Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_001_J24 (284 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q8BSF4|PISD_MOUSE Phosphatidylserine decarboxylase proen... 64 1e-10 sp|P27465|PISD_CRIGR Phosphatidylserine decarboxylase proen... 64 1e-10 sp|Q9UG56|PISD_HUMAN Phosphatidylserine decarboxylase proen... 64 1e-10 sp|Q5R8I8|PISD_PONPY Phosphatidylserine decarboxylase proen... 64 1e-10 sp|P39006|PSD1_YEAST Phosphatidylserine decarboxylase proen... 53 2e-07 sp|Q10949|PISD_CAEEL Putative phosphatidylserine decarboxyl... 43 2e-04 sp|Q6F6W3|PSD_ACIAD Phosphatidylserine decarboxylase proenz... 37 0.014 sp|P53888|YNR0_YEAST Hypothetical 14.7 kDa protein in RPS3-... 36 0.024 sp|O25911|PSD_HELPY Phosphatidylserine decarboxylase proenz... 35 0.041 sp|Q9ZJN0|PSD_HELPJ Phosphatidylserine decarboxylase proenz... 35 0.070
>sp|Q8BSF4|PISD_MOUSE Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 406 Score = 63.9 bits (154), Expect = 1e-10 Identities = 29/46 (63%), Positives = 35/46 (76%) Frame = +1 Query: 4 GIPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 GIP+ KG+ GEFN GSTIVLIFEAP DF F ++ ++FGEALG Sbjct: 359 GIPMRKGEPLGEFNLGSTIVLIFEAPKDFNFRLKAGQKIRFGEALG 404
>sp|P27465|PISD_CRIGR Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 409 Score = 63.9 bits (154), Expect = 1e-10 Identities = 29/46 (63%), Positives = 35/46 (76%) Frame = +1 Query: 4 GIPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 GIP+ KG+ GEFN GSTIVLIFEAP DF F ++ ++FGEALG Sbjct: 362 GIPMRKGEHLGEFNLGSTIVLIFEAPKDFNFRLKAGQKIRFGEALG 407
>sp|Q9UG56|PISD_HUMAN Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 408 Score = 63.5 bits (153), Expect = 1e-10 Identities = 28/46 (60%), Positives = 35/46 (76%) Frame = +1 Query: 4 GIPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 G+P+ KG+ GEFN GSTIVLIFEAP DF F ++ ++FGEALG Sbjct: 361 GVPMRKGEHLGEFNLGSTIVLIFEAPKDFNFQLKTGQKIRFGEALG 406
>sp|Q5R8I8|PISD_PONPY Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 409 Score = 63.5 bits (153), Expect = 1e-10 Identities = 28/46 (60%), Positives = 35/46 (76%) Frame = +1 Query: 4 GIPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 G+P+ KG+ GEFN GSTIVLIFEAP DF F ++ ++FGEALG Sbjct: 362 GVPMRKGEHLGEFNLGSTIVLIFEAPKDFNFQLKTGQKIRFGEALG 407
>sp|P39006|PSD1_YEAST Phosphatidylserine decarboxylase proenzyme 1, mitochondrial precursor [Contains: Phosphatidylserine decarboxylase beta chain; Phosphatidylserine decarboxylase alpha chain] Length = 500 Score = 53.1 bits (126), Expect = 2e-07 Identities = 24/46 (52%), Positives = 31/46 (67%) Frame = +1 Query: 4 GIPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 G+P+ KG+ G F GST+VL FEAP +F+F V V VK G+ LG Sbjct: 447 GMPLVKGEEMGGFELGSTVVLCFEAPTEFKFDVRVGDKVKMGQKLG 492
>sp|Q10949|PISD_CAEEL Putative phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 377 Score = 43.1 bits (100), Expect = 2e-04 Identities = 17/43 (39%), Positives = 28/43 (65%) Frame = +1 Query: 10 PIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEAL 138 P G+ GEF GSTIVL+F+AP +F ++ +++G++L Sbjct: 330 PYVSGERVGEFRLGSTIVLVFQAPPTIKFAIKAGDPLRYGQSL 372
>sp|Q6F6W3|PSD_ACIAD Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 285 Score = 37.0 bits (84), Expect = 0.014 Identities = 21/49 (42%), Positives = 29/49 (59%), Gaps = 3/49 (6%) Frame = +1 Query: 7 IPIEKGDLFGEFNFGSTIVLIFEAPA---DFQFGVEVNSIVKFGEALGR 144 + ++KGD G F GST +++FE D QF + NSIV GE LG+ Sbjct: 235 VTLQKGDELGRFYLGSTAIILFEKDKMVWDQQF--KANSIVVMGEKLGQ 281
>sp|P53888|YNR0_YEAST Hypothetical 14.7 kDa protein in RPS3-PSD1 intergenic region Length = 131 Score = 36.2 bits (82), Expect = 0.024 Identities = 20/46 (43%), Positives = 22/46 (47%) Frame = -1 Query: 140 PRASPNFTIELTSTPNWKSAGASKIKTIVEPKLNSPNKSPFSIGIP 3 P P T+ T T N S GASK T V P P SP + GIP Sbjct: 74 PNFCPILTLSPTLTSNLNSVGASKQSTTVLPNSKPPISSPLTKGIP 119
>sp|O25911|PSD_HELPY Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 267 Score = 35.4 bits (80), Expect = 0.041 Identities = 22/45 (48%), Positives = 28/45 (62%) Frame = +1 Query: 7 IPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 I ++KGD G F GSTIVL + A F+ E N VKFGE++G Sbjct: 221 IKVKKGDNLGNFEMGSTIVLFIQNTA-FKDLKEKN--VKFGESIG 262
>sp|Q9ZJN0|PSD_HELPJ Phosphatidylserine decarboxylase proenzyme [Contains: Phosphatidylserine decarboxylase alpha chain; Phosphatidylserine decarboxylase beta chain] Length = 267 Score = 34.7 bits (78), Expect = 0.070 Identities = 20/45 (44%), Positives = 25/45 (55%) Frame = +1 Query: 7 IPIEKGDLFGEFNFGSTIVLIFEAPADFQFGVEVNSIVKFGEALG 141 I ++KGD G F GSTIVL + A F VKFGE++G Sbjct: 221 IKVKKGDNLGNFEMGSTIVLFIQNTA---FKDLKEKSVKFGESIG 262
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 29,333,126 Number of Sequences: 369166 Number of extensions: 470266 Number of successful extensions: 1159 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 1151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1158 length of database: 68,354,980 effective HSP length: 64 effective length of database: 56,531,940 effective search space used: 1695958200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)