Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_O04
(799 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q16658|FSCN1_HUMAN Fascin (Singed-like protein) (55 kDa ... 169 6e-42
sp|Q24524|SING_DROME Singed protein 160 4e-39
sp|Q61553|FSC1_MOUSE Fascin (Singed-like protein) 157 3e-38
sp|Q91837|FASC_XENLA Fascin 152 1e-36
sp|O18728|FSCN2_BOVIN Fascin-2 (Retinal fascin) 135 2e-31
sp|O14926|FSCN2_HUMAN Fascin 2 (Retinal fascin) 131 2e-30
sp|Q05634|FASC_STRPU Fascin 96 1e-19
sp|Q6XYZ4|CBIO1_SPIKU Cobalt import ATP-binding protein cbiO 1 36 0.14
sp|P46340|YQGI_BACSU Probable ABC transporter permease prot... 32 1.5
sp|Q6G1W2|SYL_BARHE Leucyl-tRNA synthetase (Leucine--tRNA l... 32 1.5
>sp|Q16658|FSCN1_HUMAN Fascin (Singed-like protein) (55 kDa actin bundling protein) (p55)
Length = 493
Score = 169 bits (429), Expect = 6e-42
Identities = 106/249 (42%), Positives = 150/249 (60%), Gaps = 3/249 (1%)
Frame = +3
Query: 3 SARGRYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKS-KNK 179
+A R+L DG L + + Y LE R G F+D EG+YL G +GT+K+ K
Sbjct: 190 TADHRFLRHDGRLVARPEPATGYTLEFRSGKV---AFRDCEGRYLAPSGPSGTLKAGKAT 246
Query: 180 VVGKDEIFTLEISYPQVGLRAHNGKYASIKQGVDLYANQTDAEEKEIFQLEYQNSSKKWK 359
VGKDE+F LE S QV L+A N + S +QG+DL ANQ + ++E FQLE +KK
Sbjct: 247 KVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCA 306
Query: 360 IRTCDGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSKKLGLMS 539
RT GKYWT+T+ G+++T+ +N SC F+ I ++ ++ASN +V+SKK G ++
Sbjct: 307 FRTHTGKYWTLTA-TGGVQSTASSKNASCYFD-IEWRDRRITLRASNGKFVTSKKNGQLA 364
Query: 540 ATGENAEDPESLFQAILLNRPMIIFKSEFGLVGTR--NGRIECNLSSYEAFQLESVLDGN 713
A+ E A D E LF L+NRP+I+F+ E G +G R G ++ N SSY+ FQLE DG
Sbjct: 365 ASVETAGDSE-LFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLE-FNDGA 422
Query: 714 YILKASGNK 740
Y +K S K
Sbjct: 423 YNIKDSTGK 431
Score = 49.3 bits (116), Expect = 1e-05
Identities = 57/217 (26%), Positives = 85/217 (39%), Gaps = 38/217 (17%)
Frame = +3
Query: 45 DKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKSKNKVVGKDEIFTLEISYP 224
D+ TD + LE+ T F+ + GKY TL +TG V+S F +E
Sbjct: 286 DEETDQETFQLEIDRD-TKKCAFRTHTGKYWTLT-ATGGVQSTASSKNASCYFDIEWRDR 343
Query: 225 QVGLRAHNGKYASIKQGVDLYANQTDAEEKE----------------------------- 317
++ LRA NGK+ + K+ L A+ A + E
Sbjct: 344 RITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGT 403
Query: 318 ---------IFQLEYQNSSKKWKIRTCDGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLP 470
+FQLE+ + + + I+ GKYWTV S +S D FE
Sbjct: 404 LDANRSSYDVFQLEFNDGA--YNIKDSTGKYWTVGS--DSAVTSSGDTPVDFFFEFCDY- 458
Query: 471 SGKVMIKASNNMYVSSKKLGLMSATGENAEDPESLFQ 581
KV IK Y+ G++ A+ E DP SL++
Sbjct: 459 -NKVAIKVGGR-YLKGDHAGVLKASAETV-DPASLWE 492
>sp|Q24524|SING_DROME Singed protein
Length = 512
Score = 160 bits (405), Expect = 4e-39
Identities = 94/250 (37%), Positives = 142/250 (56%), Gaps = 4/250 (1%)
Frame = +3
Query: 3 SARGRYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKSKNKV 182
+ +YLN +G L ++ L+ E GG +D +G+YL+ IGS +KS++
Sbjct: 205 TCNNKYLNANGKLQVVCNEDCLFSAEYHGGH---LALRDRQGQYLSPIGSKAVLKSRSSS 261
Query: 183 VGKDEIFTLEISYPQVGLRAH-NGKYASIKQGVDLYANQTDAEEKEIFQLEYQNSSKKWK 359
V +DE+F+LE S PQ A N +Y S+KQGVD+ ANQ + E E FQLEY S+ +W
Sbjct: 262 VTRDELFSLEDSLPQASFIAGLNLRYVSVKQGVDVTANQDEVGENETFQLEYDWSAHRWA 321
Query: 360 IRTCDGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSKKLGLMS 539
+RT +YW + S GI+AT R LFE+I G + +A+N ++++K+ G +
Sbjct: 322 LRTTQDRYWCL-SAGGGIQATGNRRCADALFELIWHGDGSLSFRANNGKFLATKRSGHLF 380
Query: 540 ATGENAEDPESLFQAILLNRPMIIFKSEFGLVGTR---NGRIECNLSSYEAFQLESVLDG 710
AT E+ E+ + F L+NRP+++ K E G VG R N ++ECN ++YE +E G
Sbjct: 381 ATSESIEE-IAKFYFYLINRPILVLKCEQGFVGYRTPGNLKLECNKATYETILVERAQKG 439
Query: 711 NYILKASGNK 740
LKA K
Sbjct: 440 LVHLKAHSGK 449
>sp|Q61553|FSC1_MOUSE Fascin (Singed-like protein)
Length = 493
Score = 157 bits (397), Expect = 3e-38
Identities = 100/245 (40%), Positives = 145/245 (59%), Gaps = 3/245 (1%)
Frame = +3
Query: 15 RYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKS-KNKVVGK 191
R+L DG L + + + LE R G F+D EG+YL G +GT+K+ K VGK
Sbjct: 194 RFLRHDGRLVARPEPATGFTLEFRSGKV---AFRDCEGRYLAPSGPSGTLKAGKATKVGK 250
Query: 192 DEIFTLEISYPQVGLRAHNGKYASIKQGVDLYANQTDAEEKEIFQLEYQNSSKKWKIRTC 371
DE+F LE S +V L+A N S +QG+DL ANQ + ++E FQLE ++K RT
Sbjct: 251 DELFALEQSCAEVVLQAANEGNVSTRQGMDLSANQDEETDQETFQLEIDRDTRKCAFRTH 310
Query: 372 DGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSKKLGLMSATGE 551
GKYWT+T+ G+++T+ +N SC F+ I ++ ++ASN +V++KK G ++A+ E
Sbjct: 311 TGKYWTLTA-TGGVQSTASTKNASCYFD-IEWCDRRITLRASNGKFVTAKKNGHVAASVE 368
Query: 552 NAEDPESLFQAILLNRPMIIFKSEFGLVGTR--NGRIECNLSSYEAFQLESVLDGNYILK 725
A D E LF L+NRP+I F+ E G + R G ++ N SSY+ FQLE DG Y +K
Sbjct: 369 TAGDSE-LFLMKLINRPIIAFRGEHGFIACRKVTGTLDANRSSYDVFQLE-FNDGAYNIK 426
Query: 726 ASGNK 740
S K
Sbjct: 427 DSTGK 431
Score = 49.7 bits (117), Expect = 9e-06
Identities = 45/159 (28%), Positives = 78/159 (49%), Gaps = 1/159 (0%)
Frame = +3
Query: 108 TFKDNEGKYLTLIGSTGTVKSKNKVVGKDEIFTLE-ISYPQVGLRAHNGKYASIKQGVDL 284
T + + GK++T G V + + G E+F ++ I+ P + R +G A K L
Sbjct: 346 TLRASNGKFVTA-KKNGHVAASVETAGDSELFLMKLINRPIIAFRGEHGFIACRKVTGTL 404
Query: 285 YANQTDAEEKEIFQLEYQNSSKKWKIRTCDGKYWTVTSVNSGIKATSEDRNDSCLFEIIP 464
AN++ + +FQLE+ + + + I+ GKYWTV S +S + ++S+ D F +
Sbjct: 405 DANRSSYD---VFQLEFNDGA--YNIKDSTGKYWTVGS-DSSVTSSSDTPVD---FFLEF 455
Query: 465 LPSGKVMIKASNNMYVSSKKLGLMSATGENAEDPESLFQ 581
KV +K Y+ G++ A E DP SL++
Sbjct: 456 CDYNKVALKVGGR-YLKGDHAGVLKACAETI-DPASLWE 492
>sp|Q91837|FASC_XENLA Fascin
Length = 483
Score = 152 bits (384), Expect = 1e-36
Identities = 100/256 (39%), Positives = 145/256 (56%), Gaps = 4/256 (1%)
Frame = +3
Query: 15 RYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKS-KNKVVGK 191
R L DGSL +K ++ Y L++ G F+ ++G+YLT G +GT+KS KN G+
Sbjct: 185 RLLASDGSLREKPGPDTAYTLDISAGKV---AFRGSDGRYLTSSGPSGTLKSGKNSKAGR 241
Query: 192 DEIFTLEISYPQVGLRAHNGKYASIKQGVDLYANQTDAEEKEIFQLEYQNSSKKWKIRTC 371
DE+F LE S PQV L A NG + + ANQ + ++E FQLE +K RT
Sbjct: 242 DELFVLERSCPQVVLTAGNGGTCLQGKVLIFSANQDEESDQETFQLEINKDTKMCAFRTH 301
Query: 372 DGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSKKLGLMSATGE 551
GKYWT++S N GI+A++ N SC FE I ++ +K N YV++KK G +SA+ +
Sbjct: 302 TGKYWTLSS-NGGIQASASTLNSSCYFE-IDWCDRRITLKGVNGKYVTAKKNGQLSASVD 359
Query: 552 NAEDPESLFQAILLNRPMIIFKSEFGLVGTR--NGRIECNLSSYEAFQLESVLDGNYILK 725
+ E LF L+NRP+I+ + E G +G R G ++ N S Y+ F+LE DG Y LK
Sbjct: 360 TPGETE-LFLMKLINRPLIVLRGEHGFIGCRKMTGTLDSNRSIYDVFELE-FNDGAYSLK 417
Query: 726 -ASGNKMPFGKYLMIT 770
A+G G + +T
Sbjct: 418 DATGKYWTVGSDMSVT 433
>sp|O18728|FSCN2_BOVIN Fascin-2 (Retinal fascin)
Length = 492
Score = 135 bits (339), Expect = 2e-31
Identities = 88/247 (35%), Positives = 137/247 (55%), Gaps = 3/247 (1%)
Frame = +3
Query: 3 SARGRYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKS-KNK 179
S RYL DG L + + Y LE + G FKD +G YL +G GT+++ +N
Sbjct: 188 SCDSRYLRSDGRLVWEPEARARYTLEFKAGK---LAFKDCDGHYLAPVGPAGTLRAGRNT 244
Query: 180 VVGKDEIFTLEISYPQVGLRAHNGKYASIKQGVDLYANQTDAEEKEIFQLEYQNSSKKWK 359
GKDE+F LE S+PQV L A N +Y S++QGV++ ANQ + + E F ++ +KK
Sbjct: 245 RPGKDELFDLEESHPQVVLVAANHRYVSVRQGVNVSANQDEELDHETFLMQIDQETKKCT 304
Query: 360 IRTCDGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSKKLGLMS 539
+ G YWT+ + + GI+AT+ +++ +FE + +V +KASN YV KK G ++
Sbjct: 305 FYSSTGGYWTLVT-HGGIQATATQVSENTMFE-MEWRGRRVALKASNGRYVCMKKNGQLA 362
Query: 540 ATGENAEDPESLFQAILLNRPMIIFKSEFGLVGTRNG--RIECNLSSYEAFQLESVLDGN 713
A + + E F L+NRP+++ + G V R G +++ N S Y+ F L S DG
Sbjct: 363 AISDFVGEDEE-FTLKLINRPILVLRGLDGFVCHRRGSNQLDTNRSVYDVFHL-SFSDGA 420
Query: 714 YILKASG 734
Y ++ G
Sbjct: 421 YQIRGRG 427
>sp|O14926|FSCN2_HUMAN Fascin 2 (Retinal fascin)
Length = 492
Score = 131 bits (329), Expect = 2e-30
Identities = 88/244 (36%), Positives = 132/244 (54%), Gaps = 3/244 (1%)
Frame = +3
Query: 3 SARGRYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKS-KNK 179
S RYL DG L + + Y LE + G FKD +G YL +G GT+K+ +N
Sbjct: 188 SCDSRYLRSDGRLVWEPEPRACYTLEFKAGK---LAFKDCDGHYLAPVGPAGTLKAGRNT 244
Query: 180 VVGKDEIFTLEISYPQVGLRAHNGKYASIKQGVDLYANQTDAEEKEIFQLEYQNSSKKWK 359
GKDE+F LE S+PQV L A N +Y S++QGV++ ANQ D + E F ++ +KK
Sbjct: 245 RPGKDELFDLEESHPQVVLVAANHRYVSVRQGVNVSANQDDELDHETFLMQIDQETKKCT 304
Query: 360 IRTCDGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSKKLGLMS 539
+ G YWT+ + + GI AT+ + + +FE + +V +KASN YV KK G ++
Sbjct: 305 FYSSTGGYWTLVT-HGGIHATATQVSANTMFE-MEWRGRRVALKASNGRYVCMKKNGQLA 362
Query: 540 ATGENAEDPESLFQAILLNRPMIIFKSEFGLVGTRNG--RIECNLSSYEAFQLESVLDGN 713
A + E F L+NRP+++ + G V G +++ N S Y+ F L S DG
Sbjct: 363 AISDFVGKDEE-FTLKLINRPILVLRGLDGFVCHHRGSNQLDTNRSVYDVFHL-SFSDGA 420
Query: 714 YILK 725
Y ++
Sbjct: 421 YRIR 424
>sp|Q05634|FASC_STRPU Fascin
Length = 496
Score = 95.9 bits (237), Expect = 1e-19
Identities = 75/255 (29%), Positives = 117/255 (45%), Gaps = 12/255 (4%)
Frame = +3
Query: 12 GRYLNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKSKNKVVGK 191
G+++ DG L+ + + + L G +DN G++L + T +KS + K
Sbjct: 192 GKFVQTDGQLAGTANEQTQFTLIFTSGH---LVLRDNNGRHLGVDSGTRVLKSSKPGLTK 248
Query: 192 DEIFTLEISYPQVGLRAHNGKYASIKQGVDLYAN---QTDAEEKEIFQLEYQNSSKKWKI 362
F LE S PQ G GKYAS+KQG D+ D E+ E FQLE+ + K+ I
Sbjct: 249 ANYFILEDSCPQ-GAFEFGGKYASLKQGEDVSFKLLVDEDIEDTETFQLEFVETD-KYAI 306
Query: 363 RTC-------DGKYWTVTSVNSGIKATSEDRNDSCLFEIIPLPSGKVMIKASNNMYVSSK 521
R C D K+W + S+D+ D C F + + ++A YVS +
Sbjct: 307 RVCDPKKNSRDAKFWKTVAAGIQANGNSKDQTD-CQFS-VEYNGNDMHVRAPGGKYVSVR 364
Query: 522 KLGLMSATGENAEDPESLFQAILLNRPMIIFKSEFGLVGTRNGRIE--CNLSSYEAFQLE 695
G + +D F LLNRP ++ K G VG + G+ E CN S+++ F +
Sbjct: 365 DNGHLF-----LQDSPKDFIFRLLNRPKLVLKCPHGFVGMKEGKAEVACNRSNFDVFTV- 418
Query: 696 SVLDGNYILKASGNK 740
+ +G Y ++ S K
Sbjct: 419 TYKEGGYTIQDSCGK 433
>sp|Q6XYZ4|CBIO1_SPIKU Cobalt import ATP-binding protein cbiO 1
Length = 288
Score = 35.8 bits (81), Expect = 0.14
Identities = 25/74 (33%), Positives = 38/74 (51%), Gaps = 9/74 (12%)
Frame = +3
Query: 21 LNKDGSLSDKITDNSLYGLELRGGATPGFTFKDNEGKYLTLIGSTGTVKSK-NKVV---- 185
L K +S K+TD E A G +F+ N G+Y+T+IG G+ KS +K++
Sbjct: 12 LEKLNYISLKLTDVEFRYRENHPNAVDGVSFEINHGEYVTIIGHNGSGKSTISKIIIGVL 71
Query: 186 ----GKDEIFTLEI 215
GK E+F E+
Sbjct: 72 RPQKGKIEVFGNEV 85
>sp|P46340|YQGI_BACSU Probable ABC transporter permease protein yqgI
Length = 294
Score = 32.3 bits (72), Expect = 1.5
Identities = 22/87 (25%), Positives = 42/87 (48%), Gaps = 9/87 (10%)
Frame = -1
Query: 610 IIIGRFNNMAWKRLSG-SSAFSPVADINPNFFDETYILLEALIITLPLGKG--------I 458
III + ++++ ++ SSA + I F+ YIL ++IT+PLG G
Sbjct: 32 IIINGVSQLSFQFITTKSSAIAAGGGIRDQLFNSFYILFITMLITIPLGVGGGVFMAEYA 91
Query: 457 ISNKQESFLSSLVALIPLLTEVTVQYF 377
+NK F+ + + ++ L + + F
Sbjct: 92 PNNKVTDFIRTCIEVLSSLPSIVIGMF 118
>sp|Q6G1W2|SYL_BARHE Leucyl-tRNA synthetase (Leucine--tRNA ligase) (LeuRS)
Length = 880
Score = 32.3 bits (72), Expect = 1.5
Identities = 18/81 (22%), Positives = 34/81 (41%), Gaps = 1/81 (1%)
Frame = +3
Query: 327 LEYQNSSKKWKIRTCDGKYWTVTSVNSGIKATSEDRNDS-CLFEIIPLPSGKVMIKASNN 503
+E+ N +++ R C+ K+ + + EDR + + E+ P PSG++ + N
Sbjct: 3 IEHYNLGERYNPRACERKWQAIWDEKKTFQTVQEDRREKYYVLEMFPYPSGRIHMGHVRN 62
Query: 504 MYVSSKKLGLMSATGENAEDP 566
+ A G N P
Sbjct: 63 YAMGDVVARYKRAKGFNVLHP 83
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,935,185
Number of Sequences: 369166
Number of extensions: 1850894
Number of successful extensions: 5651
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5437
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5619
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7522142940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)