Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_M20
(524 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P41606|RPOC2_PINTH DNA-directed RNA polymerase beta'' ch... 34 0.25
sp|Q85X62|RPOC2_PINKO DNA-directed RNA polymerase beta'' ch... 33 0.42
sp|Q04351|Y3709_CLOAB Hypothetical protein CAC3709 32 0.93
sp|Q8TRB8|RPOA2_METAC DNA-directed RNA polymerase subunit A'' 31 1.6
sp|O33845|DPOL_THEAG DNA polymerase (Pol Tfu) [Contains: Ta... 30 2.7
sp|P84329|HFW1_DROPS Halfway protein (Singed wings protein) 30 3.5
sp|Q48514|TRA3_LEPBO Transposase for insertion sequence ele... 30 4.6
sp|P22967|ACET_MOUSE Angiotensin-converting enzyme, testis-... 30 4.6
sp|P09470|ACE_MOUSE Angiotensin-converting enzyme, somatic ... 30 4.6
sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1 29 6.0
>sp|P41606|RPOC2_PINTH DNA-directed RNA polymerase beta'' chain (PEP) (Plastid-encoded RNA
polymerase beta'' subunit) (RNA polymerase beta''
subunit)
Length = 1224
Score = 33.9 bits (76), Expect = 0.25
Identities = 21/77 (27%), Positives = 42/77 (54%), Gaps = 1/77 (1%)
Frame = +3
Query: 111 FNGQVLHEDYNSRYYNIRDGSVIQIVTGNLTNSINLIVEVQSIR-PNHNALYIKVDQNIT 287
FNG++ + R+G + NL+ +I+ +VQ++ P + L ++ DQ +
Sbjct: 373 FNGKIEFNENLVYPTRTRNGHPAYLCHNNLSITIDGQDQVQNLTIPPQSLLLVQNDQYVE 432
Query: 288 TEQLLSEIRTQISFIKD 338
+EQL++E+R + S K+
Sbjct: 433 SEQLIAEVRARTSSFKE 449
>sp|Q85X62|RPOC2_PINKO DNA-directed RNA polymerase beta'' chain (PEP) (Plastid-encoded RNA
polymerase beta'' subunit) (RNA polymerase beta''
subunit)
Length = 1209
Score = 33.1 bits (74), Expect = 0.42
Identities = 20/77 (25%), Positives = 42/77 (54%), Gaps = 1/77 (1%)
Frame = +3
Query: 111 FNGQVLHEDYNSRYYNIRDGSVIQIVTGNLTNSINLIVEVQSIR-PNHNALYIKVDQNIT 287
FNG++ + R+G + NL+ +I+ +VQ++ P + L ++ DQ +
Sbjct: 373 FNGKIEFNENLVYPTRTRNGHPAYLCHNNLSITIDGQNQVQNLTIPPQSLLLVQNDQYVE 432
Query: 288 TEQLLSEIRTQISFIKD 338
+EQ+++E+R + S K+
Sbjct: 433 SEQIIAEVRARTSSFKE 449
>sp|Q04351|Y3709_CLOAB Hypothetical protein CAC3709
Length = 1498
Score = 32.0 bits (71), Expect = 0.93
Identities = 24/84 (28%), Positives = 43/84 (51%), Gaps = 10/84 (11%)
Frame = +3
Query: 48 LIKSLKALWRDKYNLEIESLHFNG------QVLHEDYNSRYY----NIRDGSVIQIVTGN 197
LIK++ + DKY+ E E LHF + E YNSR Y + +D V+ + TGN
Sbjct: 306 LIKAVAEI-SDKYDEETEVLHFRNPSPEKLAEMIETYNSRIYERMASDKDFLVVTLGTGN 364
Query: 198 LTNSINLIVEVQSIRPNHNALYIK 269
+++++ ++ + +L +K
Sbjct: 365 QASNLSVDSDINDRDNDEKSLRVK 388
>sp|Q8TRB8|RPOA2_METAC DNA-directed RNA polymerase subunit A''
Length = 397
Score = 31.2 bits (69), Expect = 1.6
Identities = 28/115 (24%), Positives = 50/115 (43%), Gaps = 8/115 (6%)
Frame = +3
Query: 54 KSLKALWRDKYNLEIESLHFNGQVLHEDYNSRYYNIRDGSVIQIV--------TGNLTNS 209
+ L L + +N+ ++ + +V+ Y +GS ++ V T TN+
Sbjct: 208 RELLQLAKSIHNVTLKGIEGIKRVVVRKEGEEYTLYTEGSALRDVLQFEGVDKTRTSTNN 267
Query: 210 INLIVEVQSIRPNHNALYIKVDQNITTEQLLSEIRTQISFIKDSAYLTCRGQILQ 374
IN I EV I NA+ + + + L +IR I + D +TC G++ Q
Sbjct: 268 INEIYEVLGIEAARNAIIKEATDTLREQGLTVDIR-HIMLVAD--LMTCDGEVKQ 319
>sp|O33845|DPOL_THEAG DNA polymerase (Pol Tfu) [Contains: Tag pol-1 intein (Tsp-TY pol-1)
(Intein I); Tag pol-2 intein (Tsp-TY pol-2) (Intein II);
Tag pol-3 intein (Tsp-TY pol-3) (Intein III)]
Length = 1829
Score = 30.4 bits (67), Expect = 2.7
Identities = 22/80 (27%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = +3
Query: 51 IKSLKALWRDKYNLEIESLHFN-GQVLHEDYNSRYYNIRDGSVIQIVTGNLTNSINLIVE 227
IK +KAL R KY E + N G+ +H + IR+G + +I G +LI+
Sbjct: 918 IKKVKALIRHKYKGEAYEVELNSGRKIHITRGHSLFTIRNGKIKEI-WGEEVKVGDLIIV 976
Query: 228 VQSIRPNHNALYIKVDQNIT 287
+ ++ N I + + I+
Sbjct: 977 PKKVKLNEKEAVINIPELIS 996
>sp|P84329|HFW1_DROPS Halfway protein (Singed wings protein)
Length = 587
Score = 30.0 bits (66), Expect = 3.5
Identities = 26/87 (29%), Positives = 40/87 (45%)
Frame = +3
Query: 177 IQIVTGNLTNSINLIVEVQSIRPNHNALYIKVDQNITTEQLLSEIRTQISFIKDSAYLTC 356
+ + GN+T IN P H+AL NI+ + SEI +++ KD +L
Sbjct: 226 LAVTDGNITRLINAF-------PRHSALKCL---NISNNNI-SEIPSRM--FKDVPHLEF 272
Query: 357 RGQILQSASSLAHQNINNNDTIVVLMR 437
G + S + H N N N T+ + MR
Sbjct: 273 FGMSRNNLSLVPHHNQNKNITVDIRMR 299
>sp|Q48514|TRA3_LEPBO Transposase for insertion sequence element IS1533
Length = 346
Score = 29.6 bits (65), Expect = 4.6
Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
Frame = +3
Query: 9 EVNRFNETLDSSKLIKSLKAL-----WRDKYNLEIESLHFNGQVLHEDYNSRYYNIR 164
++ R + L L K +K L W + + +LHFN ++L E +N Y +R
Sbjct: 141 DLGRNRQRLMKFLLRKDIKLLPTTKYWTVSHYKWLNNLHFNNEILQETFNDYYSRVR 197
>sp|P22967|ACET_MOUSE Angiotensin-converting enzyme, testis-specific isoform precursor
(ACE-T) (Dipeptidyl carboxypeptidase I) (Kininase II)
[Contains: Angiotensin-converting enzyme,
testis-specific isoform, soluble form]
Length = 732
Score = 29.6 bits (65), Expect = 4.6
Identities = 24/96 (25%), Positives = 44/96 (45%), Gaps = 5/96 (5%)
Frame = +3
Query: 36 DSSKLIKSLKALWRDKYNLEIESLHFNGQVLHEDYNSRYYNIRDGSVIQIVTGNL----- 200
+S L + L+ L+++ L + + + LH Y S Y N+ DG + + GN+
Sbjct: 255 ESDNLEQDLEKLYQELQPLYLNLHAYVRRSLHRHYGSEYINL-DGPIPAHLLGNMWAQTW 313
Query: 201 TNSINLIVEVQSIRPNHNALYIKVDQNITTEQLLSE 308
+N +L+ S PN +A + Q T ++ E
Sbjct: 314 SNIYDLVAPFPS-APNIDATEAMIKQGWTPRRIFKE 348
>sp|P09470|ACE_MOUSE Angiotensin-converting enzyme, somatic isoform precursor (Dipeptidyl
carboxypeptidase I) (Kininase II) [Contains:
Angiotensin-converting enzyme, somatic isoform, soluble
form]
Length = 1312
Score = 29.6 bits (65), Expect = 4.6
Identities = 24/96 (25%), Positives = 44/96 (45%), Gaps = 5/96 (5%)
Frame = +3
Query: 36 DSSKLIKSLKALWRDKYNLEIESLHFNGQVLHEDYNSRYYNIRDGSVIQIVTGNL----- 200
+S L + L+ L+++ L + + + LH Y S Y N+ DG + + GN+
Sbjct: 835 ESDNLEQDLEKLYQELQPLYLNLHAYVRRSLHRHYGSEYINL-DGPIPAHLLGNMWAQTW 893
Query: 201 TNSINLIVEVQSIRPNHNALYIKVDQNITTEQLLSE 308
+N +L+ S PN +A + Q T ++ E
Sbjct: 894 SNIYDLVAPFPS-APNIDATEAMIKQGWTPRRIFKE 928
>sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1
Length = 785
Score = 29.3 bits (64), Expect = 6.0
Identities = 21/93 (22%), Positives = 40/93 (43%)
Frame = +3
Query: 171 SVIQIVTGNLTNSINLIVEVQSIRPNHNALYIKVDQNITTEQLLSEIRTQISFIKDSAYL 350
+ I++ N + + + VQS+ N +L K+ I ++ + F+KD+
Sbjct: 703 ATIRVSKPNENDGQFMEITVQSLSENVGSLKEKIAGEIQIPANKQKLSGKAGFLKDNM-- 760
Query: 351 TCRGQILQSASSLAHQNINNNDTIVVLMRTRGG 449
SLAH N+ + + + +R RGG
Sbjct: 761 -----------SLAHYNVGAGEILTLSLRERGG 782
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,213,201
Number of Sequences: 369166
Number of extensions: 1099944
Number of successful extensions: 2648
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2590
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2648
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)