Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_001_H06 (327 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Incl... 32 0.34 sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Inc... 32 0.45 sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynuc... 31 0.76 sp|P87000|ACU15_NEUCR Transcriptional activator protein acu-15 30 2.2 sp|P35194|YBA4_YEAST Hypothetical 287.5 kDa protein in PDR3... 28 8.4
>sp|O55236|MCE1_MOUSE mRNA capping enzyme (HCE) (MCE1) [Includes: Polynucleotide 5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (GTP--RNA guanylyltransferase) (GTase)] Length = 597 Score = 32.3 bits (72), Expect = 0.34 Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 4/44 (9%) Frame = +1 Query: 16 TLNEALGIFSKARPPGIYDVKYLQQLVKLF----QPSTPIIYPD 135 ++ A+ F++ARPPGIY YL++L + + + P + PD Sbjct: 148 SIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPVLPD 191
>sp|O60942|MCE1_HUMAN mRNA capping enzyme (HCE) (HCAP1) [Includes: Polynucleotide 5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (GTP--RNA guanylyltransferase) (GTase)] Length = 597 Score = 32.0 bits (71), Expect = 0.45 Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 4/44 (9%) Frame = +1 Query: 16 TLNEALGIFSKARPPGIYDVKYLQQLVKLF----QPSTPIIYPD 135 ++ A+ F++ARPPGIY YL++L + + + P + PD Sbjct: 148 SIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPLLPD 191
>sp|Q17607|MCE1_CAEEL mRNA capping enzyme [Includes: Polynucleotide 5'-triphosphatase (mRNA 5'-triphosphatase) (TPase); mRNA guanylyltransferase (GTP--RNA guanylyltransferase) (GTase)] Length = 623 Score = 31.2 bits (69), Expect = 0.76 Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = +1 Query: 19 LNEALGIFSKARPPGIYDVKYLQQLVKLFQPS--TPIIYPDPPKW 147 L+ A+G F++ R GIY Y+ L + P+ I+ P+ P W Sbjct: 159 LDAAIGEFAENRQKGIYKQDYIDDLFARYDPTEDDKILAPEKPDW 203
>sp|P87000|ACU15_NEUCR Transcriptional activator protein acu-15 Length = 926 Score = 29.6 bits (65), Expect = 2.2 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 19 LNEALGIFSKARPPGIYDVKYLQQLVKLFQPSTPIIYPDPPK 144 +N + I +K PG YD K + ++ + S P P PP+ Sbjct: 570 VNSVVKIVTKVNAPGSYDFKRIAGMLVTVEESLPQSLPTPPR 611
>sp|P35194|YBA4_YEAST Hypothetical 287.5 kDa protein in PDR3-HTA2 intergenic region Length = 2493 Score = 27.7 bits (60), Expect = 8.4 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = +2 Query: 194 LLNYQPNLKNLMKCVKFVNQNTTFLLPEGKEN 289 L Y+ NLKNL+ F ++ TTFL G ++ Sbjct: 881 LNKYRDNLKNLLDDTLFKDEITTFLTENGSQS 912
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 32,856,117 Number of Sequences: 369166 Number of extensions: 508112 Number of successful extensions: 1261 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 1247 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1261 length of database: 68,354,980 effective HSP length: 77 effective length of database: 54,130,385 effective search space used: 1678041935 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)