Planarian EST Database


Dr_sW_002_J15

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_002_J15
         (625 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P00889|CISY_PIG  Citrate synthase, mitochondrial precursor     212   5e-55
sp|O75390|CISY_HUMAN  Citrate synthase, mitochondrial precursor   209   4e-54
sp|Q9CZU6|CISY_MOUSE  Citrate synthase, mitochondrial precursor   209   5e-54
sp|P23007|CISY_CHICK  Citrate synthase, mitochondrial             209   6e-54
sp|Q9M1D3|CISY5_ARATH  Citrate synthase 5, mitochondrial pre...   201   1e-51
sp|P34575|CISY_CAEEL  Probable citrate synthase, mitochondri...   200   3e-51
sp|O00098|CISY_EMENI  Citrate synthase, mitochondrial precursor   198   8e-51
sp|P00890|CISY1_YEAST  Citrate synthase, mitochondrial precu...   198   1e-50
sp|P34085|CISY_NEUCR  Citrate synthase, mitochondrial precursor   197   1e-50
sp|P51044|CISY_ASPNG  Citrate synthase, mitochondrial precursor   197   2e-50
>sp|P00889|CISY_PIG Citrate synthase, mitochondrial precursor
          Length = 464

 Score =  212 bits (540), Expect = 5e-55
 Identities = 100/138 (72%), Positives = 120/138 (86%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           +D+ + ++I  TL +G+VVPGYGHAVLRKTDPRY CQREFALKHLP  P+FK+V+QLY+I
Sbjct: 324 SDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPHDPMFKLVAQLYKI 383

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
           VP+VL ++ K  NP+PNVDAHSGVLLQYYGMTE NYYTVLFG+SRALG+L+ LIW RALG
Sbjct: 384 VPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALG 443

Query: 365 LPIERPKSFSTDKLIELV 418
            P+ERPKS STD LI+LV
Sbjct: 444 FPLERPKSMSTDGLIKLV 461
>sp|O75390|CISY_HUMAN Citrate synthase, mitochondrial precursor
          Length = 466

 Score =  209 bits (533), Expect = 4e-54
 Identities = 100/143 (69%), Positives = 123/143 (86%), Gaps = 1/143 (0%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           +D+ + ++I  TL +G+VVPGYGHAVLRKTDPRY CQREFALKHLP+ P+FK+V+QLY+I
Sbjct: 324 SDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPNDPMFKLVAQLYKI 383

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
           VP+VL ++ K  NP+PNVDAHSGVLLQYYGMTE NYYTVLFG+SRALG+L+ LIW RALG
Sbjct: 384 VPNVLLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALG 443

Query: 365 LPIERPKSFSTDKLIELV-RKSG 430
            P+ERPKS ST+ L++ V  KSG
Sbjct: 444 FPLERPKSMSTEGLMKFVDSKSG 466
>sp|Q9CZU6|CISY_MOUSE Citrate synthase, mitochondrial precursor
          Length = 464

 Score =  209 bits (532), Expect = 5e-54
 Identities = 97/138 (70%), Positives = 119/138 (86%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           +D+ + ++I  TL +G+VVPGYGHAVLRKTDPRY CQREFALKHLP  P+FK+V+QLY+I
Sbjct: 324 SDEKLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYSCQREFALKHLPKDPMFKLVAQLYKI 383

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
           VP++L ++ K  NP+PNVDAHSGVLLQYYGMTE NYYTVLFG+SRALG+L+ LIW RALG
Sbjct: 384 VPNILLEQGKAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALG 443

Query: 365 LPIERPKSFSTDKLIELV 418
            P+ERPKS STD L++ V
Sbjct: 444 FPLERPKSMSTDGLMKFV 461
>sp|P23007|CISY_CHICK Citrate synthase, mitochondrial
          Length = 433

 Score =  209 bits (531), Expect = 6e-54
 Identities = 99/136 (72%), Positives = 116/136 (85%)
 Frame = +2

Query: 8   DKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQIV 187
           D  + ++I  TL +G+VVPGYGHAVLRKTDPRY CQREFALKHLP  P+FK+V+QLY+IV
Sbjct: 298 DASLRDYIWNTLNSGRVVPGYGHAVLRKTDPRYTCQREFALKHLPGDPMFKLVAQLYKIV 357

Query: 188 PDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALGL 367
           P+VL ++   ANP+PNVDAHSGVLLQYYGMTE NYYTVLFG+SRALG+L+ LIW RALG 
Sbjct: 358 PNVLLEQGAAANPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGF 417

Query: 368 PIERPKSFSTDKLIEL 415
           P+ERPKS STD LI L
Sbjct: 418 PLERPKSMSTDGLIAL 433
>sp|Q9M1D3|CISY5_ARATH Citrate synthase 5, mitochondrial precursor
          Length = 433

 Score =  201 bits (512), Expect = 1e-51
 Identities = 92/137 (67%), Positives = 114/137 (83%)
 Frame = +2

Query: 11  KDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQIVP 190
           + + +++ KTL +G+VVPGYGH VLRKTDPRY+CQREFALKHLPD PLF++VS+LY++VP
Sbjct: 294 EQLKDYVWKTLNSGKVVPGYGHGVLRKTDPRYICQREFALKHLPDDPLFQLVSKLYEVVP 353

Query: 191 DVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALGLP 370
            +L +  K+ NP+PNVDAHSGVLL YYG+TE  YYTVLFG+SR+LGI S LIWDRALGLP
Sbjct: 354 PILTELGKVKNPWPNVDAHSGVLLNYYGLTEARYYTVLFGVSRSLGICSQLIWDRALGLP 413

Query: 371 IERPKSFSTDKLIELVR 421
           +ERPKS + D L    R
Sbjct: 414 LERPKSVNMDWLDNFTR 430
>sp|P34575|CISY_CAEEL Probable citrate synthase, mitochondrial precursor
          Length = 468

 Score =  200 bits (508), Expect = 3e-51
 Identities = 95/137 (69%), Positives = 116/137 (84%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           T++ + E++ K LK+GQVVPGYGHAVLRKTDPRY CQREFALKHLP+  LFK+VS LY+I
Sbjct: 326 TEEQLKEWVWKHLKSGQVVPGYGHAVLRKTDPRYECQREFALKHLPNDDLFKLVSTLYKI 385

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
            P +L ++ K  NP+PNVD+HSGVLLQY+GMTE ++YTVLFG+SRALG LS LIW R +G
Sbjct: 386 TPGILLEQGKAKNPWPNVDSHSGVLLQYFGMTEMSFYTVLFGVSRALGCLSQLIWARGMG 445

Query: 365 LPIERPKSFSTDKLIEL 415
           LP+ERPKS STD LI+L
Sbjct: 446 LPLERPKSHSTDGLIKL 462
>sp|O00098|CISY_EMENI Citrate synthase, mitochondrial precursor
          Length = 474

 Score =  198 bits (504), Expect = 8e-51
 Identities = 95/138 (68%), Positives = 113/138 (81%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           +D+ I +++  TL AG+VVPGYGHAVLRKTDPRY  QREFAL+ LPD P+FK+VSQ+Y+I
Sbjct: 333 SDQSIKDYLWSTLNAGRVVPGYGHAVLRKTDPRYTSQREFALRKLPDDPMFKLVSQVYKI 392

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
            P VL +  K  NP+PNVDAHSGVLLQYYG+TE NYYTVLFG+SRALG+L  LI DRA G
Sbjct: 393 APGVLTEHGKTKNPYPNVDAHSGVLLQYYGLTEANYYTVLFGVSRALGVLPQLIIDRAFG 452

Query: 365 LPIERPKSFSTDKLIELV 418
            PIERPKSFST+   +LV
Sbjct: 453 APIERPKSFSTEAYAKLV 470
>sp|P00890|CISY1_YEAST Citrate synthase, mitochondrial precursor
          Length = 479

 Score =  198 bits (503), Expect = 1e-50
 Identities = 93/136 (68%), Positives = 113/136 (83%)
 Frame = +2

Query: 17  IVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQIVPDV 196
           I +++  TL AG+VVPGYGHAVLRKTDPRY  QREFALKH PDY LFK+VS +Y++ P V
Sbjct: 339 IEKYLWDTLNAGRVVPGYGHAVLRKTDPRYTAQREFALKHFPDYELFKLVSTIYEVAPGV 398

Query: 197 LGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALGLPIE 376
           L K  K  NP+PNVD+HSGVLLQYYG+TE ++YTVLFG++RA+G+L  LI DRA+G PIE
Sbjct: 399 LTKHGKTKNPWPNVDSHSGVLLQYYGLTEASFYTVLFGVARAIGVLPQLIIDRAVGAPIE 458

Query: 377 RPKSFSTDKLIELVRK 424
           RPKSFST+K  ELV+K
Sbjct: 459 RPKSFSTEKYKELVKK 474
>sp|P34085|CISY_NEUCR Citrate synthase, mitochondrial precursor
          Length = 469

 Score =  197 bits (502), Expect = 1e-50
 Identities = 92/140 (65%), Positives = 117/140 (83%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           +D+ I +++  TL AG+VVPGYGHAVLRKTDPRY  QR+FA +HLP+ P+F++VSQ+Y+I
Sbjct: 329 SDEAITKYLWDTLNAGRVVPGYGHAVLRKTDPRYSAQRKFAQEHLPEDPMFQLVSQVYKI 388

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
            P VL +  K  NP+PNVDAHSGVLLQ+YG+TE NYYTVLFG+SRA+G+L  LI DRA+G
Sbjct: 389 APKVLTEHGKTKNPYPNVDAHSGVLLQHYGLTEANYYTVLFGVSRAIGVLPQLIIDRAVG 448

Query: 365 LPIERPKSFSTDKLIELVRK 424
            PIERPKS+STDK IE+ +K
Sbjct: 449 APIERPKSYSTDKWIEICKK 468
>sp|P51044|CISY_ASPNG Citrate synthase, mitochondrial precursor
          Length = 475

 Score =  197 bits (501), Expect = 2e-50
 Identities = 94/132 (71%), Positives = 111/132 (84%)
 Frame = +2

Query: 5   TDKDIVEFIEKTLKAGQVVPGYGHAVLRKTDPRYMCQREFALKHLPDYPLFKVVSQLYQI 184
           +D+ I  ++  TL AGQVVPGYGHAVLRKTDPRY+ QREFAL+ LPD P+FK+VSQ+Y+I
Sbjct: 333 SDEAIKNYLWSTLNAGQVVPGYGHAVLRKTDPRYVSQREFALRKLPDDPMFKLVSQVYKI 392

Query: 185 VPDVLGKKSKIANPFPNVDAHSGVLLQYYGMTETNYYTVLFGISRALGILSSLIWDRALG 364
            P VL +  K  NP+PNVDAHSGVLLQYYG+TE NYYTVLFG+SRALG+L  LI DRALG
Sbjct: 393 APGVLTEHGKTKNPYPNVDAHSGVLLQYYGLTEANYYTVLFGVSRALGVLPQLIIDRALG 452

Query: 365 LPIERPKSFSTD 400
            PIERPKS+ST+
Sbjct: 453 APIERPKSYSTE 464
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,525,926
Number of Sequences: 369166
Number of extensions: 1258294
Number of successful extensions: 3427
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3328
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3383
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)