Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_N16
(831 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P11586|C1TC_HUMAN C-1-tetrahydrofolate synthase, cytopla... 180 4e-45
sp|Q922D8|C1TC_MOUSE C-1-tetrahydrofolate synthase, cytopla... 175 2e-43
sp|P27653|C1TC_RAT C-1-tetrahydrofolate synthase, cytoplasm... 174 3e-43
sp|Q27772|C1TC_SPOFR C-1-tetrahydrofolate synthase, cytopla... 165 1e-40
sp|O96553|C1TC_DROME C-1-tetrahydrofolate synthase, cytopla... 160 5e-39
sp|P09440|C1TM_YEAST C-1-tetrahydrofolate synthase, mitocho... 158 2e-38
sp|P07245|C1TC_YEAST C-1-tetrahydrofolate synthase, cytopla... 157 4e-38
sp|P28723|FTHS_SPIOL Formate--tetrahydrofolate ligase (Form... 152 8e-37
sp|Q9SPK5|FTHS_ARATH Formate--tetrahydrofolate ligase (Form... 147 3e-35
sp|P21164|FTHS_MOOTH Formate--tetrahydrofolate ligase (Form... 130 6e-30
>sp|P11586|C1TC_HUMAN C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase)
[Includes: Methylenetetrahydrofolate dehydrogenase ;
Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 935
Score = 180 bits (457), Expect = 4e-45
Identities = 89/179 (49%), Positives = 120/179 (67%)
Frame = +2
Query: 14 IRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIKLAHA 193
I N + +GIPVVVA+N F D E++L+ + ++E GAFDAV +HWAEGGKGA+ LA A
Sbjct: 742 IENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQA 801
Query: 194 VEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGYDRFP 373
V+ A + PS F+ LY+L++P+E+KI IA ++YGA I + + + K ++TK G+ P
Sbjct: 802 VQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAEVYTKQGFGNLP 861
Query: 374 ICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTRPCF 550
IC+AKT LSLS N + KGVP F + V +PL GTMSTMPGLPTRPCF
Sbjct: 862 ICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASV-GAGFLYPLVGTMSTMPGLPTRPCF 919
>sp|Q922D8|C1TC_MOUSE C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase)
[Includes: Methylenetetrahydrofolate dehydrogenase ;
Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 935
Score = 175 bits (443), Expect = 2e-43
Identities = 87/179 (48%), Positives = 118/179 (65%)
Frame = +2
Query: 14 IRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIKLAHA 193
I N + +G+PVVVA+N F D E++LV + ++E GAFDAV +HWAEGG+GA+ LA A
Sbjct: 742 IENARMFGVPVVVAVNVFKTDTDAELDLVSRLSREHGAFDAVKCTHWAEGGQGALALAQA 801
Query: 194 VEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGYDRFP 373
V+ A + PS F+ LY+L++ +E+KI IA +YGA I + + + K ++TK G+ P
Sbjct: 802 VQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEAQNKAEIYTKQGFGNLP 861
Query: 374 ICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTRPCF 550
IC+AKT LSLS N + KGVP F + V +PL GTMSTMPGLPTRPCF
Sbjct: 862 ICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASV-GAGFLYPLVGTMSTMPGLPTRPCF 919
>sp|P27653|C1TC_RAT C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase)
[Includes: Methylenetetrahydrofolate dehydrogenase ;
Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 935
Score = 174 bits (440), Expect = 3e-43
Identities = 86/179 (48%), Positives = 119/179 (66%)
Frame = +2
Query: 14 IRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIKLAHA 193
I N + +G+PVVVA+N F D E++L+ + ++E GAFDAV +HWAEGG+GA+ LA A
Sbjct: 742 IENARMFGVPVVVAMNAFKTDTDTELDLIGRLSREHGAFDAVKCTHWAEGGQGALALAQA 801
Query: 194 VEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGYDRFP 373
V+ A + PS F+ LY+L++ +E+KI IA ++YGA I + + + K ++TK G+ P
Sbjct: 802 VQRASQAPSSFQLLYDLKLSVEDKIRIIAQKIYGADDIELLPEAQNKAEIYTKQGFGNLP 861
Query: 374 ICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTRPCF 550
IC+AKT LSLS N + KGVP F + V +PL GTMSTMPGLPTRPCF
Sbjct: 862 ICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASV-GAGFLYPLVGTMSTMPGLPTRPCF 919
>sp|Q27772|C1TC_SPOFR C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase)
[Includes: Methylenetetrahydrofolate dehydrogenase ;
Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 933
Score = 165 bits (418), Expect = 1e-40
Identities = 89/178 (50%), Positives = 112/178 (62%)
Frame = +2
Query: 11 HIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIKLAH 190
HI N K+G+PVVVAIN +D P E+ LV + A + GAF AVL HWA+GG GA++LA
Sbjct: 739 HISNGNKFGVPVVVAINKHGNDTPAELNLVKEFAVKNGAFRAVLCDHWAKGGLGALELAD 798
Query: 191 AVEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGYDRF 370
AV EAC S F FLY L++ ++EKI IA EMYGA + +V EKI FT +GYD+F
Sbjct: 799 AVIEACDSKSKFDFLYPLQLSIQEKIQIIAKEMYGAGQVEYTDEVLEKIKTFTDMGYDKF 858
Query: 371 PICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTRP 544
PIC+AKT SL+ + +KG P FT V P+ G +S MPGLPTRP
Sbjct: 859 PICMAKTSNSLTGDPAVKGAPTGFTLKINGIFASV-GAGFVVPMVGEISKMPGLPTRP 915
>sp|O96553|C1TC_DROME C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase)
[Includes: Methylenetetrahydrofolate dehydrogenase ;
Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 968
Score = 160 bits (404), Expect = 5e-39
Identities = 82/181 (45%), Positives = 115/181 (63%)
Frame = +2
Query: 2 MLHHIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIK 181
+L HI N K +G+PVVV++N D P E ELV K A E GAF AV+S+HWA+GG GA++
Sbjct: 771 LLQHIENGKAFGMPVVVSLNAHSADTPAEHELVKKAALEAGAFAAVVSTHWADGGAGAVQ 830
Query: 182 LAHAVEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGY 361
LA AV +AC+Q + F+ LY+LE+PL +K+NKIA MYGA + ++ +EK+ T G+
Sbjct: 831 LADAVIKACEQGNQFRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGF 890
Query: 362 DRFPICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTR 541
PIC++K S + +A++KG PK FT +CG ++ MPGLPTR
Sbjct: 891 GNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDVYVSA-GAGFVVAMCGEVTKMPGLPTR 949
Query: 542 P 544
P
Sbjct: 950 P 950
>sp|P09440|C1TM_YEAST C-1-tetrahydrofolate synthase, mitochondrial precursor (C1-THF
synthase) [Includes: Methylenetetrahydrofolate
dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 975
Score = 158 bits (400), Expect = 2e-38
Identities = 80/179 (44%), Positives = 113/179 (63%)
Frame = +2
Query: 2 MLHHIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIK 181
++ I N+K +GIPVVVAIN F D EIE++ K A GA AV S+HW EGGKGA++
Sbjct: 779 LVKQIENIKTFGIPVVVAINRFETDSQAEIEVIKKAALNAGASHAVTSNHWMEGGKGAVE 838
Query: 182 LAHAVEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGY 361
LAHAV +A K+P +F FLY++ +E+K+ I +MYG + I ++ + ++KI+ + K G+
Sbjct: 839 LAHAVVDATKEPKNFNFLYDVNSSIEDKLTSIVQKMYGGAKIEVSPEAQKKIDTYKKQGF 898
Query: 362 DRFPICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPT 538
PICIAKT SLS + LKGVP+ FTF R + + L + T+PGL T
Sbjct: 899 GNLPICIAKTQYSLSHDPSLKGVPRGFTFPI-RDVRASIGAGYLYALAAEIQTIPGLST 956
>sp|P07245|C1TC_YEAST C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase)
[Includes: Methylenetetrahydrofolate dehydrogenase ;
Methenyltetrahydrofolate cyclohydrolase ;
Formyltetrahydrofolate synthetase ]
Length = 946
Score = 157 bits (396), Expect = 4e-38
Identities = 84/179 (46%), Positives = 111/179 (62%)
Frame = +2
Query: 2 MLHHIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIK 181
M I N+K++G+PVVVAIN F D EI + K A E GAF+AV S+HWAEGGKGAI
Sbjct: 750 MCKQIANIKQFGVPVVVAINKFETDTEGEIAAIRKAALEAGAFEAVTSNHWAEGGKGAID 809
Query: 182 LAHAVEEACKQPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGY 361
LA AV EA QP DF FLY++ +E+K+ I +MYG ++I + + + KI+++ + G+
Sbjct: 810 LAKAVIEASNQPVDFHFLYDVNSSVEDKLTTIVQKMYGGAAIDILPEAQRKIDMYKEQGF 869
Query: 362 DRFPICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPT 538
PICIAKT SLS +A LKGVP FTF R + L + T+PGL T
Sbjct: 870 GNLPICIAKTQYSLSHDATLKGVPTGFTFPI-RDVRLSNGAGYLYALAAEIQTIPGLAT 927
>sp|P28723|FTHS_SPIOL Formate--tetrahydrofolate ligase (Formyltetrahydrofolate
synthetase) (10-formyletrahydrofolate synthetase) (FHS)
(FTHFS)
Length = 637
Score = 152 bits (385), Expect = 8e-37
Identities = 84/184 (45%), Positives = 112/184 (60%), Gaps = 1/184 (0%)
Frame = +2
Query: 2 MLHHIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIK 181
+ HI N K YG VVVAIN F D E+ V K A + GAFDAV+ +H A GGKGA+
Sbjct: 440 LARHIINTKAYGSNVVVAINMFSSDTEAELNAVKKAAMDAGAFDAVICTHHAHGGKGAVD 499
Query: 182 LAHAVEEACKQPSD-FKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLG 358
L AV++AC+ + +FLY L++ ++EKI IA + YGA+ + + ++KI +++K G
Sbjct: 500 LGIAVQKACENVTQPLRFLYPLDISIKEKIEAIA-KSYGAAGVEYSEQAEKKIEMYSKQG 558
Query: 359 YDRFPICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPT 538
+ PIC+AKT S S NA KG P F R + +PL GTMSTMPGLPT
Sbjct: 559 FSNLPICMAKTQYSFSHNAAEKGAPSGFILPI-RDVRGSIGAGFIYPLVGTMSTMPGLPT 617
Query: 539 RPCF 550
RPCF
Sbjct: 618 RPCF 621
>sp|Q9SPK5|FTHS_ARATH Formate--tetrahydrofolate ligase (Formyltetrahydrofolate
synthetase) (10-formyletrahydrofolate synthetase) (FHS)
(FTHFS)
Length = 634
Score = 147 bits (372), Expect = 3e-35
Identities = 79/181 (43%), Positives = 110/181 (60%), Gaps = 1/181 (0%)
Frame = +2
Query: 11 HIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIKLAH 190
HI N K YG+ V+VA+N F D E+ V K + + GAFDAV+ SH A GKGA+ L
Sbjct: 440 HISNTKAYGVNVIVAVNMFATDTEAELNAVRKFSMDAGAFDAVVCSHHAHSGKGAVDLGI 499
Query: 191 AVEEACKQ-PSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGYDR 367
AVE+AC+ +FLY L++ +++KI IA + YGAS + + +++I ++T+ G+
Sbjct: 500 AVEKACQNITQPLRFLYPLDIGIKDKIEAIA-KSYGASGVEYSDQAEKQIEMYTQQGFSN 558
Query: 368 FPICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTRPC 547
PIC++KT S S +A KG P F R + +PL GTMSTMPGLPTRPC
Sbjct: 559 LPICMSKTQYSFSHDASKKGAPSGFVLPI-RDVRGSIGAGFIYPLVGTMSTMPGLPTRPC 617
Query: 548 F 550
F
Sbjct: 618 F 618
>sp|P21164|FTHS_MOOTH Formate--tetrahydrofolate ligase (Formyltetrahydrofolate
synthetase) (FHS) (FTHFS)
Length = 559
Score = 130 bits (326), Expect = 6e-30
Identities = 71/179 (39%), Positives = 105/179 (58%), Gaps = 1/179 (0%)
Frame = +2
Query: 11 HIRNVKKYGIPVVVAINNFVHDHPDEIELVCKKAKEGGAFDAVLSSHWAEGGKGAIKLAH 190
HI N+ K+G+P VVAIN F D E+ L+ + + GA + LS WA+GG+G ++LA
Sbjct: 366 HIENIGKFGVPAVVAINAFPTDTEAELNLLYELCAKAGA-EVALSEVWAKGGEGGLELAR 424
Query: 191 AVEEACK-QPSDFKFLYNLEMPLEEKINKIAMEMYGASSISMAADVKEKINLFTKLGYDR 367
V + + +PS+F LYNL++ +++KI KIA E+YGA ++ A+ + I + LGY
Sbjct: 425 KVLQTLESRPSNFHVLYNLDLSIKDKIAKIATEIYGADGVNYTAEADKAIQRYESLGYGN 484
Query: 368 FPICIAKTPLSLSDNAQLKGVPKNFTFAY*RYK*QVLELDLFFPLCGTMSTMPGLPTRP 544
P+ +AKT S SD+ G P+NFT + L P+ G + TMPGLP RP
Sbjct: 485 LPVVMAKTQYSFSDDMTKLGRPRNFTITVREVRLSA-GGRLIVPITGAIMTMPGLPKRP 542
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,793,163
Number of Sequences: 369166
Number of extensions: 1623840
Number of successful extensions: 4267
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4233
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8052550455
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)