Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_E03-2
(442 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O08547|SC221_MOUSE Vesicle trafficking protein SEC22b (S... 145 5e-35
sp|Q94AU2|SC22_ARATH 25.3 kDa vesicle transport protein (At... 106 2e-23
sp|Q9Y7L0|SEC22_SCHPO Protein transport protein sec22 105 4e-23
sp|Q6CJA0|SEC22_KLULA Protein transport protein SEC22 94 1e-19
sp|Q74ZD2|SEC22_ASHGO Protein transport protein SEC22 93 2e-19
sp|Q6C880|SEC22_YARLI Protein transport protein SEC22 92 7e-19
sp|Q6FWT0|SEC22_CANGA Protein transport protein SEC22 82 7e-16
sp|P22214|SEC22_YEAST Protein transport protein SEC22 (Prot... 73 2e-13
sp|O49377|V711_ARATH Vesicle-associated membrane protein 71... 42 5e-04
sp|Q9SIQ9|V712_ARATH Vesicle-associated membrane protein 71... 42 5e-04
>sp|O08547|SC221_MOUSE Vesicle trafficking protein SEC22b (SEC22 vesicle trafficking
protein-like 1)
sp|Q5RAI9|SC221_PONPY Vesicle trafficking protein SEC22b (SEC22 vesicle trafficking
protein-like 1)
sp|O75396|SC221_HUMAN Vesicle trafficking protein SEC22b (SEC22 vesicle trafficking
protein-like 1)
Length = 215
Score = 145 bits (365), Expect = 5e-35
Identities = 69/132 (52%), Positives = 93/132 (70%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCC 181
DGL LAASMQEDE+ + L YQ QAKQL +K N SP+ ++E+ + FHY I++ VC
Sbjct: 12 DGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVCY 71
Query: 182 IILTEKEFSKKMAFAFLTDIMGEFYNEHGSKIGIVSRPYAFIEFDRYIQKAKKIYTDTRT 361
++L E F KK+AFA+L D+ EF +HG K+ VSRPY+FIEFD +IQK KK+Y D+R
Sbjct: 72 LVLCEAAFPKKLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSRA 131
Query: 362 SRNISQLNYELE 397
RN+ +N EL+
Sbjct: 132 RRNLGSINTELQ 143
>sp|Q94AU2|SC22_ARATH 25.3 kDa vesicle transport protein (AtSEC22)
Length = 218
Score = 106 bits (265), Expect = 2e-23
Identities = 55/133 (41%), Positives = 81/133 (60%), Gaps = 2/133 (1%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVG--SIESDSYMFHYFIDREV 175
DGL LA + +D + + + Y+ Q K L K + S+E+ Y+FHY I+ V
Sbjct: 12 DGLPLAEGL-DDGRDLPDSDMYKQQVKALFKNLSRGQNDASRMSVETGPYVFHYIIEGRV 70
Query: 176 CCIILTEKEFSKKMAFAFLTDIMGEFYNEHGSKIGIVSRPYAFIEFDRYIQKAKKIYTDT 355
C + + ++ + KK+AF +L D+ EF +G I +RPYAFI+FD +IQK KK+Y DT
Sbjct: 71 CYLTMCDRSYPKKLAFQYLEDLKNEFERVNGPNIETAARPYAFIKFDTFIQKTKKLYQDT 130
Query: 356 RTSRNISQLNYEL 394
RT RNI++LN EL
Sbjct: 131 RTQRNIAKLNDEL 143
>sp|Q9Y7L0|SEC22_SCHPO Protein transport protein sec22
Length = 209
Score = 105 bits (262), Expect = 4e-23
Identities = 52/131 (39%), Positives = 86/131 (65%), Gaps = 1/131 (0%)
Frame = +2
Query: 8 LVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCCII 187
L LAAS+ +DE ++L ++ QAK +LK+ + S SIES Y FHY ID +C +
Sbjct: 7 LPLAASV-DDESTERNLESHKKQAKLILKRLSPTSEKRASIESGDYTFHYLIDNGICYLC 65
Query: 188 LTEKEFSKKMAFAFLTDIMGEFYNEHGSK-IGIVSRPYAFIEFDRYIQKAKKIYTDTRTS 364
+ E+ + +K+AF++L ++ GEF+N G + + RPYAF++FD ++QK+K++Y R +
Sbjct: 66 ICEQSYPRKLAFSYLEELAGEFWNSFGEEALQPGLRPYAFVQFDTFMQKSKRVYNTPRAN 125
Query: 365 RNISQLNYELE 397
N+ +LN EL+
Sbjct: 126 DNLDKLNTELK 136
>sp|Q6CJA0|SEC22_KLULA Protein transport protein SEC22
Length = 214
Score = 94.0 bits (232), Expect = 1e-19
Identities = 50/146 (34%), Positives = 83/146 (56%), Gaps = 1/146 (0%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCC 181
DGL L AS+ +D SL + + K L+ +F S + ++ES + HY V
Sbjct: 11 DGLPLCASVDDDTD--PSLQEQKKKVKILISRFTPTSANEATVESGDFEIHYIRLNTVVY 68
Query: 182 IILTEKEFSKKMAFAFLTDIMGEFYNEHGSKIGIVS-RPYAFIEFDRYIQKAKKIYTDTR 358
I++ E+ + + +AF++L DI EF + +G ++ + RPY F+ FD ++QK KK+Y D R
Sbjct: 69 IVVAERNYPRNLAFSYLADIQQEFEHSYGGQLSKSNVRPYEFVSFDNFLQKTKKVYNDKR 128
Query: 359 TSRNISQLNYELEGCAKESW*KTLDD 436
N+ QLN +L+G K+ K ++D
Sbjct: 129 VQGNMDQLNQDLKG-VKQIMTKNIED 153
>sp|Q74ZD2|SEC22_ASHGO Protein transport protein SEC22
Length = 214
Score = 93.2 bits (230), Expect = 2e-19
Identities = 52/146 (35%), Positives = 85/146 (58%), Gaps = 1/146 (0%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCC 181
DGL L +S+ +D SL + + K L+ +F S + ++ES +Y HY + V
Sbjct: 11 DGLPLCSSVDDDTD--PSLADQKKKVKVLISRFTPQSANEATLESGAYEMHYVRQQSVVY 68
Query: 182 IILTEKEFSKKMAFAFLTDIMGEFYNEHGSKIGIVS-RPYAFIEFDRYIQKAKKIYTDTR 358
I++ E+ + + +AFA+L ++ EF + +G++ S RPYAF+ FD ++QK KKIY D R
Sbjct: 69 IVIVERGYPRNLAFAYLANVRHEFEHSYGNEYVKPSVRPYAFVSFDNFLQKTKKIYNDKR 128
Query: 359 TSRNISQLNYELEGCAKESW*KTLDD 436
N+ QLN +L G K+ K ++D
Sbjct: 129 VQGNLDQLNSDLLG-VKQIMTKNIED 153
>sp|Q6C880|SEC22_YARLI Protein transport protein SEC22
Length = 213
Score = 91.7 bits (226), Expect = 7e-19
Identities = 48/135 (35%), Positives = 82/135 (60%), Gaps = 3/135 (2%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCC 181
DGL L+AS+ + ++ +L+ + Q K + +K + S SIES +Y+ HY + +
Sbjct: 12 DGLPLSASVDDGQE---NLSEQKKQCKLVTQKLSANSADRASIESGNYVIHYLMKDGIVY 68
Query: 182 IILTEKEFSKKMAFAFLTDIMGEFYNEHGS---KIGIVSRPYAFIEFDRYIQKAKKIYTD 352
++E + +K+AF++L ++ EF HG K G+ RPY F+EFD ++QK K++Y D
Sbjct: 69 YCISESSYPRKLAFSYLDELDREFQKSHGQEALKEGV--RPYKFVEFDTFMQKTKRVYQD 126
Query: 353 TRTSRNISQLNYELE 397
TR + N+ +LN EL+
Sbjct: 127 TRATHNLDKLNTELQ 141
>sp|Q6FWT0|SEC22_CANGA Protein transport protein SEC22
Length = 215
Score = 81.6 bits (200), Expect = 7e-16
Identities = 45/146 (30%), Positives = 81/146 (55%), Gaps = 1/146 (0%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCC 181
DGL L +S+ +D + SLN + + K ++ + S + ++ES HY + V
Sbjct: 11 DGLPLCSSVDDDISDL-SLNEQKKRIKMVVSRMTPQSANEATLESKDSEIHYIRQQGVIY 69
Query: 182 IILTEKEFSKKMAFAFLTDIMGEFYNEHGSKIGIVS-RPYAFIEFDRYIQKAKKIYTDTR 358
++ E + + +AF++L D+ EF + + +++ + RPYAF FD ++Q+ KK Y+D +
Sbjct: 70 FVICEAGYPRNLAFSYLNDVAVEFQHSYSNELSKPTIRPYAFASFDTFLQRTKKAYSDKK 129
Query: 359 TSRNISQLNYELEGCAKESW*KTLDD 436
N+ QLN EL G K+ K ++D
Sbjct: 130 VQDNLDQLNQELVG-VKQIMSKNIED 154
>sp|P22214|SEC22_YEAST Protein transport protein SEC22 (Protein SLY2)
Length = 214
Score = 73.2 bits (178), Expect = 2e-13
Identities = 44/146 (30%), Positives = 75/146 (51%), Gaps = 1/146 (0%)
Frame = +2
Query: 2 DGLVLAASMQEDEKIIKSLNHYQCQAKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCC 181
DGL L S+ + SL + + K ++ + S + ++ES S+ HY V
Sbjct: 11 DGLPLCTSVDNEND--PSLFEQKQKVKIVVSRLTPQSATEATLESGSFEIHYLKKSMVYY 68
Query: 182 IILTEKEFSKKMAFAFLTDIMGEFYNEHGSKIGIVS-RPYAFIEFDRYIQKAKKIYTDTR 358
++ E + + +AF++L DI EF + ++ + RPY F+ FD ++Q KK Y+D +
Sbjct: 69 FVICESGYPRNLAFSYLNDIAQEFEHSFANEYPKPTVRPYQFVNFDNFLQMTKKSYSDKK 128
Query: 359 TSRNISQLNYELEGCAKESW*KTLDD 436
N+ QLN EL G K+ K ++D
Sbjct: 129 VQDNLDQLNQELVG-VKQIMSKNIED 153
>sp|O49377|V711_ARATH Vesicle-associated membrane protein 711 (AtVAMP711) (v-SNARE
synaptobrevin 7C) (AtVAMP7C)
Length = 219
Score = 42.4 bits (98), Expect = 5e-04
Identities = 32/124 (25%), Positives = 55/124 (44%), Gaps = 3/124 (2%)
Frame = +2
Query: 77 AKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCCIILTEKEFSKKMAFAFLTDIMGEFY 256
AKQ+L+K + S S D Y+FH + + + E+ +++ FAFL DI F
Sbjct: 29 AKQILEKVPGDNDSNVSYSQDRYVFHVKRTDGLTVLCMAEETAGRRIPFAFLEDIHQRFV 88
Query: 257 NEHGSKIGIVSRPYAFIEFDRYIQKAKKIYTDTRTSRNISQLNYEL---EGCAKESW*KT 427
+G + EF R + + Y++ + I+++ E+ G E+ K
Sbjct: 89 RTYGRAVHTALAYAMNEEFSRVLSQQIDYYSNDPNADRINRIKGEMNQVRGVMIENIDKV 148
Query: 428 LDDG 439
LD G
Sbjct: 149 LDRG 152
>sp|Q9SIQ9|V712_ARATH Vesicle-associated membrane protein 712 (AtVAMP712)
Length = 219
Score = 42.4 bits (98), Expect = 5e-04
Identities = 26/106 (24%), Positives = 49/106 (46%)
Frame = +2
Query: 77 AKQLLKKFNNLSPSVGSIESDSYMFHYFIDREVCCIILTEKEFSKKMAFAFLTDIMGEFY 256
AKQ+L+K S S D Y+FH + + + +++ +++ F+FL DI F
Sbjct: 29 AKQILEKIPGNGDSHVSYSQDRYVFHVKRTDGLTVLCMADEDAGRRIPFSFLEDIHQRFV 88
Query: 257 NEHGSKIGIVSRPYAFIEFDRYIQKAKKIYTDTRTSRNISQLNYEL 394
+G I EF R + + + Y++ + IS++ E+
Sbjct: 89 RTYGRAIHSAQAYAMNDEFSRVLNQQIEYYSNDPNADTISRIKGEM 134
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,208,851
Number of Sequences: 369166
Number of extensions: 801698
Number of successful extensions: 1780
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1774
length of database: 68,354,980
effective HSP length: 100
effective length of database: 49,881,480
effective search space used: 2294548080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)