Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_F24
(839 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATP... 248 1e-65
sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATP... 239 9e-63
sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATP... 233 6e-61
sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATP... 231 2e-60
sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATP... 216 8e-56
sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATP... 207 2e-53
sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATP... 164 4e-40
sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATP... 163 5e-40
sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATP... 152 1e-36
sp|O23948|VATE_GOSHI Vacuolar ATP synthase subunit E (V-ATP... 152 1e-36
>sp|P54611|VATE_DROME Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 248 bits (634), Expect = 1e-65
Identities = 126/226 (55%), Positives = 168/226 (74%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 199
MAL++ DVQKQIK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKK 60
Query: 200 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 379
EK++E +KKIQ SNM+NQARLK+L +++HV VL +A+KRL +T + +Y+ L +LI
Sbjct: 61 EKQVELQKKIQSSNMLNQARLKVLKVREDHVSSVLDDARKRLGEVTKNQSEYETVLTKLI 120
Query: 380 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 559
QGLFQ++E KVI+RC D+ LV +L Y+A+ VEL I++ + L D GG
Sbjct: 121 VQGLFQIMEPKVILRCREVDVPLVRNVLPAAVEQYKAQINQNVELFIDEKDFLSADTCGG 180
Query: 560 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
VEL NGR+K+ NTLESRLDLISQQL+PEIR LFG+N NR F D
Sbjct: 181 VELLALNGRIKVPNTLESRLDLISQQLVPEIRNALFGRNVNRKFTD 226
>sp|P31402|VATE_MANSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 28 kDa subunit)
Length = 226
Score = 239 bits (609), Expect = 9e-63
Identities = 124/226 (54%), Positives = 165/226 (73%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 199
MAL++ DVQKQIK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQQQRLKIMEYYEKK 60
Query: 200 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 379
EK++E +KKIQ SNM+NQARLK+L +++HV++VL EA+KRL + D + Y + L LI
Sbjct: 61 EKQVELQKKIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDIKLYSDLLVTLI 120
Query: 380 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 559
Q LFQL+E V +R + D LVE +L Y+AK + V L I+ +N LP D GG
Sbjct: 121 VQALFQLVEPTVTLRVRQADKALVESLLGRAQQDYKAKIKKDVVLKIDNENFLPPDTCGG 180
Query: 560 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
+EL GR+KI NTLESRL+LI+QQL+PEIR LFG+N NR F D
Sbjct: 181 IELIAAKGRIKISNTLESRLELIAQQLLPEIRNALFGRNPNRKFTD 226
>sp|P36543|VATE_HUMAN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 233 bits (593), Expect = 6e-61
Identities = 115/226 (50%), Positives = 170/226 (75%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 199
MAL++ DVQKQIK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKK 60
Query: 200 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 379
EK++EQ+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+
Sbjct: 61 EKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLV 120
Query: 380 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 559
QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I++++ LP+D+AGG
Sbjct: 121 LQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGG 180
Query: 560 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
VE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 181 VEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|P11019|VATE_BOVIN Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 226
Score = 231 bits (589), Expect = 2e-60
Identities = 114/226 (50%), Positives = 169/226 (74%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 199
MAL++ DVQKQIK+M++FIEQEA EK +EI++KA+EEF IEK RLVQ QR K+ E++ K+
Sbjct: 1 MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKK 60
Query: 200 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 379
EK++EQ+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L L+
Sbjct: 61 EKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLV 120
Query: 380 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 559
QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I+++ LP+++AGG
Sbjct: 121 LQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPVYKVATKRDVDVQIDQEAYLPEEIAGG 180
Query: 560 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
VE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 181 VEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 226
>sp|Q9U1G5|VATE_HETSC Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 216 bits (549), Expect = 8e-56
Identities = 106/226 (46%), Positives = 163/226 (72%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 199
M ++++DVQKQ+++M++FIEQEA EK +EI++KA+EEF IEK +LVQ QR K+ E++ K+
Sbjct: 1 MGISDNDVQKQLRHMMAFIEQEANEKAEEIDAKAEEEFNIEKGKLVQLQRQKIMEYYEKK 60
Query: 200 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 379
EK++E ++KIQ SNM NQ+RLK L + +H+K+VL EA+ L ++ D ++Y LK L+
Sbjct: 61 EKQVELQRKIQRSNMQNQSRLKCLKARDDHLKNVLEEARANLSKISADRERYPAILKGLL 120
Query: 380 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 559
QGLFQLLE+KV++RC + D +V IL EC + + E+ I+ ++ L + AGG
Sbjct: 121 LQGLFQLLESKVVLRCRKKDEEMVARILPECLEEVQRTWGNRSEVKIDNEHFLSPESAGG 180
Query: 560 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
VEL +G++++ +TLE+RLDLI+ ++ P++R LFG N NR F D
Sbjct: 181 VELLAKDGKIRVSSTLEARLDLIADKITPQVRTALFGPNQNRRFFD 226
>sp|P50518|VATE_MOUSE Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 31 kDa subunit) (P31)
Length = 228
Score = 207 bits (528), Expect = 2e-53
Identities = 108/228 (47%), Positives = 164/228 (71%), Gaps = 2/228 (0%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDE-INSKADEEFEIEKSRLVQDQRSKMNEFFSK 196
M L + DVQKQIK+M++FIEQEA EK +E I++KA+EEF IEK RL++ QR K+ E++ K
Sbjct: 1 MGLRHADVQKQIKHMMAFIEQEANEKAEEEIDAKAEEEFNIEKGRLLETQRLKIMEYYEK 60
Query: 197 EEKKL-EQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKR 373
+EK++ +Q+KKIQ SN+MNQARLK+L + + + D+L EA++RL + D +Y+ L
Sbjct: 61 KEKQIRQQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLMKVVKDTTRYQVLLDG 120
Query: 374 LITQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLA 553
L+ QGL+QLLE ++I+RC + D LV+ + + Y+ T+ V++ I+++ ++A
Sbjct: 121 LVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKRDVDVQIDQEPTCLRNIA 180
Query: 554 GGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
GGVE+ + ++K+ NTLESRLDLI+QQ++PE+R LFG N NR F D
Sbjct: 181 GGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD 228
>sp|O94072|VATE_CANAL Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 226
Score = 164 bits (414), Expect = 4e-40
Identities = 84/226 (37%), Positives = 143/226 (63%)
Frame = +2
Query: 20 MALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKE 199
MAL+++ V+ ++ M +FIE+EAKEK EI KADEE+EIEK+ +V+ + + ++ + ++
Sbjct: 1 MALSDEQVKSELSKMQAFIEKEAKEKAKEIKLKADEEYEIEKASIVRSETAAIDSTYEQK 60
Query: 200 EKKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLI 379
KK ++I S + N+ RL+IL + E + ++ EA+ L +T D ++YK L LI
Sbjct: 61 LKKASLAQQITKSTIGNKTRLRILSTKDEVLHEIFDEAEAELKKITKDKKQYKPVLVGLI 120
Query: 380 TQGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGG 559
+G+ L+E KV I+ D+ + + + E A +E K + KVE++I+ N L D+AGG
Sbjct: 121 EEGVLALMEPKVSIKVREQDVDVAKEAITEAAKNFEEKAKFKVEISIDDKNFLAKDIAGG 180
Query: 560 VELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
+ + +G++++ NTLE RL ++S++ +P IR LFG +T R F D
Sbjct: 181 IVVVNGSGKIEVDNTLEERLKILSEEALPAIRLELFGPSTTRKFFD 226
>sp|Q01278|VATE_NEUCR Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit) (V-ATPase 26 kDa subunit)
Length = 230
Score = 163 bits (413), Expect = 5e-40
Identities = 83/225 (36%), Positives = 142/225 (63%)
Frame = +2
Query: 23 ALTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEE 202
AL++D V ++++ M +FI+QEA+EK EI KADEEF IEKS+LV+ + ++ ++K+
Sbjct: 6 ALSDDQVGQELRKMTAFIKQEAEEKAREIQIKADEEFAIEKSKLVRQETDAIDSAYAKKF 65
Query: 203 KKLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLIT 382
K+ + ++I S M N+ RL++LG +QE + ++ A +L T+D +YK+ L+ LI
Sbjct: 66 KQAQMSQQITRSTMANKTRLRVLGARQELLDEIFEAASAQLGQATHDLGRYKDILRDLIL 125
Query: 383 QGLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDDLAGGV 562
+G + + E +++IR + D V ++ Y+ KT+ V+ TI+ +N +P+ AGG+
Sbjct: 126 EGFYAMNEPELVIRARQADYDAVREAAGWASAQYKHKTDKDVKATIDAENPVPEGSAGGI 185
Query: 563 ELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNTNRIFND 697
+ NG++ I NT E+RL L+ +P +R+ LFG+N NR F D
Sbjct: 186 IIVGGNGKIDIDNTFEARLTLLKDSALPAMRKALFGENPNRKFFD 230
>sp|Q39258|VATE_ARATH Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 230
Score = 152 bits (384), Expect = 1e-36
Identities = 85/229 (37%), Positives = 137/229 (59%), Gaps = 11/229 (4%)
Frame = +2
Query: 26 LTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEK 205
+ + DV +QI+ M+ FI QEA+EK +EI+ A+EEF IEK +LV+ ++ K+ + + K+EK
Sbjct: 1 MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKKEK 60
Query: 206 KLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQ 385
+ + RKKI +S +N +R+K+L Q + V + +A K L N++ D YK+ LK LI Q
Sbjct: 61 QADVRKKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKDLIVQ 120
Query: 386 GLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKV-ELTINKDNCLPD------ 544
L +L E V++RC D+ LVE +LD+ Y K ++ E+ ++ LP
Sbjct: 121 CLLRLKEPSVLLRCREEDLGLVEAVLDDAKEEYAGKAKVHAPEVAVDTKIFLPPPPKSND 180
Query: 545 ----DLAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGKNT 679
+GGV L +G++ NTL++RLD+ + +P IR+ LFG+ T
Sbjct: 181 PHGLHCSGGVVLASRDGKIVCENTLDARLDVAFRMKLPVIRKSLFGQVT 229
>sp|O23948|VATE_GOSHI Vacuolar ATP synthase subunit E (V-ATPase E subunit) (Vacuolar
proton pump E subunit)
Length = 237
Score = 152 bits (383), Expect = 1e-36
Identities = 86/234 (36%), Positives = 136/234 (58%), Gaps = 18/234 (7%)
Frame = +2
Query: 26 LTNDDVQKQIKNMISFIEQEAKEKVDEINSKADEEFEIEKSRLVQDQRSKMNEFFSKEEK 205
+++ DV KQI+ M+ FI QEA+EK +EI+ A+EEF IEK +LV+ ++ K+ + + K+EK
Sbjct: 1 MSDADVSKQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60
Query: 206 KLEQRKKIQHSNMMNQARLKILGCQQEHVKDVLTEAQKRLHNLTNDPQKYKETLKRLITQ 385
++E RKKI++S +N +R+K+L Q + V + A K N+++D YK LK LI Q
Sbjct: 61 QVEIRKKIEYSMQLNASRIKVLQAQDDVVNAMKESASKDFLNVSHDHHVYKRLLKDLIVQ 120
Query: 386 GLFQLLEAKVIIRCCRGDIRLVEVILDECASAYEAKTEMKVELTINKDNCLPDD------ 547
L +L E V++RC + D+ LVE +LD Y +K + I D LP
Sbjct: 121 SLVRLKEPGVLLRCRKEDLHLVESVLDSAKEEYASKVNVHPPEIIVDDVHLPPGPSHHHG 180
Query: 548 ------------LAGGVELCVPNGRVKIVNTLESRLDLISQQLIPEIREILFGK 673
+GGV + +G++ NTL++RLD+ + +PEIR+ LFG+
Sbjct: 181 FFHHHAEAHGPFCSGGVVIASRDGKIVFENTLDARLDVAFNKKLPEIRKWLFGQ 234
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,914,660
Number of Sequences: 369166
Number of extensions: 1653084
Number of successful extensions: 6613
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6502
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8197207050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)