+G +NC P + + IR DK I++YPN+GE + + W GV++ F
Sbjct: 239 AVG--INCTPPRFI-HGLILSIRKVT--DKPILIYPNSGERYDGEKKEWVESTGVSDGDF 293
Query: 360 YEYIPSWIEAGAEWIGGCCR 419
Y+ W + GA IGGCCR
Sbjct: 294 VSYVNEWCKDGAALIGGCCR 313
>sp|Q47690|MMUM_ECOLI Homocysteine S-methyltransferase (S-methylmethionine:homocysteine
methyltransferase)
Length = 310
Score = 81.6 bits (200), Expect = 9e-16
Identities = 45/140 (32%), Positives = 74/140 (52%), Gaps = 1/140 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYPNTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKNV 182
LA ET+P ++E + +L+ YP AW S T D H + G L DV+ + Y V
Sbjct: 165 LACETLPNFSEIEALAELLTAYPRARAWFSFTLRDSEHLSDGTPLRDVVALLAGY--PQV 222
Query: 183 IGFGLNCVIPLMVVKDTVCKIRSRLNPDKI-IVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
+ G+NC+ +++T ++ + +V+YPN+GE +++++ W+ G
Sbjct: 223 VALGINCI----ALENTTAALQHLHGLTVLPLVVYPNSGEHYDAVSKTWHH-HGEHCAQL 277
Query: 360 YEYIPSWIEAGAEWIGGCCR 419
+Y+P W AGA IGGCCR
Sbjct: 278 ADYLPQWQAAGARLIGGCCR 297
>sp|Q9M1W4|HMT2_ARATH Homocysteine S-methyltransferase 2 (S-methylmethionine:homocysteine
methyltransferase 2) (SMM:Hcy S-methyltransferase 2)
(AtHMT-2)
Length = 333
Score = 80.1 bits (196), Expect = 3e-15
Identities = 42/140 (30%), Positives = 72/140 (51%), Gaps = 1/140 (0%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
+AFET+P ++ + DL+ E W S S DG++ G+ + + I + +
Sbjct: 180 IAFETIPNKIEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAE--NCEK 237
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTENTF 359
V+ G+NC P + + V +I + K I++YPN+GE ++ + W GV + F
Sbjct: 238 VVAVGINCTPPRFI-EGLVLEIEKVTS--KPILVYPNSGESYDADRKEWVENTGVGDEDF 294
Query: 360 YEYIPSWIEAGAEWIGGCCR 419
Y+ W++AG +GGCCR
Sbjct: 295 VSYVEKWMDAGVSLLGGCCR 314
>sp|Q9FUM7|HMT4_MAIZE Homocysteine S-methyltransferase 4 (S-methylmethionine:homocysteine
methyltransferase 4) (SMM:Hcy S-methyltransferase 4)
(ZmHMT-4)
Length = 342
Score = 75.9 bits (185), Expect = 5e-14
Identities = 48/155 (30%), Positives = 77/155 (49%), Gaps = 2/155 (1%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYP-NTSAWISMTSLDGIHTAHGELLDDVIEYINQYSTKN 179
+AFET+P L+ ++ +L+ E AW S TS DG++ A G+ +++ + S
Sbjct: 180 IAFETIPNKLEAQVYAELLEENGIRIPAWFSFTSKDGVNAASGDPINECAAVAD--SCPR 237
Query: 180 VIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVT-ENT 356
V G+NC P + + I+ + K IV+YPN+GE + W +G T +
Sbjct: 238 VDAVGVNCTAPRFI-HGLILSIKKVTS--KPIVVYPNSGETYVAETNEWVDSDGATGTDD 294
Query: 357 FYEYIPSWIEAGAEWIGGCCRMSADRMIRVSDIIR 461
F + W AGA IGGCCR S + ++ +R
Sbjct: 295 FVSRVGEWRRAGAALIGGCCRTSPATVRAIARAVR 329
>sp|Q12525|MHT1_YEAST Homocysteine S-methyltransferase 1 (S-methylmethionine:homocysteine
methyltransferase 1) (SMM:Hcy S-methyltransferase 1)
Length = 324
Score = 58.2 bits (139), Expect = 1e-08
Identities = 36/158 (22%), Positives = 75/158 (47%), Gaps = 3/158 (1%)
Frame = +3
Query: 3 LAFETMPAMLDVELIIDLMREYPNTSAWISMTSLDGIHTAHGELLDDV---IEYINQYST 173
+ FET+P +++ I+ + + +I ++ D G L+++ I+ +
Sbjct: 169 IGFETIPNFHELKAILSWDEDIISKPFYIGLSVDDNSLLRDGTTLEEISVHIKGLGNKIN 228
Query: 174 KNVIGFGLNCVIPLMVVKDTVCKIRSRLNPDKIIVLYPNTGEVLNSLNRHWYFPEGVTEN 353
KN++ G+NCV + K+ P +++YPN+GE+ N + W+ P ++
Sbjct: 229 KNLLLMGVNCVS--FNQSALILKMLHEHLPGMPLLVYPNSGEIYNPKEKTWHRPTNKLDD 286
Query: 354 TFYEYIPSWIEAGAEWIGGCCRMSADRMIRVSDIIRSY 467
+ + +++ GA IGGCCR S + ++ + Y
Sbjct: 287 -WETTVKKFVDNGARIIGGCCRTSPKDIAEIASAVDKY 323
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,369,789
Number of Sequences: 369166
Number of extensions: 1161686
Number of successful extensions: 3744
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3669
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3722
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)