Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_F21
(528 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P30101|PDIA3_HUMAN Protein disulfide-isomerase A3 precur... 60 4e-09
sp|P38657|PDIA3_BOVIN Protein disulfide-isomerase A3 precur... 60 4e-09
sp|P27773|PDIA3_MOUSE Protein disulfide-isomerase A3 precur... 59 9e-09
sp|P11598|PDIA3_RAT Protein disulfide-isomerase A3 precurso... 56 5e-08
sp|P38659|PDIA4_RAT Protein disulfide-isomerase A4 precurso... 35 0.15
sp|P08003|PDIA4_MOUSE Protein disulfide-isomerase A4 precur... 35 0.15
sp|P13667|PDIA4_HUMAN Protein disulfide-isomerase A4 precur... 33 0.42
sp|P38658|ERP60_SCHMA Probable protein disulfide-isomerase ... 32 0.72
sp|O75882|ATRN_HUMAN Attractin precursor (Mahogany homolog)... 30 2.8
sp|Q9QZW9|HLXB9_MOUSE Homeobox protein HB9 30 2.8
>sp|P30101|PDIA3_HUMAN Protein disulfide-isomerase A3 precursor (Disulfide isomerase
ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57)
(58 kDa glucose regulated protein)
Length = 505
Score = 59.7 bits (143), Expect = 4e-09
Identities = 31/56 (55%), Positives = 38/56 (67%)
Frame = +3
Query: 282 LLVALYSVDYEKNPKGTNYWRNRIMKVAKKFR*FWKICSFLLFQISQEQSHELSEF 449
LL+A Y VDYEKN KG+NYWRNR+M VAKKF +F + + SHELS+F
Sbjct: 260 LLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVAS-RKTFSHELSDF 314
Score = 36.2 bits (82), Expect = 0.050
Identities = 15/55 (27%), Positives = 27/55 (49%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFEESQIKY 168
+ FL A L +++R AH ES ++ +++ P + NKFE+ + Y
Sbjct: 168 HSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAY 222
>sp|P38657|PDIA3_BOVIN Protein disulfide-isomerase A3 precursor (Disulfide isomerase
ER-60) (ERP60) (58 kDa microsomal protein) (P58) (ERp57)
Length = 505
Score = 59.7 bits (143), Expect = 4e-09
Identities = 31/56 (55%), Positives = 38/56 (67%)
Frame = +3
Query: 282 LLVALYSVDYEKNPKGTNYWRNRIMKVAKKFR*FWKICSFLLFQISQEQSHELSEF 449
LL+A Y VDYEKN KG+NYWRNR+M VAKKF + F + + SHELS+F
Sbjct: 260 LLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGQKLHFAVAS-RKTFSHELSDF 314
Score = 35.8 bits (81), Expect = 0.066
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFEESQIKY 168
+ FL A L +++R AH ES +K ++ P + NKFE+ + Y
Sbjct: 168 HSEFLKAASNLRDNYRFAHTNVESLVNKYDDDGEGITLFRPSHLTNKFEDKTVAY 222
>sp|P27773|PDIA3_MOUSE Protein disulfide-isomerase A3 precursor (Disulfide isomerase
ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57)
Length = 504
Score = 58.5 bits (140), Expect = 9e-09
Identities = 31/56 (55%), Positives = 37/56 (66%)
Frame = +3
Query: 282 LLVALYSVDYEKNPKGTNYWRNRIMKVAKKFR*FWKICSFLLFQISQEQSHELSEF 449
LL A Y VDYEKN KG+NYWRNR+M VAKKF +F + + SHELS+F
Sbjct: 259 LLTAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVAS-RKTFSHELSDF 313
Score = 36.2 bits (82), Expect = 0.050
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFEESQIKY 168
+ FL A L +++R AH ES + I+ P +ANKFE+ + Y
Sbjct: 167 HSEFLKAASNLRDNYRFAHTNIESLVKEYDDNGEGITIFRPLHLANKFEDKTVAY 221
>sp|P11598|PDIA3_RAT Protein disulfide-isomerase A3 precursor (Disulfide isomerase
ER-60) (ERp60) (58 kDa microsomal protein) (p58) (ERp57)
(HIP-70) (Q-2)
Length = 505
Score = 56.2 bits (134), Expect = 5e-08
Identities = 30/56 (53%), Positives = 36/56 (64%)
Frame = +3
Query: 282 LLVALYSVDYEKNPKGTNYWRNRIMKVAKKFR*FWKICSFLLFQISQEQSHELSEF 449
LL A Y VDYEKN KG+NYWRNR+M VAK F +F + + SHELS+F
Sbjct: 260 LLTAYYDVDYEKNTKGSNYWRNRVMMVAKTFLDAGHKLNFAVAS-RKTFSHELSDF 314
Score = 35.4 bits (80), Expect = 0.086
Identities = 17/55 (30%), Positives = 26/55 (47%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFEESQIKY 168
+ FL A L +++R AH ES + I+ P +ANKFE+ + Y
Sbjct: 168 HSEFLKAASNLRDNYRFAHTNVESLVKEYDDNGEGITIFRPLHLANKFEDKIVAY 222
>sp|P38659|PDIA4_RAT Protein disulfide-isomerase A4 precursor (Protein ERp-72) (ERp72)
(Calcium-binding protein 2) (CaBP2)
Length = 643
Score = 34.7 bits (78), Expect = 0.15
Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
Frame = +3
Query: 183 RDIKLEHGLK*K*WGMVGLRTSSQHEIFSHPNLLLVALYSVDYEKNPK-GTNYWRNRIMK 359
+D ++H L +VG R +S L+V YSVD+ + + T +WRN++++
Sbjct: 384 KDYVVKHALP-----LVGHRKTSNDAKRYSKRPLVVVYYSVDFSFDYRTATQFWRNKVLE 438
Query: 360 VAKKF 374
VAK F
Sbjct: 439 VAKDF 443
Score = 30.0 bits (66), Expect = 3.6
Identities = 13/49 (26%), Positives = 21/49 (42%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFE 150
Y + A L ED++ H F +K V+ P+K +K+E
Sbjct: 318 YLQYQDAANTLREDYKFHHTFSTEIAKFLKVSLGKLVLMQPEKFQSKYE 366
>sp|P08003|PDIA4_MOUSE Protein disulfide-isomerase A4 precursor (Protein ERp-72) (ERp72)
Length = 638
Score = 34.7 bits (78), Expect = 0.15
Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
Frame = +3
Query: 183 RDIKLEHGLK*K*WGMVGLRTSSQHEIFSHPNLLLVALYSVDYEKNPKG-TNYWRNRIMK 359
+D ++H L +VG R +S L+V YSVD+ + + T +WRN++++
Sbjct: 379 KDYVVKHALP-----LVGHRKTSNDAKRYSKRPLVVVYYSVDFSFDYRAATQFWRNKVLE 433
Query: 360 VAKKF 374
VAK F
Sbjct: 434 VAKDF 438
Score = 33.1 bits (74), Expect = 0.42
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFE 150
Y + A L ED++ H F +K V+ HP+K +K+E
Sbjct: 313 YLQYQDAANNLREDYKFHHTFSPEIAKFLKVSLGKLVLTHPEKFQSKYE 361
>sp|P13667|PDIA4_HUMAN Protein disulfide-isomerase A4 precursor (Protein ERp-72) (ERp72)
Length = 645
Score = 33.1 bits (74), Expect = 0.42
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = +1
Query: 4 YKAFLSVAEKLNEDFRVAHVFDESFESKIKFRKTTFVIYHPQKMANKFE 150
Y+ + A L ED++ H F +K + V+ P+K +K+E
Sbjct: 320 YQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYE 368
Score = 30.8 bits (68), Expect = 2.1
Identities = 13/32 (40%), Positives = 23/32 (71%), Gaps = 1/32 (3%)
Frame = +3
Query: 282 LLVALYSVDYEKNPKG-TNYWRNRIMKVAKKF 374
L+V YSVD+ + + T +WR+++++VAK F
Sbjct: 414 LVVVYYSVDFSFDYRAATQFWRSKVLEVAKDF 445
>sp|P38658|ERP60_SCHMA Probable protein disulfide-isomerase ER-60 precursor (ERP60)
Length = 484
Score = 32.3 bits (72), Expect = 0.72
Identities = 23/76 (30%), Positives = 39/76 (51%), Gaps = 1/76 (1%)
Frame = +3
Query: 225 GMVGLRTSSQHEIFSHPNLLLVALYS-VDYEKNPKGTNYWRNRIMKVAKKFR*FWKICSF 401
G+VG R+ S F + +L V LY+ + P G Y RNR++K K +K F
Sbjct: 230 GLVGYRSPSNTFYFENSDL--VVLYNNQSIDSYPSGVKYLRNRVLKTLKDNPQKFKNLVF 287
Query: 402 LLFQISQEQSHELSEF 449
+ + + S+E+S++
Sbjct: 288 -AYSFADDFSYEISDY 302
>sp|O75882|ATRN_HUMAN Attractin precursor (Mahogany homolog) (DPPT-L)
Length = 1429
Score = 30.4 bits (67), Expect = 2.8
Identities = 18/53 (33%), Positives = 26/53 (49%)
Frame = -1
Query: 162 NLTFFKFISHFLWMIYNKCCFSKFNFAFKRFIEDMSDSEVFI*FFSNA*KCFI 4
N+TFF ++S+F W I K AF + M + F+ FFS CF+
Sbjct: 1250 NITFFVYVSNFTWPI-------KIQIAFSQHSNFMDLVQFFVTFFS----CFL 1291
>sp|Q9QZW9|HLXB9_MOUSE Homeobox protein HB9
Length = 404
Score = 30.4 bits (67), Expect = 2.8
Identities = 21/71 (29%), Positives = 35/71 (49%), Gaps = 8/71 (11%)
Frame = +2
Query: 53 SLMSSMNLL----KAKLNFEKQHLLYIIHK----KWLINLKKVKLNTKAMLQRYQIRTWV 208
S + NLL + + F Q LL + H+ K+L K+ ++ T ML Q++ W
Sbjct: 230 SCQAQSNLLGKCRRPRTAFTSQQLLELEHQFKLNKYLSRPKRFEVATSLMLTETQVKIWF 289
Query: 209 EVKIMGYGRSK 241
+ + M + RSK
Sbjct: 290 QNRRMKWKRSK 300
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,517,417
Number of Sequences: 369166
Number of extensions: 1068871
Number of successful extensions: 2432
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2379
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2432
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3551563800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)