Planarian EST Database


Dr_sW_001_G01

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_001_G01
         (587 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P22061|PIMT_HUMAN  Protein-L-isoaspartate(D-aspartate) O-...   107   2e-23
sp|P80895|PIMT_PIG  Protein-L-isoaspartate(D-aspartate) O-me...   106   4e-23
sp|P22062|PIMT_RAT  Protein-L-isoaspartate(D-aspartate) O-me...   106   5e-23
sp|Q92047|PIMT_BRARE  Protein-L-isoaspartate(D-aspartate) O-...   105   8e-23
sp|P23506|PIMT_MOUSE  Protein-L-isoaspartate(D-aspartate) O-...   105   1e-22
sp|P15246|PIMT_BOVIN  Protein-L-isoaspartate(D-aspartate) O-...   104   2e-22
sp|Q43209|PIMT_WHEAT  Protein-L-isoaspartate O-methyltransfe...   101   2e-21
sp|Q27869|PIMT_DROME  Protein-L-isoaspartate(D-aspartate) O-...    99   8e-21
sp|Q42539|PIMT_ARATH  Protein-L-isoaspartate O-methyltransfe...    98   1e-20
sp|Q6NCU3|PIMT_RHOPA  Protein-L-isoaspartate O-methyltransfe...    79   1e-14
>sp|P22061|PIMT_HUMAN Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (Protein L-isoaspartyl/D-aspartyl methyltransferase)
           (L-isoaspartyl protein carboxyl methyltransferase)
          Length = 227

 Score =  107 bits (267), Expect = 2e-23
 Identities = 56/94 (59%), Positives = 64/94 (68%)
 Frame = +1

Query: 217 SLLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGY 396
           +LL S  +Q    DGR G+ E APYDAIHVGAAA  +P  LI+QLK GGR I PVG  G 
Sbjct: 129 TLLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGG 188

Query: 397 NQSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQ 498
           NQ L Q DK  DGS + K +MGV YVPLTD +KQ
Sbjct: 189 NQMLEQYDKLQDGSIKMKPLMGVIYVPLTDKEKQ 222

 Score = 31.6 bits (70), Expect = 1.5
 Identities = 12/22 (54%), Positives = 17/22 (77%)
 Frame = +3

Query: 135 GKAIGVDHVNDLVKTSIDNCKK 200
           GK IG+DH+ +LV  SI+N +K
Sbjct: 104 GKVIGIDHIKELVDDSINNVRK 125
>sp|P80895|PIMT_PIG Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (Protein L-isoaspartyl/D-aspartyl methyltransferase)
           (L-isoaspartyl protein carboxyl methyltransferase)
          Length = 227

 Score =  106 bits (265), Expect = 4e-23
 Identities = 56/93 (60%), Positives = 63/93 (67%)
 Frame = +1

Query: 220 LLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGYN 399
           LL S  +Q    DGR G+ E APYDAIHVGAAA  +P  LI+QLK GGR I PVG  G N
Sbjct: 130 LLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGN 189

Query: 400 QSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQ 498
           Q L Q DK  DGS + K +MGV YVPLTD +KQ
Sbjct: 190 QMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

 Score = 35.0 bits (79), Expect = 0.14
 Identities = 14/26 (53%), Positives = 19/26 (73%)
 Frame = +3

Query: 123 VHPDGKAIGVDHVNDLVKTSIDNCKK 200
           V P GK IG+DH+ +LV  SI+N +K
Sbjct: 100 VGPSGKVIGIDHIKELVDDSINNVRK 125
>sp|P22062|PIMT_RAT Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (Protein L-isoaspartyl/D-aspartyl methyltransferase)
           (L-isoaspartyl protein carboxyl methyltransferase)
          Length = 227

 Score =  106 bits (264), Expect = 5e-23
 Identities = 56/93 (60%), Positives = 63/93 (67%)
 Frame = +1

Query: 220 LLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGYN 399
           LL S  ++    DGR GF E APYDAIHVGAAA  +P  LI+QLK GGR I PVG  G N
Sbjct: 130 LLSSGRVRLVVGDGRMGFAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGN 189

Query: 400 QSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQ 498
           Q L Q DK  DGS + K +MGV YVPLTD +KQ
Sbjct: 190 QMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

 Score = 32.0 bits (71), Expect = 1.2
 Identities = 13/22 (59%), Positives = 16/22 (72%)
 Frame = +3

Query: 135 GKAIGVDHVNDLVKTSIDNCKK 200
           GK IG+DH+ +LV  SI N KK
Sbjct: 104 GKVIGIDHIKELVDDSITNVKK 125
>sp|Q92047|PIMT_BRARE Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (Protein L-isoaspartyl/D-aspartyl methyltransferase)
           (L-isoaspartyl protein carboxyl methyltransferase)
          Length = 228

 Score =  105 bits (262), Expect = 8e-23
 Identities = 55/94 (58%), Positives = 63/94 (67%)
 Frame = +1

Query: 217 SLLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGY 396
           SL+ S  ++    DGR GF E APYDAIHVGAAA  +P  L++QLK GGR I PVG  G 
Sbjct: 129 SLITSGRIKLIVGDGRMGFTEEAPYDAIHVGAAAPTVPQALLDQLKPGGRLILPVGPAGG 188

Query: 397 NQSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQ 498
           NQ L Q DK  DGS + K +MGV YVPLTD  KQ
Sbjct: 189 NQMLEQYDKLEDGSTKMKPLMGVIYVPLTDKDKQ 222

 Score = 37.0 bits (84), Expect = 0.036
 Identities = 16/34 (47%), Positives = 22/34 (64%)
 Frame = +3

Query: 123 VHPDGKAIGVDHVNDLVKTSIDNCKKKSRNFFTT 224
           V P GK IG+DH+ +LV+ SI N KK   +  T+
Sbjct: 100 VGPTGKVIGIDHIKELVEDSIANVKKDDPSLITS 133
>sp|P23506|PIMT_MOUSE Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (Protein L-isoaspartyl/D-aspartyl methyltransferase)
           (L-isoaspartyl protein carboxyl methyltransferase)
          Length = 227

 Score =  105 bits (261), Expect = 1e-22
 Identities = 55/93 (59%), Positives = 63/93 (67%)
 Frame = +1

Query: 220 LLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGYN 399
           LL S  ++    DGR G+ E APYDAIHVGAAA  +P  LI+QLK GGR I PVG  G N
Sbjct: 130 LLSSGRVRLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGN 189

Query: 400 QSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQ 498
           Q L Q DK  DGS + K +MGV YVPLTD +KQ
Sbjct: 190 QMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

 Score = 32.0 bits (71), Expect = 1.2
 Identities = 13/22 (59%), Positives = 16/22 (72%)
 Frame = +3

Query: 135 GKAIGVDHVNDLVKTSIDNCKK 200
           GK IG+DH+ +LV  SI N KK
Sbjct: 104 GKVIGIDHIKELVDDSITNVKK 125
>sp|P15246|PIMT_BOVIN Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (Protein L-isoaspartyl/D-aspartyl methyltransferase)
           (L-isoaspartyl protein carboxyl methyltransferase)
          Length = 226

 Score =  104 bits (259), Expect = 2e-22
 Identities = 55/93 (59%), Positives = 62/93 (66%)
 Frame = +1

Query: 220 LLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGYN 399
           LL S  +Q    DGR G+   APYDAIHVGAAA  +P  LI+QLK GGR I PVG  G N
Sbjct: 129 LLSSGRVQLVVGDGRMGYAAEAPYDAIHVGAAAPVVPQALIDQLKPGGRLILPVGPAGGN 188

Query: 400 QSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQ 498
           Q L Q DK  DGS + K +MGV YVPLTD +KQ
Sbjct: 189 QMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 221

 Score = 35.0 bits (79), Expect = 0.14
 Identities = 14/26 (53%), Positives = 19/26 (73%)
 Frame = +3

Query: 123 VHPDGKAIGVDHVNDLVKTSIDNCKK 200
           V P GK IG+DH+ +LV  SI+N +K
Sbjct: 99  VGPSGKVIGIDHIKELVDDSINNVRK 124
>sp|Q43209|PIMT_WHEAT Protein-L-isoaspartate O-methyltransferase (Protein-beta-aspartate
           methyltransferase) (PIMT) (Protein L-isoaspartyl
           methyltransferase) (L-isoaspartyl protein carboxyl
           methyltransferase)
          Length = 230

 Score =  101 bits (251), Expect = 2e-21
 Identities = 52/96 (54%), Positives = 66/96 (68%)
 Frame = +1

Query: 214 SSLLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADG 393
           ++L++  +L FH +DGR G+P+ APYDAIHVGAAA +IP  L+EQLK GGR + PVG   
Sbjct: 134 AALMKDGSLSFHVSDGRLGWPDAAPYDAIHVGAAAPEIPRPLLEQLKPGGRMVIPVGT-- 191

Query: 394 YNQSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQL 501
           Y+Q L  IDK +DGS   +    VRYVPLT    QL
Sbjct: 192 YSQDLQVIDKSADGSTSVRNDASVRYVPLTSRSAQL 227

 Score = 30.8 bits (68), Expect = 2.6
 Identities = 10/28 (35%), Positives = 21/28 (75%)
 Frame = +3

Query: 123 VHPDGKAIGVDHVNDLVKTSIDNCKKKS 206
           V P+G+A+G++H+ +LV  S +N ++ +
Sbjct: 105 VGPEGRAVGIEHIPELVVASTENVERSA 132
>sp|Q27869|PIMT_DROME Protein-L-isoaspartate(D-aspartate) O-methyltransferase
           (Protein-beta-aspartate methyltransferase) (PIMT)
           (dPIMT) (Protein L-isoaspartyl/D-aspartyl
           methyltransferase) (L-isoaspartyl protein carboxyl
           methyltransferase)
          Length = 226

 Score = 99.0 bits (245), Expect = 8e-21
 Identities = 50/92 (54%), Positives = 62/92 (67%)
 Frame = +1

Query: 217 SLLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGY 396
           S+L+S  L     DGR G+P +APY+AIHVGAAA   P +LI QL  GGR I PVG DG 
Sbjct: 134 SMLDSGQLLIVEGDGRKGYPPNAPYNAIHVGAAAPDTPTELINQLASGGRLIVPVGPDGG 193

Query: 397 NQSLVQIDKKSDGSFERKEMMGVRYVPLTDLK 492
           +Q + Q DK ++G  E   +MGV YVPLTDL+
Sbjct: 194 SQYMQQYDKDANGKVEMTRLMGVMYVPLTDLR 225
>sp|Q42539|PIMT_ARATH Protein-L-isoaspartate O-methyltransferase (Protein-beta-aspartate
           methyltransferase) (PIMT) (Protein L-isoaspartyl
           methyltransferase) (L-isoaspartyl protein carboxyl
           methyltransferase)
          Length = 230

 Score = 98.2 bits (243), Expect = 1e-20
 Identities = 52/96 (54%), Positives = 63/96 (65%)
 Frame = +1

Query: 214 SSLLESKALQFHATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADG 393
           S  L+ ++L  H  DGR G+ E APYDAIHVGAAA +IP+ LI+QLK GGR + PVG   
Sbjct: 134 SPFLKERSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKPGGRLVIPVG--N 191

Query: 394 YNQSLVQIDKKSDGSFERKEMMGVRYVPLTDLKKQL 501
             Q L  +DK SDGS   K+   VRYVPLT  + QL
Sbjct: 192 IFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQL 227

 Score = 29.3 bits (64), Expect = 7.6
 Identities = 12/32 (37%), Positives = 22/32 (68%)
 Frame = +3

Query: 123 VHPDGKAIGVDHVNDLVKTSIDNCKKKSRNFF 218
           V  +G+AIGV+H+ +LV +S+ N +  + + F
Sbjct: 105 VGTEGRAIGVEHIPELVASSVKNIEASAASPF 136
>sp|Q6NCU3|PIMT_RHOPA Protein-L-isoaspartate O-methyltransferase (Protein-beta-aspartate
           methyltransferase) (PIMT) (Protein L-isoaspartyl
           methyltransferase) (L-isoaspartyl protein carboxyl
           methyltransferase)
          Length = 218

 Score = 78.6 bits (192), Expect = 1e-14
 Identities = 36/78 (46%), Positives = 51/78 (65%)
 Frame = +1

Query: 247 HATDGRNGFPEHAPYDAIHVGAAADKIPDKLIEQLKVGGRFICPVGADGYNQSLVQIDKK 426
           H +DG  G+P  APYDAI V A   ++P+ L  QLK+GGR + PVGAD   Q LV++ + 
Sbjct: 121 HRSDGTRGWPAAAPYDAIVVAAGGPQVPESLKAQLKIGGRLVMPVGADQQAQELVRLTRL 180

Query: 427 SDGSFERKEMMGVRYVPL 480
            +  F+R+ +  VR+VPL
Sbjct: 181 GEADFKREHLGDVRFVPL 198
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 67,528,186
Number of Sequences: 369166
Number of extensions: 1381954
Number of successful extensions: 3467
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3287
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3448
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4406202450
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)