Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_A09
(853 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q62245|SOS1_MOUSE Son of sevenless protein homolog 1 (SO... 59 2e-08
sp|Q07890|SOS2_HUMAN Son of sevenless protein homolog 2 (SO... 56 1e-07
sp|Q07889|SOS1_HUMAN Son of sevenless protein homolog 1 (SO... 55 2e-07
sp|Q02384|SOS2_MOUSE Son of sevenless protein homolog 2 (SO... 54 7e-07
sp|P26675|SOS_DROME Son of sevenless protein 52 3e-06
sp|P27671|GNRP_MOUSE Guanine nucleotide-releasing protein (... 41 0.004
sp|P28818|GNRP_RAT Guanine nucleotide-releasing protein (GN... 41 0.004
sp|P04821|CDC25_YEAST Cell division control protein 25 39 0.014
sp|Q13972|GNRP_HUMAN Guanine nucleotide-releasing protein (... 39 0.018
sp|P14771|SDC25_YEAST Guanine nucleotide exchange factor SDC25 39 0.024
>sp|Q62245|SOS1_MOUSE Son of sevenless protein homolog 1 (SOS-1) (mSOS-1)
Length = 1319
Score = 58.9 bits (141), Expect = 2e-08
Identities = 46/149 (30%), Positives = 71/149 (47%), Gaps = 1/149 (0%)
Frame = +2
Query: 5 IIRKYKTYKQIFNNLRKEHENGKGHKPSLYTQEYENNIQKIEEADPPVPCVPFIAFGV-T 181
+ R T++QI + +K E L Y+ + K+ +PP CVPF FG+
Sbjct: 883 VYRLDHTFEQIPSRQKKILEEAH----ELSEDHYKKYLAKLRSINPP--CVPF--FGIYL 934
Query: 182 TKLIHTALKSPNTITTDSGNVLINFRKHRRLSQIVERYLSFQRLPYQFQKEPMISTFLCN 361
T ++ T +P + G LINF K RR+++I +Q PY + EP I F N
Sbjct: 935 TNILKTEEGNPEVLRRH-GKELINFSKRRRVAEITGEIQQYQNQPYCLRVEPDIKRFFEN 993
Query: 362 LDPLKSVNAKNDDDFERIMFEISEKYEPR 448
L+P+ + K D+ +F S + EPR
Sbjct: 994 LNPMGNSMEKEFTDY---LFNKSLEIEPR 1019
>sp|Q07890|SOS2_HUMAN Son of sevenless protein homolog 2 (SOS-2)
Length = 1332
Score = 56.2 bits (134), Expect = 1e-07
Identities = 37/121 (30%), Positives = 59/121 (48%)
Frame = +2
Query: 89 LYTQEYENNIQKIEEADPPVPCVPFIAFGVTTKLIHTALKSPNTITTDSGNVLINFRKHR 268
L ++ + K++ +PP CVPF FG+ I + N G LINF K R
Sbjct: 905 LSQDHFKKYLVKLKSINPP--CVPF--FGIYLTNILKTEEGNNDFLKRKGKDLINFSKRR 960
Query: 269 RLSQIVERYLSFQRLPYQFQKEPMISTFLCNLDPLKSVNAKNDDDFERIMFEISEKYEPR 448
++++I +Q PY + EP + F NL+P+ S + K D+ +F S + EPR
Sbjct: 961 KVAEITGEIQQYQNQPYCLRIEPDMRRFFENLNPMGSASEKEFTDY---LFNKSLEIEPR 1017
Query: 449 D 451
+
Sbjct: 1018 N 1018
>sp|Q07889|SOS1_HUMAN Son of sevenless protein homolog 1 (SOS-1)
Length = 1333
Score = 55.1 bits (131), Expect = 2e-07
Identities = 44/150 (29%), Positives = 71/150 (47%), Gaps = 1/150 (0%)
Frame = +2
Query: 5 IIRKYKTYKQIFNNLRKEHENGKGHKPSLYTQEYENNIQKIEEADPPVPCVPFIAFGV-T 181
+ R T++QI + +K E L Y+ + K+ +PP CVPF FG+
Sbjct: 883 VYRLDHTFEQIPSRQKKILEEAH----ELSEDHYKKYLAKLRSINPP--CVPF--FGIYL 934
Query: 182 TKLIHTALKSPNTITTDSGNVLINFRKHRRLSQIVERYLSFQRLPYQFQKEPMISTFLCN 361
T ++ T +P + G LINF K R++++I +Q PY + E I F N
Sbjct: 935 TNILKTEEGNPEVLKRH-GKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFEN 993
Query: 362 LDPLKSVNAKNDDDFERIMFEISEKYEPRD 451
L+P+ + K D+ +F S + EPR+
Sbjct: 994 LNPMGNSMEKEFTDY---LFNKSLEIEPRN 1020
>sp|Q02384|SOS2_MOUSE Son of sevenless protein homolog 2 (SOS-2) (mSOS-2)
Length = 1297
Score = 53.5 bits (127), Expect = 7e-07
Identities = 35/121 (28%), Positives = 59/121 (48%)
Frame = +2
Query: 89 LYTQEYENNIQKIEEADPPVPCVPFIAFGVTTKLIHTALKSPNTITTDSGNVLINFRKHR 268
L ++ + K++ +PP CVPF FG+ I + + G LINF K R
Sbjct: 870 LSQDHFKKYLVKLKSINPP--CVPF--FGIYLTNILKTEEGNSDFLKRKGKDLINFSKRR 925
Query: 269 RLSQIVERYLSFQRLPYQFQKEPMISTFLCNLDPLKSVNAKNDDDFERIMFEISEKYEPR 448
++++I +Q PY + EP + F NL+P+ ++ K D+ +F S + EPR
Sbjct: 926 KVAEITGEIQQYQNQPYCLRTEPEMRRFFENLNPMGILSEKEFTDY---LFNKSLEIEPR 982
Query: 449 D 451
+
Sbjct: 983 N 983
>sp|P26675|SOS_DROME Son of sevenless protein
Length = 1596
Score = 51.6 bits (122), Expect = 3e-06
Identities = 34/120 (28%), Positives = 58/120 (48%), Gaps = 1/120 (0%)
Frame = +2
Query: 92 YTQEYENNIQKIEEADPPVPCVPFIAFG-VTTKLIHTALKSPNTITTDSGNVLINFRKHR 268
+ ++Y+ ++ I PCVPF FG T ++H +P+ + LINF K R
Sbjct: 960 HLKKYQERLRSINP-----PCVPF--FGRYLTNILHLEEGNPDLLANTE---LINFSKRR 1009
Query: 269 RLSQIVERYLSFQRLPYQFQKEPMISTFLCNLDPLKSVNAKNDDDFERIMFEISEKYEPR 448
++++I+ +Q PY +E I F LDP ++ K D+ ++ S + EPR
Sbjct: 1010 KVAEIIGEIQQYQNQPYCLNEESTIRQFFEQLDPFNGLSDKQMSDY---LYNESLRIEPR 1066
>sp|P27671|GNRP_MOUSE Guanine nucleotide-releasing protein (GNRP) (Ras-specific nucleotide
exchange factor CDC25) (CDC25Mm)
Length = 1262
Score = 41.2 bits (95), Expect = 0.004
Identities = 25/84 (29%), Positives = 43/84 (51%)
Frame = +2
Query: 104 YENNIQKIEEADPPVPCVPFIAFGVTTKLIHTALKSPNTITTDSGNVLINFRKHRRLSQI 283
++N + + DPP CVP++ +T L+ +PN T+ G L+NF K R +S I
Sbjct: 1161 FKNLRESLRNCDPP--CVPYLGMYLTD-LVFIEEGTPNY--TEDG--LVNFSKMRMISHI 1213
Query: 284 VERYLSFQRLPYQFQKEPMISTFL 355
+ FQ+ Y+ +P + +L
Sbjct: 1214 IREIRQFQQTTYKIDPQPKVIQYL 1237
>sp|P28818|GNRP_RAT Guanine nucleotide-releasing protein (GNRP) (P140 Ras-GRF)
Length = 1244
Score = 41.2 bits (95), Expect = 0.004
Identities = 25/84 (29%), Positives = 44/84 (52%)
Frame = +2
Query: 104 YENNIQKIEEADPPVPCVPFIAFGVTTKLIHTALKSPNTITTDSGNVLINFRKHRRLSQI 283
++N + + DPP CVP++ +T L +PN T+ G L+NF K R +S I
Sbjct: 1143 FKNLRETLRNCDPP--CVPYLGMYLTD-LAFLEEGTPNY--TEDG--LVNFSKMRMISHI 1195
Query: 284 VERYLSFQRLPYQFQKEPMISTFL 355
+ FQ+ Y+ + +P ++ +L
Sbjct: 1196 IREIRQFQQTTYKIEPQPKVTQYL 1219
>sp|P04821|CDC25_YEAST Cell division control protein 25
Length = 1589
Score = 39.3 bits (90), Expect = 0.014
Identities = 30/102 (29%), Positives = 53/102 (51%), Gaps = 1/102 (0%)
Frame = +2
Query: 146 VPCVPFIAFGV-TTKLIHTALKSPNTITTDSGNVLINFRKHRRLSQIVERYLSFQRLPYQ 322
V CVPF FGV + L T + +P+ + + +INF K +++ IVE +SF+R Y+
Sbjct: 1453 VACVPF--FGVYLSDLTFTFVGNPDFLHNSTN--IINFSKRTKIANIVEEIISFKRFHYK 1508
Query: 323 FQKEPMISTFLCNLDPLKSVNAKNDDDFERIMFEISEKYEPR 448
++ I T + + +N E+ +++S + EPR
Sbjct: 1509 LKRLDDIQTVI-------EASLENVPHIEK-QYQLSLQVEPR 1542
>sp|Q13972|GNRP_HUMAN Guanine nucleotide-releasing protein (GNRP) (Ras-specific nucleotide
exchange factor CDC25) (Ras-specific guanine
nucleotide-releasing factor)
Length = 1275
Score = 38.9 bits (89), Expect = 0.018
Identities = 29/115 (25%), Positives = 55/115 (47%)
Frame = +2
Query: 104 YENNIQKIEEADPPVPCVPFIAFGVTTKLIHTALKSPNTITTDSGNVLINFRKHRRLSQI 283
++N + ++ DPP CVP++ +T L +PN T+ G L+NF K R +S I
Sbjct: 1174 FKNLREALKNCDPP--CVPYLGMYLTD-LAFIEEGTPNY--TEDG--LVNFSKMRMISHI 1226
Query: 284 VERYLSFQRLPYQFQKEPMISTFLCNLDPLKSVNAKNDDDFERIMFEISEKYEPR 448
+ FQ+ Y+ + + ++ +L + + E ++E S + EP+
Sbjct: 1227 IREIRQFQQTAYKIEHQAKVTQYLLDQSFVMD---------EESLYESSLRIEPK 1272
>sp|P14771|SDC25_YEAST Guanine nucleotide exchange factor SDC25
Length = 1252
Score = 38.5 bits (88), Expect = 0.024
Identities = 44/188 (23%), Positives = 77/188 (40%), Gaps = 9/188 (4%)
Frame = +2
Query: 5 IIRKYKTYKQIFNNLRKEHEN-GKGHKPSLYTQEYENNIQKIEEADPPVPCVPFIAFGV- 178
I R KT++ + R ++ K P Y N ++ + A PCVPF FGV
Sbjct: 1057 IYRLEKTWQAVIPQTRDLLQSLNKLMDPKKNFINYRNELKSLHSA----PCVPF--FGVY 1110
Query: 179 TTKLIHTALKSPNTITTDSG-------NVLINFRKHRRLSQIVERYLSFQRLPYQFQKEP 337
+ L T +P+ + + G INF K RL I++ + F++ Y F K+
Sbjct: 1111 LSDLTFTDSGNPDYLVLEHGLKGVHDEKKYINFNKRSRLVDILQEIIYFKKTHYDFTKDR 1170
Query: 338 MISTFLCNLDPLKSVNAKNDDDFERIMFEISEKYEPRDIDNHNTFELALTKERRLNKQEQ 517
+ + N + +N E+ +++S EP+ + + NK ++
Sbjct: 1171 TVIECISN-------SLENIPHIEK-QYQLSLIIEPK------PRKKVVPNSNSNNKSQE 1216
Query: 518 KHADDMAN 541
K DD +
Sbjct: 1217 KSRDDQTD 1224
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,126,084
Number of Sequences: 369166
Number of extensions: 1717298
Number of successful extensions: 5247
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5042
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5246
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8390082510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)