Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_L08
(787 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp||Q687E1_2 [Segment 2 of 2] Nucleotide pyrophosphatase/ph... 63 8e-10
sp|Q38924|PPAF_ARATH Iron(III)-zinc(II) purple acid phospha... 52 1e-06
sp|P80366|PPAF_PHAVU Iron(III)-zinc(II) purple acid phospha... 46 1e-04
sp|Q12546|PPA_ASPFI Acid phosphatase precursor (pH 6-optimu... 42 0.001
sp|P19274|VTPX_TTV1 Viral protein TPX 34 0.52
sp|P41586|PACR_HUMAN Pituitary adenylate cyclase-activating... 34 0.52
sp|P19275|VTP3_TTV1V Viral protein TPX 34 0.52
sp|Q50644|YP77_MYCTU Hypothetical protein Rv2577/MT2654 34 0.52
sp|Q05205|PPB_LYSEN Alkaline phosphatase precursor (APASE) 33 0.68
sp|P0AC44|DHSD_ECOLI Succinate dehydrogenase hydrophobic me... 32 1.5
>sp||Q687E1_2 [Segment 2 of 2] Nucleotide pyrophosphatase/phosphodiesterase
Length = 350
Score = 63.2 bits (152), Expect = 8e-10
Identities = 55/182 (30%), Positives = 85/182 (46%), Gaps = 16/182 (8%)
Frame = +3
Query: 279 WSEIHTFKTFP-PGSNWPTRYVVVGDMG-----------NLNARSLAAIQRETEK-GFYD 419
W++ +TF+ P PG N R +V GDMG N SL R E YD
Sbjct: 7 WAKPYTFRAPPTPGQNSLQRIIVFGDMGKAERDGSNEFANYQPGSLNTTDRLIEDLDNYD 66
Query: 420 MVLHVGDFAYNMDSYNAQTGDQFMRQIQPVAAQLPYMTVPGNHEEAYNFSNYRRRFTMPG 599
+V H+GD Y + Y +Q DQF Q+ P++A+ PYM GNHE ++ N F +
Sbjct: 67 IVFHIGDMPY-ANGYLSQW-DQFTAQVAPISAKKPYMVASGNHER--DWPNTGGFFDVKD 122
Query: 600 GDGEGQYFSFNVGYAHIIGFSSEFYYFTNYGFKQI---ENQYNWVIK*LERKPINRKIEP 770
GE + + Y + + F+Y +YG + +++++W E P + IE
Sbjct: 123 SGGECGVPAETM-YYYPAENRANFWYKVDYGMFRFCVGDSEHDW----REGTPQYKFIEE 177
Query: 771 CV 776
C+
Sbjct: 178 CL 179
>sp|Q38924|PPAF_ARATH Iron(III)-zinc(II) purple acid phosphatase precursor (PAP)
Length = 469
Score = 52.4 bits (124), Expect = 1e-06
Identities = 67/270 (24%), Positives = 112/270 (41%), Gaps = 19/270 (7%)
Frame = +3
Query: 18 YNQPEQIHLSIGSNI-NXXXXXXXXXXXXNESIVIYGPDKSMNLKQ-NGTVD--KFVDGG 185
+N P+Q+H++ G++ N V Y + + KQ TV+ +F +
Sbjct: 57 HNSPQQVHVTQGNHEGNGVIISWVTPVKPGSKTVQYWCENEKSRKQAEATVNTYRFFNYT 116
Query: 186 SEKRQIFMHRVILPNLLPFTQYSYTVGSRFGWSEIHTFKTFPP--GSNWPTRYVVVGDMG 359
S ++H ++ +L T+Y Y +GS WS F PP G + P + ++GD+G
Sbjct: 117 SG----YIHHCLIDDLEFDTKYYYEIGSG-KWSRRFWF-FIPPKSGPDVPYTFGLIGDLG 170
Query: 360 NLNARSLAAIQRETEKGFYDMVLHVGDFAY--NMDSYNAQTGDQFMRQIQPVAAQLPYMT 533
+ E G VL VGD +Y +++ D + R ++ A P++
Sbjct: 171 QTYDSNSTLSHYEMNPGKGQAVLFVGDLSYADRYPNHDNNRWDTWGRFVERSVAYQPWIW 230
Query: 534 VPGNHE--------EAYNFSNYRRRFTMP---GGDGEGQYFSFNVGYAHIIGFSSEFYYF 680
GNHE E F + R+ P G ++S A+II S +
Sbjct: 231 TAGNHEIDFVPDIGEIEPFKPFMNRYHTPHKASGSISPLWYSIKRASAYIIVMSC----Y 286
Query: 681 TNYGFKQIENQYNWVIK*LERKPINRKIEP 770
++YG QY W+ K E + +NR P
Sbjct: 287 SSYGI--YTPQYKWLEK--ELQGVNRTETP 312
>sp|P80366|PPAF_PHAVU Iron(III)-zinc(II) purple acid phosphatase (PAP)
Length = 432
Score = 46.2 bits (108), Expect = 1e-04
Identities = 64/262 (24%), Positives = 105/262 (40%), Gaps = 20/262 (7%)
Frame = +3
Query: 18 YNQPEQIHLSIGSNI-NXXXXXXXXXXXXNESIVIYGPDKSMN---LKQNGTVDKFVDGG 185
YN P+Q+H++ G + S V Y +K+ K + +F +
Sbjct: 24 YNAPQQVHITQGDLVGRAMIISWVTMDEPGSSAVRYWSEKNGRKRIAKGKMSTYRFFNYS 83
Query: 186 SEKRQIFMHRVILPNLLPFTQYSYTVGSRFGWSEIHTFKTFP-PGSNWPTRYVVVGDMGN 362
S F+H + L T+Y Y VG R + +F T P G + P + ++GD+G
Sbjct: 84 SG----FIHHTTIRKLKYNTKYYYEVGLR-NTTRRFSFITPPQTGLDVPYTFGLIGDLGQ 138
Query: 363 L--NARSLAAIQRETEKGFYDMVLHVGDFAY--NMDSYNAQTGDQFMRQIQPVAAQLPYM 530
+ +L+ + +KG VL VGD +Y +++ D + R + A P++
Sbjct: 139 SFDSNTTLSHYELSPKKG--QTVLFVGDLSYADRYPNHDNVRWDTWGRFTERSVAYQPWI 196
Query: 531 TVPGNHE--------EAYNFSNYRRRFTMPGGDGEGQ---YFSFNVGYAHIIGFSSEFYY 677
GNHE E F + R+ +P + ++S AHII SS Y
Sbjct: 197 WTAGNHEIEFAPEINETEPFKPFSYRYHVPYEASQSTSPFWYSIKRASAHIIVLSSHIAY 256
Query: 678 FTNYGFKQIENQYNWVIK*LER 743
QY W+ K L +
Sbjct: 257 GRG------TPQYTWLKKELRK 272
>sp|Q12546|PPA_ASPFI Acid phosphatase precursor (pH 6-optimum acid phosphatase) (APase6)
Length = 614
Score = 42.4 bits (98), Expect = 0.001
Identities = 59/255 (23%), Positives = 83/255 (32%), Gaps = 100/255 (39%)
Frame = +3
Query: 204 FMHRVILPNLLPFTQYSYTVGSRFG--WSEIHTFKTF-PPGSNWPTRYVVVGDMGNLNAR 374
F H V + L P T Y Y + + G SE+ +FKT P G V+ DMG NA
Sbjct: 138 FFHEVSIDGLEPDTTYYYQIPAANGTTQSEVLSFKTSRPAGHPGSFSVAVLNDMGYTNAH 197
Query: 375 SL--AAIQRETEKGFYDMVLHVGDFAYNMDSYNA-------------------------- 470
++ TE + H GD +Y D Y+
Sbjct: 198 GTHKQLVKAATEGTAF--AWHGGDLSYADDWYSGILACADDWPVCYNGTSSTLPGGGPLP 255
Query: 471 ------------------QTGDQ----------FMRQIQPVAAQLPYMTVPGNHE----- 551
Q GD + + + V ++PYM +PGNHE
Sbjct: 256 EEYKKPLPAGEIPDQGGPQGGDMSVLYESNWDLWQQWLNNVTLKIPYMVLPGNHEASCAE 315
Query: 552 --------EAY-------------------------NFSNYRRRFTMPGGDGEGQ---YF 623
AY NF+ Y+ RF MPG + G ++
Sbjct: 316 FDGPHNILTAYLNDDIANGTAPTDNLTYYSCPPSQRNFTAYQHRFRMPGPETGGVGNFWY 375
Query: 624 SFNVGYAHIIGFSSE 668
SF+ G AH + E
Sbjct: 376 SFDYGLAHFVSIDGE 390
>sp|P19274|VTPX_TTV1 Viral protein TPX
Length = 360
Score = 33.9 bits (76), Expect = 0.52
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = -1
Query: 322 LEPGGKVLNV*ISDQPNLDPTV*LYWVNGNKFGSIT 215
L PG + S PN+DPT+ LY+ NG+ + ++T
Sbjct: 59 LSPGQSITITASSGTPNIDPTIALYYNNGSSYSNLT 94
>sp|P41586|PACR_HUMAN Pituitary adenylate cyclase-activating polypeptide type I receptor
precursor (PACAP type I receptor) (PACAP-R-1)
Length = 468
Score = 33.9 bits (76), Expect = 0.52
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 4/67 (5%)
Frame = +3
Query: 297 FKTFPPGSNWPTRYVVVGDMGNLNARSLA---AIQRE-TEKGFYDMVLHVGDFAYNMDSY 464
F+ F P W T + D G+ N+ L+ + R TE G+ + H D A D Y
Sbjct: 81 FRIFNPDQVWETETIGESDFGDSNSLDLSDMGVVSRNCTEDGWSEPFPHYFD-ACGFDEY 139
Query: 465 NAQTGDQ 485
++TGDQ
Sbjct: 140 ESETGDQ 146
>sp|P19275|VTP3_TTV1V Viral protein TPX
Length = 474
Score = 33.9 bits (76), Expect = 0.52
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = -1
Query: 322 LEPGGKVLNV*ISDQPNLDPTV*LYWVNGNKFGSIT 215
L PG + S PN+DPT+ LY+ NG+ + ++T
Sbjct: 59 LSPGQSITITASSGTPNIDPTIALYYNNGSSYSNLT 94
>sp|Q50644|YP77_MYCTU Hypothetical protein Rv2577/MT2654
Length = 529
Score = 33.9 bits (76), Expect = 0.52
Identities = 40/182 (21%), Positives = 62/182 (34%), Gaps = 25/182 (13%)
Frame = +3
Query: 198 QIFMHRVILPNLLPFTQYSYTVGSRFGWSEIHTFKTFPPGSNWPTRYVVVGDMG------ 359
++ ++ L NL P T Y Y E+ T +T P G P R+ GD
Sbjct: 123 EVRVNHAHLTNLTPDTDYVYAAVHDGTTPELGTARTAPSGRK-PLRFTSFGDQSTPALGR 181
Query: 360 ---------NLNARSLAAIQRETEKGFYDMVLHVGDFAY-NMDSYNAQTGDQFMRQIQPV 509
N+ + I E+ L GD Y N+ +T +
Sbjct: 182 LADGRYVSDNIGSPFAGDITIAIERIAPLFNLINGDLCYANLAQDRIRTWSDWFDNNTRS 241
Query: 510 AAQLPYMTVPGNHEE-----AYNFSNYRRRFTMPGGDG----EGQYFSFNVGYAHIIGFS 662
A P+M GNHE + Y+ F +P G ++SF G +I
Sbjct: 242 ARYRPWMPAAGNHENEVGNGPIGYDAYQTYFAVPDSGSSPQLRGLWYSFTAGSVRVISLH 301
Query: 663 SE 668
++
Sbjct: 302 ND 303
>sp|Q05205|PPB_LYSEN Alkaline phosphatase precursor (APASE)
Length = 539
Score = 33.5 bits (75), Expect = 0.68
Identities = 29/121 (23%), Positives = 54/121 (44%), Gaps = 12/121 (9%)
Frame = +3
Query: 339 VVVGDMGNLNARSLAAIQRETEKGFYDMVLHVGD---FAYNMDSYNAQTGDQFMRQIQPV 509
VVV G++ S A Q G D+++ + F ++YN+ T ++ + P
Sbjct: 151 VVVAGAGDICDTSGNACQ-----GTSDLIVSINPTAVFTAGDNAYNSGTLSEYNSRYAPT 205
Query: 510 AAQLPYMTVP--GNHEEA-------YNFSNYRRRFTMPGGDGEGQYFSFNVGYAHIIGFS 662
+ +T P GNH+ + +++ N T P GD Y+S++VG H + +
Sbjct: 206 WGRFKALTSPSPGNHDYSTTGAKGYFDYFNGSGNQTGPAGDRSKGYYSWDVGDWHFVSLN 265
Query: 663 S 665
+
Sbjct: 266 T 266
>sp|P0AC44|DHSD_ECOLI Succinate dehydrogenase hydrophobic membrane anchor protein
sp|P0AC46|DHSD_SHIFL Succinate dehydrogenase hydrophobic membrane anchor protein
sp|P0AC45|DHSD_ECOL6 Succinate dehydrogenase hydrophobic membrane anchor protein
Length = 115
Score = 32.3 bits (72), Expect = 1.5
Identities = 13/34 (38%), Positives = 22/34 (64%), Gaps = 2/34 (5%)
Frame = +2
Query: 203 FYASSYTTEF--VAIYPIQLHCWIKIWLVRNSYI 298
F+AS++T F +A++ I +H WI +W V Y+
Sbjct: 51 FFASAFTKVFTLLALFSILIHAWIGMWQVLTDYV 84
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,351,112
Number of Sequences: 369166
Number of extensions: 2078970
Number of successful extensions: 4391
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4389
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7405750800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)