Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_L18
(941 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P12890|AMD2_XENLA Peptidyl-glycine alpha-amidating monoo... 186 1e-46
sp|P08478|AMD1_XENLA Peptidyl-glycine alpha-amidating monoo... 185 1e-46
sp|P10731|AMD_BOVIN Peptidyl-glycine alpha-amidating monoox... 182 2e-45
sp|P97467|AMD_MOUSE Peptidyl-glycine alpha-amidating monoox... 180 5e-45
sp|P14925|AMD_RAT Peptidyl-glycine alpha-amidating monooxyg... 178 2e-44
sp|P19021|AMD_HUMAN Peptidyl-glycine alpha-amidating monoox... 177 3e-44
sp|P09172|DOPO_HUMAN Dopamine beta-hydroxylase precursor (D... 60 7e-09
sp|Q05754|DOPO_RAT Dopamine beta-hydroxylase precursor (Dop... 57 8e-08
sp|Q64237|DOPO_MOUSE Dopamine beta-hydroxylase precursor (D... 53 1e-06
sp|P15101|DOPO_BOVIN Dopamine beta-hydroxylase precursor (D... 53 1e-06
>sp|P12890|AMD2_XENLA Peptidyl-glycine alpha-amidating monooxygenase II precursor
(Peptide C-terminal alpha-amidating enzyme II) (AE-II)
Length = 875
Score = 186 bits (471), Expect = 1e-46
Identities = 94/245 (38%), Positives = 142/245 (57%), Gaps = 3/245 (1%)
Frame = +1
Query: 31 LKMPGVRPTKEDEYICHAVDLK-NETFYITGFQPKANATHAHHILIHSCIEPGSMKSSWN 207
++MPGV PT+ D Y+C + L ++ Y+ ++P AN AHH+L+ C P S W+
Sbjct: 64 IRMPGVTPTESDTYLCKSYRLPVDDEAYVVDYRPHANMDTAHHMLLFGCNVPSSTDDYWD 123
Query: 208 CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSIGKQYLVLQSHYKQVDY 387
C + C ++Y+W NA +LP+ V VG G +Y VLQ HY V
Sbjct: 124 C-------SAGTCNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKA 176
Query: 388 FREPNSEPDESGILLKIQHKPTKKLAGLYLLVTDGSI--PGHSTVFMEAACSYTGKVALH 561
F++ + D +G+ ++I + +AG+YL ++ ++ PG V + AC Y + +H
Sbjct: 177 FQDKHK--DCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYN-RPTIH 233
Query: 562 PFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQEEQMFYPVKDNIKIQPGDVLASRCVM 741
PFAYRVHTH LG++VSGF V WT IG++SPQ Q FYPV+ ++I PGD++A+RC+
Sbjct: 234 PFAYRVHTHQLGQVVSGFRVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLF 293
Query: 742 ENKGR 756
KGR
Sbjct: 294 TGKGR 298
>sp|P08478|AMD1_XENLA Peptidyl-glycine alpha-amidating monooxygenase I precursor (Peptide
C-terminal alpha-amidating enzyme I) (AE-I)
Length = 400
Score = 185 bits (470), Expect = 1e-46
Identities = 93/245 (37%), Positives = 143/245 (58%), Gaps = 3/245 (1%)
Frame = +1
Query: 31 LKMPGVRPTKEDEYICHAVDLK-NETFYITGFQPKANATHAHHILIHSCIEPGSMKSSWN 207
++MPGV PT+ D Y+C + L ++ Y+ F+P AN AHH+L+ C P S W+
Sbjct: 62 IRMPGVTPTESDTYLCKSYRLPVDDEAYVVDFRPHANMDTAHHMLLFGCNIPSSTDDYWD 121
Query: 208 CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSIGKQYLVLQSHYKQVDY 387
C + C ++Y+W NA +LP+ V VG G +Y VLQ HY V
Sbjct: 122 C-------SAGTCMDKSSIMYAWAKNAPPTKLPEGVGFRVGGKSGSRYFVLQVHYGNVKA 174
Query: 388 FREPNSEPDESGILLKIQHKPTKKLAGLYLLVTDGSI--PGHSTVFMEAACSYTGKVALH 561
F++ + D +G+ +++ + ++AG+YL ++ ++ PG V + AC Y + +H
Sbjct: 175 FQDKHK--DCTGVTVRVTPEKQPQIAGIYLSMSVDTVIPPGEEAVNSDIACLYN-RPTIH 231
Query: 562 PFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQEEQMFYPVKDNIKIQPGDVLASRCVM 741
PFAYRVHTH LG++VSGF V W+ IG++SPQ Q FYPV+ ++I PGD++A+RC+
Sbjct: 232 PFAYRVHTHQLGQVVSGFRVRHGKWSLIGRQSPQLPQAFYPVEHPVEISPGDIIATRCLF 291
Query: 742 ENKGR 756
KGR
Sbjct: 292 TGKGR 296
>sp|P10731|AMD_BOVIN Peptidyl-glycine alpha-amidating monooxygenase precursor (PAM)
Length = 972
Score = 182 bits (461), Expect = 2e-45
Identities = 96/245 (39%), Positives = 138/245 (56%), Gaps = 3/245 (1%)
Frame = +1
Query: 31 LKMPGVRPTKEDEYICHAVDLK-NETFYITGFQPKANATHAHHILIHSCIEPGSMKSSWN 207
++MPGV P + D Y C +V L +E ++ F+P+A+ HH+L+ C P S + W
Sbjct: 61 IRMPGVTPKQSDTYFCMSVRLPMDEEAFVIDFKPRASMDTVHHMLLFGCNMPASTGNYWF 120
Query: 208 CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSIGKQYLVLQSHYKQVDY 387
C E C ++Y+W NA RLPK V VG G +Y VLQ HY +
Sbjct: 121 CDE-------GTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISA 173
Query: 388 FREPNSEPDESGILLKIQHKPTKKLAGLYLLVTDGSI--PGHSTVFMEAACSYTGKVALH 561
FR+ + D SG+ L + P +AG+YL+++ ++ PG V + +C Y K +H
Sbjct: 174 FRD--NHKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPPGGKVVNSDISCHYK-KYPMH 230
Query: 562 PFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQEEQMFYPVKDNIKIQPGDVLASRCVM 741
FAYRVHTH LGK+VSG+ V WT IG++SPQ Q FYPV+ + + GD+LA+RCV
Sbjct: 231 VFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVSFGDILAARCVF 290
Query: 742 ENKGR 756
+GR
Sbjct: 291 TGEGR 295
>sp|P97467|AMD_MOUSE Peptidyl-glycine alpha-amidating monooxygenase precursor (PAM)
Length = 979
Score = 180 bits (457), Expect = 5e-45
Identities = 95/245 (38%), Positives = 137/245 (55%), Gaps = 3/245 (1%)
Frame = +1
Query: 31 LKMPGVRPTKEDEYICHAVDLK-NETFYITGFQPKANATHAHHILIHSCIEPGSMKSSWN 207
++MPGV P + D Y C ++ L +E ++ F+P+A+ HH+L+ C P S S W
Sbjct: 65 IRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWF 124
Query: 208 CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSIGKQYLVLQSHYKQVDY 387
C E C ++Y+W NA RLPK V VG G +Y VLQ HY +
Sbjct: 125 CDE-------GTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISA 177
Query: 388 FREPNSEPDESGILLKIQHKPTKKLAGLYLLVTDGSI--PGHSTVFMEAACSYTGKVALH 561
FR+ + D SG+ L + P +AG+YL+++ ++ PG V + +C Y +H
Sbjct: 178 FRD--NHKDCSGVSLHLTRVPQPLIAGMYLMMSVNTVIPPGEKVVNSDISCHYK-MYPMH 234
Query: 562 PFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQEEQMFYPVKDNIKIQPGDVLASRCVM 741
FAYRVHTH LGK+VSG+ V WT IG++SPQ Q FYPV+ + + GD+LA+RCV
Sbjct: 235 VFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVAFGDILAARCVF 294
Query: 742 ENKGR 756
+GR
Sbjct: 295 TGEGR 299
>sp|P14925|AMD_RAT Peptidyl-glycine alpha-amidating monooxygenase precursor (PAM)
Length = 976
Score = 178 bits (451), Expect = 2e-44
Identities = 93/245 (37%), Positives = 137/245 (55%), Gaps = 3/245 (1%)
Frame = +1
Query: 31 LKMPGVRPTKEDEYICHAVDLK-NETFYITGFQPKANATHAHHILIHSCIEPGSMKSSWN 207
++MPGV P + D Y C ++ L +E ++ F+P+A+ HH+L+ C P S S W
Sbjct: 66 IRMPGVTPKESDTYFCMSMRLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWF 125
Query: 208 CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSIGKQYLVLQSHYKQVDY 387
C E C ++Y+W NA RLPK V VG G +Y VLQ HY +
Sbjct: 126 CDE-------GTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISA 178
Query: 388 FREPNSEPDESGILLKIQHKPTKKLAGLYLLVTDGSI--PGHSTVFMEAACSYTGKVALH 561
FR+ + D SG+ + + P +AG+YL+++ ++ PG V + +C Y +H
Sbjct: 179 FRD--NHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGEKVVNADISCQYK-MYPMH 235
Query: 562 PFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQEEQMFYPVKDNIKIQPGDVLASRCVM 741
FAYRVHTH LGK+VSG+ V WT IG+++PQ Q FYPV+ + + GD+LA+RCV
Sbjct: 236 VFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYPVEHPVDVTFGDILAARCVF 295
Query: 742 ENKGR 756
+GR
Sbjct: 296 TGEGR 300
>sp|P19021|AMD_HUMAN Peptidyl-glycine alpha-amidating monooxygenase precursor (PAM)
Length = 973
Score = 177 bits (450), Expect = 3e-44
Identities = 97/245 (39%), Positives = 136/245 (55%), Gaps = 3/245 (1%)
Frame = +1
Query: 31 LKMPGVRPTKEDEYICHAVDLK-NETFYITGFQPKANATHAHHILIHSCIEPGSMKSSWN 207
++MPGV P + D Y C ++ + +E ++ F+P+A+ HH+L+ C P S S W
Sbjct: 61 IRMPGVTPKQSDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWF 120
Query: 208 CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSIGKQYLVLQSHYKQVDY 387
C E C ++Y+W NA RLPK V VG G +Y VLQ HY +
Sbjct: 121 CDE-------GTCTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISA 173
Query: 388 FREPNSEPDESGILLKIQHKPTKKLAGLYLLVT-DGSIP-GHSTVFMEAACSYTGKVALH 561
FR+ N D SG+ L + P +AG+YL+++ D IP G V + +C Y +H
Sbjct: 174 FRDNNK--DCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKN-YPMH 230
Query: 562 PFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQEEQMFYPVKDNIKIQPGDVLASRCVM 741
FAYRVHTH LGK+VSG+ V WT IG++SPQ Q FYPV + + GD+LA+RCV
Sbjct: 231 VFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVF 290
Query: 742 ENKGR 756
+GR
Sbjct: 291 TGEGR 295
>sp|P09172|DOPO_HUMAN Dopamine beta-hydroxylase precursor (Dopamine beta-monooxygenase)
Length = 603
Score = 60.5 bits (145), Expect = 7e-09
Identities = 65/273 (23%), Positives = 112/273 (41%), Gaps = 22/273 (8%)
Frame = +1
Query: 4 ISAEAKEVLLKMPGVR-PTKEDEYICHAVDLKN--ETFYITGFQP---KANATHAHHILI 165
+ ++A + ++ P ++ P++E Y C+ +L +I ++P K N HH+ +
Sbjct: 193 LPSDACTMEVQAPNIQIPSQETTYWCYIKELPKGFSRHHIIKYEPIVTKGNEALVHHMEV 252
Query: 166 HSCI-EPGSMKS-SWNCQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSI 339
C E S+ S C D C+ ++ +W + A F P++ + G
Sbjct: 253 FQCAPEMDSVPHFSGPCDSKMKPDRLNYCRH---VLAAWALGAKAFYYPEEAGLAFGGPG 309
Query: 340 GKQYLVLQSHYKQVDYFREPNSEPDESGILLKIQHKPTKKLAGLYLL----VTDGSIPGH 507
+YL L+ HY N D SGI L K + AG+ L +IP
Sbjct: 310 SSRYLRLEVHYHNPLVIEGRN---DSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPR 366
Query: 508 STVFMEAA-----CSYTG--KVALHPFAYRVHTHSLG-KLVSGFVVHRKNWTEIGKKSPQ 663
T F+ C+ +H FA ++HTH G K+V+ V + W + + +
Sbjct: 367 ETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHY 426
Query: 664 EE--QMFYPVKDNIKIQPGDVLASRCVMENKGR 756
Q +K + + PGDVL + C + R
Sbjct: 427 SPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDR 459
>sp|Q05754|DOPO_RAT Dopamine beta-hydroxylase precursor (Dopamine beta-monooxygenase)
Length = 620
Score = 57.0 bits (136), Expect = 8e-08
Identities = 60/273 (21%), Positives = 101/273 (36%), Gaps = 21/273 (7%)
Frame = +1
Query: 1 TISAEAKEVLLKMPGVRPTKEDEYICHAVDL-----KNETFYITGFQPKANATHAHHILI 165
T+ A +VL+ P+ E Y C+ +L ++ + N HH+ +
Sbjct: 216 TMDIRAPDVLI------PSTETTYWCYITELPLHFPRHHIIMYEAIVTEGNEALVHHMEV 269
Query: 166 HSCIEPGSMKSSWN--CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSI 339
C +N C D C+ ++ +W + A F P++ + +G S
Sbjct: 270 FQCTNESEAFPMFNGPCDSKMKPDRLNYCRH---VLAAWALGAKAFYYPEEAGVPLGSSG 326
Query: 340 GKQYLVLQSHYKQVDYFREPNSEPDESGILLKIQHKPTKKLAG---LYLLVTD-GSIPGH 507
++L L+ HY R D SGI L AG L L+ T +IP
Sbjct: 327 SSRFLRLEVHYHNP---RNIQGRRDSSGIRLHYTASLRPNEAGIMELGLVYTPLMAIPPQ 383
Query: 508 STVFMEAA-----CSYTG--KVALHPFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQE 666
T F+ C+ K + FA ++HTH G+ V + E+ +
Sbjct: 384 ETTFVLTGYCTDRCTQMALPKSGIRIFASQLHTHLTGRKVITVLARDGQQREVVNRDNHY 443
Query: 667 EQMFYPV---KDNIKIQPGDVLASRCVMENKGR 756
F + K+ + + GDVL + C + R
Sbjct: 444 SPHFQEIRMLKNAVTVHQGDVLITSCTYNTENR 476
>sp|Q64237|DOPO_MOUSE Dopamine beta-hydroxylase precursor (Dopamine beta-monooxygenase)
Length = 621
Score = 53.1 bits (126), Expect = 1e-06
Identities = 64/274 (23%), Positives = 102/274 (37%), Gaps = 24/274 (8%)
Frame = +1
Query: 1 TISAEAKEVLLKMPGVRPTKEDEYICHAVDL-----KNETFYITGFQPKANATHAHHILI 165
T+ A ++L+ P E Y C+ +L ++ + N HH+ +
Sbjct: 217 TMDIRAPDILI------PDNEQTYWCYITELPPRFPRHHIIMYEAIVTEGNEALVHHMEV 270
Query: 166 HSCIEPGSMKSSWN--CQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSI 339
C +N C D C+ ++ +W + A F PK+ + G
Sbjct: 271 FQCAAESEDFPQFNGPCDSKMKPDRLNYCRH---VLAAWALGAKAFYYPKEAGVPFGGPG 327
Query: 340 GKQYLVLQSHYKQVDYFREPNSEPDESGILLKIQHKPTKKLAG---LYLLVTD-GSIPGH 507
+L L+ HY R+ D SGI L + AG L L+ T +IP
Sbjct: 328 SSPFLRLEVHYHNP---RKIQGRQDSSGIRLPYTATLRRYDAGIMELGLVYTPLMAIPPQ 384
Query: 508 STVFMEAA-----CSYTG--KVALHPFAYRVHTHSLGKLVSGFVV----HRKNWTEIGKK 654
T F+ C+ +H FA ++HTH G+ V + RK
Sbjct: 385 ETAFVLTGYCTDKCTQMALQDSGIHIFASQLHTHLTGRKVVTVLARDGQERKEVNRDNHY 444
Query: 655 SPQEEQMFYPVKDNIKIQPGDVLASRCV--MENK 750
SP ++ +K + + PGDVL + C ENK
Sbjct: 445 SPHFREI-RMLKKVVTVYPGDVLITSCTYNTENK 477
>sp|P15101|DOPO_BOVIN Dopamine beta-hydroxylase precursor (Dopamine beta-monooxygenase)
Length = 610
Score = 52.8 bits (125), Expect = 1e-06
Identities = 61/273 (22%), Positives = 102/273 (37%), Gaps = 21/273 (7%)
Frame = +1
Query: 1 TISAEAKEVLLKMPGVRPTKEDEYICHAVDLKN--ETFYITGFQP---KANATHAHHILI 165
T+ A +VL+ P ++ Y C+ +L + +I ++P + N HH+ +
Sbjct: 206 TMEIRAPDVLI------PGQQTTYWCYVTELPDGFPRHHIVMYEPIVTEGNEALVHHMEV 259
Query: 166 HSCIEP--GSMKSSWNCQEMKSKDAQPVCKSGEKLIYSWGMNASGFRLPKDVSIMVGKSI 339
C S C C+ ++ +W + A F P++ + G
Sbjct: 260 FQCAAEFRDHPHFSGPCDSKMKPQRLNFCRH---VLAAWALGAKAFYYPEEAGLAFGGPG 316
Query: 340 GKQYLVLQSHYKQVDYFREPNSEPDESGILLKIQHKPTKKLAGLYLL----VTDGSIPGH 507
++L L+ HY D SGI L + AG+ L +IP
Sbjct: 317 SSRFLRLEVHYHNPLVI---TGRRDSSGIRLYYTAALRRFDAGIMELGLAYTPVMAIPPQ 373
Query: 508 STVFMEAA-----CSYTGKVA--LHPFAYRVHTHSLGKLVSGFVVHRKNWTEIGKKSPQE 666
T F+ C+ A +H FA ++HTH G+ V + TEI +
Sbjct: 374 ETAFVLTGYCTDKCTQLALPASGIHIFASQLHTHLTGRKVVTVLARDGRETEIVNRDNHY 433
Query: 667 EQMFYPV---KDNIKIQPGDVLASRCVMENKGR 756
F + K + +QPGDVL + C + R
Sbjct: 434 SPHFQEIRMLKKVVSVQPGDVLITSCTYNTEDR 466
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 120,298,940
Number of Sequences: 369166
Number of extensions: 2707554
Number of successful extensions: 6478
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6453
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9750928390
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)