Planarian EST Database


Dr_sW_003_A18

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_003_A18
         (861 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P13230|GRP33_ARTSA  Glycine-rich protein GRP33                 126   9e-29
sp|Q60749|SAM68_MOUSE  KH domain containing, RNA binding, si...   117   5e-26
sp|Q07666|SAM68_HUMAN  KH domain containing, RNA binding, si...   117   5e-26
sp|Q91V33|SAM68_RAT  KH domain containing, RNA binding, sign...   117   5e-26
sp|Q8UUW7|SAM68_CHICK  KH domain containing, RNA binding, si...   117   5e-26
sp|O01367|HOW_DROME  Held out wings protein (KH-domain prote...    83   1e-15
sp|Q17339|GLD1_CAEEL  Female germline-specific tumor suppres...    77   6e-14
sp|Q15637|SF01_HUMAN  Splicing factor 1 (Zinc finger protein...    51   4e-06
sp|Q64213|SF01_MOUSE  Splicing factor 1 (Zinc finger protein...    50   6e-06
sp|P42083|YKCA_CAEEL  Hypothetical protein B0280.11 in chrom...    48   4e-05
>sp|P13230|GRP33_ARTSA Glycine-rich protein GRP33
          Length = 308

 Score =  126 bits (316), Expect = 9e-29
 Identities = 62/125 (49%), Positives = 88/125 (70%), Gaps = 7/125 (5%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           +PV+ +P +NF+GKLLGP G T+K +Q++T TK+SILG GS+R+  KE+EL NSGD KY 
Sbjct: 80  LPVDQFPKYNFLGKLLGPGGSTMKQLQDETMTKISILGRGSMRDRNKEEELRNSGDVKYA 139

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIP--DPN-----EQAPGQMPMVTPLM 624
           HL ++LH++I SI +P+EA+  ++YAL E+KK + P  DPN         G M  +  +M
Sbjct: 140 HLNEQLHIEIISIASPAEAHARMAYALTEIKKYITPEEDPNYMMMAGHGAGPMMGMGGMM 199

Query: 625 GGPNP 639
           GGP P
Sbjct: 200 GGPGP 204
>sp|Q60749|SAM68_MOUSE KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score =  117 bits (292), Expect = 5e-26
 Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 1/122 (0%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           IPV+ YP FNFVGK+LGP G T+K +QE+T  K+S+LG GS+R+  KE+EL   GDPKY 
Sbjct: 162 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 221

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD-PNEQAPGQMPMVTPLMGGPNPG 642
           HL   LH+ I+  G P EAY  +++A+ EVKK ++PD  ++    Q   ++ L G P P 
Sbjct: 222 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPS 281

Query: 643 FG 648
            G
Sbjct: 282 RG 283
>sp|Q07666|SAM68_HUMAN KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score =  117 bits (292), Expect = 5e-26
 Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 1/122 (0%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           IPV+ YP FNFVGK+LGP G T+K +QE+T  K+S+LG GS+R+  KE+EL   GDPKY 
Sbjct: 162 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 221

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD-PNEQAPGQMPMVTPLMGGPNPG 642
           HL   LH+ I+  G P EAY  +++A+ EVKK ++PD  ++    Q   ++ L G P P 
Sbjct: 222 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPS 281

Query: 643 FG 648
            G
Sbjct: 282 RG 283
>sp|Q91V33|SAM68_RAT KH domain containing, RNA binding, signal transduction associated
           protein 1 (p21 Ras GTPase-activating protein-associated
           p62) (GAP-associated tyrosine phosphoprotein p62)
           (Src-associated in mitosis 68 kDa protein) (Sam68) (p68)
          Length = 443

 Score =  117 bits (292), Expect = 5e-26
 Identities = 59/122 (48%), Positives = 81/122 (66%), Gaps = 1/122 (0%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           IPV+ YP FNFVGK+LGP G T+K +QE+T  K+S+LG GS+R+  KE+EL   GDPKY 
Sbjct: 162 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 221

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPD-PNEQAPGQMPMVTPLMGGPNPG 642
           HL   LH+ I+  G P EAY  +++A+ EVKK ++PD  ++    Q   ++ L G P P 
Sbjct: 222 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPS 281

Query: 643 FG 648
            G
Sbjct: 282 RG 283
>sp|Q8UUW7|SAM68_CHICK KH domain containing, RNA binding, signal transduction associated
           protein 1 (Src-associated in mitosis 68 kDa protein)
           (Sam68)
          Length = 433

 Score =  117 bits (292), Expect = 5e-26
 Identities = 62/137 (45%), Positives = 83/137 (60%), Gaps = 10/137 (7%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           IPV+ YP FNFVGK+LGP G T+K +QE+T  K+S+LG GS+R+  KE+EL   GDPKY 
Sbjct: 145 IPVKQYPKFNFVGKILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYA 204

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDPNEQAPGQ----------MPMVT 615
           HL   LH+ I+  G P EAY  +++A+ EVKK ++PD  +    +          +P  T
Sbjct: 205 HLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPT 264

Query: 616 PLMGGPNPGFGAMRTQP 666
              GGP  G GA    P
Sbjct: 265 RGRGGPVRGRGAAPPPP 281
>sp|O01367|HOW_DROME Held out wings protein (KH-domain protein KH93F) (Putative
           RNA-binding protein) (Muscle-specific protein) (Wings
           held out protein) (Struthio protein) (Quaking-related
           93F)
          Length = 405

 Score = 82.8 bits (203), Expect = 1e-15
 Identities = 41/105 (39%), Positives = 66/105 (62%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           +PV  +P+FNFVG++LGP G T K ++++T  K+ + G GS+R+ +KE    N G P + 
Sbjct: 143 VPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKED--ANRGKPNWE 200

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDPNEQAPGQ 600
           HL D LH+ I      + A   ++ A+AEV+K+++P    QA G+
Sbjct: 201 HLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVP----QAEGE 241
>sp|Q17339|GLD1_CAEEL Female germline-specific tumor suppressor gld-1 (Defective in germ
           line development protein 1)
          Length = 463

 Score = 77.0 bits (188), Expect = 6e-14
 Identities = 35/98 (35%), Positives = 58/98 (59%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYN 465
           +P   YP++NFVG++LGP G T K +++ T  K+ + G GS+R  +K +E  + G   + 
Sbjct: 211 VPKNEYPDYNFVGRILGPRGMTAKQLEQDTGCKIMVRGKGSMR--DKSKESAHRGKANWE 268

Query: 466 HLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVMIPDP 579
           HL D LH+ +      +  +  +  AL +VKK++IP P
Sbjct: 269 HLEDDLHVLVQCEDTENRVHIKLQAALEQVKKLLIPAP 306
>sp|Q15637|SF01_HUMAN Splicing factor 1 (Zinc finger protein 162) (Transcription factor
           ZFM1) (Zinc finger gene in MEN1 locus) (Mammalian branch
           point binding protein mBBP) (BBP)
          Length = 639

 Score = 51.2 bits (121), Expect = 4e-06
 Identities = 33/107 (30%), Positives = 57/107 (53%), Gaps = 6/107 (5%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPE---KEQELLNSGDP 456
           IP + YP  NFVG L+GP G TLK ++++ N K+ I G GS++  +   K+ ++L   D 
Sbjct: 142 IPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVKEGKVGRKDGQMLPGED- 200

Query: 457 KYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVM---IPDPNEQ 588
                 + LH  + +  N  E   N+  A+ +++ ++   I  P +Q
Sbjct: 201 ------EPLHALVTA--NTME---NVKKAVEQIRNILKQGIETPEDQ 236
>sp|Q64213|SF01_MOUSE Splicing factor 1 (Zinc finger protein 162) (Transcription factor
           ZFM1) (mZFM) (Zinc finger gene in MEN1 locus) (Mammalian
           branch point binding protein mBBP) (BBP) (CW17)
          Length = 653

 Score = 50.4 bits (119), Expect = 6e-06
 Identities = 33/107 (30%), Positives = 56/107 (52%), Gaps = 6/107 (5%)
 Frame = +1

Query: 286 IPVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPE---KEQELLNSGDP 456
           IP + YP  NFVG L+GP G TLK ++++ N K+ I G GS+   +   K+ ++L   D 
Sbjct: 142 IPQDEYPEINFVGLLIGPRGNTLKNIEKECNAKIMIRGKGSVEEGKVGRKDGQMLPGED- 200

Query: 457 KYNHLRDRLHLQIDSIGNPSEAYYNISYALAEVKKVM---IPDPNEQ 588
                 + LH  + +  N  E   N+  A+ +++ ++   I  P +Q
Sbjct: 201 ------EPLHALVTA--NTME---NVKKAVEQIRNILKQGIETPEDQ 236
>sp|P42083|YKCA_CAEEL Hypothetical protein B0280.11 in chromosome III
          Length = 634

 Score = 47.8 bits (112), Expect = 4e-05
 Identities = 28/93 (30%), Positives = 49/93 (52%)
 Frame = +1

Query: 289 PVEAYPNFNFVGKLLGPAGKTLKTVQEQTNTKMSILGAGSLRNPEKEQELLNSGDPKYNH 468
           P E   N N VG+L+GP G T++ +++    K+ I G G  ++  KE+ L       + H
Sbjct: 520 PPETANNTNPVGRLIGPRGMTIRQLEKDLGCKLFIRGKGCTKDDAKEERLRER--VGWEH 577

Query: 469 LRDRLHLQIDSIGNPSEAYYNISYALAEVKKVM 567
           L++ +H+ I    +  EA    S  L+ +KK++
Sbjct: 578 LKEPIHVMISVRSDSEEA---ASEKLSSIKKML 607
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.314    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,139,318
Number of Sequences: 369166
Number of extensions: 1532079
Number of successful extensions: 2912
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2819
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2905
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)