Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_B19
(293 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST... 95 4e-20
sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST... 85 4e-17
sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST... 84 1e-16
sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST... 81 8e-16
sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class... 74 1e-13
sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class... 73 2e-13
sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (... 72 4e-13
sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-si... 71 7e-13
sp|Q21355|GST4_CAEEL Glutathione S-transferase 4 (GST class... 70 2e-12
sp|P91254|GST8_CAEEL Probable glutathione S-transferase 8 (... 68 6e-12
>sp|P26624|GST28_SCHJA Glutathione S-transferase 28 kDa (GST 28) (SJ28 antigen) (GST
class-mu)
Length = 206
Score = 95.1 bits (235), Expect = 4e-20
Identities = 48/102 (47%), Positives = 68/102 (66%), Gaps = 5/102 (4%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKK-----LTQSMAI 166
GRAE IR++L A+ VEFED R+ + W +KPT P G+LP+V+ K+ +++S+AI
Sbjct: 10 GRAEPIRMILVAAGVEFEDERIEFQDWPKIKPTIPGGRLPIVKITDKRGDVKTMSESLAI 69
Query: 167 ARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAP 292
AR IARK+ MG+ D EYY+IE+ IGQ+ DV +K P
Sbjct: 70 ARFIARKHNMMGDTDDEYYIIEKMIGQVEDVESEYHKTLIKP 111
>sp|P09792|GST28_SCHMA Glutathione S-transferase 28 kDa (GST 28) (SM28 antigen)
(Protective 28 kDa antigen) (GST class-mu)
Length = 211
Score = 85.1 bits (209), Expect = 4e-17
Identities = 44/102 (43%), Positives = 64/102 (62%), Gaps = 5/102 (4%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG-----KKLTQSMAI 166
GRAE IR+ L A+ V++ED R++ + W +KPT P G+LP V+ K + +S+AI
Sbjct: 15 GRAESIRMTLVAAGVDYEDERISFQDWPKIKPTIPGGRLPAVKVTDDHGHVKWMLESLAI 74
Query: 167 ARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAP 292
AR +A+K+ MGE D+EYY +E+ IGQ DV +K P
Sbjct: 75 ARYMAKKHHMMGETDEEYYSVEKLIGQAEDVEHEYHKTLMKP 116
>sp|P30114|GST28_SCHHA Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 83.6 bits (205), Expect = 1e-16
Identities = 43/102 (42%), Positives = 64/102 (62%), Gaps = 5/102 (4%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG-----KKLTQSMAI 166
GRAE IR+ L A+ V +ED R++ + W +KPT P G+LP V+ K + +S+AI
Sbjct: 15 GRAESIRMTLVAAGVNYEDERISFQDWPKIKPTIPGGRLPAVKITDNHGHVKWMLESLAI 74
Query: 167 ARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAP 292
AR +A+K+ MGE D+EYY +E+ IGQ+ D+ +K P
Sbjct: 75 ARYMAKKHHMMGETDEEYYNVEKLIGQVEDLEHEYHKTLMKP 116
>sp|P30113|GST28_SCHBO Glutathione S-transferase 28 kDa (GST 28) (GST class-mu)
Length = 211
Score = 80.9 bits (198), Expect = 8e-16
Identities = 42/102 (41%), Positives = 62/102 (60%), Gaps = 5/102 (4%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEG-----KKLTQSMAI 166
GRAE IR+ L A+ V +ED R++ + W +KPT P G+LP V+ K + +S+AI
Sbjct: 15 GRAESIRMTLVAAGVNYEDERISFQDWPKIKPTIPGGRLPAVKITDNHGHVKWMVESLAI 74
Query: 167 ARAIARKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAP 292
AR +A+K+ MG ++EYY +E+ IGQ D+ YK P
Sbjct: 75 ARYMAKKHHMMGGTEEEYYNVEKLIGQAEDLEHEYYKTLMKP 116
>sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class-sigma) (CeGST3)
Length = 207
Score = 73.6 bits (179), Expect = 1e-13
Identities = 36/96 (37%), Positives = 59/96 (61%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAIA 181
G AEI R + + VEFED R+ +E++ +KPT P+G++P++ +G + +QS AIAR +A
Sbjct: 13 GLAEISRQLFHMAGVEFEDERINEEKFSQLKPTFPSGQVPILCIDGAQFSQSTAIARYLA 72
Query: 182 RKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFA 289
RK+GF+G+ +E + + D +E K A
Sbjct: 73 RKFGFVGQTAEEELQADEVVDTFKDFIESFRKFVIA 108
>sp|P46429|GST2_MANSE Glutathione S-transferase 2 (GST class-sigma)
Length = 203
Score = 72.8 bits (177), Expect = 2e-13
Identities = 33/71 (46%), Positives = 49/71 (69%)
Frame = +2
Query: 47 EFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAIARKYGFMGEDDKEYYL 226
EFED R+ EQW + KP TP G++PV+E +GKK QS+AI+R + RKYG G D +E +
Sbjct: 27 EFEDHRVEYEQWPEFKPNTPFGQMPVLEIDGKKYAQSLAISRYLGRKYGLAGNDIEEDFE 86
Query: 227 IERAIGQMVDV 259
I++ + + D+
Sbjct: 87 IDQIVDFVNDI 97
>sp|P91253|GST7_CAEEL Probable glutathione S-transferase 7 (GST class-sigma)
Length = 206
Score = 72.0 bits (175), Expect = 4e-13
Identities = 38/87 (43%), Positives = 53/87 (60%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAIA 181
G EI R +L + +FED R+ KE+W VKP+TP G+LP++E +GK L QS AIAR +A
Sbjct: 13 GAGEIARQILAYAGQDFEDNRIPKEEWPAVKPSTPFGQLPLLEVDGKVLAQSHAIARYLA 72
Query: 182 RKYGFMGEDDKEYYLIERAIGQMVDVL 262
R++G G+ E + Q D L
Sbjct: 73 RQFGINGKCAWEEAQVNSVADQFKDYL 99
>sp|P46088|GST_OMMSL Glutathione S-transferase (GST class-sigma)
Length = 203
Score = 71.2 bits (173), Expect = 7e-13
Identities = 36/97 (37%), Positives = 56/97 (57%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAIA 181
GRAE+ R VL A EF D + W ++K T + +PV++ +G K++QSM IAR +A
Sbjct: 13 GRAELCRFVLAAHGEEFTDRVVEMADWPNLKATMYSNAMPVLDIDGTKMSQSMCIARHLA 72
Query: 182 RKYGFMGEDDKEYYLIERAIGQMVDVLEGMYKIYFAP 292
R++G G+ E Y ++ + D+ + KI FAP
Sbjct: 73 REFGLDGKTSLEKYRVDEITETLQDIFNDVVKIKFAP 109
>sp|Q21355|GST4_CAEEL Glutathione S-transferase 4 (GST class-sigma) (CeGST1)
Length = 207
Score = 69.7 bits (169), Expect = 2e-12
Identities = 32/85 (37%), Positives = 54/85 (63%)
Frame = +2
Query: 8 AEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAIARK 187
AE IR++ V +ED R++ E+W +KPTTP G+LP+++ +G++ QSM+I R +ARK
Sbjct: 15 AEPIRIMFAMLNVPYEDYRVSVEEWSKLKPTTPFGQLPILQVDGEQFGQSMSITRYLARK 74
Query: 188 YGFMGEDDKEYYLIERAIGQMVDVL 262
+G G+ +E + + Q D +
Sbjct: 75 FGLAGKTAEEEAYADSIVDQYRDFI 99
>sp|P91254|GST8_CAEEL Probable glutathione S-transferase 8 (GST class-sigma)
Length = 206
Score = 68.2 bits (165), Expect = 6e-12
Identities = 32/68 (47%), Positives = 47/68 (69%)
Frame = +2
Query: 2 GRAEIIRLVLKASKVEFEDVRLTKEQWVDVKPTTPTGKLPVVEYEGKKLTQSMAIARAIA 181
G E+IR + + FED R++ E+W VKPTTP G+LP++E +GK L QS +IAR +A
Sbjct: 13 GAGEVIRQIFVYAGQSFEDHRISIEEWAAVKPTTPFGQLPLLEVDGKVLPQSHSIARFLA 72
Query: 182 RKYGFMGE 205
R++G G+
Sbjct: 73 RQFGINGK 80
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,148,330
Number of Sequences: 369166
Number of extensions: 678971
Number of successful extensions: 2245
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2228
length of database: 68,354,980
effective HSP length: 67
effective length of database: 55,977,735
effective search space used: 1679332050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)