Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_M21
(853 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8CGC7|SYEP_MOUSE Bifunctional aminoacyl-tRNA synthetase... 235 1e-61
sp|P07814|SYEP_HUMAN Bifunctional aminoacyl-tRNA synthetase... 233 5e-61
sp|P28668|SYEP_DROME Bifunctional aminoacyl-tRNA synthetase... 211 3e-54
sp|O13775|SYEC_SCHPO Probable glutamyl-tRNA synthetase, cyt... 119 8e-27
sp|P46655|SYEC_YEAST Glutamyl-tRNA synthetase, cytoplasmic ... 104 3e-22
sp|Q8Z8F8|SYQ_SALTI Glutaminyl-tRNA synthetase (Glutamine--... 49 2e-05
sp|Q8ZQX5|SYQ_SALTY Glutaminyl-tRNA synthetase (Glutamine--... 48 3e-05
sp|Q8X9H8|SYQ_ECO57 Glutaminyl-tRNA synthetase (Glutamine--... 45 3e-04
sp|Q87DU6|SYQ_XYLFT Glutaminyl-tRNA synthetase (Glutamine--... 45 3e-04
sp|Q83LY4|SYQ_SHIFL Glutaminyl-tRNA synthetase (Glutamine--... 44 7e-04
>sp|Q8CGC7|SYEP_MOUSE Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (Glutamate--tRNA ligase); Prolyl-tRNA
synthetase (Proline--tRNA ligase)]
Length = 1512
Score = 235 bits (600), Expect = 1e-61
Identities = 120/247 (48%), Positives = 168/247 (68%)
Frame = +1
Query: 73 KKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAIGKKAIWFTPSVYVE 252
KK DP+ PRY AL +K +V V ++D ++ K+ V +HPKN +G K +W++P V++E
Sbjct: 497 KKVIDPVAPRYVALL--KKEVVPVNVLDAQEEMKE-VARHPKNPDVGLKPVWYSPKVFIE 553
Query: 253 MNDAVDFVEGTNVTFINWGNILITKIHRNSENNITFIEGKLNLSDRDFKKTQKITWMPLG 432
DA F EG VTFINWGNI ITKIH+N++ IT ++ KLNL ++D+KKT KITW+
Sbjct: 554 GADAETFSEGEMVTFINWGNINITKIHKNADGKITSLDAKLNLENKDYKKTTKITWLA-- 611
Query: 433 INDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEECLIGDIELKNLKKGEI 612
+ + IP + Y+++I+K VLGKD+DFK ++NK+SK EE ++GD LK+LKKG+I
Sbjct: 612 --ESTHALPIPAVCVTYEHLITKPVLGKDEDFKQYINKDSKHEELMLGDPCLKDLKKGDI 669
Query: 613 IQLQRRGFFICDEEYQEKCKVTGNELPLVLINIPEGNTKERPTVGMKQKSVPNSSKPSSG 792
IQLQRRGFFICD+ Y+ + E P +LI IP+G+TKE PT G K+K+ SK +
Sbjct: 670 IQLQRRGFFICDQPYEPVSPYSCREAPCILIYIPDGHTKEMPTSGSKEKTKVEISKKETS 729
Query: 793 DHIDKER 813
KER
Sbjct: 730 S-APKER 735
Score = 41.6 bits (96), Expect = 0.003
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +3
Query: 3 FVLAQGFGRANVVMEWDKLWASNKKVI 83
F+ AQG R+ V MEWDK+WA NKKVI
Sbjct: 474 FIAAQGSSRSVVNMEWDKIWAFNKKVI 500
>sp|P07814|SYEP_HUMAN Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (Glutamate--tRNA ligase); Prolyl-tRNA
synthetase (Proline--tRNA ligase)]
Length = 1440
Score = 233 bits (594), Expect = 5e-61
Identities = 119/260 (45%), Positives = 172/260 (66%)
Frame = +1
Query: 73 KKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAIGKKAIWFTPSVYVE 252
KK DP+ PRY AL +K ++ V + + ++ K+ V KHPKN +G K +W++P V++E
Sbjct: 425 KKVIDPVAPRYVALL--KKEVIPVNVPEAQEEMKE-VAKHPKNPEVGLKPVWYSPKVFIE 481
Query: 253 MNDAVDFVEGTNVTFINWGNILITKIHRNSENNITFIEGKLNLSDRDFKKTQKITWMPLG 432
DA F EG VTFINWGN+ ITKIH+N++ I ++ K NL ++D+KKT K+TW+
Sbjct: 482 GADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKFNLENKDYKKTTKVTWLA-- 539
Query: 433 INDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEECLIGDIELKNLKKGEI 612
+ + IP+ + Y+++I+K VLGKD+DFK ++NKNSK EE ++GD LK+LKKG+I
Sbjct: 540 --ETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDI 597
Query: 613 IQLQRRGFFICDEEYQEKCKVTGNELPLVLINIPEGNTKERPTVGMKQKSVPNSSKPSSG 792
IQLQRRGFFICD+ Y+ + E P VLI IP+G+TKE PT G K+K+ ++K +
Sbjct: 598 IQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETS 657
Query: 793 DHIDKERSSNLKQTNTTESK 852
KER + N T S+
Sbjct: 658 APF-KERPTPSLNNNCTTSE 676
Score = 41.6 bits (96), Expect = 0.003
Identities = 18/27 (66%), Positives = 21/27 (77%)
Frame = +3
Query: 3 FVLAQGFGRANVVMEWDKLWASNKKVI 83
F+ AQG R+ V MEWDK+WA NKKVI
Sbjct: 402 FIAAQGSSRSVVNMEWDKIWAFNKKVI 428
>sp|P28668|SYEP_DROME Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA
synthetase (Glutamate--tRNA ligase); Prolyl-tRNA
synthetase (Proline--tRNA ligase)]
Length = 1714
Score = 211 bits (536), Expect = 3e-54
Identities = 113/258 (43%), Positives = 160/258 (62%), Gaps = 5/258 (1%)
Frame = +1
Query: 73 KKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAIGKKAIWFTPSVYVE 252
KK DPI PRYTAL ++ V+V + K +E+ V HPK++++GKK + P +Y++
Sbjct: 503 KKVIDPIAPRYTALEKEKR--VIVNVAGAK-VERIQVSVHPKDESLGKKTVLLGPRIYID 559
Query: 253 MNDAVDFVEGTNVTFINWGNILITKIHRNSENNITFIEGKLNLSDRDFKKTQKITWMPLG 432
DA EG N TFINWGNILI K+++++ NIT ++ LNL ++DFKKT K+TW L
Sbjct: 560 YVDAEALKEGENATFINWGNILIRKVNKDASGNITSVDAALNLENKDFKKTLKLTW--LA 617
Query: 433 INDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEECLIGDIELKNLKKGEI 612
+ D+ ++ P +++DNIISK VLGKD+DFK F+ ++ E ++GD ELK KKG+I
Sbjct: 618 VEDDPSAYP-PTFCVYFDNIISKAVLGKDEDFKQFIGHKTRDEVPMLGDPELKKCKKGDI 676
Query: 613 IQLQRRGFFICDEEYQEKCKVTGNELPLVLINIPEGNTKERPTVGMK-----QKSVPNSS 777
IQLQRRGFF D Y T P+VL +IP+G+TK+ PT G+K K+ +S
Sbjct: 677 IQLQRRGFFKVDVAYAPPSGYTNVPSPIVLFSIPDGHTKDVPTSGLKVNAPDAKATKKAS 736
Query: 778 KPSSGDHIDKERSSNLKQ 831
P S E S + Q
Sbjct: 737 SPVSSSGQASELDSQISQ 754
Score = 40.4 bits (93), Expect = 0.006
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +3
Query: 3 FVLAQGFGRANVVMEWDKLWASNKKVI 83
F++AQG ++ V M WDK+WA NKKVI
Sbjct: 480 FIIAQGSSKSVVFMNWDKIWAFNKKVI 506
>sp|O13775|SYEC_SCHPO Probable glutamyl-tRNA synthetase, cytoplasmic (Glutamate--tRNA
ligase) (GluRS)
Length = 716
Score = 119 bits (299), Expect = 8e-27
Identities = 77/236 (32%), Positives = 131/236 (55%), Gaps = 2/236 (0%)
Frame = +1
Query: 34 TL*WSGINYGLLIKKSFDPIVPRYTAL-SGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAI 210
TL W+ ++ KK DP+ PR+TA+ SG +V E + +H KN +
Sbjct: 495 TLDWT--SFWATNKKIIDPVAPRHTAVESGDVVKATIVNGPAAPYAEDR--PRHKKNPEL 550
Query: 211 GKKAIWFTPSVYVEMNDAVDFVEGTNVTFINWGNILITKIHRNSENNITFIEGKLNLSDR 390
G K F + +E DA F + VT ++WGN + +I+R++ +T ++ +L+L D
Sbjct: 551 GNKKSIFANEILIEQADAQSFKQDEEVTLMDWGNAYVREINRDASGKVTSLKLELHL-DG 609
Query: 391 DFKKTQ-KITWMPLGINDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEEC 567
DFKKT+ K+TW+ + E K+ P+ + +D +I+K+ L + +++K+FL ++
Sbjct: 610 DFKKTEKKVTWL---ADTEDKT---PVDLVDFDYLITKDKLEEGENYKDFLTPQTEFHSP 663
Query: 568 LIGDIELKNLKKGEIIQLQRRGFFICDEEYQEKCKVTGNELPLVLINIPEGNTKER 735
+ D+ +KNLKKG+IIQ++R+G++I D V + VL NIP+G T R
Sbjct: 664 VFADVGIKNLKKGDIIQVERKGYYIVD--------VPFDGTQAVLFNIPDGKTVNR 711
Score = 30.0 bits (66), Expect = 8.5
Identities = 9/27 (33%), Positives = 19/27 (70%)
Frame = +3
Query: 3 FVLAQGFGRANVVMEWDKLWASNKKVI 83
++++QG + + ++W WA+NKK+I
Sbjct: 483 YIVSQGPSKNILTLDWTSFWATNKKII 509
>sp|P46655|SYEC_YEAST Glutamyl-tRNA synthetase, cytoplasmic (Glutamate--tRNA ligase)
(GluRS) (P85)
Length = 724
Score = 104 bits (260), Expect = 3e-22
Identities = 68/219 (31%), Positives = 122/219 (55%), Gaps = 1/219 (0%)
Frame = +1
Query: 73 KKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAIGKKAIWFTPSVYVE 252
KK DPI PR+TA+ K + L ++ + + KH KN A+G+K + + + V+
Sbjct: 518 KKVIDPIAPRHTAIVNPVK-IHLEGSEAPQEPKIEMKPKHKKNPAVGEKKVIYYKDIVVD 576
Query: 253 MNDAVDFVEGTNVTFINWGNILITKIHRNSENNITFIEGKLNLSDRDFKKTQ-KITWMPL 429
+DA VT ++WGN++ITK +N + ++ KLNL + DFKKT+ K+TW+
Sbjct: 577 KDDADVINVDEEVTLMDWGNVIITK--KNDDGSMV---AKLNL-EGDFKKTKHKLTWLA- 629
Query: 430 GINDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEECLIGDIELKNLKKGE 609
+ K +P+ + +D++I+K+ L +D+ F++FL ++ I D+ +K++K G+
Sbjct: 630 ----DTKDV-VPVDLVDFDHLITKDRLEEDESFEDFLTPQTEFHTDAIADLNVKDMKIGD 684
Query: 610 IIQLQRRGFFICDEEYQEKCKVTGNELPLVLINIPEGNT 726
IIQ +R+G++ D + + P V IP+G +
Sbjct: 685 IIQFERKGYYRLD-------ALPKDGKPYVFFTIPDGKS 716
Score = 34.7 bits (78), Expect = 0.34
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +3
Query: 3 FVLAQGFGRANVVMEWDKLWASNKKVI 83
FVL+QG R + +EW+ +WA NKKVI
Sbjct: 495 FVLSQGPSRNVINLEWNLIWAFNKKVI 521
>sp|Q8Z8F8|SYQ_SALTI Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Length = 555
Score = 48.9 bits (115), Expect = 2e-05
Identities = 47/219 (21%), Positives = 91/219 (41%), Gaps = 14/219 (6%)
Frame = +1
Query: 70 IKKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAIGKKAIWFTPSVYV 249
I++ + PR A+ K ++++ + + E + HP +G + + F+ +++
Sbjct: 332 IREDLNENAPRAMAVIDPVK--LVIENYPQGESEMVTMPNHPNKPEMGSREVPFSGEIWI 389
Query: 250 EMNDAVD--------FVEGTNVTFINWGNILITKIHRNSENNITFI-----EGKLNLSDR 390
+ D + V G V N I ++ +++E NIT I L+
Sbjct: 390 DRADFREEANKQYKRLVMGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPA 449
Query: 391 DFKKTQKIT-WMPLGINDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEEC 567
D +K + + W+ + + ++PI YD + S G +DF + +N S +
Sbjct: 450 DGRKVKGVIHWVSV-------AHALPIEIRLYDRLFSVPNPGAAEDFLSVINPESLVIKQ 502
Query: 568 LIGDIELKNLKKGEIIQLQRRGFFICDEEYQEKCKVTGN 684
G+ LK G+ Q +R G+F D Y K+ N
Sbjct: 503 GYGEPSLKAAVAGKAFQFEREGYFCLDSRYATADKLVFN 541
>sp|Q8ZQX5|SYQ_SALTY Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Length = 555
Score = 48.1 bits (113), Expect = 3e-05
Identities = 47/219 (21%), Positives = 90/219 (41%), Gaps = 14/219 (6%)
Frame = +1
Query: 70 IKKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFVDKHPKNDAIGKKAIWFTPSVYV 249
I++ + PR A+ K ++++ + + E + HP +G + + F+ +++
Sbjct: 332 IREDLNENAPRAMAVIDPVK--LVIENYPQGESEMVTMPNHPNKPEMGSREVPFSGEIWI 389
Query: 250 EMNDAVD--------FVEGTNVTFINWGNILITKIHRNSENNITFI-----EGKLNLSDR 390
+ D + V G V N I ++ +++E NIT I L+
Sbjct: 390 DRADFREEANKQYKRLVMGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPA 449
Query: 391 DFKKTQKIT-WMPLGINDEVKSFSIPISSIFYDNIISKNVLGKDDDFKNFLNKNSKKEEC 567
D +K + + W+ + ++PI YD + S G +DF + +N S +
Sbjct: 450 DGRKVKGVIHWVSA-------AHALPIEIRLYDRLFSVPNPGAAEDFLSVINPESLVIKQ 502
Query: 568 LIGDIELKNLKKGEIIQLQRRGFFICDEEYQEKCKVTGN 684
G+ LK G+ Q +R G+F D Y K+ N
Sbjct: 503 GYGEPSLKAAVAGKAFQFEREGYFCLDSRYATADKLVFN 541
>sp|Q8X9H8|SYQ_ECO57 Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Length = 554
Score = 44.7 bits (104), Expect = 3e-04
Identities = 37/170 (21%), Positives = 71/170 (41%), Gaps = 14/170 (8%)
Frame = +1
Query: 190 HPKNDAIGKKAIWFTPSVYVEMNDAVD--------FVEGTNVTFINWGNILITKIHRNSE 345
HP +G + + F+ ++++ +D + V G V N I ++ +++E
Sbjct: 369 HPNKPEMGSRQVPFSGEIWIDRSDFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAE 428
Query: 346 NNITFI-----EGKLNLSDRDFKKTQKIT-WMPLGINDEVKSFSIPISSIFYDNIISKNV 507
NIT I L+ D +K + + W+ + ++P+ YD + S
Sbjct: 429 GNITTIFCTYDADTLSKDPADGRKVKGVIHWVSA-------AHALPVEIRLYDRLFSVPN 481
Query: 508 LGKDDDFKNFLNKNSKKEECLIGDIELKNLKKGEIIQLQRRGFFICDEEY 657
G DDF + +N S + + LK+ G+ Q +R G+F D +
Sbjct: 482 PGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRH 531
>sp|Q87DU6|SYQ_XYLFT Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Length = 580
Score = 44.7 bits (104), Expect = 3e-04
Identities = 53/218 (24%), Positives = 95/218 (43%), Gaps = 25/218 (11%)
Frame = +1
Query: 70 IKKSFDPIVPRYTALSGSEKSLVLVKIIDKKDIEKQFV-DKHPKNDAIGKKAIWFTPSVY 246
++++ D I PR A K LVL + + D E+Q + HPK+D G + + F+ ++
Sbjct: 356 LRENLDTIAPRRMATIAPMK-LVLTNLAE--DHEEQLIFPNHPKDDTQGTRTVPFSRELW 412
Query: 247 VEMNDAVD--------FVEGTNVTFINWGNILITKIHRNSENNITFIEGKLNLSDR---- 390
+E +D + + G V G I ++ +N+E ++ + G L+ + R
Sbjct: 413 IERDDFSEAPPKGWKRLIPGGEVRLRGAGIARIDEVVKNAEGHVIALHGWLDPTSRPGME 472
Query: 391 --DFKKTQKITWM--PLGINDEVKSFSIPISSIFYDNIISKNVLGKDDD------FKNFL 540
K I W+ P + E++ YD + S + K DD +++FL
Sbjct: 473 GAHRKVKGTIHWVSAPHAVAAEIR---------LYDRLFS---IEKPDDNTDGKTYRDFL 520
Query: 541 NKNSKKEECLIGDIE--LKNLKKGEIIQLQRRGFFICD 648
N +SK+ + G IE Q +R G+F+ D
Sbjct: 521 NPDSKR--VVHGYIEPAAAQTAPEHAFQFERLGYFVTD 556
>sp|Q83LY4|SYQ_SHIFL Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Length = 554
Score = 43.5 bits (101), Expect = 7e-04
Identities = 37/170 (21%), Positives = 70/170 (41%), Gaps = 14/170 (8%)
Frame = +1
Query: 190 HPKNDAIGKKAIWFTPSVYVEMNDAVD--------FVEGTNVTFINWGNILITKIHRNSE 345
HP +G + + F+ ++++ D + V G V N I ++ +++E
Sbjct: 369 HPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAE 428
Query: 346 NNITFI-----EGKLNLSDRDFKKTQKIT-WMPLGINDEVKSFSIPISSIFYDNIISKNV 507
NIT I L+ D +K + + W+ + ++P+ YD + S
Sbjct: 429 GNITTIFCTYDADTLSKDPADGRKVKGVIHWVSA-------AHALPVEIRLYDRLFSVPN 481
Query: 508 LGKDDDFKNFLNKNSKKEECLIGDIELKNLKKGEIIQLQRRGFFICDEEY 657
G DDF + +N S + + LK+ G+ Q +R G+F D +
Sbjct: 482 PGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRH 531
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 89,358,103
Number of Sequences: 369166
Number of extensions: 1726286
Number of successful extensions: 4831
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4647
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4814
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8390082510
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)