Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_L09
(933 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P05208|ELA2_MOUSE Elastase-2 precursor 95 3e-19
sp|P08419|ELA2_PIG Elastase-2 precursor 95 3e-19
sp|P00774|ELA2_RAT Elastase-2 precursor 86 1e-16
sp|P06868|PLMN_BOVIN Plasminogen precursor [Contains: Plasm... 86 2e-16
sp|P08218|ELA2B_HUMAN Elastase-2B precursor 85 3e-16
sp|Q867B0|ELA1_CANFA Elastase-1 precursor 84 4e-16
sp|Q9UNI1|ELA1_HUMAN Elastase-1 precursor 83 1e-15
sp|P08217|ELA2A_HUMAN Elastase-2A precursor 82 2e-15
sp|Q29461|ELA2_BOVIN Elastase-2 precursor (Elastase II) 81 4e-15
sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Mat... 80 8e-15
>sp|P05208|ELA2_MOUSE Elastase-2 precursor
Length = 271
Score = 94.7 bits (234), Expect = 3e-19
Identities = 54/177 (30%), Positives = 86/177 (48%), Gaps = 3/177 (1%)
Frame = +1
Query: 229 VNCGIKPMEIEN---KIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAA 399
++CG E+E+ +++GG+++ N WPW V L V R + CGG+L+ WV+TAA
Sbjct: 15 LSCGYPTYEVEDDVSRVVGGQEATPNTWPWQVSLQVLSSGRWRHNCGGSLVANNWVLTAA 74
Query: 400 HCLKTIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFR 579
HCL Y + V++ H + A + ++S +++ +
Sbjct: 75 HCLSN--------------------YQTYRVLLGAHSLSNPGAGSAAVQVSKLVVHQRWN 114
Query: 580 ADYSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
+ N YDIAL+KL PV SK NI C+P L ++ CY GWG ++ N
Sbjct: 115 SQNVGNGYDIALIKLASPVTLSK--NIQTACLPPA-GTILPRNYVCYVTGWGLLQTN 168
>sp|P08419|ELA2_PIG Elastase-2 precursor
Length = 269
Score = 94.7 bits (234), Expect = 3e-19
Identities = 54/176 (30%), Positives = 89/176 (50%), Gaps = 2/176 (1%)
Frame = +1
Query: 229 VNCGIKPMEIEN--KIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAH 402
++CG+ P + +++GGED+R N+WPW V L + + + CGGTL++ WV+TAAH
Sbjct: 15 LSCGL-PANLPQLPRVVGGEDARPNSWPWQVSLQYDSSGQWRHTCGGTLVDQSWVLTAAH 73
Query: 403 CLKTIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRA 582
C+ + + VV+ H +T++ ++ K+S +++ + +
Sbjct: 74 CISSSRT--------------------YRVVLGRHSLSTNEPGSLAVKVSKLVVHQDWNS 113
Query: 583 DYSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
+ SN DIALLKL PV S I C+P L + CY GWG ++ N
Sbjct: 114 NQLSNGNDIALLKLASPV--SLTDKIQLGCLP-AAGTILPNNYVCYVTGWGRLQTN 166
>sp|P00774|ELA2_RAT Elastase-2 precursor
Length = 271
Score = 85.9 bits (211), Expect = 1e-16
Identities = 49/177 (27%), Positives = 88/177 (49%), Gaps = 3/177 (1%)
Frame = +1
Query: 229 VNCGIKPMEIEN---KIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAA 399
++CG E+++ +++GG+++ N+WPW V L + + CGG+L+ WV+TAA
Sbjct: 15 LSCGYPTYEVQHDVSRVVGGQEASPNSWPWQVSLQYLSSGKWHHTCGGSLVANNWVLTAA 74
Query: 400 HCLKTIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFR 579
HC+ ++R Y V++ H +T ++ ++ ++S +++ +
Sbjct: 75 HCIS--------------NSRTY------RVLLGRHSLSTSESGSLAVQVSKLVVHEKWN 114
Query: 580 ADYSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
A SN DIAL+KL PV + I C+P L + CY GWG ++ N
Sbjct: 115 AQKLSNGNDIALVKLASPV--ALTSKIQTACLPPA-GTILPNNYPCYVTGWGRLQTN 168
>sp|P06868|PLMN_BOVIN Plasminogen precursor [Contains: Plasmin heavy chain A; Activation
peptide; Plasmin heavy chain A, short form; Plasmin
light chain B]
Length = 812
Score = 85.5 bits (210), Expect = 2e-16
Identities = 55/174 (31%), Positives = 85/174 (48%), Gaps = 3/174 (1%)
Frame = +1
Query: 232 NCG---IKPMEIENKIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAH 402
+CG ++P + +I+GG S+ ++WPW V L R+ S + CGGTLI+P WV+TAAH
Sbjct: 569 DCGKPKVEPKKCSGRIVGGCVSKPHSWPWQVSL----RRSSRHFCGGTLISPKWVLTAAH 624
Query: 403 CLKTIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRA 582
CL I A++ + V++ H+ + + + ++ LFR
Sbjct: 625 CLDNILALSF-----------------YKVILGAHNEKVRE-----QSVQEIPVSRLFRE 662
Query: 583 DYSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIE 744
+ DIALLKL +P I +K V P NY + +CY GWG +
Sbjct: 663 PSQA---DIALLKLSRPAIITKEV---IPACLPPPNYMVAARTECYITGWGETQ 710
>sp|P08218|ELA2B_HUMAN Elastase-2B precursor
Length = 269
Score = 84.7 bits (208), Expect = 3e-16
Identities = 48/175 (27%), Positives = 85/175 (48%), Gaps = 1/175 (0%)
Frame = +1
Query: 229 VNCGIKPMEIE-NKIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAHC 405
++CG+ + ++++GGE++R N+WPW V L + + + CGG+LI WV+TAAHC
Sbjct: 15 LSCGVSTYAPDMSRMLGGEEARPNSWPWQVSLQYSSNGQWYHTCGGSLIANSWVLTAAHC 74
Query: 406 LKTIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRAD 585
+ + + V++ H+ ++ ++ +S +++ + ++
Sbjct: 75 ISSSRI--------------------YRVMLGQHNLYVAESGSLAVSVSKIVVHKDWNSN 114
Query: 586 YSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
S DIALLKL PV S I C+P L + CY GWG ++ N
Sbjct: 115 QVSKGNDIALLKLANPV--SLTDKIQLACLPPA-GTILPNNYPCYVTGWGRLQTN 166
>sp|Q867B0|ELA1_CANFA Elastase-1 precursor
Length = 258
Score = 84.3 bits (207), Expect = 4e-16
Identities = 50/166 (30%), Positives = 82/166 (49%)
Frame = +1
Query: 253 EIENKIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAHCLKTIEAVNS 432
E +++GG ++R+N+WP + L + + CGGTLI WV+TAAHC+
Sbjct: 14 ETNARVVGGTEARRNSWPSQISLQYLSGGKWYHTCGGTLIRQNWVMTAAHCV-------- 65
Query: 433 VPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRADYSSNLYDIA 612
D F VVI +H+ + +D E S + +++P + ++ S YDIA
Sbjct: 66 ------------DRTMTFRVVIGEHNLSQNDGTEQSASVQKIVVHPYWNSNDVSAGYDIA 113
Query: 613 LLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
LL+L + V + V + +PQ + L ++ CY GWG + N
Sbjct: 114 LLRLAQKVTLNSYVQLG--VLPQ-EGAILANNSPCYITGWGLTKTN 156
>sp|Q9UNI1|ELA1_HUMAN Elastase-1 precursor
Length = 258
Score = 82.8 bits (203), Expect = 1e-15
Identities = 48/166 (28%), Positives = 78/166 (46%)
Frame = +1
Query: 253 EIENKIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAHCLKTIEAVNS 432
E +++GG ++ +N+WP + L + CGGTLI WV+TAAHC+
Sbjct: 14 ETNARVVGGTEAGRNSWPSQISLQYRSGGSRYHTCGGTLIRQNWVMTAAHCV-------- 65
Query: 433 VPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRADYSSNLYDIA 612
D F VV DH+ + +D E + +++P + +D + YDIA
Sbjct: 66 ------------DYQKTFRVVAGDHNLSQNDGTEQYVSVQKIVVHPYWNSDNVAAGYDIA 113
Query: 613 LLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
LL+L + V + V + +PQ + L ++ CY GWG + N
Sbjct: 114 LLRLAQSVTLNSYVQLG--VLPQ-EGAILANNSPCYITGWGKTKTN 156
>sp|P08217|ELA2A_HUMAN Elastase-2A precursor
Length = 269
Score = 82.4 bits (202), Expect = 2e-15
Identities = 46/162 (28%), Positives = 77/162 (47%)
Frame = +1
Query: 265 KIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAHCLKTIEAVNSVPLG 444
+++GGE++R N+WPW V L + + + CGG+LI WV+TAAHC+ +
Sbjct: 28 RVVGGEEARPNSWPWQVSLQYSSNGKWYHTCGGSLIANSWVLTAAHCISSSRT------- 80
Query: 445 EMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRADYSSNLYDIALLKL 624
+ V + H+ ++ ++ +S +++ + ++ S DIALLKL
Sbjct: 81 -------------YRVGLGRHNLYVAESGSLAVSVSKIVVHKDWNSNQISKGNDIALLKL 127
Query: 625 DKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
PV S I C+P L + CY GWG ++ N
Sbjct: 128 ANPV--SLTDKIQLACLPPA-GTILPNNYPCYVTGWGRLQTN 166
>sp|Q29461|ELA2_BOVIN Elastase-2 precursor (Elastase II)
Length = 269
Score = 81.3 bits (199), Expect = 4e-15
Identities = 47/175 (26%), Positives = 85/175 (48%), Gaps = 1/175 (0%)
Frame = +1
Query: 229 VNCGIKPMEIE-NKIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAHC 405
++CG+ + ++++GGED+R N+WPW V L + + + CGG+LI WV+TAAHC
Sbjct: 15 LSCGVPTYPPQLSRVVGGEDARPNSWPWQVSLQYSSSGQWRHTCGGSLIEQNWVLTAAHC 74
Query: 406 LKTIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRAD 585
+ + + VV+ +T ++ ++ +S +I+ + ++
Sbjct: 75 ISSSRT--------------------YRVVVGRQSLSTVESGSLTIAVSKSVIHEKWNSN 114
Query: 586 YSSNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQN 750
+ DIALLKL V + + + C+P L + CY GWG ++ N
Sbjct: 115 QLAQGNDIALLKLASSVPLTDKIQLG--CLP-AAGTILPNNYVCYVTGWGRLQSN 166
>sp|Q8IU80|TMPS6_HUMAN Transmembrane protease, serine 6 (Matriptase-2)
Length = 802
Score = 80.1 bits (196), Expect = 8e-15
Identities = 53/175 (30%), Positives = 84/175 (48%)
Frame = +1
Query: 232 NCGIKPMEIENKIIGGEDSRKNAWPWHVGLYVNVRKRSIYICGGTLINPGWVVTAAHCLK 411
+CG++ ++I+GG S + WPW L V R +ICGG LI WV+TAAHC +
Sbjct: 558 DCGLQGPS--SRIVGGAVSSEGEWPWQASLQVRGR----HICGGALIADRWVITAAHCFQ 611
Query: 412 TIEAVNSVPLGEMIDTRKYDLYDKFHVVINDHDRTTDDADEISRKLSYFIINPLFRADYS 591
++V L+ F + + R E+S K+S +++P D
Sbjct: 612 EDSMASTV------------LWTVFLGKVWQNSRW---PGEVSFKVSRLLLHPYHEED-- 654
Query: 592 SNLYDIALLKLDKPVIPSKVVNINFPCVPQVKNYTLDKSAKCYAIGWGNIEQNEP 756
S+ YD+ALL+LD PV+ S V P +++ + C+ GWG + + P
Sbjct: 655 SHDYDVALLQLDHPVVRSAAVR---PVCLPARSHFFEPGLHCWITGWGALREGGP 706
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 106,155,055
Number of Sequences: 369166
Number of extensions: 2186323
Number of successful extensions: 6695
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5977
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6294
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9606826000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)