Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_E06
(766 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P54677|PI4K_DICDI Phosphatidylinositol 4-kinase (PI4-kin... 35 0.29
sp|P39937|PAC2_YEAST Protein PAC2 33 0.65
sp|Q9Y5T5|UBP16_HUMAN Ubiquitin carboxyl-terminal hydrolase... 33 0.85
sp|Q12527|ATG11_YEAST Autophagy-related protein 11 (Cytopla... 33 0.85
sp|P07248|ADR1_YEAST Regulatory protein ADR1 33 0.85
sp|Q9J5D0|MP44_FOWPV Probable metalloendopeptidase G1-type 32 1.4
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 32 2.5
sp|Q09312|FBF2_CAEEL Fem-3 mRNA binding factor 2 32 2.5
sp|P41001|TOP2_PLAFK DNA topoisomerase 2 (DNA topoisomerase... 31 3.2
sp|P31332|L_SYNV Large structural protein (L protein) (Tran... 31 4.2
>sp|P54677|PI4K_DICDI Phosphatidylinositol 4-kinase (PI4-kinase) (PtdIns-4-kinase)
(PI4K-alpha)
Length = 1093
Score = 34.7 bits (78), Expect = 0.29
Identities = 25/98 (25%), Positives = 50/98 (51%)
Frame = +2
Query: 11 IQSTPKLKSLNCSNSELSKSVNDENSTFDSKINTLLKDSKLDNEYNTISITDQNDNLSNF 190
I++ PK K +N +N+ + N+ ++ D+ N +L + DN SI D+N+ N
Sbjct: 175 IENDPK-KDINSNNNNNNNINNNNSNNDDNNNNEILPNENSDN-----SINDENNQYGN- 227
Query: 191 IQINTSKPEINYNGKYISLRGSISAQKKSNYSLRNSSS 304
S N +G+ +++ I++Q KS+ ++ +S
Sbjct: 228 -----SNNNNNISGENNNIKIDINSQNKSDSNIETLNS 260
>sp|P39937|PAC2_YEAST Protein PAC2
Length = 518
Score = 33.5 bits (75), Expect = 0.65
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Frame = +2
Query: 29 LKSLNCSNSELSKSVNDENSTFDSKINTLLKDSKLDNEYNTISITDQ-NDNLSNFIQINT 205
LK LN SN+++S++ + + +S T DN++ S+ D N + I+
Sbjct: 278 LKGLNVSNNQISRAPSIAIYSVESLDIT-------DNKFKERSLIDDLNKTFPSLKNIHL 330
Query: 206 SKPEINYNGKYISL 247
S E NYNG YI++
Sbjct: 331 SGNEFNYNGNYINV 344
>sp|Q9Y5T5|UBP16_HUMAN Ubiquitin carboxyl-terminal hydrolase 16 (Ubiquitin thiolesterase
16) (Ubiquitin-specific processing protease 16)
(Deubiquitinating enzyme 16) (Ubiquitin processing
protease UBP-M)
Length = 823
Score = 33.1 bits (74), Expect = 0.85
Identities = 14/41 (34%), Positives = 25/41 (60%)
Frame = +2
Query: 122 DSKLDNEYNTISITDQNDNLSNFIQINTSKPEINYNGKYIS 244
+S DN+ +T + +N N+ N +++ TS P N NG Y++
Sbjct: 523 ESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLT 563
>sp|Q12527|ATG11_YEAST Autophagy-related protein 11 (Cytoplasm to vacuole targeting protein
9)
Length = 1178
Score = 33.1 bits (74), Expect = 0.85
Identities = 31/127 (24%), Positives = 61/127 (48%), Gaps = 22/127 (17%)
Frame = +2
Query: 11 IQSTPKLKSLNCSNSELSKSVNDE----------NSTFDSKINTLL-----KDSKLDNEY 145
++ T ++L N EL++ N+E N++F ++N ++ K KL +Y
Sbjct: 743 VEKTAYRETLTNLNQELARLTNEEQSHRTEIFTLNASFKKQLNDIISQDNEKIEKLTGDY 802
Query: 146 NTISITDQN-------DNLSNFIQINTSKPEINYNGKYISLRGSISAQKKSNYSLRNSSS 304
+ +S + + N + ++N K +I GK I ++++K +Y+ NSSS
Sbjct: 803 DDVSKSRERLQMDLDESNKKHEQEVNLLKADIERLGKQI-----VTSEK--SYAETNSSS 855
Query: 305 MDKMFEF 325
M+K +F
Sbjct: 856 MEKGEKF 862
>sp|P07248|ADR1_YEAST Regulatory protein ADR1
Length = 1323
Score = 33.1 bits (74), Expect = 0.85
Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 6/57 (10%)
Frame = +2
Query: 161 TDQNDNLSNFIQINTSK---PEINYNGKYI---SLRGSISAQKKSNYSLRNSSSMDK 313
T N N N NT + PE N KY+ + R S SAQ S+Y+L + SS+++
Sbjct: 193 TPDNGNFLNRTTANTRRKASPEANVKRKYLKKLTRRASFSAQSASSYALPDQSSLEQ 249
>sp|Q9J5D0|MP44_FOWPV Probable metalloendopeptidase G1-type
Length = 626
Score = 32.3 bits (72), Expect = 1.4
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = -3
Query: 686 LVYKMSNIGIICTSAIYSFHFSIYSSLGTNIFRIFFVMLDTICKNCANTANLNRVIQNNT 507
L+Y +++I + ++ Y F F+I+ N FFV L I C +N N I+ +
Sbjct: 558 LIYYLTSIKLGISNTFYIFAFTIFPEKVYN----FFVGLKEITNRCLIVSNKNTKIEEDD 613
Query: 506 CDSLRTAIV 480
SL IV
Sbjct: 614 YSSLNKQIV 622
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 31.6 bits (70), Expect = 2.5
Identities = 27/95 (28%), Positives = 39/95 (41%), Gaps = 1/95 (1%)
Frame = +2
Query: 14 QSTPKLKSLNCSNSELSKSVNDENSTFDSKINTLLKDSKLDNEYNTISITDQNDNL-SNF 190
+S KLKS S+ + + + S D + +S L + S TD+ + L S
Sbjct: 226 KSASKLKSSRSSSESIKSKLKNSKSQEDISNSRSTSESALSPTKSGPSKTDEKNFLHSTS 285
Query: 191 IQINTSKPEINYNGKYISLRGSISAQKKSNYSLRN 295
T Y +ISLRGS S+ S S N
Sbjct: 286 THQKTKSASSLYRRSFISLRGSSSSNASSAKSPSN 320
>sp|Q09312|FBF2_CAEEL Fem-3 mRNA binding factor 2
Length = 632
Score = 31.6 bits (70), Expect = 2.5
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = -2
Query: 147 LYSLSNFESFSNVLILESKVEFSSFTDLD-NSELLQFKDFNLGVDCIN 7
L SL +F+SF NV + S+ SSF D +S L+ DF+ V C +
Sbjct: 83 LMSLRSFQSFPNVFMPVSRSRTSSFIQSDTDSSRLESDDFSQNVRCFS 130
>sp|P41001|TOP2_PLAFK DNA topoisomerase 2 (DNA topoisomerase II)
Length = 1398
Score = 31.2 bits (69), Expect = 3.2
Identities = 25/74 (33%), Positives = 34/74 (45%), Gaps = 3/74 (4%)
Frame = +2
Query: 17 STPKLKSLNCSNSELSKSVNDENSTFDS--KINTLLKDSKLDNEYNTISITDQNDNLSNF 190
+TP + LN N+E S + D ST + K + DS DN S NDN+++
Sbjct: 1311 NTPLINRLNDENNEFSSNNVDNKSTNKNSRKKKPKIADSTNDNNSELNSSIQINDNVNDD 1370
Query: 191 IQINTS-KPEINYN 229
I I S IN N
Sbjct: 1371 INITISPNKTINVN 1384
>sp|P31332|L_SYNV Large structural protein (L protein) (Transcriptase) (Replicase)
[Includes: RNA-directed RNA polymerase ; mRNA
(guanine-N(7)-)-methyltransferase ; mRNA
guanylyltransferase ]
Length = 2116
Score = 30.8 bits (68), Expect = 4.2
Identities = 25/79 (31%), Positives = 40/79 (50%), Gaps = 5/79 (6%)
Frame = +2
Query: 26 KLKSLNCSNSELSKSVNDENSTFDS-KINTLLK--DSKLDNEYNTISITDQN--DNLSNF 190
K K+++ + SEL +SVN +NS FDS K +LK + + D YN + D D
Sbjct: 502 KDKAISMTRSELIESVNTKNSVFDSTKRRGILKWLNEQSDKIYNFLMRIDDKGLDEDDCI 561
Query: 191 IQINTSKPEINYNGKYISL 247
I + + E+ ++ SL
Sbjct: 562 IGLYPKEREMKTKARFFSL 580
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,921,211
Number of Sequences: 369166
Number of extensions: 1649388
Number of successful extensions: 4599
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4391
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4587
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7066925600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)