Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_N02
(858 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q6AYM9|CLN8_RAT Protein CLN8 52 2e-06
sp|Q9QUK3|CLN8_MOUSE Protein CLN8 48 4e-05
sp|Q9UBY8|CLN8_HUMAN Protein CLN8 45 2e-04
sp|Q5JZQ7|CLN8_CANFA Protein CLN8 39 0.014
sp|P47153|YJ86_YEAST Hypothetical 32.0 kDa protein in NNF1-... 36 0.12
sp|Q8W4Y5|ASCL_LYCES ASC1-like protein (Alternaria stem can... 33 1.3
sp|Q9R0B6|LAMC3_MOUSE Laminin gamma-3 chain precursor (Lami... 31 3.9
sp|Q96G23|LASS2_HUMAN LAG1 longevity assurance homolog 2 (T... 31 5.0
sp|O59735|YHXF_SCHPO Hypothetical protein C3E17.15c in chro... 31 5.0
sp|Q99PE7|ABCG5_RAT ATP-binding cassette sub-family G membe... 30 8.6
>sp|Q6AYM9|CLN8_RAT Protein CLN8
Length = 288
Score = 52.4 bits (124), Expect = 2e-06
Identities = 54/241 (22%), Positives = 106/241 (43%), Gaps = 13/241 (5%)
Frame = +3
Query: 30 YKWILGSFFCCMTVHHLISPKLFGTFCESYKKLKKPKQMEWDSRIVSTIHALIVSLMSLY 209
+ + LG F C H +S L T Y+ L +++ W+ + + + L+
Sbjct: 32 FVFYLGVFVVC----HQLSSSLNAT----YRSLLAKEKVFWNLAATRAVFGIQSTAAGLW 83
Query: 210 ALIFETQIWSNRISYASRIGSRALSITVGYFLCDMISMPM---YWRGRSLIIFLIHHAFS 380
AL+ + ++SN+ ++ G+F + ++ + ++R L + ++HH F+
Sbjct: 84 ALLGDPVLYSNKALGQQNWCWFHITTATGFFFFENAAVHLSNLFFRTFDLFL-VVHHLFA 142
Query: 381 AGAFL-YAINIQSCIFFCIIRLTSELSTPFTNQRWIFLTSGLSHGHKKVVYSTLVFAVLF 557
FL A+N+++ + + L E+STPFT W+ L +G SH LF
Sbjct: 143 FLGFLGSAVNLRAGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSHS-------------LF 189
Query: 558 SIVRNWIIVPYW----IMVYFDYGT-----EAHLQSKSINHFLDYVWLGCAGVLDILNIY 710
V W+++ + I+ Y + +A S + H +++ G A + I+N Y
Sbjct: 190 WKVNQWLMIHMFHCRMILTYHMWWVCFQHWDALASSLYLPHLALFLF-GLALLTVIINPY 248
Query: 711 W 713
W
Sbjct: 249 W 249
>sp|Q9QUK3|CLN8_MOUSE Protein CLN8
Length = 288
Score = 47.8 bits (112), Expect = 4e-05
Identities = 50/254 (19%), Positives = 113/254 (44%), Gaps = 9/254 (3%)
Frame = +3
Query: 30 YKWILGSFFCCMTVHHLISPKLFGTFCESYKKLKKPKQMEWDSRIVSTIHALIVSLMSLY 209
+ + LG F C H +S L T Y+ L +++ W+ + + + L+
Sbjct: 32 FVFYLGVFVVC----HQLSSSLNAT----YRSLAAKEKVFWNLAATRAVFGVQSTTAGLW 83
Query: 210 ALIFETQIWSNRISYASRIGSRALSITVGYFLCDMISMPM---YWRGRSLIIFLIHHAFS 380
AL+ + +++++ ++ G+F + +++ + ++R L + ++HH F+
Sbjct: 84 ALLGDPVLYADKALGQQNWCWFHITTATGFFFFENVAVHLSNLFFRTFDLFL-VVHHLFA 142
Query: 381 AGAFL-YAINIQSCIFFCIIRLTSELSTPFTNQRWIFLTSGLS-----HGHKKVVYSTLV 542
FL AIN+++ + + L E+STPFT W+ L +G S ++ ++
Sbjct: 143 FLGFLGSAINLRAGHYLAMTTLLLEMSTPFTCISWMLLKAGWSDSLFWKANQWLMIHMFH 202
Query: 543 FAVLFSIVRNWIIVPYWIMVYFDYGTEAHLQSKSINHFLDYVWLGCAGVLDILNIYWGPG 722
++ + W+ +W +A S + H+ +++ G A + ++N YW
Sbjct: 203 CRMILTYHMWWVCFCHW---------DALTSSLHLPHWALFLF-GLALLTAVINPYW--- 249
Query: 723 VYRSGRRAFQAVHP 764
+ ++ Q +HP
Sbjct: 250 ---THKKTQQLLHP 260
>sp|Q9UBY8|CLN8_HUMAN Protein CLN8
Length = 286
Score = 45.4 bits (106), Expect = 2e-04
Identities = 53/243 (21%), Positives = 100/243 (41%), Gaps = 15/243 (6%)
Frame = +3
Query: 30 YKWILGSFFCCMTVHHLISPKLFGTFCESYKKLKKPKQMEWDSRIVSTIHALIVSLMSLY 209
+ + LG F C H +S L T Y+ L +++ WD + + + L+
Sbjct: 32 FVFYLGVFVVC----HQLSSSLNAT----YRSLVAREKVFWDLAATRAVFGVQSTAAGLW 83
Query: 210 ALIFETQIWSNRISYASRIGSRALSITVGYFLCDMISMPMY-WRGRSLIIFL-IHHAFSA 383
AL+ + + +++ ++ G+F + +++ + R+ +FL IHH F+
Sbjct: 84 ALLGDPVLHADKARGQQNWCWFHITTATGFFCFENVAVHLSNLIFRTFDLFLVIHHLFAF 143
Query: 384 GAFLYA-INIQSCIFFCIIRLTSELSTPFTNQRWIFLTSGLSHGHKKVVYSTLVFAVLFS 560
FL +N+Q + + L E+STPFT W+ L +G S LF
Sbjct: 144 LGFLGCLVNLQVGHYLAMTTLLLEMSTPFTCVSWMLLKAGWSES-------------LFW 190
Query: 561 IVRNWIIV------------PYWIMVYFDYGTEAHLQSKSINHFLDYVWLGCAGVLDILN 704
+ W+++ +W+ + G + L + FL +G A + I+N
Sbjct: 191 KLNQWLMIHMFHCRMVLTYHMWWVCFWHWDGLVSSLYLPHLTLFL----VGLALLTLIIN 246
Query: 705 IYW 713
YW
Sbjct: 247 PYW 249
>sp|Q5JZQ7|CLN8_CANFA Protein CLN8
Length = 288
Score = 39.3 bits (90), Expect = 0.014
Identities = 37/162 (22%), Positives = 74/162 (45%), Gaps = 3/162 (1%)
Frame = +3
Query: 30 YKWILGSFFCCMTVHHLISPKLFGTFCESYKKLKKPKQMEWDSRIVSTIHALIVSLMSLY 209
+ + LG F C H +S L T Y+ L +++ W+ + + + L+
Sbjct: 32 FVFYLGVFVVC----HQLSSSLNAT----YRSLVAREKVFWNLAATRAVFGVQSTAAGLW 83
Query: 210 ALIFETQIWSNRISYASRIGSRALSITVGYFLCDMISMPMY-WRGRSLIIFL-IHHAFSA 383
AL+ + + +++ ++ G+F + +++ + R+ +FL IHH F+
Sbjct: 84 ALLVDPVLHADKARGQQNWCWFHIATATGFFFFENVAVHLSNVLFRTFDLFLAIHHLFAF 143
Query: 384 GAFLYAI-NIQSCIFFCIIRLTSELSTPFTNQRWIFLTSGLS 506
FL ++ N+ + + + L E STPFT W+ L +G S
Sbjct: 144 LGFLGSVVNLGAGHYLAMSTLLLEASTPFTCISWMLLKAGWS 185
>sp|P47153|YJ86_YEAST Hypothetical 32.0 kDa protein in NNF1-STE24 intergenic region
Length = 279
Score = 36.2 bits (82), Expect = 0.12
Identities = 44/186 (23%), Positives = 76/186 (40%), Gaps = 7/186 (3%)
Frame = +3
Query: 39 ILGSFFCCMTVHHLISPKLFGT-FCESYKKLKKPK-QMEWDSRIVSTIHALIVSLMSLYA 212
I+ SF +++P L F + Y ++ P +++++ VS I A++ + + L
Sbjct: 36 IIFSFVFYQLAFSVVAPFLNKVVFRKHYTTIRDPLLKIDFNVHTVSMIQAVVSNTVLLPT 95
Query: 213 LIFETQIWSNRISYASRIGSRALSITVGYFLCDMISMPMYWRGRSLIIFLIHHAFSAGAF 392
L T + N ++Y S S++ GYF+ D+ Y++ L F H S
Sbjct: 96 LT--TPMHYNVVTYTDSYSSMVSSLSAGYFIWDLTMCVRYFKLYGLE-FTGHAIGSVYVM 152
Query: 393 LYAINIQSCIFFCIIRLTSELSTPFTNQRWIFLTSGLSHGHK-----KVVYSTLVFAVLF 557
L ++ C + L E STPF N W + + VV L+ V F
Sbjct: 153 LLSLR-PFCQPWIGRFLIYEASTPFVNINWFIMQCNAKSKNSIPLWFNVVNGLLLMTVFF 211
Query: 558 SIVRNW 575
+ W
Sbjct: 212 VVRICW 217
>sp|Q8W4Y5|ASCL_LYCES ASC1-like protein (Alternaria stem canker resistance-like protein)
Length = 303
Score = 32.7 bits (73), Expect = 1.3
Identities = 41/184 (22%), Positives = 76/184 (41%), Gaps = 8/184 (4%)
Frame = +3
Query: 228 QIWSNRISYASRIGSRALSITVGYFLCDMISMPMYWRGR--SLIIFLIHHAFSAGAFLYA 401
Q+W +++ Y S++ +AL + G F I ++W R + + HH +A + +
Sbjct: 114 QVWPDQM-YKSKL--KALYMYTGGFYTYSIFALIFWETRRSDFGVSMSHHVATAILIVLS 170
Query: 402 INIQSCIFFCIIRLTSELSTPFTNQRWIFLTSG-LSHGHKKVVYSTLVFAVLFSIVRNWI 578
NI+ ++ + S IFL G +S ++ + L I+ I
Sbjct: 171 YNIRFARVGSVVLAIHDASD-------IFLEIGKMSKYSGAEALASFRYLCLSWIILRLI 223
Query: 579 IVPYWIMVYFDYGTEAHLQSKSINHFLD-----YVWLGCAGVLDILNIYWGPGVYRSGRR 743
P+W++ Y L + H +D Y++ L +L+IYW +YR +
Sbjct: 224 YYPFWVLWSTSYEVLQTLDKEK--HKVDGPIYYYIFNSLLFCLLVLHIYWWVLIYRMLVK 281
Query: 744 AFQA 755
QA
Sbjct: 282 QIQA 285
>sp|Q9R0B6|LAMC3_MOUSE Laminin gamma-3 chain precursor (Laminin 12 gamma 3 subunit)
Length = 1581
Score = 31.2 bits (69), Expect = 3.9
Identities = 19/35 (54%), Positives = 22/35 (62%)
Frame = -1
Query: 786 LSFLLKKLDGRLGMHDAQSDILQDPNRYLECLRLQ 682
LS LL KL G LG+H A + L + R LE LRLQ
Sbjct: 1493 LSELLVKL-GSLGVHQAPAQTLNETQRALESLRLQ 1526
>sp|Q96G23|LASS2_HUMAN LAG1 longevity assurance homolog 2 (Tumor metastasis-suppressor
gene 1 protein) (SP260)
Length = 380
Score = 30.8 bits (68), Expect = 5.0
Identities = 20/69 (28%), Positives = 35/69 (50%)
Frame = +3
Query: 537 LVFAVLFSIVRNWIIVPYWIMVYFDYGTEAHLQSKSINHFLDYVWLGCAGVLDILNIYWG 716
+VFA++F I R +I+P+WI+ + T + F Y + GVL +L+I+W
Sbjct: 266 IVFAIVFIITR-LVILPFWIL----HCTLVYPLELYPAFFGYYFFNSMMGVLQLLHIFWA 320
Query: 717 PGVYRSGRR 743
+ R +
Sbjct: 321 YLILRMAHK 329
>sp|O59735|YHXF_SCHPO Hypothetical protein C3E17.15c in chromosome II
Length = 384
Score = 30.8 bits (68), Expect = 5.0
Identities = 40/177 (22%), Positives = 71/177 (40%), Gaps = 24/177 (13%)
Frame = +3
Query: 291 VGYFLCDMISMPMYWRGRSLIIFL-------------IHHAFSAGAFLYAINIQSCIFFC 431
VG F + YW ++L++ L +HH + L I + F
Sbjct: 190 VGSFKAFYLIEAAYWIQQALVLILQLEKPRKDFKELVVHHIIT----LLLIGLSYYFHFT 245
Query: 432 IIRLTSELSTPFTNQRWIFLTSGLSHGHKKVVYSTLVFAVLFSIVRNWIIVPYWI---MV 602
I L + T T+ W+ L+ L++ + +VY V V WI + +++ ++
Sbjct: 246 WIGLAVFI-TMDTSDIWLALSKCLNYVNTVIVYPIFVIFVFV-----WIYMRHYLNFKIM 299
Query: 603 YFDYGTEAHLQSKSINHFLDY--------VWLGCAGVLDILNIYWGPGVYRSGRRAF 749
+ +GT + S ++ + V L L ++NIYW + R G RAF
Sbjct: 300 WAVWGTMRTINSFDLDWAAEQYKCWISRDVTLILLTALQLVNIYWLILILRIGYRAF 356
>sp|Q99PE7|ABCG5_RAT ATP-binding cassette sub-family G member 5 (Sterolin-1)
Length = 652
Score = 30.0 bits (66), Expect = 8.6
Identities = 30/108 (27%), Positives = 47/108 (43%), Gaps = 1/108 (0%)
Frame = +3
Query: 384 GAFLYAINIQSCIFFCIIRLTSEL-STPFTNQRWIFLTSGLSHGHKKVVYSTLVFAVLFS 560
GA Y + + F ++R S+ S Q+W L + + H + ++V V+FS
Sbjct: 429 GATPYTGMLNAVNLFPMLRAVSDQESQDGLYQKWQMLLAYVLHA----LPFSIVATVIFS 484
Query: 561 IVRNWIIVPYWIMVYFDYGTEAHLQSKSINHFLDYVWLGCAGVLDILN 704
V W + Y + F Y + A L I FL V LG +I+N
Sbjct: 485 SVCYWTLGLYPEVARFGYFSAALLAPHLIGEFLTLVLLGMVQNPNIVN 532
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,633,410
Number of Sequences: 369166
Number of extensions: 1826795
Number of successful extensions: 4326
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4323
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)