Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_P09
(574 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q12965|MYO1E_HUMAN Myosin Ie (Myosin Ic) 72 8e-13
sp|Q63356|MYO1E_RAT Myosin Ie (Myosin heavy chain myr 3) 72 1e-12
sp|P80192|M3K9_HUMAN Mitogen-activated protein kinase kinas... 66 7e-11
sp|Q9JLQ0|CD2AP_MOUSE CD2-associated protein (Mesenchym-to-... 64 3e-10
sp|O89100|GRAP2_MOUSE GRB2-related adaptor protein 2 (GADS ... 64 3e-10
sp|P70248|MYO1F_MOUSE Myosin If 62 8e-10
sp|O00160|MYO1F_HUMAN Myosin If (Myosin-IE) 62 8e-10
sp|Q9Y5K6|CD2AP_HUMAN CD2-associated protein (Cas ligand wi... 62 1e-09
sp|O75791|GRAP2_HUMAN GRB2-related adaptor protein 2 (GADS ... 62 1e-09
sp|Q96B97|SH3K1_HUMAN SH3-domain kinase binding protein 1 (... 61 2e-09
>sp|Q12965|MYO1E_HUMAN Myosin Ie (Myosin Ic)
Length = 1109
Score = 72.4 bits (176), Expect = 8e-13
Identities = 34/82 (41%), Positives = 47/82 (57%)
Frame = +3
Query: 228 GVKRDDNAKDKVIXXXXXXXXXXXXXVNKVEALYDYEATDTDELTIRIGDIIELIKEDNE 407
GV+R ++ V + +ALY Y+A DTDEL+ DII++IKED
Sbjct: 1028 GVRRQTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDIIDIIKEDPS 1087
Query: 408 GWWTGKIGNRTGLMPSNYVKKI 473
GWWTG++ + GL P+NYV KI
Sbjct: 1088 GWWTGRLRGKPGLFPNNYVTKI 1109
>sp|Q63356|MYO1E_RAT Myosin Ie (Myosin heavy chain myr 3)
Length = 1107
Score = 72.0 bits (175), Expect = 1e-12
Identities = 33/82 (40%), Positives = 47/82 (57%)
Frame = +3
Query: 228 GVKRDDNAKDKVIXXXXXXXXXXXXXVNKVEALYDYEATDTDELTIRIGDIIELIKEDNE 407
GV+R ++ V + +ALY Y+A DTDEL+ D+I++IKED
Sbjct: 1026 GVRRQTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDVIDIIKEDPS 1085
Query: 408 GWWTGKIGNRTGLMPSNYVKKI 473
GWWTG++ + GL P+NYV KI
Sbjct: 1086 GWWTGRLRGKQGLFPNNYVTKI 1107
>sp|P80192|M3K9_HUMAN Mitogen-activated protein kinase kinase kinase 9 (Mixed lineage
kinase 1)
Length = 1104
Score = 65.9 bits (159), Expect = 7e-11
Identities = 26/53 (49%), Positives = 40/53 (75%), Gaps = 5/53 (9%)
Frame = +3
Query: 321 ALYDYEATDTDELTIRIGDIIELIKED-----NEGWWTGKIGNRTGLMPSNYV 464
A+++YEA DELT+R+GD++E++ +D +EGWWTG++ R G+ PSNYV
Sbjct: 59 AVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYV 111
>sp|Q9JLQ0|CD2AP_MOUSE CD2-associated protein (Mesenchym-to-epithelium transition protein
with SH3 domains 1) (METS-1)
Length = 637
Score = 63.9 bits (154), Expect = 3e-10
Identities = 24/52 (46%), Positives = 39/52 (75%)
Frame = +3
Query: 318 EALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYVKKI 473
+ L+DY + DEL + +GD+I++I+E EGWW+G + N+ GL PSN+VK++
Sbjct: 114 KVLFDYSPQNEDELELIVGDVIDVIEEVEEGWWSGTLNNKLGLFPSNFVKEL 165
Score = 57.0 bits (136), Expect = 3e-08
Identities = 25/50 (50%), Positives = 35/50 (70%), Gaps = 1/50 (2%)
Frame = +3
Query: 327 YDYEATDTDELTIRIGDIIELIKE-DNEGWWTGKIGNRTGLMPSNYVKKI 473
YDY+A DELTIR+G+II +K+ EGW G++ R G+ P N+VK+I
Sbjct: 8 YDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEI 57
Score = 49.3 bits (116), Expect = 7e-06
Identities = 24/52 (46%), Positives = 33/52 (63%), Gaps = 2/52 (3%)
Frame = +3
Query: 324 LYDYEATDTDELTIRIGDIIELI-KEDNE-GWWTGKIGNRTGLMPSNYVKKI 473
L+ Y T+ DELT R G+II LI KE E GWW G++ + G+ P N+ +I
Sbjct: 277 LFPYTGTNEDELTFREGEIIHLISKETGEAGWWKGELNGKEGVFPDNFAVQI 328
>sp|O89100|GRAP2_MOUSE GRB2-related adaptor protein 2 (GADS protein) (Growth factor
receptor binding protein) (GRBLG) (GRB-2-like protein)
(GRB2L) (Hematopoietic cell-associated adaptor protein
GrpL) (GRB-2-related monocytic adapter protein)
(Monocytic adapter) (MONA) (Adapter protein GRID)
Length = 322
Score = 63.5 bits (153), Expect = 3e-10
Identities = 25/53 (47%), Positives = 36/53 (67%)
Frame = +3
Query: 306 VNKVEALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYV 464
V ALYD+EA + DEL R G+++E++ N WWTG++ N+ GL P+NYV
Sbjct: 265 VRWARALYDFEALEEDELGFRSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYV 317
Score = 40.8 bits (94), Expect = 0.002
Identities = 16/48 (33%), Positives = 31/48 (64%)
Frame = +3
Query: 321 ALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYV 464
A +D+ A+ DEL+ R GDI++++ E W ++G++ G +P N++
Sbjct: 5 AKFDFMASGEDELSFRTGDILKILSNQEE-WLKAELGSQEGYVPKNFI 51
>sp|P70248|MYO1F_MOUSE Myosin If
Length = 1099
Score = 62.4 bits (150), Expect = 8e-10
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = +3
Query: 312 KVEALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYVKKI 473
+ ALY Y D DEL+ + ++IE++ ED+ GWW G++ + GL P NYV+KI
Sbjct: 1046 RCRALYQYIGQDVDELSFNVNEVIEILIEDSSGWWKGRLHGQEGLFPGNYVEKI 1099
>sp|O00160|MYO1F_HUMAN Myosin If (Myosin-IE)
Length = 1098
Score = 62.4 bits (150), Expect = 8e-10
Identities = 24/54 (44%), Positives = 35/54 (64%)
Frame = +3
Query: 312 KVEALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYVKKI 473
+ ALY Y D DEL+ + ++IE++ ED GWW G++ + GL P NYV+KI
Sbjct: 1045 RCRALYQYVGQDVDELSFNVNEVIEILMEDPSGWWKGRLHGQEGLFPGNYVEKI 1098
>sp|Q9Y5K6|CD2AP_HUMAN CD2-associated protein (Cas ligand with multiple SH3 domains)
(Adapter protein CMS)
Length = 639
Score = 62.0 bits (149), Expect = 1e-09
Identities = 25/62 (40%), Positives = 43/62 (69%)
Frame = +3
Query: 318 EALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYVKKI*FNNFFYT 497
+ L++Y + DEL +++GDII++ +E EGWW+G + N+ GL PSN+VK++ + T
Sbjct: 114 KVLFEYIPQNEDELELKVGDIIDINEEVEEGWWSGTLNNKLGLFPSNFVKELEVTDDGET 173
Query: 498 HK 503
H+
Sbjct: 174 HE 175
Score = 57.0 bits (136), Expect = 3e-08
Identities = 25/50 (50%), Positives = 35/50 (70%), Gaps = 1/50 (2%)
Frame = +3
Query: 327 YDYEATDTDELTIRIGDIIELIKE-DNEGWWTGKIGNRTGLMPSNYVKKI 473
YDY+A DELTIR+G+II +K+ EGW G++ R G+ P N+VK+I
Sbjct: 8 YDYDAVHDDELTIRVGEIIRNVKKLQEEGWLEGELNGRRGMFPDNFVKEI 57
Score = 50.1 bits (118), Expect = 4e-06
Identities = 24/52 (46%), Positives = 34/52 (65%), Gaps = 2/52 (3%)
Frame = +3
Query: 324 LYDYEATDTDELTIRIGDIIELI-KEDNE-GWWTGKIGNRTGLMPSNYVKKI 473
L+ YE T+ DELT + G+II LI KE E GWW G++ + G+ P N+ +I
Sbjct: 277 LFAYEGTNEDELTFKEGEIIHLISKETGEAGWWRGELNGKEGVFPDNFAVQI 328
>sp|O75791|GRAP2_HUMAN GRB2-related adaptor protein 2 (GADS protein) (Growth factor
receptor binding protein) (GRBLG) (Grf40 adaptor
protein) (Grf-40) (GRB-2-like protein) (GRB2L) (GRBX)
(P38) (Hematopoietic cell-associated adaptor protein
GrpL) (Adapter protein GRID) (SH3-SH2-SH3 adaptor Mona)
Length = 330
Score = 61.6 bits (148), Expect = 1e-09
Identities = 24/53 (45%), Positives = 35/53 (66%)
Frame = +3
Query: 306 VNKVEALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYV 464
V ALYD+EA + DEL G+++E++ N WWTG++ N+ GL P+NYV
Sbjct: 273 VRWARALYDFEALEDDELGFHSGEVVEVLDSSNPSWWTGRLHNKLGLFPANYV 325
Score = 40.0 bits (92), Expect = 0.004
Identities = 14/48 (29%), Positives = 31/48 (64%)
Frame = +3
Query: 321 ALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYV 464
A +D+ A+ DEL+ GD+++++ E W+ ++G++ G +P N++
Sbjct: 5 AKFDFTASGEDELSFHTGDVLKILSNQEE-WFKAELGSQEGYVPKNFI 51
>sp|Q96B97|SH3K1_HUMAN SH3-domain kinase binding protein 1 (Cbl-interacting protein of 85
kDa) (Human Src-family kinase binding protein 1) (HSB-1)
(CD2 binding protein 3) (CD2BP3)
Length = 665
Score = 60.8 bits (146), Expect = 2e-09
Identities = 27/55 (49%), Positives = 40/55 (72%), Gaps = 2/55 (3%)
Frame = +3
Query: 315 VEAL--YDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYVKKI 473
VEA+ +DY+A DELTI +G+II I++++ GWW G+I R GL P N+V++I
Sbjct: 2 VEAIVEFDYQAQHDDELTISVGEIITNIRKEDGGWWEGQINGRRGLFPDNFVREI 56
Score = 58.9 bits (141), Expect = 9e-09
Identities = 21/54 (38%), Positives = 37/54 (68%)
Frame = +3
Query: 312 KVEALYDYEATDTDELTIRIGDIIELIKEDNEGWWTGKIGNRTGLMPSNYVKKI 473
+ + + Y + DEL +++GDIIE++ E EGWW G + +TG+ PSN++K++
Sbjct: 102 RCQVAFSYLPQNDDELELKVGDIIEVVGEVEEGWWEGVLNGKTGMFPSNFIKEL 155
Score = 56.2 bits (134), Expect = 6e-08
Identities = 24/52 (46%), Positives = 36/52 (69%), Gaps = 2/52 (3%)
Frame = +3
Query: 318 EALYDYEATDTDELTIRIGDIIELIKED--NEGWWTGKIGNRTGLMPSNYVK 467
+ ++ YEA + DELTI+ GDI+ LI +D + GWW G++ R G+ P N+VK
Sbjct: 273 KVIFPYEAQNDDELTIKEGDIVTLINKDCIDVGWWEGELNGRRGVFPDNFVK 324
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,004,864
Number of Sequences: 369166
Number of extensions: 844335
Number of successful extensions: 2888
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2659
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2796
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)