Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_B23
(530 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9DFS6|IF4EA_BRARE Eukaryotic translation initiation fac... 65 8e-11
sp|P29338|IF4E_RABIT Eukaryotic translation initiation fact... 65 1e-10
sp|P06730|IF4E_HUMAN Eukaryotic translation initiation fact... 65 1e-10
sp|P63073|IF4E_MOUSE Eukaryotic translation initiation fact... 65 1e-10
sp|P48597|IF4E_XENLA Eukaryotic translation initiation fact... 64 2e-10
sp|Q9N0T5|IF4E_BOVIN Eukaryotic translation initiation fact... 64 3e-10
sp|O77210|IF4E_APLCA Eukaryotic translation initiation fact... 60 3e-09
sp|O45551|IF4E1_CAEEL Eukaryotic translation initiation fac... 55 8e-08
sp|Q21693|IF4E2_CAEEL Eukaryotic translation initiation fac... 55 1e-07
sp|O61955|IF4E3_CAEEL Eukaryotic translation initiation fac... 54 3e-07
>sp|Q9DFS6|IF4EA_BRARE Eukaryotic translation initiation factor 4E-1A (eIF4E-1A) (mRNA
cap-binding protein)
Length = 215
Score = 65.5 bits (158), Expect = 8e-11
Identities = 28/51 (54%), Positives = 37/51 (72%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWEDERNK GG L+T K++ + LD WLE L+ LVGE F+D SD++
Sbjct: 97 EPMWEDERNKRGGRWLITLSKQQRRADLDRFWLETLLCLVGEAFDDHSDDV 147
Score = 43.5 bits (101), Expect = 3e-04
Identities = 25/63 (39%), Positives = 35/63 (55%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIGKNV*KLSA--RRVLSSTLAHADAQKKSNS 330
CGAV N R GD+I++WTTD + +AI IG+ + +V+ +HAD KS S
Sbjct: 148 CGAVVNIRTKGDKIAIWTTDYENKDAIVHIGRVYKERLGVPPKVIIGYQSHADTATKSGS 207
Query: 331 KAK 339
K
Sbjct: 208 TTK 210
>sp|P29338|IF4E_RABIT Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 65.1 bits (157), Expect = 1e-10
Identities = 27/51 (52%), Positives = 39/51 (76%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWEDE+NK GG L+T +K++ S LD WLE L+ L+GE+F+D SD++
Sbjct: 99 EPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDV 149
Score = 46.6 bits (109), Expect = 4e-05
Identities = 23/63 (36%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIGKNV*KLSA--RRVLSSTLAHADAQKKSNS 330
CGAV N R GD+I++WTT+C + +A+ IG+ + +++ +HAD KS S
Sbjct: 150 CGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGS 209
Query: 331 KAK 339
K
Sbjct: 210 TTK 212
>sp|P06730|IF4E_HUMAN Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 65.1 bits (157), Expect = 1e-10
Identities = 27/51 (52%), Positives = 39/51 (76%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWEDE+NK GG L+T +K++ S LD WLE L+ L+GE+F+D SD++
Sbjct: 99 EPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDV 149
Score = 47.8 bits (112), Expect = 2e-05
Identities = 24/63 (38%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIGKNV*KLSA--RRVLSSTLAHADAQKKSNS 330
CGAV N R GD+I++WTT+C + EA+ IG+ + +++ +HAD KS S
Sbjct: 150 CGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGS 209
Query: 331 KAK 339
K
Sbjct: 210 TTK 212
>sp|P63073|IF4E_MOUSE Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
sp|P63074|IF4E_RAT Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 217
Score = 65.1 bits (157), Expect = 1e-10
Identities = 27/51 (52%), Positives = 39/51 (76%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWEDE+NK GG L+T +K++ S LD WLE L+ L+GE+F+D SD++
Sbjct: 99 EPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDV 149
Score = 46.6 bits (109), Expect = 4e-05
Identities = 23/63 (36%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIGKNV*KLSA--RRVLSSTLAHADAQKKSNS 330
CGAV N R GD+I++WTT+C + +A+ IG+ + +++ +HAD KS S
Sbjct: 150 CGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGS 209
Query: 331 KAK 339
K
Sbjct: 210 TTK 212
>sp|P48597|IF4E_XENLA Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 213
Score = 64.3 bits (155), Expect = 2e-10
Identities = 26/51 (50%), Positives = 39/51 (76%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWEDE+NK GG L+T +K++ + LD WLE LM L+GE+F++ SD++
Sbjct: 95 EPMWEDEKNKRGGRWLITLNKQQRRNDLDRFWLETLMCLIGESFDEHSDDV 145
Score = 43.5 bits (101), Expect = 3e-04
Identities = 26/64 (40%), Positives = 36/64 (56%), Gaps = 3/64 (4%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIG---KNV*KLSARRVLSSTLAHADAQKKSN 327
CGAV N R GD+I++WTT+ + +A+ IG K L A+ V+ +HAD KS
Sbjct: 146 CGAVVNVRAKGDKIAIWTTEFENKDAVTHIGRVYKERLGLPAKVVIGYQ-SHADTATKSG 204
Query: 328 SKAK 339
S K
Sbjct: 205 STTK 208
>sp|Q9N0T5|IF4E_BOVIN Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein)
Length = 217
Score = 63.5 bits (153), Expect = 3e-10
Identities = 26/51 (50%), Positives = 38/51 (74%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWEDE+NK GG L+T +K++ LD WLE L+ L+GE+F+D SD++
Sbjct: 99 EPMWEDEKNKRGGRWLITLNKQQRRGDLDRFWLETLLCLIGESFDDYSDDV 149
Score = 47.8 bits (112), Expect = 2e-05
Identities = 24/63 (38%), Positives = 36/63 (57%), Gaps = 2/63 (3%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIGKNV*KLSA--RRVLSSTLAHADAQKKSNS 330
CGAV N R GD+I++WTT+C + EA+ IG+ + +++ +HAD KS S
Sbjct: 150 CGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGS 209
Query: 331 KAK 339
K
Sbjct: 210 TTK 212
>sp|O77210|IF4E_APLCA Eukaryotic translation initiation factor 4E (eIF4E) (eIF-4E) (mRNA
cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 215
Score = 60.1 bits (144), Expect = 3e-09
Identities = 25/51 (49%), Positives = 36/51 (70%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
QPMWED +NK GG L+ +K + ++LD WLE L+ L+GE F++ S+EI
Sbjct: 97 QPMWEDAQNKKGGRWLINLNKTQRQTHLDDFWLETLLCLIGEGFDEHSEEI 147
Score = 40.8 bits (94), Expect = 0.002
Identities = 24/65 (36%), Positives = 34/65 (52%), Gaps = 2/65 (3%)
Frame = +1
Query: 151 KFCGAVFNKRG*GDRISVWTTDCSSAEAIKRIG-KNV*KLSA-RRVLSSTLAHADAQKKS 324
+ CGA N R GD++ +WT D EA K+IG K LS +++ AH+D K+
Sbjct: 146 EICGATVNIRNKGDKLGLWTRDAQKTEATKKIGIKLKESLSVPPKIVIGFQAHSDTAGKA 205
Query: 325 NSKAK 339
S K
Sbjct: 206 GSTVK 210
>sp|O45551|IF4E1_CAEEL Eukaryotic translation initiation factor 4E-1 (eIF4E-1) (eIF-4E-1)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 212
Score = 55.5 bits (132), Expect = 8e-08
Identities = 26/51 (50%), Positives = 31/51 (60%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
QPMWE N NGG L+ DK + +D+ WLEILM LVGE F D + I
Sbjct: 74 QPMWEVPENSNGGRWLIVIDKGKTPEMVDAIWLEILMALVGEQFGKDMESI 124
Score = 40.0 bits (92), Expect = 0.004
Identities = 18/31 (58%), Positives = 20/31 (64%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIG 249
CG V N RG G +ISVWT DC+ E RIG
Sbjct: 125 CGLVCNVRGKGSKISVWTKDCNDDETNMRIG 155
>sp|Q21693|IF4E2_CAEEL Eukaryotic translation initiation factor 4E-2 (eIF4E-2) (eIF-4E-2)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 228
Score = 54.7 bits (130), Expect = 1e-07
Identities = 22/51 (43%), Positives = 33/51 (64%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWE +N+NGG L+T +K +D+ W EILM ++GE F DD + +
Sbjct: 79 EPMWEVPQNQNGGRWLITIEKGRTPEIMDTIWTEILMAMIGEQFSDDIESL 129
Score = 36.6 bits (83), Expect = 0.039
Identities = 27/70 (38%), Positives = 34/70 (48%), Gaps = 9/70 (12%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIG------KNV*KLSARRVLSSTLA---HAD 309
CG V N RG G +ISVWTT+ + A RIG N + +R L L H
Sbjct: 130 CGIVCNVRGKGSKISVWTTNSADDGANLRIGGVLKQVLNNASMIHQRPLYDVLRYEDHES 189
Query: 310 AQKKSNSKAK 339
QKK++S K
Sbjct: 190 CQKKTSSGVK 199
>sp|O61955|IF4E3_CAEEL Eukaryotic translation initiation factor 4E-3 (eIF4E-3) (eIF-4E-3)
(mRNA cap-binding protein) (eIF-4F 25 kDa subunit)
Length = 248
Score = 53.5 bits (127), Expect = 3e-07
Identities = 24/51 (47%), Positives = 31/51 (60%)
Frame = +3
Query: 3 QPMWEDERNKNGGMCLLTFDKKENFSYLDSCWLEILMLLVGETFEDDSDEI 155
+PMWED N GG L+ DK+ LD WLE+LM +VGE F++ D I
Sbjct: 94 KPMWEDVNNVQGGRWLVVVDKQRRTQLLDHYWLELLMAIVGEQFDEYGDYI 144
Score = 35.0 bits (79), Expect = 0.11
Identities = 15/32 (46%), Positives = 21/32 (65%)
Frame = +1
Query: 157 CGAVFNKRG*GDRISVWTTDCSSAEAIKRIGK 252
CGAV N R GD++S+WT D + + RIG+
Sbjct: 145 CGAVVNVRQKGDKVSLWTRDATRDDVNLRIGQ 176
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,876,325
Number of Sequences: 369166
Number of extensions: 1009155
Number of successful extensions: 2683
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2599
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2666
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3600891075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)