Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_K24
(621 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q01081|U2AF1_HUMAN Splicing factor U2AF 35 kDa subunit (... 157 2e-38
sp|Q9D883|U2AF1_MOUSE Splicing factor U2AF 35 kDa subunit (... 157 2e-38
sp|Q94535|U2AF1_DROME Splicing factor U2af 38 kDa subunit (... 150 3e-36
sp|Q09176|U2AF1_SCHPO Splicing factor U2AF 23 kDa subunit (... 114 2e-25
sp|Q62377|U2AFM_MOUSE U2 small nuclear ribonucleoprotein au... 72 1e-12
sp|Q15696|U2AFM_HUMAN U2 small nuclear ribonucleoprotein au... 70 3e-12
sp|Q15695|U2AFL_HUMAN U2 small nuclear ribonucleoprotein au... 70 3e-12
sp|Q64707|U2AFL_MOUSE U2 small nuclear ribonucleoprotein au... 70 6e-12
sp|Q8JZX4|SPF45_MOUSE Splicing factor 45 (45 kDa splicing f... 38 0.024
sp|Q96I25|SPF45_HUMAN Splicing factor 45 (45 kDa splicing f... 38 0.024
>sp|Q01081|U2AF1_HUMAN Splicing factor U2AF 35 kDa subunit (U2 auxiliary factor 35 kDa
subunit) (U2 snRNP auxiliary factor small subunit)
Length = 240
Score = 157 bits (398), Expect = 2e-38
Identities = 70/79 (88%), Positives = 75/79 (94%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HLVGNVY+KFRREEDAEKAV +LNNRWF G+P+HAELSPVTDFREACC QYE GECTRGG
Sbjct: 108 HLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGG 167
Query: 374 FCNFMHLKPISRELRRKLY 430
FCNFMHLKPISRELRR+LY
Sbjct: 168 FCNFMHLKPISRELRRELY 186
Score = 35.8 bits (81), Expect = 0.090
Identities = 22/64 (34%), Positives = 29/64 (45%)
Frame = +3
Query: 3 LQNLYIAPQNTAQTADGSHSNFSVSMSEVEAXXXXXXXXXXXXIXXXXXXXXXXXMNVCD 182
L N+Y PQN++Q+ADG ++S+VE MNVCD
Sbjct: 48 LLNIYRNPQNSSQSADG----LRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCD 103
Query: 183 NLGD 194
NLGD
Sbjct: 104 NLGD 107
>sp|Q9D883|U2AF1_MOUSE Splicing factor U2AF 35 kDa subunit (U2 auxiliary factor 35 kDa
subunit) (U2 snRNP auxiliary factor small subunit)
Length = 239
Score = 157 bits (398), Expect = 2e-38
Identities = 70/79 (88%), Positives = 75/79 (94%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HLVGNVY+KFRREEDAEKAV +LNNRWF G+P+HAELSPVTDFREACC QYE GECTRGG
Sbjct: 108 HLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGG 167
Query: 374 FCNFMHLKPISRELRRKLY 430
FCNFMHLKPISRELRR+LY
Sbjct: 168 FCNFMHLKPISRELRRELY 186
Score = 35.8 bits (81), Expect = 0.090
Identities = 22/64 (34%), Positives = 29/64 (45%)
Frame = +3
Query: 3 LQNLYIAPQNTAQTADGSHSNFSVSMSEVEAXXXXXXXXXXXXIXXXXXXXXXXXMNVCD 182
L N+Y PQN++Q+ADG ++S+VE MNVCD
Sbjct: 48 LLNIYRNPQNSSQSADG----LRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCD 103
Query: 183 NLGD 194
NLGD
Sbjct: 104 NLGD 107
>sp|Q94535|U2AF1_DROME Splicing factor U2af 38 kDa subunit (U2 auxiliary factor 38 kDa
subunit) (U2 snRNP auxiliary factor small subunit)
Length = 264
Score = 150 bits (379), Expect = 3e-36
Identities = 68/80 (85%), Positives = 72/80 (90%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HLVGNVYIKFR E DAEKA +LNNRWFGGRPV++ELSPVTDFREACC QYE GECTR G
Sbjct: 110 HLVGNVYIKFRNEADAEKAANDLNNRWFGGRPVYSELSPVTDFREACCRQYEMGECTRSG 169
Query: 374 FCNFMHLKPISRELRRKLYA 433
FCNFMHLKPISRELRR LY+
Sbjct: 170 FCNFMHLKPISRELRRYLYS 189
Score = 45.8 bits (107), Expect = 9e-05
Identities = 25/64 (39%), Positives = 33/64 (51%)
Frame = +3
Query: 3 LQNLYIAPQNTAQTADGSHSNFSVSMSEVEAXXXXXXXXXXXXIXXXXXXXXXXXMNVCD 182
LQNLY+ PQN+A++ADGSH ++S+ E + MNVCD
Sbjct: 48 LQNLYVNPQNSAKSADGSH--LVANVSDEEMQEHYDNFFEDVFVECEDKYGEIEEMNVCD 105
Query: 183 NLGD 194
NLGD
Sbjct: 106 NLGD 109
>sp|Q09176|U2AF1_SCHPO Splicing factor U2AF 23 kDa subunit (U2 auxiliary factor 23 kDa
subunit) (U2 snRNP auxiliary factor small subunit)
(U2AF23)
Length = 216
Score = 114 bits (286), Expect = 2e-25
Identities = 50/78 (64%), Positives = 61/78 (78%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HLVGNVY++F+ EE A+ A+ +LN+RW+ RPV+AELSPVTDFREACC Q+E EC RGG
Sbjct: 102 HLVGNVYVRFKYEESAQNAIDDLNSRWYSQRPVYAELSPVTDFREACCRQHETSECQRGG 161
Query: 374 FCNFMHLKPISRELRRKL 427
CNFMH K S +L R L
Sbjct: 162 LCNFMHAKKPSPQLLRDL 179
>sp|Q62377|U2AFM_MOUSE U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 2 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 2)
Length = 462
Score = 71.6 bits (174), Expect = 1e-12
Identities = 29/67 (43%), Positives = 43/67 (64%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HL GNVY++++ EED + A + N RW+ GR + E PVT ++ A C +E +C RG
Sbjct: 269 HLRGNVYVQYQSEEDCQAAFSVFNGRWYAGRQLQCEFCPVTRWKMAICGLFEVQQCPRGK 328
Query: 374 FCNFMHL 394
CNF+H+
Sbjct: 329 HCNFLHV 335
>sp|Q15696|U2AFM_HUMAN U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 2 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 2)
Length = 482
Score = 70.5 bits (171), Expect = 3e-12
Identities = 28/67 (41%), Positives = 44/67 (65%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HL GNVY++++ EE+ + A++ N RW+ GR + E PVT ++ A C +E +C RG
Sbjct: 265 HLRGNVYVQYQSEEECQAALSLFNGRWYAGRQLQCEFCPVTRWKMAICGLFEIQQCPRGK 324
Query: 374 FCNFMHL 394
CNF+H+
Sbjct: 325 HCNFLHV 331
>sp|Q15695|U2AFL_HUMAN U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 1 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 1)
Length = 479
Score = 70.5 bits (171), Expect = 3e-12
Identities = 28/67 (41%), Positives = 44/67 (65%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HL GNVY++++ EE+ + A++ N RW+ GR + E PVT ++ A C +E +C RG
Sbjct: 270 HLRGNVYVQYQSEEECQAALSLFNGRWYAGRQLQCEFCPVTRWKMAICGLFEIQQCPRGK 329
Query: 374 FCNFMHL 394
CNF+H+
Sbjct: 330 HCNFLHV 336
>sp|Q64707|U2AFL_MOUSE U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit
related-protein 1 (U2(RNU2) small nuclear RNA auxillary
factor 1-like 1) (SP2)
Length = 428
Score = 69.7 bits (169), Expect = 6e-12
Identities = 32/83 (38%), Positives = 51/83 (61%), Gaps = 4/83 (4%)
Frame = +2
Query: 194 HLVGNVYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFREACCTQYENGECTRGG 373
HL GNVY++++ EE+ + A++ N RW+ GR + E PVT ++ A C +E +C +G
Sbjct: 256 HLRGNVYVQYQSEEECQAALSLFNGRWYAGRQLQCEFCPVTRWKVAICGLFEMQKCPKGK 315
Query: 374 FCNFMHL--KPIS--RELRRKLY 430
CNF+H+ P + RE R +Y
Sbjct: 316 HCNFLHVFRNPNNEFREANRDIY 338
>sp|Q8JZX4|SPF45_MOUSE Splicing factor 45 (45 kDa splicing factor) (RNA-binding motif
protein 17)
Length = 405
Score = 37.7 bits (86), Expect = 0.024
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +2
Query: 209 VYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFR 325
++++F R E A KAV +LN R+FGGR V A + FR
Sbjct: 359 IFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKFR 397
>sp|Q96I25|SPF45_HUMAN Splicing factor 45 (45 kDa splicing factor) (RNA-binding motif
protein 17)
Length = 401
Score = 37.7 bits (86), Expect = 0.024
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +2
Query: 209 VYIKFRREEDAEKAVTELNNRWFGGRPVHAELSPVTDFR 325
++++F R E A KAV +LN R+FGGR V A + FR
Sbjct: 355 IFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKFR 393
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,878,043
Number of Sequences: 369166
Number of extensions: 1242152
Number of successful extensions: 3003
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2862
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2999
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4877307000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)