Planarian EST Database


Dr_sW_002_A02

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_002_A02
         (657 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q15011|HERPU_HUMAN  Homocysteine-responsive endoplasmic r...    38   0.020
sp|Q9JJK5|HERPU_MOUSE  Homocysteine-responsive endoplasmic r...    38   0.020
sp|Q804S5|MIB1_BRARE  Ubiquitin ligase protein mib (Mind bom...    33   0.85 
sp|P34228|SEF1_YEAST  Suppressor protein SEF1                      32   1.1  
sp|P04933|MSP1_PLAFW  Merozoite surface protein 1 precursor ...    32   1.5  
sp|P04932|MSP1_PLAFK  Merozoite surface protein 1 precursor ...    32   1.5  
sp|Q98QQ2|Y309_MYCPU  Hypothetical UPF0348 protein MYPU_3090       32   1.9  
sp|Q86YT6|MIB1_HUMAN  Ubiquitin ligase protein MIB1 (Mind bo...    32   1.9  
sp|Q80SY4|MIB1_MOUSE  Ubiquitin ligase protein MIB1 (Mind bo...    32   1.9  
sp|Q98Q98|TRMD_MYCPU  tRNA (Guanine-N(1)-)-methyltransferase...    31   3.2  
>sp|Q15011|HERPU_HUMAN Homocysteine-responsive endoplasmic reticulum-resident
           ubiquitin-like domain member 1 protein (Methyl
           methanesulfonate (MMF)-inducible fragment protein 1)
          Length = 391

 Score = 38.1 bits (87), Expect = 0.020
 Identities = 22/62 (35%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
 Frame = +3

Query: 6   KPTVESQKVIYVGKLLKDDQILKNLF-EPESSTIVHFICQSSINGNEKKVDDVSKKPEIT 182
           +P  E Q++IY GKLL D Q L++L  + E   ++H +C  ++    K  +  +K  E T
Sbjct: 48  RPRPEDQRLIYSGKLLLDHQCLRDLLPKQEKRHVLHLVC--NVKSPSKMPEINAKVAEST 105

Query: 183 EE 188
           EE
Sbjct: 106 EE 107
>sp|Q9JJK5|HERPU_MOUSE Homocysteine-responsive endoplasmic reticulum-resident
           ubiquitin-like domain member 1 protein
          Length = 391

 Score = 38.1 bits (87), Expect = 0.020
 Identities = 24/73 (32%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
 Frame = +3

Query: 6   KPTVESQKVIYVGKLLKDDQILKNLF-EPESSTIVHFICQSSINGNEKKVDDVSKKPEIT 182
           +P  E Q++IY GKLL D Q L++L  + E   ++H +C  ++    K  +  +K  E T
Sbjct: 48  RPRPEDQRLIYSGKLLLDHQCLQDLLPKQEKRHVLHLVC--NVKNPSKMPETSTKGAEST 105

Query: 183 EEKRPDLYKEYQN 221
           E+  PD   + Q+
Sbjct: 106 EQ--PDNSNQTQH 116
>sp|Q804S5|MIB1_BRARE Ubiquitin ligase protein mib (Mind bomb protein)
          Length = 1030

 Score = 32.7 bits (73), Expect = 0.85
 Identities = 16/40 (40%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
 Frame = +3

Query: 63  QILKNLFEP-ESSTIVHFICQSSINGNEKKVDDVSKKPEI 179
           Q+LK LFE  ES  I   + +++ NG+  KV+D+ K+P++
Sbjct: 417 QLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDV 456
>sp|P34228|SEF1_YEAST Suppressor protein SEF1
          Length = 1057

 Score = 32.3 bits (72), Expect = 1.1
 Identities = 20/56 (35%), Positives = 26/56 (46%)
 Frame = -2

Query: 638 QQQQSKSHQNIEIMQNISRFDLSIILIFSSVSHQPSKCCSHHVLHSILCWRSINSP 471
           +QQQ + HQ     Q IS  D S       ++H+P   C+H   H I C  S N P
Sbjct: 24  EQQQLQQHQFPSKKQRISHHDDS-----HQINHRPVTSCTHCRQHKIKCDASQNFP 74
>sp|P04933|MSP1_PLAFW Merozoite surface protein 1 precursor (Merozoite surface antigens)
           (PMMSA) (P195)
          Length = 1639

 Score = 32.0 bits (71), Expect = 1.5
 Identities = 15/63 (23%), Positives = 31/63 (49%)
 Frame = +3

Query: 120 QSSINGNEKKVDDVSKKPEITEEKRPDLYKEYQNYVNNYYASTSQSFAAINHYQQMMWMN 299
           +  I   +KK++   K  E   +   D+ KEY+  +N  Y S   +   + ++++MM   
Sbjct: 457 KEKIKIEKKKIESDKKSYEDRSKSLNDITKEYEKLLNEIYDSKFNNNIDLTNFEKMMGKR 516

Query: 300 HAY 308
           ++Y
Sbjct: 517 YSY 519
>sp|P04932|MSP1_PLAFK Merozoite surface protein 1 precursor (Merozoite surface antigens)
           (PMMSA) (P190)
          Length = 1630

 Score = 32.0 bits (71), Expect = 1.5
 Identities = 15/63 (23%), Positives = 31/63 (49%)
 Frame = +3

Query: 120 QSSINGNEKKVDDVSKKPEITEEKRPDLYKEYQNYVNNYYASTSQSFAAINHYQQMMWMN 299
           +  I   +KK++   K  E   +   D+ KEY+  +N  Y S   +   + ++++MM   
Sbjct: 448 KEKIKIEKKKIESDKKSYEDRSKSLNDITKEYEKLLNEIYDSKFNNNIDLTNFEKMMGKR 507

Query: 300 HAY 308
           ++Y
Sbjct: 508 YSY 510
>sp|Q98QQ2|Y309_MYCPU Hypothetical UPF0348 protein MYPU_3090
          Length = 298

 Score = 31.6 bits (70), Expect = 1.9
 Identities = 18/69 (26%), Positives = 35/69 (50%), Gaps = 4/69 (5%)
 Frame = +3

Query: 156 DVSKKPEITEEKRP----DLYKEYQNYVNNYYASTSQSFAAINHYQQMMWMNHAYMQSYA 323
           DVSK   +  EK+P    DLY ++Q  + N  A   + F  ++   + ++  H   +SY 
Sbjct: 210 DVSKYTPMIFEKKPIRIEDLYFDFQTIIFNKTAQELRQFKLVSEGIENLFKKHINEKSYD 269

Query: 324 SYCAQFSTR 350
            + A+ +++
Sbjct: 270 DFVAKVNSK 278
>sp|Q86YT6|MIB1_HUMAN Ubiquitin ligase protein MIB1 (Mind bomb homolog 1)
           (DAPK-interacting protein 1) (DIP-1) (Zinc finger ZZ
           type with ankyrin repeat domain protein 2)
          Length = 1006

 Score = 31.6 bits (70), Expect = 1.9
 Identities = 15/40 (37%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
 Frame = +3

Query: 63  QILKNLFEP-ESSTIVHFICQSSINGNEKKVDDVSKKPEI 179
           Q+LK LFE  ES  +   + +++ NG+  KV+D+ K+P++
Sbjct: 417 QLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDV 456
>sp|Q80SY4|MIB1_MOUSE Ubiquitin ligase protein MIB1 (Mind bomb homolog 1)
           (DAPK-interacting protein 1) (DIP-1)
          Length = 1006

 Score = 31.6 bits (70), Expect = 1.9
 Identities = 15/40 (37%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
 Frame = +3

Query: 63  QILKNLFEP-ESSTIVHFICQSSINGNEKKVDDVSKKPEI 179
           Q+LK LFE  ES  +   + +++ NG+  KV+D+ K+P++
Sbjct: 417 QLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDV 456
>sp|Q98Q98|TRMD_MYCPU tRNA (Guanine-N(1)-)-methyltransferase (M1G-methyltransferase)
           (tRNA [GM37] methyltransferase)
          Length = 225

 Score = 30.8 bits (68), Expect = 3.2
 Identities = 13/33 (39%), Positives = 23/33 (69%), Gaps = 2/33 (6%)
 Frame = +3

Query: 129 INGNEKKVDDVSKKPEITEEK--RPDLYKEYQN 221
           +NGN K+++   K+ +I + K  RPDLY+ ++N
Sbjct: 191 LNGNHKEIEKWRKQKQIEKTKINRPDLYERFKN 223
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,704,806
Number of Sequences: 369166
Number of extensions: 1020793
Number of successful extensions: 3390
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3283
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3387
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5462583840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)