Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_C21
(691 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CDC0|SPB13_MOUSE Serpin B13 32 2.1
sp|P25386|USO1_YEAST Intracellular protein transport protei... 32 2.1
sp|Q89AP2|RS2_BUCBP 30S ribosomal protein S2 31 2.7
sp|Q10664|MEK2_CAEEL Dual specificity mitogen-activated pro... 30 4.6
sp|Q89A96|MDLB_BUCBP Multidrug resistance-like ATP-binding ... 30 4.6
sp|Q9UIV8|SPB13_HUMAN Serpin B13 (Hurpin) (HaCaT UV-repress... 30 6.0
sp|P29508|SCCA1_HUMAN Squamous cell carcinoma antigen 1 (SC... 30 6.0
sp|Q6URW6|MYH14_MOUSE Myosin-14 (Myosin heavy chain, nonmus... 30 6.0
sp|P04540|NU5M_TRYBB NADH-ubiquinone oxidoreductase chain 5... 30 6.0
sp|Q94165|HM34_CAEEL Homeobox protein ceh-34 30 7.8
>sp|Q8CDC0|SPB13_MOUSE Serpin B13
Length = 389
Score = 31.6 bits (70), Expect = 2.1
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +2
Query: 77 EMKDLMKHNDEQQAKLNKSKDQETNLRVKELLPQTNRMLLEIVKSQALVLVN 232
E D + DE + K+N + +TN++VK+L P+ + + S LVL+N
Sbjct: 132 EPVDFVNAADESRKKINSWVESQTNVKVKDLFPEGS-----LNSSTKLVLIN 178
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
Length = 1790
Score = 31.6 bits (70), Expect = 2.1
Identities = 23/55 (41%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Frame = +2
Query: 56 KRKQLVREMKDLMKHNDEQQAKLNKSKDQETNLRVKELLP--QTNR-MLLEIVKS 211
K +L E+K MK E+Q+ L KS+ NL++KEL +TN LLE +KS
Sbjct: 1199 KNDELEGEVK-AMKSTSEEQSNLKKSEIDALNLQIKELKKKNETNEASLLESIKS 1252
>sp|Q89AP2|RS2_BUCBP 30S ribosomal protein S2
Length = 228
Score = 31.2 bits (69), Expect = 2.7
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = +2
Query: 26 WTNKMDNNW*KRKQLVREMKDLMKHNDEQQAKLNKSKDQETNLRVKELLPQTNRM 190
W M NW +Q ++ +KDL + Q NK +E LR++EL N +
Sbjct: 95 WLGGMLTNWKTVRQSIKRLKDL--ESQSQDGTFNKLTKKEVLLRMRELSKLENSL 147
>sp|Q10664|MEK2_CAEEL Dual specificity mitogen-activated protein kinase kinase mek-2 (MAP
kinase kinase mek-2)
Length = 387
Score = 30.4 bits (67), Expect = 4.6
Identities = 23/81 (28%), Positives = 43/81 (53%), Gaps = 8/81 (9%)
Frame = -3
Query: 227 LILTPDSLLFLAAYGLSVAVILSL*DLFLGPLTYLA---LPVVHRYVSS-----GLSFLE 72
+++ + + L +G+S +I S+ + F+G +Y+A L H +SS GLS +E
Sbjct: 201 MLVNSNGEIKLCDFGVSGMLIDSMANSFVGTRSYMAPERLTGSHYTISSDIWSFGLSLVE 260
Query: 71 LTVFVFTSCYPSYSSMSTLFN 9
L + + PS + +T+FN
Sbjct: 261 LLIGRYPVPAPSQAEYATMFN 281
>sp|Q89A96|MDLB_BUCBP Multidrug resistance-like ATP-binding protein mdlB
Length = 578
Score = 30.4 bits (67), Expect = 4.6
Identities = 9/23 (39%), Positives = 18/23 (78%)
Frame = +1
Query: 595 FLSHYFLTSLVNFTFLLVSYFLI 663
F+ HYF+ + VN++ +++Y+LI
Sbjct: 49 FIKHYFINNTVNYSLKIITYYLI 71
>sp|Q9UIV8|SPB13_HUMAN Serpin B13 (Hurpin) (HaCaT UV-repressible serpin) (Protease
inhibitor 13) (Headpin)
Length = 391
Score = 30.0 bits (66), Expect = 6.0
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = +2
Query: 77 EMKDLMKHNDEQQAKLNKSKDQETNLRVKELLPQTNRMLLEIVKSQALVLVN 232
E D + DE + K+N + +TN ++K+L P + I S LVLVN
Sbjct: 133 EPVDFVNAADESRKKINSWVESKTNEKIKDLFPDGS-----ISSSTKLVLVN 179
>sp|P29508|SCCA1_HUMAN Squamous cell carcinoma antigen 1 (SCCA-1) (Protein T4-A)
Length = 390
Score = 30.0 bits (66), Expect = 6.0
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +2
Query: 77 EMKDLMKHNDEQQAKLNKSKDQETNLRVKELLPQTNRMLLEIVKSQALVLVN 232
E D +E + K+N + +TN ++K L+P+ N I + LVLVN
Sbjct: 132 ESVDFANAPEESRKKINSWVESQTNEKIKNLIPEGN-----IGSNTTLVLVN 178
>sp|Q6URW6|MYH14_MOUSE Myosin-14 (Myosin heavy chain, nonmuscle IIc) (Nonmuscle myosin heavy
chain IIc) (NMHC II-C)
Length = 2000
Score = 30.0 bits (66), Expect = 6.0
Identities = 20/81 (24%), Positives = 40/81 (49%), Gaps = 9/81 (11%)
Frame = +2
Query: 56 KRKQLVREMKDLMKHNDEQ--QAKLNKSKDQETNLRVKELLPQTN-------RMLLEIVK 208
+R+QL ++++D DE+ Q L + ++ L ++EL QT+ + ++ K
Sbjct: 1611 RRRQLAKQLRDAEVERDEERKQRALAMAARKKLELELEELKAQTSAAGQGKEEAVKQLKK 1670
Query: 209 SQALVLVNWRTINKAYSSDDK 271
Q + WR + + SS D+
Sbjct: 1671 MQVQMKELWREVEETRSSRDE 1691
>sp|P04540|NU5M_TRYBB NADH-ubiquinone oxidoreductase chain 5 (NADH dehydrogenase subunit
5)
Length = 590
Score = 30.0 bits (66), Expect = 6.0
Identities = 29/97 (29%), Positives = 38/97 (39%), Gaps = 10/97 (10%)
Frame = -2
Query: 273 YLSSELYALLMVLQLTNTNA*LFTISSSIRFVCGSNSFTLRFVSWSFD----------LF 124
Y L ALLM+L N LF I F N F L F+ + F LF
Sbjct: 391 YCKDMLLALLMLLSFYNIIEFLFISIIFIFFTMIYNYFLLFFLMFVFKCFCLVDCLFLLF 450
Query: 123 SFACCSSLCFIRSFISRTNCFRFHQLLSILFVHVDIV 13
+ CC C I ++ +LSI F+ +D V
Sbjct: 451 DYECCLVYCLISLYMC---------ILSIFFI-IDFV 477
>sp|Q94165|HM34_CAEEL Homeobox protein ceh-34
Length = 256
Score = 29.6 bits (65), Expect = 7.8
Identities = 24/77 (31%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +2
Query: 170 LPQTNRMLLEIVKSQALVLV---NWRTINKAYSSDDK*TFRINHLVVNLEWKDGNSLSED 340
LPQ +++ ++K+QALV NW+ + K NH V+ W D +
Sbjct: 49 LPQCYQVMESVLKAQALVYFTTQNWKMLYKLLECSK--FSPHNHTVLQNLWLDAHYKEAA 106
Query: 341 KYKHRSQPAIVKTGYRI 391
K K R A+ K YRI
Sbjct: 107 KTKDRELGAVCK--YRI 121
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 75,470,434
Number of Sequences: 369166
Number of extensions: 1478851
Number of successful extensions: 4282
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4060
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4280
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5927776870
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)