Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_002_A12
(677 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP... 233 3e-61
sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mi... 221 1e-57
sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mi... 221 1e-57
sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP... 221 1e-57
sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP... 207 2e-53
sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cyto... 206 5e-53
sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cy... 202 7e-52
sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cy... 196 4e-50
sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cy... 196 4e-50
sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cy... 195 1e-49
>sp|P29190|PPCK_HAECO Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 619
Score = 233 bits (594), Expect = 3e-61
Identities = 108/175 (61%), Positives = 134/175 (76%), Gaps = 2/175 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDLEKPGR 192
E +W HG+ GA LKSEATAAAE KGK +MHDPMAMRPFMGYNFG YLQHW+DL K GR
Sbjct: 442 ETRSWLHGIFTGACLKSEATAAAEHKGKTVMHDPMAMRPFMGYNFGHYLQHWIDLNKDGR 501
Query: 193 KMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGE-DIAEPSPIGLLPKHGTIN 369
K+P I+HVNWFR + KF+WPG+G+NIRVIDW+ RR++GE DI +PIG++PK G IN
Sbjct: 502 KVPKIYHVNWFRRDANNKFLWPGYGQNIRVIDWIVRRLDGEPDIGVDTPIGIVPKKGAIN 561
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIH 531
+GL D +WDE+ S+PK Y + D +E KFL EQ G DLP I E++ QE+RI+
Sbjct: 562 ASGLPDIQWDELMSVPKEYWTNDAKEIRKFLEEQVGPDLPKEIRAEMDAQEERIN 616
>sp|Q16822|PPCKM_HUMAN Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 221 bits (564), Expect = 1e-57
Identities = 98/177 (55%), Positives = 134/177 (75%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDLE-KPG 189
EAFNW+HGV +G +++SE+TAAAE KGK IMHDP AMRPF GYNFG YL+HWL +E + G
Sbjct: 464 EAFNWRHGVFVGRAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKG 523
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
++P IFHVNWFR ++ G F+WPGFGEN RV+DW+CRR+ GED A +PIGL+PK G ++
Sbjct: 524 AQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALD 583
Query: 370 VTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
++GL A ++FSLPK + ++V++ +L EQ +DLP ++ EL E+R+H M
Sbjct: 584 LSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM 640
>sp|Q8BH04|PPCKM_MOUSE Phosphoenolpyruvate carboxykinase, mitochondrial precursor [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-M)
Length = 640
Score = 221 bits (563), Expect = 1e-57
Identities = 96/177 (54%), Positives = 136/177 (76%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDLE-KPG 189
EAFNW+HGV +G++++SE+TAAAE KGK IMHDP AMRPF GYNFG+YL+HWL +E + G
Sbjct: 464 EAFNWRHGVFVGSAMRSESTAAAEHKGKTIMHDPFAMRPFFGYNFGRYLEHWLSMEGQKG 523
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
++P IFHVNWFR ++ G+F+WPGFGEN RV+DW+CRR+ GED A+ +PIGL+PK G ++
Sbjct: 524 ARLPRIFHVNWFRRDEAGRFLWPGFGENARVLDWICRRLEGEDSAQETPIGLVPKEGALD 583
Query: 370 VTGLDA-KWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
++GL A ++FS+PK + ++V++ +L EQ +DLP ++ EL E R+ M
Sbjct: 584 LSGLSAVDTSQLFSIPKDFWEQEVRDIRGYLTEQVNQDLPKEVLAELEALEGRVQKM 640
>sp|Q05893|PPCK_ASCSU Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 643
Score = 221 bits (563), Expect = 1e-57
Identities = 102/174 (58%), Positives = 131/174 (75%), Gaps = 2/174 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDLEKPGR 192
E+ +W HG+ +GA +KSEATAAAE GKQ+MHDPMAMRPFMGYNFG+Y++HW+ L +P
Sbjct: 465 ESRSWVHGIFVGACVKSEATAAAEHTGKQVMHDPMAMRPFMGYNFGRYMRHWMKLGQPPH 524
Query: 193 KMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGE-DIAEPSPIGLLPKHGTIN 369
K+P IFHVNWFR + KF+WPG+G+NIRVIDW+ RR +G+ IAE +PIG +PK GTIN
Sbjct: 525 KVPKIFHVNWFRQSADHKFLWPGYGDNIRVIDWILRRCSGDATIAEETPIGFIPKKGTIN 584
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRI 528
+ GL + WDE+ S+PK+Y ED+ ET F Q G DLP I +EL Q +RI
Sbjct: 585 LEGLPNVNWDELMSIPKSYWLEDMVETKTFFENQVGSDLPPEIAKELEAQTERI 638
>sp|P20007|PPCK_DROME Phosphoenolpyruvate carboxykinase [GTP] (Phosphoenolpyruvate
carboxylase) (PEPCK)
Length = 647
Score = 207 bits (528), Expect = 2e-53
Identities = 97/177 (54%), Positives = 128/177 (72%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDLEKPGR 192
EA +W HGV +GA+++SEATAAAE KGK IMHDP AMRPF GYNFG Y+ HWL +EK G
Sbjct: 472 EARDWTHGVFIGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGDYVAHWLSMEKRG- 530
Query: 193 KMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTINV 372
++P IFHVNWFR + +GKF+WPG+GEN RV++W+ RRVNGE S IG +P G +N+
Sbjct: 531 QVPKIFHVNWFRKSAEGKFMWPGYGENSRVLEWILRRVNGESCYVDSAIGHIPAEGALNL 590
Query: 373 TGLDAKWD--EMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
G+ K D E+FSLPK + S++V++ + Q G DLPA I Q+L+E R+ N+
Sbjct: 591 DGMKDKVDVKEIFSLPKEFWSQEVKDIRTYFESQVGADLPASIYQQLDELSSRVDNL 647
>sp|P07379|PPCKC_RAT Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 206 bits (524), Expect = 5e-53
Identities = 96/177 (54%), Positives = 127/177 (71%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDL-EKPG 189
EA +W+HGV +GA+++SEATAAAE KGK IMHDP AMRPF GYNFGKYL HWL + +P
Sbjct: 446 EALSWQHGVFVGAAMRSEATAAAEHKGKVIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPA 505
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
K+P IFHVNWFR + GKF+WPGFGEN RV++WM R+ GED A+ +PIG +PK +N
Sbjct: 506 AKLPKIFHVNWFRKDKNGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYVPKEDALN 565
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
+ GL D +E+F + K + ++V+E K+L +Q DLP I +EL ++RI M
Sbjct: 566 LKGLGDVNVEELFGISKEFWEKEVEEIDKYLEDQVNADLPYEIERELRALKQRISQM 622
>sp|Q9Z2V4|PPCKC_MOUSE Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 202 bits (514), Expect = 7e-52
Identities = 94/177 (53%), Positives = 126/177 (71%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDL-EKPG 189
EA +W+HGV +GA+++SEATAAAE KGK IMHDP AMRPF GYNFGKYL HWL + +P
Sbjct: 446 EALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPA 505
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
K+P IFHVNWFR + GKF+WPGFGEN RV++WM R+ GED A+ +PIG +PK +N
Sbjct: 506 AKLPKIFHVNWFRKDKDGKFLWPGFGENSRVLEWMFGRIEGEDSAKLTPIGYIPKENALN 565
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
+ GL +E+F + K + ++V+E ++L +Q DLP I +EL ++RI M
Sbjct: 566 LKGLGGVNVEELFGISKEFWEKEVEEIDRYLEDQVNTDLPYEIERELRALKQRISQM 622
>sp|P35558|PPCKC_HUMAN Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 196 bits (499), Expect = 4e-50
Identities = 91/177 (51%), Positives = 126/177 (71%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDL-EKPG 189
EA +W+HGV +GA+++SEATAAAE KGK IMHDP AMRPF GYNFGKYL HWL + + P
Sbjct: 446 EALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPA 505
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
K+P IFHVNWFR + +GKF+WPGFGEN RV++WM R++G+ + +PIG +PK +N
Sbjct: 506 AKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALN 565
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
+ GL E+FS+ K + ++V++ K+L +Q DLP I +E+ ++RI M
Sbjct: 566 LKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM 622
>sp|Q5R5J1|PPCKC_PONPY Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 196 bits (499), Expect = 4e-50
Identities = 92/177 (51%), Positives = 127/177 (71%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDL-EKPG 189
EA +W+HGV +GA+++SEATAAAE KGK IMHDP AMRPF GYNFGKYL HWL + + P
Sbjct: 446 EALSWQHGVFVGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAQHPA 505
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
K+P IFHVNWFR + +GKF+WPGFGEN RV++WM R++G+ A+ +PIG +PK +N
Sbjct: 506 AKLPKIFHVNWFRKDKEGKFLWPGFGENSRVLEWMFNRIDGKAGAKLTPIGYIPKEDALN 565
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
+ GL E+FS+ K + ++V++ K+L +Q DLP I +E+ ++RI M
Sbjct: 566 LKGLGHINVMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM 622
>sp|P05153|PPCKC_CHICK Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
(Phosphoenolpyruvate carboxylase) (PEPCK-C)
Length = 622
Score = 195 bits (495), Expect = 1e-49
Identities = 91/177 (51%), Positives = 123/177 (69%), Gaps = 2/177 (1%)
Frame = +1
Query: 13 EAFNWKHGVMLGASLKSEATAAAEFKGKQIMHDPMAMRPFMGYNFGKYLQHWLDL-EKPG 189
EAFNW+HGV +GA+++SEATAAAE KGK IMHDP AMRPF GYNFGKYL HWL + +P
Sbjct: 446 EAFNWQHGVFIGAAMRSEATAAAEHKGKIIMHDPFAMRPFFGYNFGKYLAHWLSMAHRPA 505
Query: 190 RKMPLIFHVNWFRLNDKGKFVWPGFGENIRVIDWMCRRVNGEDIAEPSPIGLLPKHGTIN 369
K+P IFHVNWFR + +GKF+WPG+GEN RV++WM R+ G+ A+ + IG +P +N
Sbjct: 506 AKLPRIFHVNWFRKDSQGKFLWPGYGENSRVLEWMFNRIQGKASAKSTAIGYIPADTALN 565
Query: 370 VTGL-DAKWDEMFSLPKAYLSEDVQETMKFLREQTGEDLPAGIIQELNEQEKRIHNM 537
+ GL D E+F++ K + ++V+E ++ Q DLP I +EL E RI +
Sbjct: 566 LKGLEDINLTELFNISKEFWEKEVEEIKQYFEGQVNADLPYEIERELLALEMRIKQL 622
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,700,677
Number of Sequences: 369166
Number of extensions: 1885888
Number of successful extensions: 4838
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4633
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4762
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5733423530
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)