Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_B21
(751 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5F356|PI52A_CHICK Phosphatidylinositol-4-phosphate 5-ki... 106 6e-23
sp|P48426|PI52A_HUMAN Phosphatidylinositol-4-phosphate 5-ki... 105 1e-22
sp|Q9R0I8|PI52A_RAT Phosphatidylinositol-4-phosphate 5-kina... 104 2e-22
sp|O13010|PI52A_PIG Phosphatidylinositol-4-phosphate 5-kina... 104 2e-22
sp|O70172|PI52A_MOUSE Phosphatidylinositol-4-phosphate 5-ki... 102 1e-21
sp|P78356|PI52B_HUMAN Phosphatidylinositol-4-phosphate 5-ki... 99 9e-21
sp|Q80XI4|PI52B_MOUSE Phosphatidylinositol-4-phosphate 5-ki... 98 3e-20
sp|O88377|PI52B_RAT Phosphatidylinositol-4-phosphate 5-kina... 98 3e-20
sp|O14986|PI51B_HUMAN Phosphatidylinositol-4-phosphate 5-ki... 65 1e-10
sp|Q9M1K2|PI5K4_ARATH Putative phosphatidylinositol-4-phosp... 61 3e-09
>sp|Q5F356|PI52A_CHICK Phosphatidylinositol-4-phosphate 5-kinase type II alpha
(1-phosphatidylinositol-4-phosphate 5-kinase 2-alpha)
(PtdIns(4)P-5-kinase isoform 2-alpha) (PIP5KII-alpha)
(Diphosphoinositide kinase 2-alpha)
Length = 405
Score = 106 bits (265), Expect = 6e-23
Identities = 67/189 (35%), Positives = 98/189 (51%), Gaps = 4/189 (2%)
Frame = +1
Query: 4 LFMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDE-ECAFSPGEDDDTVNGG 180
+F+ L+ D+EFL +M YSLLVGIHD V++ +E EC + GED+
Sbjct: 253 MFLEKLKKDVEFLAQLKLMDYSLLVGIHD------VERAEQEEVECEENDGEDEG----- 301
Query: 181 ADDVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPG-FIPTPYPAFTGEL 357
ES+G + +PPDSPG + + P GE
Sbjct: 302 ----------ESDGTHPIG--------------------TPPDSPGNTLNSSLPLAPGEF 331
Query: 358 DPHMELFGIKS--SRNPSTIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPEL 531
DP ++++GIKS S +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE
Sbjct: 332 DPAIDVYGIKSHESAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQ 391
Query: 532 YAKRFLEFV 558
Y+KRFL+F+
Sbjct: 392 YSKRFLDFI 400
>sp|P48426|PI52A_HUMAN Phosphatidylinositol-4-phosphate 5-kinase type II alpha
(1-phosphatidylinositol-4-phosphate 5-kinase 2-alpha)
(PtdIns(4)P-5-kinase isoform 2-alpha) (PIP5KII-alpha)
(Diphosphoinositide kinase 2-alpha) (PtdIns(4)P-5-kinase
B isoform) (PIP5KIII) (PtdIns(4)P-5-kinase C isoform)
Length = 406
Score = 105 bits (262), Expect = 1e-22
Identities = 65/189 (34%), Positives = 98/189 (51%), Gaps = 4/189 (2%)
Frame = +1
Query: 4 LFMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDE-ECAFSPGEDDDTVNGG 180
+F+ L+ D+EFL +M YSLLVGIHD V++ +E EC + GE++
Sbjct: 254 VFLEKLKKDVEFLAQLKLMDYSLLVGIHD------VERAEQEEVECEENDGEEEG----- 302
Query: 181 ADDVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPG-FIPTPYPAFTGEL 357
ES+G + +PPDSPG + + P GE
Sbjct: 303 ----------ESDGTHPVG--------------------TPPDSPGNTLNSSPPLAPGEF 332
Query: 358 DPHMELFGIKSSRNP--STIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPEL 531
DP+++++GIK N +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE
Sbjct: 333 DPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQ 392
Query: 532 YAKRFLEFV 558
Y+KRFL+F+
Sbjct: 393 YSKRFLDFI 401
>sp|Q9R0I8|PI52A_RAT Phosphatidylinositol-4-phosphate 5-kinase type II alpha
(1-phosphatidylinositol-4-phosphate 5-kinase 2-alpha)
(PtdIns(4)P-5-kinase isoform 2-alpha) (PIP5KII-alpha)
(Diphosphoinositide kinase 2-alpha) (PIPK2 alpha)
Length = 406
Score = 104 bits (260), Expect = 2e-22
Identities = 65/189 (34%), Positives = 98/189 (51%), Gaps = 4/189 (2%)
Frame = +1
Query: 4 LFMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDE-ECAFSPGEDDDTVNGG 180
+F+ L+ D+EFL +M YSLLVGIHD V++ +E EC + GE++
Sbjct: 254 IFLEKLKKDVEFLAQLKLMDYSLLVGIHD------VERAEQEEVECEENEGEEEG----- 302
Query: 181 ADDVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPG-FIPTPYPAFTGEL 357
ES+GA+ +PPDSPG + + P GE
Sbjct: 303 ----------ESDGAHPIG--------------------TPPDSPGNTLNSSPPLAPGEF 332
Query: 358 DPHMELFGIKSSRNP--STIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPEL 531
DP+++++ IK N +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE
Sbjct: 333 DPNIDVYAIKCHENAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQ 392
Query: 532 YAKRFLEFV 558
Y+KRFL+F+
Sbjct: 393 YSKRFLDFI 401
>sp|O13010|PI52A_PIG Phosphatidylinositol-4-phosphate 5-kinase type II alpha
(1-phosphatidylinositol-4-phosphate 5-kinase 2-alpha)
(PtdIns(4)P-5-kinase isoform 2-alpha) (PIP5KII-alpha)
(Diphosphoinositide kinase 2-alpha)
Length = 406
Score = 104 bits (260), Expect = 2e-22
Identities = 65/189 (34%), Positives = 97/189 (51%), Gaps = 4/189 (2%)
Frame = +1
Query: 4 LFMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDE-ECAFSPGEDDDTVNGG 180
+F+ L+ D+EFL +M YSLLVGIHD V++ +E EC + GE++
Sbjct: 254 VFLEKLKKDVEFLAQLKLMDYSLLVGIHD------VERAEQEEVECEENDGEEEG----- 302
Query: 181 ADDVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPG-FIPTPYPAFTGEL 357
ES+G + +PPDSPG + + P GE
Sbjct: 303 ----------ESDGTHPVG--------------------TPPDSPGNTLNSSPPLAPGEF 332
Query: 358 DPHMELFGIKSSRNP--STIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPEL 531
DP+++++GIK N +YF+ +++I T Y KK+ A K V+HG AEIS+V PE
Sbjct: 333 DPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKXVKHGAGAEISTVNPEQ 392
Query: 532 YAKRFLEFV 558
Y+KRFL+F+
Sbjct: 393 YSKRFLDFI 401
>sp|O70172|PI52A_MOUSE Phosphatidylinositol-4-phosphate 5-kinase type II alpha
(1-phosphatidylinositol-4-phosphate 5-kinase 2-alpha)
(PtdIns(4)P-5-kinase isoform 2-alpha) (PIP5KII-alpha)
(Diphosphoinositide kinase 2-alpha)
Length = 405
Score = 102 bits (254), Expect = 1e-21
Identities = 64/189 (33%), Positives = 95/189 (50%), Gaps = 4/189 (2%)
Frame = +1
Query: 4 LFMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDE-ECAFSPGEDDDTVNGG 180
+F+ L+ D+EFL +M YSLLVGIHD V++ +E EC + GE++ G
Sbjct: 254 IFLEKLKKDVEFLAQLKLMDYSLLVGIHD------VERAEQEEVECEENDGEEE-----G 302
Query: 181 ADDVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPG-FIPTPYPAFTGEL 357
D G +PPDSPG + + P GE
Sbjct: 303 ESDSTHPIG------------------------------TPPDSPGNTLNSSPPLAPGEF 332
Query: 358 DPHMELFGIKSSRNP--STIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPEL 531
DP+++++ IK N +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE
Sbjct: 333 DPNIDVYAIKCHENAPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQ 392
Query: 532 YAKRFLEFV 558
Y+KRFL+F+
Sbjct: 393 YSKRFLDFI 401
>sp|P78356|PI52B_HUMAN Phosphatidylinositol-4-phosphate 5-kinase type II beta
(1-phosphatidylinositol-4-phosphate 5-kinase 2-beta)
(PtdIns(4)P-5-kinase isoform 2-beta) (PIP5KII-beta)
(Diphosphoinositide kinase 2-beta)
Length = 416
Score = 99.4 bits (246), Expect = 9e-21
Identities = 65/189 (34%), Positives = 95/189 (50%), Gaps = 4/189 (2%)
Frame = +1
Query: 7 FMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDEECAFSPGEDDDTVNGGAD 186
F+ L+ D+EFL +M YSLLVGIHD VD+ +E ED++ N G
Sbjct: 260 FLEKLKRDVEFLAQLKIMDYSLLVGIHD------VDRAEQEEMEVEERAEDEECENDGV- 312
Query: 187 DVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPGFIPTPYPAF--TGELD 360
G N+ +PPDSPG + + +P F GE D
Sbjct: 313 -----------GGNLLCSYG-----------------TPPDSPGNLLS-FPRFFGPGEFD 343
Query: 361 PHMELFGIKS--SRNPSTIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPELY 534
P ++++ +KS S +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE Y
Sbjct: 344 PSVDVYAMKSHESSPKKEVYFMAIIDILTPYDTKKKAAHAAKTVKHGAGAEISTVNPEQY 403
Query: 535 AKRFLEFVN 561
+KRF EF++
Sbjct: 404 SKRFNEFMS 412
>sp|Q80XI4|PI52B_MOUSE Phosphatidylinositol-4-phosphate 5-kinase type II beta
(1-phosphatidylinositol-4-phosphate 5-kinase 2-beta)
(PtdIns(4)P-5-kinase isoform 2-beta) (PIP5KII-beta)
(Diphosphoinositide kinase 2-beta)
Length = 416
Score = 97.8 bits (242), Expect = 3e-20
Identities = 64/189 (33%), Positives = 97/189 (51%), Gaps = 4/189 (2%)
Frame = +1
Query: 7 FMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDEECAFSPGEDDDTVNGGAD 186
F+ L+ D+EFL +M YSLLVGIHD VD+ +E E+++ N G
Sbjct: 260 FLEKLKRDVEFLAQLKIMDYSLLVGIHD------VDRAEQEEMEVEERAEEEECENDG-- 311
Query: 187 DVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPGFIPTPYPAF--TGELD 360
+ G+L + +PPDSPG + + +P F GE D
Sbjct: 312 --------------------VGGSLLCSYG-------TPPDSPGNLLS-FPRFFGPGEFD 343
Query: 361 PHMELFGIKS--SRNPSTIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPELY 534
P ++++ +KS S +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE Y
Sbjct: 344 PSVDVYAMKSHESAPKKEVYFMAIIDILTPYDAKKKAAHAAKTVKHGAGAEISTVNPEQY 403
Query: 535 AKRFLEFVN 561
+KRF EF++
Sbjct: 404 SKRFNEFMS 412
>sp|O88377|PI52B_RAT Phosphatidylinositol-4-phosphate 5-kinase type II beta
(1-phosphatidylinositol-4-phosphate 5-kinase 2-beta)
(PtdIns(4)P-5-kinase isoform 2-beta) (PIP5KII-beta)
(Diphosphoinositide kinase 2-beta)
(Phosphatidylinositol-phosphate kinase IIgamma)
(PIPKIIgamma)
Length = 416
Score = 97.8 bits (242), Expect = 3e-20
Identities = 64/189 (33%), Positives = 96/189 (50%), Gaps = 4/189 (2%)
Frame = +1
Query: 7 FMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDEECAFSPGEDDDTVNGGAD 186
F+ L+ D+EFL +M YSLLVGIHD VD+ +E E+++ N G
Sbjct: 260 FLEKLKRDVEFLAQLKIMDYSLLVGIHD------VDRAEQEETEVEDRAEEEECENDG-- 311
Query: 187 DVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPGFIPTPYPAF--TGELD 360
+ G L + +PPDSPG + + +P F GE D
Sbjct: 312 --------------------VGGGLLCSYG-------TPPDSPGNLLS-FPRFFGPGEFD 343
Query: 361 PHMELFGIKS--SRNPSTIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPELY 534
P ++++ +KS S +YF+ +++I T Y KK+ A K+V+HG AEIS+V PE Y
Sbjct: 344 PSVDVYAMKSHESAPKKEVYFMAIIDILTPYDAKKKAAHAAKTVKHGAGAEISTVNPEQY 403
Query: 535 AKRFLEFVN 561
+KRF EF++
Sbjct: 404 SKRFNEFMS 412
>sp|O14986|PI51B_HUMAN Phosphatidylinositol-4-phosphate 5-kinase type I beta
(PtdIns(4)P-5-kinase beta) (PIP5KIbeta) (Type I
phosphatidylinositol-4-phosphate 5-kinase beta) (Protein
STM-7)
Length = 540
Score = 65.5 bits (158), Expect = 1e-10
Identities = 52/189 (27%), Positives = 80/189 (42%), Gaps = 2/189 (1%)
Frame = +1
Query: 10 MNMLQNDIEFLQNNNMMSYSLLVGIH--DPEMMGKVDKTPSDEECAFSPGEDDDTVNGGA 183
M LQ D L++ +M YSLL+GIH D + K ++TP + D G
Sbjct: 249 MKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNV---------PDAKRTGM 299
Query: 184 DDVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPGFIPTPYPAFTGELDP 363
V+ S ES +Q K GDG PD+ G IP
Sbjct: 300 QKVLYSTAMES--------------IQGPGKSGDGIITENPDTMGGIPA----------- 334
Query: 364 HMELFGIKSSRNPSTIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPELYAKR 543
KS R + F+G+++I Y + K+ ++K++ + + +S RP YA R
Sbjct: 335 -------KSHRGEKLLLFMGIIDILQSYRLMKKLEHSWKALVYDGDT-VSVHRPSFYADR 386
Query: 544 FLEFVNMAI 570
FL+F+N +
Sbjct: 387 FLKFMNSRV 395
>sp|Q9M1K2|PI5K4_ARATH Putative phosphatidylinositol-4-phosphate 5-kinase 4 (AtPIP5K4)
(1-phosphatidylinositol-4-phosphate kinase 4)
(PtdIns(4)P-5-kinase 4) (Diphosphoinositide kinase 4)
Length = 779
Score = 61.2 bits (147), Expect = 3e-09
Identities = 43/184 (23%), Positives = 74/184 (40%)
Frame = +1
Query: 7 FMNMLQNDIEFLQNNNMMSYSLLVGIHDPEMMGKVDKTPSDEECAFSPGEDDDTVNGGAD 186
F+ + D EFL+ +M YSLLVGIH E + PS ED+
Sbjct: 614 FIRQVDKDCEFLEQERIMDYSLLVGIHFREASVAGELIPSGARTPIGEFEDESAPRLSRA 673
Query: 187 DVVDSAGHESEGANVTSDSEITGALQSTIKQGDGACLSPPDSPGFIPTPYPAFTGELDPH 366
DV + A++ + + T+++ D C
Sbjct: 674 DVDQLLSDPTRWASIRLGGNMPARAERTMRRSD--C-----------------------E 708
Query: 367 MELFGIKSSRNPSTIYFLGVVNIFTKYGMKKRTAQTYKSVRHGHNAEISSVRPELYAKRF 546
+L G + + G+++I Y + K+ YKS+++ + IS+V P LY++RF
Sbjct: 709 FQLVGEPTGEYYEVVMIFGIIDILQDYDISKKLEHAYKSIQYDPTS-ISAVDPRLYSRRF 767
Query: 547 LEFV 558
+F+
Sbjct: 768 RDFI 771
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,900,743
Number of Sequences: 369166
Number of extensions: 1913597
Number of successful extensions: 5675
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5336
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5635
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6824907600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)