Planarian EST Database


Dr_sW_001_N04

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_001_N04
         (468 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P39526|YJU7_YEAST  Hypothetical 229.9 kDa protein in NUC1...    33   0.32 
sp|Q99170|GRP78_YARLI  78 kDa glucose-regulated protein homo...    32   0.55 
sp|Q6BZH1|GRP78_DEBHA  78 kDa glucose-regulated protein homo...    31   1.2  
sp|Q81GP2|OAT_BACCR  Ornithine aminotransferase (Ornithine--...    31   1.2  
sp|Q9HG01|GRP78_PICAN  78 kDa glucose-regulated protein homo...    31   1.2  
sp|P83616|GRP78_ASPNG  78 kDa glucose-regulated protein homo...    30   2.1  
sp|P36604|GRP78_SCHPO  78 kDa glucose-regulated protein homo...    30   2.7  
sp|Q81TV3|OAT_BACAN  Ornithine aminotransferase (Ornithine--...    29   4.6  
sp|P22010|GRP78_KLULA  78 kDa glucose-regulated protein homo...    29   4.6  
sp|P57528|EX5C_BUCAI  Exodeoxyribonuclease V gamma chain           29   4.6  
>sp|P39526|YJU7_YEAST Hypothetical 229.9 kDa protein in NUC1-NCE1 intergenic region
          Length = 2014

 Score = 33.1 bits (74), Expect = 0.32
 Identities = 17/63 (26%), Positives = 34/63 (53%)
 Frame = +3

Query: 255 FDFISYQLNSTDLTDSKCANFVWIDENNKFFDKHISFSGMLRNTKFENLNVDVFKKFLQY 434
           F+ I + +N   L D+   +      N+K+ D  +S SG+  + +  N +++   ++L+Y
Sbjct: 338 FESIIHLINLNLLADNTFLS------NSKYLDIVLSLSGVFSSYEVNNRSMNTLSRYLRY 391

Query: 435 FQH 443
           FQH
Sbjct: 392 FQH 394
>sp|Q99170|GRP78_YARLI 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
          Length = 670

 Score = 32.3 bits (72), Expect = 0.55
 Identities = 18/41 (43%), Positives = 23/41 (56%)
 Frame = +3

Query: 330 ENNKFFDKHISFSGMLRNTKFENLNVDVFKKFLQYFQHGLK 452
           E   FFD    FS  L   KFE LN+D+FK+ L+  +  LK
Sbjct: 315 EIESFFDGE-DFSETLTRAKFEELNIDLFKRTLKPVEQVLK 354
>sp|Q6BZH1|GRP78_DEBHA 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BIP)
          Length = 683

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 16/32 (50%), Positives = 19/32 (59%)
 Frame = +3

Query: 357 ISFSGMLRNTKFENLNVDVFKKFLQYFQHGLK 452
           I FS  L   KFE LN++ FKK L+  Q  LK
Sbjct: 335 IDFSETLSRAKFEELNIESFKKTLKPVQQVLK 366
>sp|Q81GP2|OAT_BACCR Ornithine aminotransferase (Ornithine--oxo-acid aminotransferase)
          Length = 396

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
 Frame = -3

Query: 361 EICLSKNLLF--SSIHTKLAHFESVRSVEFN**LIKSNCTPSKQIACKQIG*GNFPLSTI 188
           E+C  +N+LF    I T L+    V + +++      N TP   I  K +G G FP+S +
Sbjct: 214 EVCKKENVLFVADEIQTGLSRTGKVFACDWD------NVTPDMYILGKALGGGVFPISCV 267

Query: 187 SSPKSLRAV 161
           ++ + +  V
Sbjct: 268 AANRDILGV 276
>sp|Q9HG01|GRP78_PICAN 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
          Length = 665

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 16/31 (51%), Positives = 19/31 (61%)
 Frame = +3

Query: 357 ISFSGMLRNTKFENLNVDVFKKFLQYFQHGL 449
           I FS  L   KFE LN+D+FKK L+  Q  L
Sbjct: 326 IDFSESLSRAKFEELNMDLFKKTLKPVQQVL 356
>sp|P83616|GRP78_ASPNG 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
 sp|P83617|GRP78_ASPKA 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
 sp|P59769|GRP78_ASPAW 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
          Length = 672

 Score = 30.4 bits (67), Expect = 2.1
 Identities = 15/30 (50%), Positives = 19/30 (63%)
 Frame = +3

Query: 363 FSGMLRNTKFENLNVDVFKKFLQYFQHGLK 452
           FS  L   KFE LN+D+FKK L+  +  LK
Sbjct: 337 FSETLTRAKFEELNMDLFKKTLKPVEQVLK 366
>sp|P36604|GRP78_SCHPO 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
          Length = 663

 Score = 30.0 bits (66), Expect = 2.7
 Identities = 17/41 (41%), Positives = 23/41 (56%)
 Frame = +3

Query: 330 ENNKFFDKHISFSGMLRNTKFENLNVDVFKKFLQYFQHGLK 452
           E   FF+    FS  L   KFE +N+D+FKK L+  +  LK
Sbjct: 313 EIESFFNGQ-DFSETLSRAKFEEINMDLFKKTLKPVEQVLK 352
>sp|Q81TV3|OAT_BACAN Ornithine aminotransferase (Ornithine--oxo-acid aminotransferase)
          Length = 396

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
 Frame = -3

Query: 361 EICLSKNLLF--SSIHTKLAHFESVRSVEFN**LIKSNCTPSKQIACKQIG*GNFPLSTI 188
           E+C  +N+LF    I T L     V + +++      N TP   I  K +G G FP+S  
Sbjct: 214 EVCKKENVLFVADEIQTGLGRTGKVFACDWD------NVTPDMYILGKALGGGVFPISCA 267

Query: 187 SSPKSLRAV 161
           ++ + +  V
Sbjct: 268 AANRDILGV 276
>sp|P22010|GRP78_KLULA 78 kDa glucose-regulated protein homolog precursor (GRP 78)
           (Immunoglobulin heavy chain binding protein homolog)
           (BiP)
          Length = 679

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 18/41 (43%), Positives = 24/41 (58%)
 Frame = +3

Query: 330 ENNKFFDKHISFSGMLRNTKFENLNVDVFKKFLQYFQHGLK 452
           E + FF+  I FS  L   KFE LN+ +FKK L+  +  LK
Sbjct: 329 EIDSFFNG-IDFSETLTRAKFEELNLALFKKTLKPVEKVLK 368
>sp|P57528|EX5C_BUCAI Exodeoxyribonuclease V gamma chain
          Length = 1070

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = +3

Query: 330 ENNKFFDKHISFSGMLRNTKFENLNVDVFKKFLQY 434
           +N KFF+       M++  KF  L   +FKK++ Y
Sbjct: 100 DNKKFFENCSKKKDMIKKFKFSFLMASIFKKYILY 134
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,951,441
Number of Sequences: 369166
Number of extensions: 920014
Number of successful extensions: 2044
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2005
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2044
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2683624230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)