Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_B22
(829 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated pr... 264 3e-70
sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated pr... 263 4e-70
sp|P46734|MP2K3_HUMAN Dual specificity mitogen-activated pr... 258 2e-68
sp|O09110|MP2K3_MOUSE Dual specificity mitogen-activated pr... 256 4e-68
sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated pr... 224 2e-58
sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated pr... 222 9e-58
sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated pr... 221 2e-57
sp|Q23977|HEP_DROME Dual specificity mitogen-activated prot... 187 2e-47
sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kina... 184 3e-46
sp|P33886|WIS1_SCHPO Protein kinase wis1 (Protein kinase sty2) 176 5e-44
>sp|P52564|MP2K6_HUMAN Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 264 bits (674), Expect = 3e-70
Identities = 125/232 (53%), Positives = 172/232 (74%), Gaps = 1/232 (0%)
Frame = +2
Query: 128 NDNPVKDLTDHTEVQVDGVKITIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFAVKQIR 307
+ P +DL + + + DL+ I +LG G YG+V++MRH PS + AVK+IR
Sbjct: 26 SSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIR 85
Query: 308 CSMNDKQQKRILNDLNISMRSTQCPYVVISYGAMYRSGDVWICMELMDSSLDKFYKLVSD 487
++N ++QKR+L DL+ISMR+ CP+ V YGA++R GDVWICMELMD+SLDKFYK V D
Sbjct: 86 ATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVID 145
Query: 488 RKSFIPENLLGYMTFCVVSALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGISGNLV 667
+ IPE++LG + +V ALE+LH L VIHRDVKPSNVLIN G VK+CDFGISG LV
Sbjct: 146 KGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLV 205
Query: 668 NSIAET-NVGTESYLAPERINTEQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
+S+A+T + G + Y+APERIN E N GY+++SD+WSLG++++ELAIL++P+
Sbjct: 206 DSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPY 257
>sp|P70236|MP2K6_MOUSE Dual specificity mitogen-activated protein kinase kinase 6 (MAP
kinase kinase 6) (MAPKK 6) (MAPK/ERK kinase 6) (SAPKK3)
Length = 334
Score = 263 bits (672), Expect = 4e-70
Identities = 124/232 (53%), Positives = 172/232 (74%), Gaps = 1/232 (0%)
Frame = +2
Query: 128 NDNPVKDLTDHTEVQVDGVKITIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFAVKQIR 307
+ P +DL + + + DL+ I +LG G YG+V++MRH PS + AVK+IR
Sbjct: 26 SSTPPRDLDSKACISIGNQNFEVKADDLEPIVELGRGAYGVVEKMRHVPSGQIMAVKRIR 85
Query: 308 CSMNDKQQKRILNDLNISMRSTQCPYVVISYGAMYRSGDVWICMELMDSSLDKFYKLVSD 487
++N ++QKR+L DL++SMR+ CP+ V YGA++R GDVWICMELMD+SLDKFYK V D
Sbjct: 86 ATVNSQEQKRLLMDLDVSMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVID 145
Query: 488 RKSFIPENLLGYMTFCVVSALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGISGNLV 667
+ IPE++LG + +V ALE+LH L VIHRDVKPSNVLIN G VK+CDFGISG LV
Sbjct: 146 KGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINTLGQVKMCDFGISGYLV 205
Query: 668 NSIAET-NVGTESYLAPERINTEQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
+S+A+T + G + Y+APERIN E N GY+++SD+WSLG++++ELAIL++P+
Sbjct: 206 DSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPY 257
>sp|P46734|MP2K3_HUMAN Dual specificity mitogen-activated protein kinase kinase 3 (MAP
kinase kinase 3) (MAPKK 3) (MAPK/ERK kinase 3)
Length = 347
Score = 258 bits (658), Expect = 2e-68
Identities = 124/237 (52%), Positives = 173/237 (72%), Gaps = 1/237 (0%)
Frame = +2
Query: 113 QPVLLNDNPVKDLTDHTEVQVDGVKITIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFA 292
+P N P ++L T + + + DL I++LG G YG+V+++RH S + A
Sbjct: 32 KPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMA 91
Query: 293 VKQIRCSMNDKQQKRILNDLNISMRSTQCPYVVISYGAMYRSGDVWICMELMDSSLDKFY 472
VK+IR ++N ++QKR+L DL+I+MR+ C Y V YGA++R GDVWICMELMD+SLDKFY
Sbjct: 92 VKRIRATVNSQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFY 151
Query: 473 KLVSDRKSFIPENLLGYMTFCVVSALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGI 652
+ V D+ IPE++LG + +V ALE+LH L VIHRDVKPSNVLINK G VK+CDFGI
Sbjct: 152 RKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGI 211
Query: 653 SGNLVNSIAET-NVGTESYLAPERINTEQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
SG LV+S+A+T + G + Y+APERIN E N GY ++SDVWSLG++++E+AIL++P+
Sbjct: 212 SGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPY 268
>sp|O09110|MP2K3_MOUSE Dual specificity mitogen-activated protein kinase kinase 3 (MAP
kinase kinase 3) (MAPKK 3) (MAPK/ERK kinase 3)
Length = 347
Score = 256 bits (655), Expect = 4e-68
Identities = 123/237 (51%), Positives = 174/237 (73%), Gaps = 1/237 (0%)
Frame = +2
Query: 113 QPVLLNDNPVKDLTDHTEVQVDGVKITIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFA 292
+P + N P ++L T + + + DL I++LG G YG+V+++RH S + A
Sbjct: 32 KPPVSNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMA 91
Query: 293 VKQIRCSMNDKQQKRILNDLNISMRSTQCPYVVISYGAMYRSGDVWICMELMDSSLDKFY 472
VK+IR ++N ++QKR+L DL+I+MR+ C Y V YGA++R GDVWICMELMD+SLDKFY
Sbjct: 92 VKRIRATVNTQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICMELMDTSLDKFY 151
Query: 473 KLVSDRKSFIPENLLGYMTFCVVSALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGI 652
+ V ++ IPE++LG + +V ALE+LH L VIHRDVKPSNVLINK G VK+CDFGI
Sbjct: 152 RKVLEKNMKIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGI 211
Query: 653 SGNLVNSIAET-NVGTESYLAPERINTEQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
SG LV+S+A+T + G + Y+APERIN E N GY ++SDVWSLG++++E+AIL++P+
Sbjct: 212 SGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPY 268
>sp|P45985|MP2K4_HUMAN Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (JNK-activating kinase 1) (c-Jun
N-terminal kinase kinase 1) (JNKK) (SAPK/ERK kinase 1)
(SEK1)
Length = 399
Score = 224 bits (571), Expect = 2e-58
Identities = 117/258 (45%), Positives = 169/258 (65%), Gaps = 15/258 (5%)
Frame = +2
Query: 92 HPPQGNIQPVLLNDNP-------VKDLTDHTEVQVDGVKIT------IAMSDLKMINKLG 232
+PP + LN NP ++ L H+ +KI+ DLK + ++G
Sbjct: 50 NPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDLKDLGEIG 109
Query: 233 EGHYGMVDEMRHPPSNLVFAVKQIRCSMNDKQQKRILNDLNISMRSTQCPYVVISYGAMY 412
G YG V++M H PS + AVK+IR ++++K+QK++L DL++ MRS+ CPY+V YGA++
Sbjct: 110 RGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPYIVQFYGALF 169
Query: 413 RSGDVWICMELMDSSLDKFYKLV-SDRKSFIPENLLGYMTFCVVSALEYLHVVLHVIHRD 589
R GD WICMELM +S DKFYK V S IPE +LG +T V AL +L L +IHRD
Sbjct: 170 REGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLKENLKIIHRD 229
Query: 590 VKPSNVLINKFGWVKVCDFGISGNLVNSIAET-NVGTESYLAPERINTEQNMVGYTIRSD 766
+KPSN+L+++ G +K+CDFGISG LV+SIA+T + G Y+APERI+ + GY +RSD
Sbjct: 230 IKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSASRQGYDVRSD 289
Query: 767 VWSLGLSVLELAILKYPF 820
VWSLG+++ ELA ++P+
Sbjct: 290 VWSLGITLYELATGRFPY 307
>sp|P47809|MP2K4_MOUSE Dual specificity mitogen-activated protein kinase kinase 4 (MAP
kinase kinase 4) (MAPKK 4) (MAPK/ERK kinase 4)
(JNK-activating kinase 1) (C-JUN N-terminal kinase
kinase 1) (JNK kinase 1) (JNKK 1) (SAPK/ERK kinase 1)
(SEK1)
Length = 397
Score = 222 bits (566), Expect = 9e-58
Identities = 107/207 (51%), Positives = 152/207 (73%), Gaps = 2/207 (0%)
Frame = +2
Query: 206 DLKMINKLGEGHYGMVDEMRHPPSNLVFAVKQIRCSMNDKQQKRILNDLNISMRSTQCPY 385
DLK + ++G G YG V++M H PS + AVK+IR ++++K+QK++L DL++ MRS+ CPY
Sbjct: 99 DLKDLGEIGRGAYGSVNKMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPY 158
Query: 386 VVISYGAMYRSGDVWICMELMDSSLDKFYKLV-SDRKSFIPENLLGYMTFCVVSALEYLH 562
+V YGA++R GD WICMELM +S DKFYK V S IPE +LG +T V AL +L
Sbjct: 159 IVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNHLK 218
Query: 563 VVLHVIHRDVKPSNVLINKFGWVKVCDFGISGNLVNSIAET-NVGTESYLAPERINTEQN 739
L +IHRD+KPSN+L+++ G +K+CDFGISG LV+SIA+T + G Y+APERI+ +
Sbjct: 219 ENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSAS 278
Query: 740 MVGYTIRSDVWSLGLSVLELAILKYPF 820
GY +RSDVWSLG+++ ELA ++P+
Sbjct: 279 RQGYDVRSDVWSLGITLYELATGRFPY 305
>sp|Q07192|MP2K2_XENLA Dual specificity mitogen-activated protein kinase kinase 2 (MAP
kinase kinase 2) (MAPKK 2) (MAPK-ERK kinase 2)
Length = 446
Score = 221 bits (562), Expect = 2e-57
Identities = 107/207 (51%), Positives = 151/207 (72%), Gaps = 2/207 (0%)
Frame = +2
Query: 206 DLKMINKLGEGHYGMVDEMRHPPSNLVFAVKQIRCSMNDKQQKRILNDLNISMRSTQCPY 385
DLK + ++G G YG V++M H PS + AVK+IR ++++K+QK++L DL++ MRS+ CPY
Sbjct: 148 DLKDLGEIGRGAYGSVNKMSHTPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPY 207
Query: 386 VVISYGAMYRSGDVWICMELMDSSLDKFYKLV-SDRKSFIPENLLGYMTFCVVSALEYLH 562
+V YGA++R GD WICMELM +S DKFYK V S IPE +LG +T V AL +L
Sbjct: 208 IVQFYGALFREGDCWICMELMATSFDKFYKYVYSFLDDVIPEEILGKITLATVKALNHLK 267
Query: 563 VVLHVIHRDVKPSNVLINKFGWVKVCDFGISGNLVNSIAET-NVGTESYLAPERINTEQN 739
L +IHRD+KPSN+L++ G +K+CDFGISG LV+SIA+T + G Y+APERI+ +
Sbjct: 268 ENLKIIHRDIKPSNILLDTNGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERIDPSAS 327
Query: 740 MVGYTIRSDVWSLGLSVLELAILKYPF 820
GY +RSDVWSLG+++ ELA ++P+
Sbjct: 328 RQGYDVRSDVWSLGITLYELATGRFPY 354
>sp|Q23977|HEP_DROME Dual specificity mitogen-activated protein kinase kinase
hemipterous (MAPKK)
Length = 1178
Score = 187 bits (476), Expect = 2e-47
Identities = 106/237 (44%), Positives = 151/237 (63%), Gaps = 2/237 (0%)
Frame = +2
Query: 116 PVLLNDNPVKDLTDHT-EVQVDGVKITIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFA 292
PV D +K + + T ++ ++G + ++DLK + LG G G V +M H SN + A
Sbjct: 165 PVSETDMKLKIIMEQTGKLNINGRQYPTDINDLKHLGDLGNGTSGNVVKMMHLSSNTIIA 224
Query: 293 VKQIRCSMNDKQQKRILNDLNISMRSTQCPYVVISYGAMYRSGDVWICMELMDSSLDKFY 472
VKQ+R + N ++ KRIL DL++ ++S C Y+V G R DVWICMELM DK
Sbjct: 225 VKQMRRTGNAEENKRILMDLDVVLKSHDCKYIVKCLGCFVRDPDVWICMELMSMCFDKLL 284
Query: 473 KLVSDRKSFIPENLLGYMTFCVVSALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGI 652
KL K +PE +LG +T V+AL YL VIHRDVKPSN+LI++ G +K+CDFGI
Sbjct: 285 KL---SKKPVPEQILGKVTVATVNALSYLKDKHGVIHRDVKPSNILIDERGNIKLCDFGI 341
Query: 653 SGNLVNSIAET-NVGTESYLAPERINTEQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
SG LV+S A T + G +Y+APERI+ ++ Y IR+DVWSLG++++ELA + P+
Sbjct: 342 SGRLVDSKANTRSAGCAAYMAPERIDPKKPK--YDIRADVWSLGITLVELATARSPY 396
>sp|Q20347|YR62_CAEEL Putative serine/threonine-protein kinase F42G10.2
Length = 363
Score = 184 bits (466), Expect = 3e-46
Identities = 95/213 (44%), Positives = 144/213 (67%), Gaps = 3/213 (1%)
Frame = +2
Query: 191 TIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFAVKQIRCS-MNDKQQKRILNDLNISMR 367
T + ++L+ + +G G++G V +MRH + + AVK+IRC+ + ++Q R+L + + ++
Sbjct: 60 TFSSANLQDLGAIGNGNFGTVYKMRHKETGKLIAVKRIRCNNIGHREQIRLLREHDTIVK 119
Query: 368 STQCPYVVISYGAMYRSGDVWICMELMDSSLDKFYKLV-SDRKSFIPENLLGYMTFCVVS 544
S + P +V YGA++ GD WICMELMD S+D YK V + S + EN++G++T C V
Sbjct: 120 SEKGPNIVKFYGAIFSEGDCWICMELMDISMDLLYKRVYMVKNSRLNENVVGHITVCTVD 179
Query: 545 ALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGISGNLVNSIAET-NVGTESYLAPER 721
AL+YL L +IHRDVKPSN+L++ G VK+CDFGI G L S A+T + G + YLAPER
Sbjct: 180 ALDYLKKELKIIHRDVKPSNILVDGTGAVKLCDFGICGQLEESFAKTHDAGCQPYLAPER 239
Query: 722 INTEQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
I + Y +RSDVWSLG+++ E+A K+P+
Sbjct: 240 ITSSDK---YDVRSDVWSLGITLYEIATGKFPY 269
>sp|P33886|WIS1_SCHPO Protein kinase wis1 (Protein kinase sty2)
Length = 605
Score = 176 bits (447), Expect = 5e-44
Identities = 93/218 (42%), Positives = 142/218 (65%), Gaps = 4/218 (1%)
Frame = +2
Query: 179 GVKITIAMSDLKMINKLGEGHYGMVDEMRHPPSNLVFAVKQIRCSMNDKQQKRILNDLNI 358
G I MS++ + +LG+G+YG+V + H P+ + A+K+IR S+ + +I+ +L+I
Sbjct: 310 GSSFRINMSEIIKLEELGKGNYGVVYKALHQPTGVTMALKEIRLSLEEATFNQIIMELDI 369
Query: 359 SMRSTQCPYVVISYGAMYRSGDVWICMELMDS-SLDKFYKLVSDRKSFIPENLLGYMTFC 535
++ PY+V YGA + G V+ICME MD+ S+DK Y E +L +
Sbjct: 370 LHKAVS-PYIVDFYGAFFVEGSVFICMEYMDAGSMDKLYA-----GGIKDEGVLARTAYA 423
Query: 536 VVSALEYLHVVLHVIHRDVKPSNVLINKFGWVKVCDFGISGNLVNSIAETNVGTESYLAP 715
VV L+ L ++IHRDVKP+NVL+N G VK+CDFG+SGNLV SI++TN+G +SY+AP
Sbjct: 424 VVQGLKTLKEEHNIIHRDVKPTNVLVNSNGQVKLCDFGVSGNLVASISKTNIGCQSYMAP 483
Query: 716 ERINT---EQNMVGYTIRSDVWSLGLSVLELAILKYPF 820
ERI ++ YT+++DVWSLGL++LE+A+ YP+
Sbjct: 484 ERIRVGGPTNGVLTYTVQADVWSLGLTILEMALGAYPY 521
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,739,614
Number of Sequences: 369166
Number of extensions: 2053534
Number of successful extensions: 8598
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6513
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6854
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8004331590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)