Planarian EST Database


Dr_sW_002_J04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_002_J04
         (383 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P37167|ACTP_ACACA  Actophorin                                   54   1e-07
sp|P18359|DEST_CHICK  Destrin (Actin-depolymerizing factor) ...    53   2e-07
sp|Q9LQ81|ADFX_ARATH  Actin-depolymerizing factor like At1g0...    52   5e-07
sp|P60982|DEST_PIG  Destrin (Actin-depolymerizing factor) (A...    50   1e-06
sp|Q9Y281|COF2_HUMAN  Cofilin-2 (Cofilin, muscle isoform)          50   1e-06
sp|P23528|COF1_HUMAN  Cofilin-1 (Cofilin, non-muscle isoform...    50   1e-06
sp|P45591|COF2_MOUSE  Cofilin-2 (Cofilin, muscle isoform)          50   1e-06
sp|P45592|COF1_RAT  Cofilin-1 (Cofilin, non-muscle isoform)        50   1e-06
sp|Q9R0P5|DEST_MOUSE  Destrin (Actin-depolymerizing factor) ...    50   1e-06
sp|P18760|COF1_MOUSE  Cofilin-1 (Cofilin, non-muscle isoform)      50   2e-06
>sp|P37167|ACTP_ACACA Actophorin
          Length = 138

 Score = 53.5 bits (127), Expect = 1e-07
 Identities = 28/98 (28%), Positives = 48/98 (48%), Gaps = 8/98 (8%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGCYALYDYE--------GTLAFISWIPETMKINSRMIMA 161
           G     YE FK  + E   +   YA++DYE          + FI W P++  I S+M+  
Sbjct: 43  GGPNATYEDFKSQLPERDCR---YAIFDYEFQVDGGQRNKITFILWAPDSAPIKSKMMYT 99

Query: 162 ASKSEIATRMVGVKAKIEANSEDEIDESVIKEKTASKV 275
           ++K  I  ++VG++ +++A    EI E  + E+    V
Sbjct: 100 STKDSIKKKLVGIQVEVQATDAAEISEDAVSERAKKDV 137
>sp|P18359|DEST_CHICK Destrin (Actin-depolymerizing factor) (ADF)
          Length = 165

 Score = 53.1 bits (126), Expect = 2e-07
 Identities = 30/94 (31%), Positives = 48/94 (51%), Gaps = 9/94 (9%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYDY--------EGTLAFISWIPETMKINSRMIM 158
           GD+G       +H +E   ++ C YALYD         +  L F  W PE   + S+MI 
Sbjct: 58  GDVGVTVTDPFKHFVEMLPEKDCRYALYDASFETKESKKEELMFFLWAPEQAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 260
           A+SK  I  +  G+K + +AN  ++++ + I EK
Sbjct: 118 ASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
          Length = 140

 Score = 51.6 bits (122), Expect = 5e-07
 Identities = 26/90 (28%), Positives = 46/90 (51%), Gaps = 9/90 (10%)
 Frame = +3

Query: 12  LGTPYEKFKQHIIETYAQEGCYALYDYEGT---------LAFISWIPETMKINSRMIMAA 164
           LG P E ++         E  YA+YDY+ T         + FI+W P+T ++ S+M+ A+
Sbjct: 46  LGNPEETYEDFTRSIPEDECRYAVYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYAS 105

Query: 165 SKSEIATRMVGVKAKIEANSEDEIDESVIK 254
           SK      + G++ +++A    E+   +IK
Sbjct: 106 SKDRFKRELDGIQVELQATDPSEMSLDIIK 135
>sp|P60982|DEST_PIG Destrin (Actin-depolymerizing factor) (ADF)
 sp|P60981|DEST_HUMAN Destrin (Actin-depolymerizing factor) (ADF)
          Length = 165

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 29/94 (30%), Positives = 47/94 (50%), Gaps = 9/94 (9%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYDY--------EGTLAFISWIPETMKINSRMIM 158
           GD+G       +H +    ++ C YALYD         +  L F  W PE   + S+MI 
Sbjct: 58  GDVGVTITDPFKHFVGMLPEKDCRYALYDASFETKESRKEELMFFLWAPELAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 260
           A+SK  I  +  G+K + +AN  ++++ + I EK
Sbjct: 118 ASSKDAIKKKFQGIKHECQANGPEDLNRACIAEK 151
>sp|Q9Y281|COF2_HUMAN Cofilin-2 (Cofilin, muscle isoform)
          Length = 166

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 35/100 (35%), Positives = 48/100 (48%), Gaps = 10/100 (10%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIM 158
           GD+G   E      ++      C YALYD  YE        L FI W PE+  + S+MI 
Sbjct: 58  GDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEI-DESVIKEKTASKV 275
           A+SK  I  +  G+K + + N  D+I D S + EK    V
Sbjct: 118 ASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNV 157
>sp|P23528|COF1_HUMAN Cofilin-1 (Cofilin, non-muscle isoform) (18 kDa phosphoprotein)
           (p18)
          Length = 166

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 32/95 (33%), Positives = 50/95 (52%), Gaps = 10/95 (10%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIM 158
           GD+G   +      ++    + C YALYD  YE        L FI W PE+  + S+MI 
Sbjct: 58  GDVGQTVDDPYATFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEI-DESVIKEK 260
           A+SK  I  ++ G+K +++AN  +E+ D   + EK
Sbjct: 118 ASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
>sp|P45591|COF2_MOUSE Cofilin-2 (Cofilin, muscle isoform)
          Length = 166

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 35/100 (35%), Positives = 48/100 (48%), Gaps = 10/100 (10%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIM 158
           GD+G   E      ++      C YALYD  YE        L FI W PE+  + S+MI 
Sbjct: 58  GDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEI-DESVIKEKTASKV 275
           A+SK  I  +  G+K + + N  D+I D S + EK    V
Sbjct: 118 ASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGSV 157
>sp|P45592|COF1_RAT Cofilin-1 (Cofilin, non-muscle isoform)
          Length = 166

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 32/95 (33%), Positives = 50/95 (52%), Gaps = 10/95 (10%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIM 158
           GD+G   +      ++    + C YALYD  YE        L FI W PE+  + S+MI 
Sbjct: 58  GDVGQTVDDPYTTFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEI-DESVIKEK 260
           A+SK  I  ++ G+K +++AN  +E+ D   + EK
Sbjct: 118 ASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
>sp|Q9R0P5|DEST_MOUSE Destrin (Actin-depolymerizing factor) (ADF) (Sid 23)
          Length = 165

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 29/94 (30%), Positives = 47/94 (50%), Gaps = 9/94 (9%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYDY--------EGTLAFISWIPETMKINSRMIM 158
           GD+G       +H +    ++ C YALYD         +  L F  W PE   + S+MI 
Sbjct: 58  GDVGATITDPFKHFVGMLPEKDCRYALYDASFETKESRKEELMFFLWAPEQAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEIDESVIKEK 260
           A+SK  I  +  G+K + +AN  ++++ + I EK
Sbjct: 118 ASSKDAIKKKFPGIKHEYQANGPEDLNRTCIAEK 151
>sp|P18760|COF1_MOUSE Cofilin-1 (Cofilin, non-muscle isoform)
          Length = 166

 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 32/95 (33%), Positives = 49/95 (51%), Gaps = 10/95 (10%)
 Frame = +3

Query: 6   GDLGTPYEKFKQHIIETYAQEGC-YALYD--YEGT------LAFISWIPETMKINSRMIM 158
           GD+G   +      ++    + C YALYD  YE        L FI W PE   + S+MI 
Sbjct: 58  GDVGQTVDDPYTTFVKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPENAPLKSKMIY 117

Query: 159 AASKSEIATRMVGVKAKIEANSEDEI-DESVIKEK 260
           A+SK  I  ++ G+K +++AN  +E+ D   + EK
Sbjct: 118 ASSKDAIKKKLTGIKHELQANCYEEVKDRCTLAEK 152
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,971,640
Number of Sequences: 369166
Number of extensions: 573142
Number of successful extensions: 1415
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1392
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1412
length of database: 68,354,980
effective HSP length: 94
effective length of database: 50,989,890
effective search space used: 1682666370
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)