Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_P22
(879 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P24757|RPO4_VACCV DNA-directed RNA polymerase 35 kDa pol... 33 1.0
sp|P33812|RPO4_VARV DNA-directed RNA polymerase 35 kDa poly... 33 1.4
sp|Q5GRT1|ATPG_WOLTR ATP synthase gamma chain (ATP synthase... 33 1.4
sp|O57233|RPO4_VACCA DNA-directed RNA polymerase 35 kDa pol... 32 1.8
sp|Q8TLC0|YV17_METAC Hypothetical UPF0228 protein MA3117 32 1.8
sp|P06548|BLA3_BACCE Beta-lactamase III precursor 32 2.3
sp|Q6RZF4|RPO4_RABPU DNA-directed RNA polymerase 35 kDa pol... 32 3.0
sp|Q77TH6|RPO4_VACCT DNA-directed RNA polymerase 35 kDa pol... 32 3.0
sp|Q9Y4L1|OXRP_HUMAN 150 kDa oxygen-regulated protein precu... 32 3.0
sp|Q42434|BIP_SPIOL Luminal binding protein precursor (BiP)... 31 4.0
>sp|P24757|RPO4_VACCV DNA-directed RNA polymerase 35 kDa polypeptide
Length = 305
Score = 33.1 bits (74), Expect = 1.0
Identities = 39/171 (22%), Positives = 69/171 (40%), Gaps = 21/171 (12%)
Frame = +3
Query: 390 ERKFEKWNNYSKKHLKDHFFCDKLFRAVYLLNLYWRRMESFYHLLEKFGKNKQHIAKLRN 569
+R + +NY + D++ DK +V +++ E+ H+ +++ +L
Sbjct: 84 KRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIEL-- 141
Query: 570 CKSIFSFGCGAAPEIAAFLHFYKVKLGYRNYNNLSTIKGVD------------------- 692
FS G +I + L F V++ R YNN+ TI D
Sbjct: 142 --IAFSSGTVINEDIVSKLTFLDVEVFNREYNNVKTIIDPDFVFRSPFIVISPMGKLTFF 199
Query: 693 -TIKDWECYISVIEDVFKINFLFKNEDIN-ENFMLNFENVDLLMMSYSYES 839
+ W + S ++D+ I+FL N N M+ N D + SY+ ES
Sbjct: 200 VEVYSWFDFKSCLKDI--IDFLEGALIANIHNHMIKVGNCDETVSSYNPES 248
>sp|P33812|RPO4_VARV DNA-directed RNA polymerase 35 kDa polypeptide
Length = 305
Score = 32.7 bits (73), Expect = 1.4
Identities = 24/103 (23%), Positives = 46/103 (44%)
Frame = +3
Query: 390 ERKFEKWNNYSKKHLKDHFFCDKLFRAVYLLNLYWRRMESFYHLLEKFGKNKQHIAKLRN 569
+R + +NY + D++ DK +V +++ E+ H+ +++ +L
Sbjct: 84 KRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIEL-- 141
Query: 570 CKSIFSFGCGAAPEIAAFLHFYKVKLGYRNYNNLSTIKGVDTI 698
FS G +I + L F V++ R YNN+ TI D +
Sbjct: 142 --IAFSSGTVINEDIVSKLTFLDVEVFNREYNNVKTIMNPDFV 182
>sp|Q5GRT1|ATPG_WOLTR ATP synthase gamma chain (ATP synthase F1 sector gamma subunit)
Length = 290
Score = 32.7 bits (73), Expect = 1.4
Identities = 27/93 (29%), Positives = 44/93 (47%), Gaps = 7/93 (7%)
Frame = +3
Query: 534 GKNK---QHIAKLRNCKSIFSFGCGAAPEIAAFLHFYKVKLGYRNYNNLSTIKGV-DTIK 701
GKN+ + I K+ N K I A + + ++K+ Y + N T K + +TIK
Sbjct: 127 GKNRFDSKSILKIENSKGITLKRVEALVDSMDLSKYDRIKVFYNKFYNTFTQKPMLETIK 186
Query: 702 DWECYISVIEDVF---KINFLFKNEDINENFML 791
W S+I+D I++ +K E N F+L
Sbjct: 187 PWSKSSSLIDDYLTNSTIDYNYKYEPQNTEFIL 219
>sp|O57233|RPO4_VACCA DNA-directed RNA polymerase 35 kDa polypeptide
Length = 305
Score = 32.3 bits (72), Expect = 1.8
Identities = 39/171 (22%), Positives = 68/171 (39%), Gaps = 21/171 (12%)
Frame = +3
Query: 390 ERKFEKWNNYSKKHLKDHFFCDKLFRAVYLLNLYWRRMESFYHLLEKFGKNKQHIAKLRN 569
+R + +NY + D++ DK +V +++ E+ H+ +++ +L
Sbjct: 84 KRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIEL-- 141
Query: 570 CKSIFSFGCGAAPEIAAFLHFYKVKLGYRNYNNLSTIKGVD------------------- 692
FS G +I + L F V++ R YNN+ TI D
Sbjct: 142 --IAFSSGTVINEDIVSKLTFLDVEVFNREYNNVKTIIDPDFVFRSPFIVISPMGKLTFF 199
Query: 693 -TIKDWECYISVIEDVFKINFLFKNEDIN-ENFMLNFENVDLLMMSYSYES 839
+ W + S +D+ I+FL N N M+ N D + SY+ ES
Sbjct: 200 VEVYSWFDFKSCFKDI--IDFLEGALIANIHNHMIKVGNCDETVSSYNPES 248
>sp|Q8TLC0|YV17_METAC Hypothetical UPF0228 protein MA3117
Length = 181
Score = 32.3 bits (72), Expect = 1.8
Identities = 23/76 (30%), Positives = 37/76 (48%)
Frame = +3
Query: 606 PEIAAFLHFYKVKLGYRNYNNLSTIKGVDTIKDWECYISVIEDVFKINFLFKNEDINENF 785
PE+ A L Y + + Y N + +GV Y+ V ED KIN L KNE++
Sbjct: 46 PEVTAILENYDIPVNYTIDYNSNIGRGV-------YYVKVDED--KINELRKNENLISEI 96
Query: 786 MLNFENVDLLMMSYSY 833
L N +++++S +
Sbjct: 97 ELKKGNYNIIILSEEF 112
>sp|P06548|BLA3_BACCE Beta-lactamase III precursor
Length = 316
Score = 32.0 bits (71), Expect = 2.3
Identities = 19/60 (31%), Positives = 27/60 (45%)
Frame = +3
Query: 12 SKKLLQNTEIKDENKENGMKNHQDEELINCLTAIAGNDLSVNETEEYMTSDEFIFENIDK 191
+K+ Q ++K ENK N ++E N I D S N+T Y D F F + K
Sbjct: 40 NKQTNQTNQVKQENKRNHAFAKLEKEY-NAKLGIYALDTSTNQTVAYHADDRFAFASTSK 98
>sp|Q6RZF4|RPO4_RABPU DNA-directed RNA polymerase 35 kDa polypeptide
Length = 305
Score = 31.6 bits (70), Expect = 3.0
Identities = 24/103 (23%), Positives = 46/103 (44%)
Frame = +3
Query: 390 ERKFEKWNNYSKKHLKDHFFCDKLFRAVYLLNLYWRRMESFYHLLEKFGKNKQHIAKLRN 569
+R + +NY + D++ DK +V +++ E+ H+ +++ +L
Sbjct: 84 KRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIEL-- 141
Query: 570 CKSIFSFGCGAAPEIAAFLHFYKVKLGYRNYNNLSTIKGVDTI 698
FS G +I + L F V++ R YNN+ TI D +
Sbjct: 142 --IAFSSGTVINEDIVSKLTFLDVEVFNREYNNVKTIIDPDFV 182
>sp|Q77TH6|RPO4_VACCT DNA-directed RNA polymerase 35 kDa polypeptide
sp|P21087|RPO4_VACCC DNA-directed RNA polymerase 35 kDa polypeptide
Length = 305
Score = 31.6 bits (70), Expect = 3.0
Identities = 24/103 (23%), Positives = 46/103 (44%)
Frame = +3
Query: 390 ERKFEKWNNYSKKHLKDHFFCDKLFRAVYLLNLYWRRMESFYHLLEKFGKNKQHIAKLRN 569
+R + +NY + D++ DK +V +++ E+ H+ +++ +L
Sbjct: 84 KRSQSERDNYITLYDFDYYIIDKDTNSVTMVDKPTELKETLLHVFQEYRLKSSQTIEL-- 141
Query: 570 CKSIFSFGCGAAPEIAAFLHFYKVKLGYRNYNNLSTIKGVDTI 698
FS G +I + L F V++ R YNN+ TI D +
Sbjct: 142 --IAFSSGTVINEDIVSKLTFLDVEVFNREYNNVKTIIDPDFV 182
>sp|Q9Y4L1|OXRP_HUMAN 150 kDa oxygen-regulated protein precursor (Orp150) (Hypoxia
up-regulated 1)
Length = 999
Score = 31.6 bits (70), Expect = 3.0
Identities = 29/94 (30%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
Frame = +3
Query: 333 EKLIIDIKQKSSEIKWKNAERKFEKWNNYSKKHLKDHFFCDKLFRAVYLLNLYWRRMES- 509
E L + Q + +K K F+K+ +Y K +K HF D+ V L+ R+ES
Sbjct: 506 EDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDE--SGVLSLD----RVESV 559
Query: 510 FYHLLEKFGKNKQHIAKLRNCKSIFSFGCGAAPE 611
F L+E + + + KL N S FG G P+
Sbjct: 560 FETLVEDSAEEESTLTKLGNTISSL-FGGGTTPD 592
>sp|Q42434|BIP_SPIOL Luminal binding protein precursor (BiP) (78 kDa glucose-regulated
protein homolog) (GRP 78)
Length = 668
Score = 31.2 bits (69), Expect = 4.0
Identities = 21/68 (30%), Positives = 33/68 (48%), Gaps = 5/68 (7%)
Frame = +3
Query: 288 IFNEKTDISKCVKLIEKLIIDIKQK-----SSEIKWKNAERKFEKWNNYSKKHLKDHFFC 452
I+N K IS KL +KL D K+K ++W + + EK +Y +K + C
Sbjct: 578 IYNMKNQISDADKLADKLESDEKEKIEGAVKEALEWLDDNQSAEK-EDYDEKLKEVEAVC 636
Query: 453 DKLFRAVY 476
+ + AVY
Sbjct: 637 NPIITAVY 644
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,307,107
Number of Sequences: 369166
Number of extensions: 1434753
Number of successful extensions: 4978
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4696
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4976
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8742211660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)