Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_A11
(484 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P37167|ACTP_ACACA Actophorin 69 4e-12
sp|Q03048|COFI_YEAST Cofilin 59 6e-09
sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g0... 59 8e-09
sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2) 57 3e-08
sp|Q8SAG3|ADF_VITVI Actin-depolymerizing factor (ADF) 57 3e-08
sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) ... 56 4e-08
sp|P54706|COFI_DICDI Cofilin 56 5e-08
sp|Q9ZSK2|ADF6_ARATH Actin-depolymerizing factor 6 (ADF-6) ... 56 5e-08
sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) ... 56 5e-08
sp|Q9FVI2|ADF1_PETHY Actin-depolymerizing factor 1 (ADF 1) 55 6e-08
>sp|P37167|ACTP_ACACA Actophorin
Length = 138
Score = 69.3 bits (168), Expect = 4e-12
Identities = 35/126 (27%), Positives = 62/126 (49%), Gaps = 8/126 (6%)
Frame = +2
Query: 23 NYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE-- 196
N +KL H+ +YV + +N ++ G YE FK + E + YA++DYE
Sbjct: 15 NELKLGHQHRYVTFKMNASNTEVVVEHVGGPNATYEDFKSQLPERDCR---YAIFDYEFQ 71
Query: 197 ------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKE 358
+ FI W P++ I S+M+ ++K I ++VG++ +++A EI E + E
Sbjct: 72 VDGGQRNKITFILWAPDSAPIKSKMMYTSTKDSIKKKLVGIQVEVQATDAAEISEDAVSE 131
Query: 359 KTASKV 376
+ V
Sbjct: 132 RAKKDV 137
>sp|Q03048|COFI_YEAST Cofilin
Length = 143
Score = 58.9 bits (141), Expect = 6e-09
Identities = 32/125 (25%), Positives = 61/125 (48%), Gaps = 10/125 (8%)
Frame = +2
Query: 23 NYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE-- 196
N +KL K++++++GLN K I+ ET Y+ F + + E + YA+YD+E
Sbjct: 17 NDLKLGKKYKFILFGLNDAKTEIVVKETST-DPSYDAFLEKLPEN---DCLYAIYDFEYE 72
Query: 197 --------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVI 352
+ F +W P+T + S+M+ A+SK + + GV ++ E+ +
Sbjct: 73 INGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEVSYDSV 132
Query: 353 KEKTA 367
E+ +
Sbjct: 133 LERVS 137
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
Length = 140
Score = 58.5 bits (140), Expect = 8e-09
Identities = 29/118 (24%), Positives = 65/118 (55%), Gaps = 9/118 (7%)
Frame = +2
Query: 29 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGT-- 202
+K + ++++++ ++++ +++ + G+ YE F + I E E YA+YDY+ T
Sbjct: 21 LKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPE---DECRYAVYDYDFTTP 77
Query: 203 -------LAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIK 355
+ FI+W P+T ++ S+M+ A+SK + G++ +++A E+ +IK
Sbjct: 78 ENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSEMSLDIIK 135
>sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2)
Length = 143
Score = 56.6 bits (135), Expect = 3e-08
Identities = 28/120 (23%), Positives = 61/120 (50%), Gaps = 9/120 (7%)
Frame = +2
Query: 29 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE---- 196
+K + ++++IY + +++ ++ LG P E ++ A E YA+YD++
Sbjct: 21 LKAKRTYRFIIYKIEEKQKEVV---VEKLGEPTESYEDFTAGLPADECRYAVYDFDFMTK 77
Query: 197 -----GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 361
+ FI+W P+T ++ S+MI A+SK + G++ +++A E+ V + +
Sbjct: 78 ENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPTEMGLDVFRSR 137
>sp|Q8SAG3|ADF_VITVI Actin-depolymerizing factor (ADF)
Length = 143
Score = 56.6 bits (135), Expect = 3e-08
Identities = 30/120 (25%), Positives = 64/120 (53%), Gaps = 9/120 (7%)
Frame = +2
Query: 29 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYE---- 196
+K + +YVI+ ++++K ++ +TG +++F + E + YA+YD++
Sbjct: 25 LKRKKVHRYVIFKIDEKKKEVVVEKTGGPAESFDEFAAALPENDCR---YAVYDFDFVTS 81
Query: 197 -----GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 361
+ FI+W P++ +I ++M+ A SK + GV +I+A E+D V++E+
Sbjct: 82 ENCQKSKIFFIAWSPDSSRIRAKMLYATSKERFRRELDGVHYEIQATDPTEMDLEVLRER 141
>sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) (ADF)
Length = 165
Score = 56.2 bits (134), Expect = 4e-08
Identities = 37/116 (31%), Positives = 58/116 (50%), Gaps = 14/116 (12%)
Frame = +2
Query: 56 VIYGLNKEKNRIIKLE-----TGDLGTPYEKFKQHIIETYAQEGC-YALYDY-------- 193
VI+ L+ +K II E GD+G +H +E ++ C YALYD
Sbjct: 36 VIFCLSPDKKCIIVEEGKEILVGDVGVTVTDPFKHFVEMLPEKDCRYALYDASFETKESK 95
Query: 194 EGTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 361
+ L F W PE + S+MI A+SK I + G+K + +AN ++++ + I EK
Sbjct: 96 KEELMFFLWAPEQAPLKSKMIYASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|P54706|COFI_DICDI Cofilin
Length = 137
Score = 55.8 bits (133), Expect = 5e-08
Identities = 31/128 (24%), Positives = 60/128 (46%), Gaps = 8/128 (6%)
Frame = +2
Query: 2 PSVEEYLNYIKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYA 181
P+ N +KL K+ +IY ++ + II T G +++F + + E + Y
Sbjct: 9 PNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCLPENECR---YV 65
Query: 182 LYDYE--------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI 337
+ DY+ + F++W P+T I +M+ +SK + VG++ +I+ E+
Sbjct: 66 VLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEIQGTDASEV 125
Query: 338 DESVIKEK 361
+S EK
Sbjct: 126 KDSCFYEK 133
>sp|Q9ZSK2|ADF6_ARATH Actin-depolymerizing factor 6 (ADF-6) (AtADF6)
Length = 146
Score = 55.8 bits (133), Expect = 5e-08
Identities = 29/128 (22%), Positives = 67/128 (52%), Gaps = 11/128 (8%)
Frame = +2
Query: 11 EEYLNYIKLQHK--FQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYAL 184
E +++LQ K +YV++ +++ K ++ +TG+ Y+ F + + + YA+
Sbjct: 20 ESKTTFLELQRKKTHRYVVFKIDESKKEVVVEKTGNPTESYDDFLASLPDNDCR---YAV 76
Query: 185 YDYE---------GTLAFISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEI 337
YD++ + F +W P T I ++++ + SK +++ + G+ +I+A E+
Sbjct: 77 YDFDFVTSENCQKSKIFFFAWSPSTSGIRAKVLYSTSKDQLSRELQGIHYEIQATDPTEV 136
Query: 338 DESVIKEK 361
D V++E+
Sbjct: 137 DLEVLRER 144
>sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) (AtADF1)
Length = 139
Score = 55.8 bits (133), Expect = 5e-08
Identities = 29/120 (24%), Positives = 62/120 (51%), Gaps = 9/120 (7%)
Frame = +2
Query: 29 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGTLA 208
+K + ++++Y + +++ +++ + G YE+F + A E YA+YD++ A
Sbjct: 21 LKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACL---PADECRYAIYDFDFVTA 77
Query: 209 ---------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 361
FI+W P+ K+ S+MI A+SK + G++ +++A E+D V + +
Sbjct: 78 ENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFRSR 137
>sp|Q9FVI2|ADF1_PETHY Actin-depolymerizing factor 1 (ADF 1)
Length = 139
Score = 55.5 bits (132), Expect = 6e-08
Identities = 28/120 (23%), Positives = 63/120 (52%), Gaps = 9/120 (7%)
Frame = +2
Query: 29 IKLQHKFQYVIYGLNKEKNRIIKLETGDLGTPYEKFKQHIIETYAQEGCYALYDYEGTLA 208
+K + ++++Y + +++ +++ + G+ YE F + E + YA+YD++ A
Sbjct: 21 LKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASLPENECR---YAVYDFDFVTA 77
Query: 209 ---------FISWIPETMKINSRMIMAASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 361
FI+W P+T ++ S+MI A+SK + G++ +++A E+ VI+ +
Sbjct: 78 ENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVELQACDPTEMGLDVIQSR 137
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,339,837
Number of Sequences: 369166
Number of extensions: 799164
Number of successful extensions: 1893
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1850
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1888
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2871696580
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)