Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_N03
(744 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precurs... 100 7e-21
sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatch... 89 9e-18
sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-mole... 87 4e-17
sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (... 84 3e-16
sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precurs... 83 7e-16
sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (... 83 9e-16
sp|Q61847|MEP1B_MOUSE Meprin A beta-subunit precursor (Endo... 81 3e-15
sp|P28826|MEP1B_RAT Meprin A beta-subunit precursor (Endope... 80 7e-15
sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor... 79 1e-14
sp|Q6HA08|ASTL_HUMAN Astacin-like metalloendopeptidase prec... 76 8e-14
>sp|Q19269|NAS14_CAEEL Zinc metalloproteinase nas-14 precursor (Nematode astacin 14)
Length = 503
Score = 99.8 bits (247), Expect = 7e-21
Identities = 62/194 (31%), Positives = 94/194 (48%), Gaps = 6/194 (3%)
Frame = -1
Query: 594 RIWPRGQVLFAFAQNFPYDKQNLVRQCLGELQSDLGGCIQFFESTEGPFIGVHAQDKGCF 415
++WP GQV + + D++ + Q E ++ C++F T+ F ++ + F
Sbjct: 123 KLWPEGQVPYMLEEGMTNDQRTAIAQAFDEYKTKT--CVRFVPKTDDDFDYIYVKRNVAF 180
Query: 414 AMIGFTGEYG--QFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRRDRNNYIMINWDNIK 241
+ G G Q ++L+ C SKG I HE +HALGF H H R DR++++ IN DNI+
Sbjct: 181 GCSSYVGRAGGNQTVSLEVDKCF-SKGIIAHELMHALGFFHEHSRTDRDDFVDINEDNIR 239
Query: 240 SDQCFNFEICNGCILDG---DYETRSVMHYSSDAFGCEQGMNTMIQTSGEL-IDYNHNTT 73
NFE I+D Y+ SVMHY AF G T+I E + + +
Sbjct: 240 PGMMRNFEKYPRKIIDSLGMPYDYESVMHYHKLAFS-RNGKPTIIPKDNEADVGQRYKLS 298
Query: 72 PNDLNMIRNLYQCG 31
D + LYQCG
Sbjct: 299 EMDSKKVNKLYQCG 312
>sp|P31579|LCE_ORYLA Low choriolytic enzyme precursor (Hatching enzyme zinc-protease LCE
subunit) (Choriolysin L)
Length = 271
Score = 89.4 bits (220), Expect = 9e-18
Identities = 68/234 (29%), Positives = 111/234 (47%), Gaps = 11/234 (4%)
Frame = -1
Query: 702 SKLLKRSSNN----VLVGDMLLTKADIAVISGYKHHQGDIRIWPRG-----QVLFAFAQN 550
S ++ R +NN +L GD++L K A+ D WP+ +V + + N
Sbjct: 46 SSIIFRMNNNSMEELLEGDLVLPKTRNAMKC---FGAPDSCRWPKSSNGIVKVPYVVSDN 102
Query: 549 FPYDKQNLVRQCLGELQSDLGGCIQFF-ESTEGPFIGVHAQDKGCFAMIGFTGEYGQFLN 373
+ D++ +R + E CI F + E ++ + + GC +M+G+ G+ Q +
Sbjct: 103 YESDEKETIRNAMKEFAEKT--CIHFVPRNNERAYLSLEPRF-GCKSMMGYVGDK-QVVV 158
Query: 372 LQNPWCMNSKGTIKHEFIHALGFMHTHMRRDRNNYIMINWDNIKSDQCFNFEICNGCILD 193
LQ C+ I+HE +HALGF H H R DR+ ++ INW+NI D NF+ + L
Sbjct: 159 LQRFGCIKH-AVIQHELLHALGFYHEHTRSDRDQHVKINWENIIKDFTHNFDKNDTDNLG 217
Query: 192 GDYETRSVMHYSSDAFGCEQGMN-TMIQTSGELIDYNHNTTPNDLNMIRNLYQC 34
Y+ S+MHY AFG ++ T I I + D+ + LY+C
Sbjct: 218 TPYDYGSIMHYGRTAFGKDRKETITPIPNPKAAIGQTERMSDIDILRVNKLYKC 271
>sp|P07584|ASTA_ASTFL Astacin precursor (Crayfish small-molecule proteinase)
Length = 251
Score = 87.4 bits (215), Expect = 4e-17
Identities = 62/195 (31%), Positives = 93/195 (47%), Gaps = 7/195 (3%)
Frame = -1
Query: 603 GDIRIWPRGQVLFAFAQNFPYDKQNLVRQCLGELQSDLGGCIQFF-ESTEGPFIGVHAQD 427
GD +W G + + FA D Q+ + + EL+ CI+F +TE ++ +
Sbjct: 54 GDEYLWSGGVIPYTFAGVSGAD-QSAILSGMQELEEKT--CIRFVPRTTESDYVEIFTSG 110
Query: 426 KGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRRDRNNYIMINWDN 247
GC++ +G Q ++LQ C+ GTI HE +HA+GF H H R DR+NY+ IN+ N
Sbjct: 111 SGCWSYVGRISG-AQQVSLQANGCVYH-GTIIHELMHAIGFYHEHTRMDRDNYVTINYQN 168
Query: 246 IKSDQCFNFEICNGCILDG-DYETRSVMHYSSDAFGCEQGMNTMIQTSGELIDYNH---- 82
+ NF+I G DY+ S+MHY +F + G+ I ID
Sbjct: 169 VDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNGIDLTDPYDK 228
Query: 81 -NTTPNDLNMIRNLY 40
+ D N I NLY
Sbjct: 229 AHMLQTDANQINNLY 243
>sp|P31580|HCE23_ORYLA High choriolytic enzyme 1 precursor (Hatching enzyme zinc-protease
HCE 1 subunit) (Choriolysin H 1)
Length = 270
Score = 84.3 bits (207), Expect = 3e-16
Identities = 56/153 (36%), Positives = 78/153 (50%), Gaps = 3/153 (1%)
Frame = -1
Query: 483 CIQFFEST-EGPFIGVHAQDKGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHALG 307
CI+F T E FI V ++ GC++ +G G Q L++ CM S G I+HE HALG
Sbjct: 120 CIRFVRRTNEYDFISVVSKT-GCYSELGRKGGQ-QELSINRGGCMYS-GIIQHELNHALG 176
Query: 306 FMHTHMRRDRNNYIMINWDNIKSDQCFNFEICNGCILDGDYETRSVMHYSSDAFGCEQGM 127
F H R DR++Y+ INW+NI +NF + L+ Y+ S+MHY DAF G
Sbjct: 177 FQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 236
Query: 126 N--TMIQTSGELIDYNHNTTPNDLNMIRNLYQC 34
+ T I I + + D+ I LY C
Sbjct: 237 DSITPIPNPNVPIGQRNGMSRWDITRINVLYNC 269
>sp|P55115|NAS15_CAEEL Zinc metalloproteinase nas-15 precursor (Nematode astacin 15)
Length = 571
Score = 83.2 bits (204), Expect = 7e-16
Identities = 62/200 (31%), Positives = 100/200 (50%), Gaps = 5/200 (2%)
Frame = -1
Query: 618 YKHHQGDIRIWPRGQVLFAFAQNFPYDKQNLVRQCLGELQSDLGGCIQFFESTEGPFIGV 439
Y + +++WP G++ + + + ++L+ + E S CI++ V
Sbjct: 113 YNAIKNRLQLWPEGRIPYTISSQYSSYSRSLIAASMQEYASHT--CIRWVPKEAADVNYV 170
Query: 438 HAQ-DKGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRRDRNNYIM 262
H D+GC++M+G G Q L+L + C+ KG I HE +HA+GF H R DR+++I
Sbjct: 171 HIYPDRGCYSMVGKMGGK-QSLSLGSG-CIQ-KGIILHELMHAVGFFHEQSRTDRDDHIT 227
Query: 261 INWDNIKSDQCFNFE-ICNGCI--LDGDYETRSVMHYSSDAFGCEQGMNTMI-QTSGELI 94
I W+NI++ FE +G I L Y+ S+MHY + AF G TMI + +G I
Sbjct: 228 IMWNNIQAGMQGQFEKYGHGTIQSLGTGYDYGSIMHYGTKAFS-RNGQPTMIPKKNGATI 286
Query: 93 DYNHNTTPNDLNMIRNLYQC 34
+ + D I LY C
Sbjct: 287 GQRNGFSKVDKFKINTLYGC 306
>sp|P31581|HCE21_ORYLA High choriolytic enzyme 2 precursor (Hatching enzyme zinc-protease
HCE 2 subunit) (Choriolysin H 2)
Length = 279
Score = 82.8 bits (203), Expect = 9e-16
Identities = 56/153 (36%), Positives = 77/153 (50%), Gaps = 3/153 (1%)
Frame = -1
Query: 483 CIQFFEST-EGPFIGVHAQDKGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHALG 307
CI+F T E FI V +++ GC++ +G G Q L+L CM S G I+HE HALG
Sbjct: 129 CIRFVRRTNEYDFISVVSKN-GCYSELGRKGGQ-QELSLNRGGCMYS-GIIQHELNHALG 185
Query: 306 FMHTHMRRDRNNYIMINWDNIKSDQCFNFEICNGCILDGDYETRSVMHYSSDAFGCEQGM 127
F H R DR++Y+ INW NI +NF + L+ Y+ S+MHY DAF G
Sbjct: 186 FQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFSIAYGR 245
Query: 126 N--TMIQTSGELIDYNHNTTPNDLNMIRNLYQC 34
+ T I I + + D+ LY C
Sbjct: 246 DSITPIPNPNVPIGQRNGMSRWDITRSNVLYNC 278
>sp|Q61847|MEP1B_MOUSE Meprin A beta-subunit precursor (Endopeptidase-2)
Length = 704
Score = 80.9 bits (198), Expect = 3e-15
Identities = 59/196 (30%), Positives = 93/196 (47%), Gaps = 6/196 (3%)
Frame = -1
Query: 603 GDIRIWPRGQVLFAFAQNFPYDKQNLVRQCLGELQSDLGGCIQFFE-STEGPFIGVHAQD 427
GD + WP + + + + + ++ + L CI F S E +I V +
Sbjct: 67 GDHKRWPH-TIPYVLEDSLEMNAKGVILNAFERYR--LKTCIDFKPWSGEANYISVF-KG 122
Query: 426 KGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRRDRNNYIMINWDN 247
GC++ +G Q L++ + T++HEF+HALGF H R DR++Y++I WD
Sbjct: 123 SGCWSSVGNIHAGKQELSIGTN--CDRIATVQHEFLHALGFWHEQSRADRDDYVIIVWDR 180
Query: 246 IKSDQCFNFEICNGCILDG---DYETRSVMHYSSDAF--GCEQGMNTMIQTSGELIDYNH 82
I+ + NF I N + D Y+ SVMHYS AF G E + T I ++I
Sbjct: 181 IQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQNGTESTIVTRISEFEDVIGQRM 240
Query: 81 NTTPNDLNMIRNLYQC 34
+ + DL + LY C
Sbjct: 241 DFSDYDLLKLNQLYNC 256
>sp|P28826|MEP1B_RAT Meprin A beta-subunit precursor (Endopeptidase-2)
Length = 704
Score = 79.7 bits (195), Expect = 7e-15
Identities = 56/156 (35%), Positives = 78/156 (50%), Gaps = 6/156 (3%)
Frame = -1
Query: 483 CIQFFE-STEGPFIGVHAQDKGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHALG 307
CI F S E +I V + GC++ +G Q L++ + T++HEF+HALG
Sbjct: 104 CIDFKPWSGEENYISVF-KGSGCWSSVGNIHAGKQELSIGTN--CDRIATVQHEFLHALG 160
Query: 306 FMHTHMRRDRNNYIMINWDNIKSDQCFNFEICNGCILDG---DYETRSVMHYSSDAF--G 142
F H R DR++YI I WD I S + NF I N + D Y+ SVMHYS AF G
Sbjct: 161 FWHEQSRADRDDYITIVWDRILSGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQNG 220
Query: 141 CEQGMNTMIQTSGELIDYNHNTTPNDLNMIRNLYQC 34
E + T I ++I + + DL + LY C
Sbjct: 221 TESTIITKISDFEDVIGQRMDFSDYDLLKLNQLYSC 256
>sp|P55112|NAS4_CAEEL Zinc metalloproteinase nas-4 precursor (Nematode astacin 4)
Length = 315
Score = 79.3 bits (194), Expect = 1e-14
Identities = 61/226 (26%), Positives = 105/226 (46%), Gaps = 12/226 (5%)
Frame = -1
Query: 675 NVLVGDMLLTKADIAVISGYKHHQGDI----RIWPRGQVLFAFAQNFPYDKQNLVRQCLG 508
N GD+LL V K + I R WP ++ + + + ++++ +
Sbjct: 71 NYSEGDILLESPKKFVEENNKLGRNAIKQIYRRWPNNEIPYTLSSQYGSYARSVIANAMN 130
Query: 507 ELQSDLGGCIQFFE---STEGPFIGVHAQDKGCFAMIGFTGEYGQFLNLQNPWCMNSKGT 337
E + C++F S ++ +H D+GC++++G TG G+ + C+ GT
Sbjct: 131 EYHTKT--CVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTG--GKQPVSLDSGCIQV-GT 184
Query: 336 IKHEFIHALGFMHTHMRRDRNNYIMINWDNIKSDQCFNFEICNGCI---LDGDYETRSVM 166
I HE +HA+GF H R+DR++YI + W N+ + FE N + LD Y+ S+M
Sbjct: 185 IVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIM 244
Query: 165 HYSSDAFGCEQGMNTMI--QTSGELIDYNHNTTPNDLNMIRNLYQC 34
HY AF G T++ ++ E + + D+ I LY C
Sbjct: 245 HYGPYAFS-GSGKKTLVPKKSGSERMGQRVKFSDIDVRKINKLYNC 289
>sp|Q6HA08|ASTL_HUMAN Astacin-like metalloendopeptidase precursor (Oocyte astacin)
(Ovastacin)
Length = 431
Score = 76.3 bits (186), Expect = 8e-14
Identities = 58/214 (27%), Positives = 93/214 (43%), Gaps = 8/214 (3%)
Frame = -1
Query: 651 LTKADIAVISGYKHHQGDIRIWPRG-----QVLFAFAQNFPYDKQNLVRQCLGELQSDLG 487
L + DI S ++ WP G +V F + + + ++ + L E +
Sbjct: 73 LIEGDIIRPSPFRLLSAASNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERST- 131
Query: 486 GCIQFFE-STEGPFIGVHAQDKGCFAMIGFTGEYGQFLNLQNPWCMNSKGTIKHEFIHAL 310
CI+F + FI + GCF+ +G +G Q ++L +G + HE +H L
Sbjct: 132 -CIRFVTYQDQRDFISIIPM-YGCFSSVGRSGGM-QVVSLAPTCLQKGRGIVLHELMHVL 188
Query: 309 GFMHTHMRRDRNNYIMINWDNIKSDQCFNFEICNGCILDGDYETRSVMHYSSDAFGCEQG 130
GF H H R DR+ YI +NW+ I NF + Y+ SVMHY AF +G
Sbjct: 189 GFWHEHTRADRDRYIRVNWNEILPGFEINFIKSRSSNMLTPYDYSSVMHYGRLAFS-RRG 247
Query: 129 MNTM--IQTSGELIDYNHNTTPNDLNMIRNLYQC 34
+ T+ + I N + +D+ + LY C
Sbjct: 248 LPTITPLWAPSVHIGQRWNLSASDITRVLKLYGC 281
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,547,292
Number of Sequences: 369166
Number of extensions: 1766034
Number of successful extensions: 4076
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3930
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4037
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6728100400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)