Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_N24
(864 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q640G7|ATG4B_XENLA Cysteine protease ATG4B (Autophagy-re... 145 2e-34
sp|Q6PZ02|ATG4B_CHICK Cysteine protease ATG4B (Autophagy-re... 144 3e-34
sp|Q8BGE6|ATG4B_MOUSE Cysteine protease ATG4B (Autophagy-re... 144 4e-34
sp|Q9Y4P1|ATG4B_HUMAN Cysteine protease ATG4B (Autophagy-re... 143 5e-34
sp|Q6PZ03|ATG4B_BOVIN Cysteine protease ATG4B (Autophagy-re... 139 8e-33
sp|Q8C9S8|ATG4A_MOUSE Cysteine protease ATG4A (Autophagy-re... 137 5e-32
sp|Q6PZ05|ATG4A_BOVIN Cysteine protease ATG4A (Autophagy-re... 136 7e-32
sp|Q6GPU1|ATG4A_XENLA Cysteine protease ATG4A (Autophagy-re... 135 1e-31
sp|Q8WYN0|ATG4A_HUMAN Cysteine protease ATG4A (Autophagy-re... 134 3e-31
sp|Q5R699|ATG4A_PONPY Cysteine protease ATG4A (Autophagy-re... 133 6e-31
>sp|Q640G7|ATG4B_XENLA Cysteine protease ATG4B (Autophagy-related protein 4 homolog B)
Length = 384
Score = 145 bits (365), Expect = 2e-34
Identities = 78/187 (41%), Positives = 122/187 (65%), Gaps = 1/187 (0%)
Frame = +3
Query: 132 SKTQWRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDL 311
S QW+PL+L+IP+RLGL+E+NE Y+ LK F +PQ +G+IGGRP+ A +FIG VG++L
Sbjct: 206 SCAQWKPLVLLIPLRLGLSEINEAYIETLKHCFMVPQSLGVIGGRPNSAHYFIGYVGDEL 265
Query: 312 IYLDPHEPQAVVPLKTYCQVLDDESYHCT-KWSKMPIIKVDPSLAIGFVCTSEDSFITLC 488
IYLDPH Q V + C ++DES+HC +M + ++DPS+A+GF C+S++ F C
Sbjct: 266 IYLDPHTTQLSVE-PSDCSFIEDESFHCQHPPCRMHVSEIDPSIAVGFFCSSQEDFEDWC 324
Query: 489 EQLKRKILIPAGNYPMFEIHQKRPYYFPPLYNCNISVNGTDDSAEEFELI*NAIFIHSND 668
+ +K K+ + G PMFE+ + P + L N ++ +N T DS++ L + F ++
Sbjct: 325 QHIK-KLSLSGGALPMFEVVDQLPLH---LSNPDV-LNLTPDSSDADRL--DRFFDSEDE 377
Query: 669 LFSIVTL 689
F I++L
Sbjct: 378 EFEILSL 384
>sp|Q6PZ02|ATG4B_CHICK Cysteine protease ATG4B (Autophagy-related protein 4 homolog B)
(Autophagy-related cysteine endopeptidase 2B)
(Autophagin-2B) (cAut2B)
Length = 393
Score = 144 bits (363), Expect = 3e-34
Identities = 76/165 (46%), Positives = 110/165 (66%), Gaps = 3/165 (1%)
Frame = +3
Query: 144 WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDLIYLD 323
W+PL+L+IP+RLGL E+NE Y+ LK F +PQ +G+IGG+P+ A +FIG VGE+LIYLD
Sbjct: 219 WKPLVLLIPLRLGLTEINEAYIETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLD 278
Query: 324 PHEPQ-AVVPLKTYCQVLDDESYHCT-KWSKMPIIKVDPSLAIGFVCTSEDSFITLCEQL 497
PH Q AV P + C L DES+HC +M I ++DPS+A+GF C +E+ F C Q+
Sbjct: 279 PHTTQPAVEPSDSGC--LPDESFHCQHPPCRMSIAELDPSIAVGFFCHTEEDFNDWCHQI 336
Query: 498 KRKILIPAGNYPMFEIHQKRPYYFPPLYNCNISVNGTD-DSAEEF 629
K K+ + G PMFE+ +++P +F N++ + +D D E F
Sbjct: 337 K-KLSLVRGALPMFELVERQPSHFSNPDVLNLTPDSSDADRLERF 380
>sp|Q8BGE6|ATG4B_MOUSE Cysteine protease ATG4B (Autophagy-related protein 4 homolog B)
(Autophagin-1) (Autophagy-related cysteine endopeptidase
1) (AUT-like 1 cysteine endopeptidase)
Length = 393
Score = 144 bits (362), Expect = 4e-34
Identities = 73/157 (46%), Positives = 106/157 (67%), Gaps = 1/157 (0%)
Frame = +3
Query: 144 WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDLIYLD 323
WRPL+L+IP+RLGL ++NE YV LK F +PQ +G+IGG+P+ A +FIG VGE+LIYLD
Sbjct: 219 WRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLD 278
Query: 324 PHEPQAVVPLKTYCQVLDDESYHCT-KWSKMPIIKVDPSLAIGFVCTSEDSFITLCEQLK 500
PH Q V L C + DES+HC S+M I ++DPS+A+GF C +E+ F C+Q+K
Sbjct: 279 PHTTQPAVELTDSC-FIPDESFHCQHPPSRMGIGELDPSIAVGFFCKTEEDFNDWCQQVK 337
Query: 501 RKILIPAGNYPMFEIHQKRPYYFPPLYNCNISVNGTD 611
K+ G PMFE+ +++P + N+S++ +D
Sbjct: 338 -KLSQLGGALPMFELVEQQPSHLACQDVLNLSLDSSD 373
>sp|Q9Y4P1|ATG4B_HUMAN Cysteine protease ATG4B (Autophagy-related protein 4 homolog B)
(hAPG4B) (Autophagin-1) (Autophagy-related cysteine
endopeptidase 1) (AUT-like 1 cysteine endopeptidase)
Length = 393
Score = 143 bits (361), Expect = 5e-34
Identities = 72/157 (45%), Positives = 105/157 (66%), Gaps = 1/157 (0%)
Frame = +3
Query: 144 WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDLIYLD 323
WRPL+L+IP+RLGL ++NE YV LK F +PQ +G+IGG+P+ A +FIG VGE+LIYLD
Sbjct: 219 WRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLD 278
Query: 324 PHEPQAVVPLKTYCQVLDDESYHCT-KWSKMPIIKVDPSLAIGFVCTSEDSFITLCEQLK 500
PH Q V C + DES+HC +M I ++DPS+A+GF C +ED F C+Q+K
Sbjct: 279 PHTTQPAVEPTDGC-FIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVK 337
Query: 501 RKILIPAGNYPMFEIHQKRPYYFPPLYNCNISVNGTD 611
K+ + G PMFE+ +++P + N+S++ +D
Sbjct: 338 -KLSLLGGALPMFELVEQQPSHLACPDVLNLSLDSSD 373
>sp|Q6PZ03|ATG4B_BOVIN Cysteine protease ATG4B (Autophagy-related protein 4 homolog B)
(Autophagy-related cysteine endopeptidase 2B)
(Autophagin-2B) (bAut2B)
Length = 393
Score = 139 bits (351), Expect = 8e-33
Identities = 77/183 (42%), Positives = 114/183 (62%), Gaps = 1/183 (0%)
Frame = +3
Query: 144 WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDLIYLD 323
WRPL+L+IP+RLGL ++N Y LK F++PQ +G+IGG+P+ A +FIG VGE+LIYLD
Sbjct: 219 WRPLVLLIPLRLGLADVNAAYAGTLKHCFRMPQSLGVIGGKPNSAHYFIGYVGEELIYLD 278
Query: 324 PHEPQAVVPLKTYCQVLDDESYHCT-KWSKMPIIKVDPSLAIGFVCTSEDSFITLCEQLK 500
PH Q V C V DES+HC +M I ++DPS+A+GF C +ED F C+Q+
Sbjct: 279 PHTTQPAVAAADRCPV-PDESFHCQHPPGRMSIAELDPSIAVGFFCETEDDFNDWCQQVS 337
Query: 501 RKILIPAGNYPMFEIHQKRPYYFPPLYNCNISVNGTDDSAEEFELI*NAIFIHSNDLFSI 680
K+ + G PMFE+ +++P + N+S++ +D AE E F ++ F I
Sbjct: 338 -KLSLLGGALPMFELVEQQPSHLACPDVLNLSLDSSD--AERLE----RFFDSEDEDFEI 390
Query: 681 VTL 689
++L
Sbjct: 391 LSL 393
>sp|Q8C9S8|ATG4A_MOUSE Cysteine protease ATG4A (Autophagy-related protein 4 homolog A)
(Autophagin-2) (Autophagy-related cysteine endopeptidase
2) (AUT-like 2 cysteine endopeptidase)
Length = 396
Score = 137 bits (344), Expect = 5e-32
Identities = 68/173 (39%), Positives = 112/173 (64%), Gaps = 9/173 (5%)
Frame = +3
Query: 144 WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDLIYLD 323
W+PLLLI+P+RLG+N++N YV A K+ F++PQ +G +GG+P++A +FIG +G++LI+LD
Sbjct: 217 WKPLLLIVPLRLGINQINPVYVEAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLD 276
Query: 324 PHEPQAVVPLKTYCQVLDDESYHCTKW-SKMPIIKVDPSLAIGFVCTSEDSFITLCEQLK 500
PH Q V ++ ++DD+++HC + +M I+ +DPS+A+GF C E F C ++
Sbjct: 277 PHTTQTFVDIEE-SGLVDDQTFHCLQSPQRMSILNLDPSVALGFFCKEEKDFDNWCSLVQ 335
Query: 501 RKILIPAGNYPMFEIHQKRPYYFPPLY---NCNISVNGTD-----DSAEEFEL 635
++IL N MFE+ QK P ++PP ++ G + + E+FEL
Sbjct: 336 KEIL--KENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIESTEQLEDFEL 386
>sp|Q6PZ05|ATG4A_BOVIN Cysteine protease ATG4A (Autophagy-related protein 4 homolog A)
(Autophagy-related cysteine endopeptidase 2A)
(Autophagin-2A) (bAut2A)
Length = 398
Score = 136 bits (343), Expect = 7e-32
Identities = 70/175 (40%), Positives = 112/175 (64%), Gaps = 6/175 (3%)
Frame = +3
Query: 126 SSSKTQWRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGE 305
S+ T W+PLLLI+P+RLG+N++N YV A K+ F++PQ +G +GG+P++A +FIG +G+
Sbjct: 214 SACCTAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGD 273
Query: 306 DLIYLDPHEPQAVVPLKTYCQVLDDESYHCTK-WSKMPIIKVDPSLAIGFVCTSEDSFIT 482
+LI+LDPH Q V + DD+++HC + +M I+ +DPS+A+GF C E F +
Sbjct: 274 ELIFLDPHTTQTFVDTEEN-GTADDQTFHCLQPPQRMNILNLDPSVALGFFCKEEKDFDS 332
Query: 483 LCEQLKRKILIPAGNYPMFEIHQKRPYYFPPLY---NCNISVNGTD--DSAEEFE 632
C ++++IL N MFE+ QK P ++PP ++ G + DS E+ E
Sbjct: 333 WCSLVQKEIL--KENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLE 385
>sp|Q6GPU1|ATG4A_XENLA Cysteine protease ATG4A (Autophagy-related protein 4 homolog A)
Length = 397
Score = 135 bits (341), Expect = 1e-31
Identities = 69/178 (38%), Positives = 108/178 (60%), Gaps = 13/178 (7%)
Frame = +3
Query: 144 WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGRPSHALWFIGQVGEDLIYLD 323
WRPLLL++P+RLG+N +N YV A K F++PQ +G +GG+P+HA +FIG G+++IYLD
Sbjct: 220 WRPLLLVVPLRLGINHINPVYVDAFKACFKMPQSLGALGGKPNHAYYFIGFSGDEIIYLD 279
Query: 324 PHEPQAVVPLKTYCQVLDDESYHCTKW-SKMPIIKVDPSLAIGFVCTSEDSFITLCEQLK 500
PH Q V + + D++YHC K + M ++ +DPS+A+GF C E+ F CE ++
Sbjct: 280 PHTTQTFVDTEE-AGTVQDQTYHCQKGPNSMKVLNLDPSVALGFFCKDENDFNNWCEVIE 338
Query: 501 RKILIPAGNYPMFEIHQKRPYYFPPLY---NCNISVNGTD---------DSAEEFELI 638
++IL + MFE+ K P ++PP ++ G + D EEFE++
Sbjct: 339 KEIL-KHQSLRMFELTPKHPPHWPPFIPPTKPEVTTTGAELIESTDKLFDVEEEFEIL 395
>sp|Q8WYN0|ATG4A_HUMAN Cysteine protease ATG4A (Autophagy-related protein 4 homolog A)
(hAPG4A) (Autophagin-2) (Autophagy-related cysteine
endopeptidase 2) (AUT-like 2 cysteine endopeptidase)
Length = 398
Score = 134 bits (337), Expect = 3e-31
Identities = 72/190 (37%), Positives = 119/190 (62%), Gaps = 14/190 (7%)
Frame = +3
Query: 105 RKIDNLKSSSKTQ--------WRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIG 260
R D+L +S++++ W+PLLLI+P+RLG+N++N YV A K+ F++PQ +G +G
Sbjct: 199 RPPDSLTASNQSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALG 258
Query: 261 GRPSHALWFIGQVGEDLIYLDPHEPQAVVPLKTYCQVLDDESYHCTKW-SKMPIIKVDPS 437
G+P++A +FIG +G++LI+LDPH Q V + V +D+++HC + +M I+ +DPS
Sbjct: 259 GKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTV-NDQTFHCLQSPQRMNILNLDPS 317
Query: 438 LAIGFVCTSEDSFITLCEQLKRKILIPAGNYPMFEIHQKRPYYFPPLY---NCNISVNGT 608
+A+GF C E F C ++++IL N MFE+ QK P ++PP ++ G
Sbjct: 318 VALGFFCKEEKDFDNWCSLVQKEIL--KENLRMFELVQKHPSHWPPFVPPAKPEVTTTGA 375
Query: 609 D--DSAEEFE 632
+ DS E+ E
Sbjct: 376 EFIDSTEQLE 385
>sp|Q5R699|ATG4A_PONPY Cysteine protease ATG4A (Autophagy-related protein 4 homolog A)
Length = 398
Score = 133 bits (335), Expect = 6e-31
Identities = 71/188 (37%), Positives = 114/188 (60%), Gaps = 6/188 (3%)
Frame = +3
Query: 87 PPIAQARKIDNLKSSSKTQWRPLLLIIPVRLGLNELNECYVTALKDVFQLPQCVGIIGGR 266
P A + S+ + W+PLLLI+P+RLG+N++N YV A K+ F++PQ +G +GG+
Sbjct: 201 PDSLTASNLSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGK 260
Query: 267 PSHALWFIGQVGEDLIYLDPHEPQAVVPLKTYCQVLDDESYHCTKW-SKMPIIKVDPSLA 443
P++A +FIG +G++LI+LDPH Q V V +D+++HC + +M I+ +DPS+A
Sbjct: 261 PNNAYYFIGFLGDELIFLDPHTTQTFVDTGENGTV-NDQTFHCLQSPQRMNILNLDPSVA 319
Query: 444 IGFVCTSEDSFITLCEQLKRKILIPAGNYPMFEIHQKRPYYFPPLY---NCNISVNGTD- 611
+GF C E F C ++++IL N MFE+ QK P ++PP ++ G +
Sbjct: 320 LGFFCKEEKDFDNWCSLVQKEIL--KENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEF 377
Query: 612 -DSAEEFE 632
DS E+ E
Sbjct: 378 IDSTEQLE 385
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 93,380,508
Number of Sequences: 369166
Number of extensions: 1863434
Number of successful extensions: 3937
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3904
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)