Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_N19
(579 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 92 9e-19
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 90 5e-18
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 88 1e-17
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 84 2e-16
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 83 4e-16
sp|P42662|ASTL_COTJA Astacin-like metalloendopeptidase 83 6e-16
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 82 1e-15
sp|Q16819|MEP1A_HUMAN Meprin A alpha-subunit precursor (End... 80 4e-15
sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precurs... 80 4e-15
sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor... 80 4e-15
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 92.0 bits (227), Expect = 9e-19
Identities = 57/156 (36%), Positives = 86/156 (55%), Gaps = 6/156 (3%)
Frame = +3
Query: 96 CVKFSESSASHYIEVQSL--DQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHAL 269
CVKF S + + + D+GCYSL+G TGG KQP++L + GC+ GT+ HE +HA+
Sbjct: 137 CVKFVARDPSKHHDYLWIHPDEGCYSLVGKTGG-KQPVSLDS-GCI-QVGTIVHELMHAV 193
Query: 270 GFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCD---GCEVDGPYETHSVMHYPSYGFAC 440
GF H R+DRD++I+V W ++ QF K + +D PY+ S+MHY Y F+
Sbjct: 194 GFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSG 253
Query: 441 VPGENVVFKRDGGLIDYNHVT-SRNDLDMVRRFYQC 545
+ +V K+ G V S D+ + + Y C
Sbjct: 254 SGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 89.7 bits (221), Expect = 5e-18
Identities = 54/160 (33%), Positives = 81/160 (50%), Gaps = 10/160 (6%)
Frame = +3
Query: 96 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 272
C++F + + +I V S GCYS LG GG +Q L++ GCMYS G ++HE HALG
Sbjct: 120 CIRFVRRTNEYDFISVVS-KTGCYSELGRKGG-QQELSINRGGCMYS-GIIQHELNHALG 176
Query: 273 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHYPSYGFAC---- 440
F H R DRD+++ + W+ I+ + F K D ++ PY+ S+MHY F+
Sbjct: 177 FQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 236
Query: 441 -----VPGENVVFKRDGGLIDYNHVTSRNDLDMVRRFYQC 545
+P NV + G+ SR D+ + Y C
Sbjct: 237 DSITPIPNPNVPIGQRNGM-------SRWDITRINVLYNC 269
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 88.2 bits (217), Expect = 1e-17
Identities = 55/160 (34%), Positives = 80/160 (50%), Gaps = 10/160 (6%)
Frame = +3
Query: 96 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 272
C++F + + +I V S + GCYS LG GG +Q L+L GCMYS G ++HE HALG
Sbjct: 129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGG-QQELSLNRGGCMYS-GIIQHELNHALG 185
Query: 273 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDGPYETHSVMHYPSYGFAC---- 440
F H R DRD+++ + W I+ + F K D ++ PY+ S+MHY F+
Sbjct: 186 FQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 245
Query: 441 -----VPGENVVFKRDGGLIDYNHVTSRNDLDMVRRFYQC 545
+P NV + G+ SR D+ Y C
Sbjct: 246 DSITPIPNPNVPIGQRNGM-------SRWDITRSNVLYNC 278
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 84.3 bits (207), Expect = 2e-16
Identities = 52/174 (29%), Positives = 82/174 (47%), Gaps = 2/174 (1%)
Frame = +3
Query: 30 FPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLN 206
+ +D+K +R + E + C+ F ++ Y+ ++ GC S++GY G KQ +
Sbjct: 103 YESDEKETIRNAMKEFAEK--TCIHFVPRNNERAYLSLEPRF-GCKSMMGYVGD-KQVVV 158
Query: 207 LQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVD 386
LQ GC+ ++HE +HALGF H H R DRD H+ + W+ I+ F K D +
Sbjct: 159 LQRFGCI-KHAVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLG 217
Query: 387 GPYETHSVMHYPSYGFACVPGENVV-FKRDGGLIDYNHVTSRNDLDMVRRFYQC 545
PY+ S+MHY F E + I S D+ V + Y+C
Sbjct: 218 TPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 83.2 bits (204), Expect = 4e-16
Identities = 48/144 (33%), Positives = 81/144 (56%), Gaps = 2/144 (1%)
Frame = +3
Query: 12 FSFAPAFPNDKKGIVRECLVELQKDLGNCVKF-SESSASHYIEVQSLDQGCYSLLGYTGG 188
++FA D+ I+ + EL++ C++F ++ S Y+E+ + GC+S +G G
Sbjct: 66 YTFAGVSGADQSAILSG-MQELEEK--TCIRFVPRTTESDYVEIFTSGSGCWSYVGRISG 122
Query: 189 PKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCSQF-VK 365
+Q ++LQ GC+Y GT+ HE +HA+GF H H R DRDN++ + + + S S F +
Sbjct: 123 AQQ-VSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSMTSNFDID 180
Query: 366 CDGCEVDGPYETHSVMHYPSYGFA 437
V Y+ +S+MHY Y F+
Sbjct: 181 TYSRYVGEDYQYYSIMHYGKYSFS 204
>sp|P42662|ASTL_COTJA Astacin-like metalloendopeptidase
Length = 310
Score = 82.8 bits (203), Expect = 6e-16
Identities = 49/154 (31%), Positives = 78/154 (50%), Gaps = 4/154 (2%)
Frame = +3
Query: 96 CVKFSESSASH-YIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALG 272
C+ F + Y+ ++S D GC+S G GG Q +++ GCM+ KG ++HE HALG
Sbjct: 35 CINFVKRKTERDYLIIRSAD-GCWSNYGKVGGG-QTVSVMKGGCMW-KGIIQHELDHALG 91
Query: 273 FMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDG-PYETHSVMHYPSYGFACVPG 449
F+H H R DRD ++ + W+ I + F K + G PY+ SVMHY + F G
Sbjct: 92 FLHEHSRSDRDKYVKIMWEYISPACRPDFRKFENSNNLGLPYDYSSVMHYGPHTFTNTTG 151
Query: 450 ENVVFKRDGGLIDYNHVTSRNDLDM--VRRFYQC 545
+ + G + ++LD+ + + Y C
Sbjct: 152 KATIVPVPDGSVHIGQRLGLSNLDVAKINKLYNC 185
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 82.0 bits (201), Expect = 1e-15
Identities = 50/155 (32%), Positives = 82/155 (52%), Gaps = 5/155 (3%)
Frame = +3
Query: 96 CVKF--SESSASHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHAL 269
C+++ E++ +Y+ + D+GCYS++G GG KQ L+L + GC+ KG + HE +HA+
Sbjct: 156 CIRWVPKEAADVNYVHIYP-DRGCYSMVGKMGG-KQSLSLGS-GCI-QKGIILHELMHAV 211
Query: 270 GFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCEVDG---PYETHSVMHYPSYGFAC 440
GF H R DRD+HI + W+ I QF K + Y+ S+MHY + F+
Sbjct: 212 GFFHEQSRTDRDDHITIMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFSR 271
Query: 441 VPGENVVFKRDGGLIDYNHVTSRNDLDMVRRFYQC 545
++ K++G I + S+ D + Y C
Sbjct: 272 NGQPTMIPKKNGATIGQRNGFSKVDKFKINTLYGC 306
>sp|Q16819|MEP1A_HUMAN Meprin A alpha-subunit precursor (Endopeptidase-2)
(N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase
alpha subunit) (PABA peptide hydrolase) (PPH alpha)
Length = 746
Score = 80.1 bits (196), Expect = 4e-15
Identities = 55/162 (33%), Positives = 81/162 (50%), Gaps = 9/162 (5%)
Frame = +3
Query: 87 LGNCVKFSE-SSASHYIEVQSLDQGCYSLLG--YTGGPKQPLNLQNPGCMYSKGTVKHEF 257
L +CV F S YI Q D GC+S +G + G Q +++ GC Y K ++HE
Sbjct: 104 LKSCVDFKPYEGESSYIIFQQFD-GCWSEVGDQHVG---QNISI-GQGCAY-KAIIEHEI 157
Query: 258 IHALGFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDG---CEVDGPYETHSVMHYPSY 428
+HALGF H R DRD+++N+ WD+IL+ + F D +++ PY+ S+MHY +
Sbjct: 158 LHALGFYHEQSRTDRDDYVNIWWDQILSGYQHNFDTYDDSLITDLNTPYDYESLMHYQPF 217
Query: 429 GF---ACVPGENVVFKRDGGLIDYNHVTSRNDLDMVRRFYQC 545
F A VP +I S DL+ + R Y C
Sbjct: 218 SFNKNASVPTITAKIPEFNSIIGQRLDFSAIDLERLNRMYNC 259
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
Length = 503
Score = 80.1 bits (196), Expect = 4e-15
Identities = 56/187 (29%), Positives = 87/187 (46%), Gaps = 6/187 (3%)
Frame = +3
Query: 3 QVLFSFAPAFPNDKKGIVRECLVELQKDLGNCVKFSESSASH--YIEVQ-SLDQGCYSLL 173
QV + ND++ + + E + CV+F + YI V+ ++ GC S +
Sbjct: 129 QVPYMLEEGMTNDQRTAIAQAFDEYKTK--TCVRFVPKTDDDFDYIYVKRNVAFGCSSYV 186
Query: 174 GYTGGPKQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHINVKWDRILTSHCS 353
G GG Q ++L+ C +SKG + HE +HALGF H H R DRD+ +++ D I
Sbjct: 187 GRAGG-NQTVSLEVDKC-FSKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIRPGMMR 244
Query: 354 QFVKCDGCEVDG---PYETHSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDM 524
F K +D PY+ SVMHY F+ ++ K + + + S D
Sbjct: 245 NFEKYPRKIIDSLGMPYDYESVMHYHKLAFSRNGKPTIIPKDNEADVGQRYKLSEMDSKK 304
Query: 525 VRRFYQC 545
V + YQC
Sbjct: 305 VNKLYQC 311
>sp|Q18439|NAS8_CAEEL Zinc metalloproteinase nas-8 precursor (Nematode astacin 8)
Length = 403
Score = 80.1 bits (196), Expect = 4e-15
Identities = 58/166 (34%), Positives = 85/166 (51%), Gaps = 11/166 (6%)
Frame = +3
Query: 96 CVKFSESSA--SHYIEVQSLDQGCYSLLGYTGGPKQPLNLQNPGCMYSKGTVKHEFIHAL 269
CV+F +A + Y+ + +D GCYS +G GG +Q L+L N GC+ T HE +H++
Sbjct: 154 CVRFVPRTAVDNDYLYIGKID-GCYSDVGRAGG-RQELSLDN-GCL-QYDTAIHELMHSV 209
Query: 270 GFMHTHMRKDRDNHINVKWDRILTSHCSQFVKCDGCE---VDGPYETHSVMHYPSYGFAC 440
GF H H R DRD HI + W I QF K D E Y+ +S+MHY S F+
Sbjct: 210 GFYHEHERWDRDEHITILWHNIDREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFSK 269
Query: 441 VPGENVVFKRD------GGLIDYNHVTSRNDLDMVRRFYQC*YLKI 560
E +V K+ G ID++ + D+ + YQC +K+
Sbjct: 270 NGFETMVAKQSEMTAVIGAAIDFSPI----DILKMNLMYQCSDVKL 311
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,088,545
Number of Sequences: 369166
Number of extensions: 1656041
Number of successful extensions: 3728
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3569
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3678
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4259329035
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)