Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_002_L13-2 (146 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q13200|PSD2_HUMAN 26S proteasome non-ATPase regulatory s... 64 1e-10 sp|Q8VDM4|PSD2_MOUSE 26S proteasome non-ATPase regulatory s... 64 1e-10 sp|Q7S8R8|RPN1_NEUCR 26S proteasome regulatory subunit rpn-1 55 4e-08 sp|P87048|RPN1_SCHPO 26S proteasome regulatory subunit rpn1... 48 8e-06 sp|Q6FPV6|RPN1_CANGA 26S proteasome regulatory subunit RPN1 33 0.15 sp|P38764|RPN1_YEAST 26S proteasome regulatory subunit RPN1... 30 1.3
>sp|Q13200|PSD2_HUMAN 26S proteasome non-ATPase regulatory subunit 2 (26S proteasome regulatory subunit RPN1) (26S proteasome regulatory subunit S2) (26S proteasome subunit p97) (Tumor necrosis factor type 1 receptor associated protein 2) (55.11 protein) Length = 908 Score = 63.9 bits (154), Expect = 1e-10 Identities = 30/48 (62%), Positives = 37/48 (77%) Frame = +2 Query: 2 GLLHLGKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRN 145 GL HLGKGTLT+ P+H DR LMS VAV GLLTVL+S LDV+ + ++ Sbjct: 767 GLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKS 814
>sp|Q8VDM4|PSD2_MOUSE 26S proteasome non-ATPase regulatory subunit 2 (26S proteasome regulatory subunit RPN1) (26S proteasome regulatory subunit S2) (26S proteasome subunit p97) Length = 908 Score = 63.9 bits (154), Expect = 1e-10 Identities = 30/48 (62%), Positives = 37/48 (77%) Frame = +2 Query: 2 GLLHLGKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRN 145 GL HLGKGTLT+ P+H DR LMS VAV GLLTVL+S LDV+ + ++ Sbjct: 767 GLTHLGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKS 814
>sp|Q7S8R8|RPN1_NEUCR 26S proteasome regulatory subunit rpn-1 Length = 883 Score = 55.5 bits (132), Expect = 4e-08 Identities = 24/48 (50%), Positives = 36/48 (75%) Frame = +2 Query: 2 GLLHLGKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLDVKRTFMNRN 145 GLLH+GKGTL++SP H DR ++S VA GLL VL++ +D K+ +++ Sbjct: 740 GLLHMGKGTLSVSPFHTDRQVLSNVATAGLLAVLVAMIDAKQFITSKS 787
>sp|P87048|RPN1_SCHPO 26S proteasome regulatory subunit rpn1 (Proteasome non-ATPase subunit mts4) (19S regulatory cap region of 26S protease subunit 2) Length = 891 Score = 47.8 bits (112), Expect = 8e-06 Identities = 19/39 (48%), Positives = 31/39 (79%) Frame = +2 Query: 2 GLLHLGKGTLTISPHHQDRFLMSPVAVGGLLTVLISCLD 118 GLL+LGKGT+T++P+H +R ++ A GL+TV+++ LD Sbjct: 748 GLLYLGKGTMTLNPYHTERQILGQTAFAGLMTVVLAMLD 786
>sp|Q6FPV6|RPN1_CANGA 26S proteasome regulatory subunit RPN1 Length = 983 Score = 33.5 bits (75), Expect = 0.15 Identities = 16/38 (42%), Positives = 26/38 (68%) Frame = +2 Query: 2 GLLHLGKGTLTISPHHQDRFLMSPVAVGGLLTVLISCL 115 GL+HLGKGT+T+ + D +++ V + LLTVL+ + Sbjct: 843 GLVHLGKGTMTMDIFN-DAHVLNKVTLASLLTVLVGLI 879
>sp|P38764|RPN1_YEAST 26S proteasome regulatory subunit RPN1 (Proteasome non-ATPase subunit 1) Length = 993 Score = 30.4 bits (67), Expect = 1.3 Identities = 14/35 (40%), Positives = 23/35 (65%) Frame = +2 Query: 2 GLLHLGKGTLTISPHHQDRFLMSPVAVGGLLTVLI 106 GLLHLGKGT+T+ + D +++ V + +LT + Sbjct: 853 GLLHLGKGTMTMDVFN-DAHVLNKVTLASILTTAV 886
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 17,605,372 Number of Sequences: 369166 Number of extensions: 236249 Number of successful extensions: 594 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 591 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 594 length of database: 68,354,980 effective HSP length: 22 effective length of database: 64,290,810 effective search space used: 1671561060 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)