Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_E10
(699 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99PM3|TF2AA_MOUSE Transcription initiation factor IIA s... 93 6e-19
sp|P52655|TF2AA_HUMAN Transcription initiation factor IIA s... 93 6e-19
sp|Q5RCU0|TF2AA_PONPY Transcription initiation factor IIA s... 93 6e-19
sp|O08949|TF2AA_RAT Transcription initiation factor IIA sub... 93 6e-19
sp|P52654|TF2AA_DROME Transcription initiation factor IIA s... 92 1e-18
sp|Q9UNN4|TF2AY_HUMAN TFIIA-alpha and beta-like factor (Gen... 82 1e-15
sp|Q8R4I4|TF2AY_MOUSE TFIIA-alpha and beta-like factor (Gen... 81 3e-15
sp|P32773|TOA1_YEAST Transcription initiation factor IIA la... 51 3e-06
sp|Q8BIE6|FRM4A_MOUSE FERM domain containing protein 4A 33 0.56
sp|Q9P2Q2|FRM4A_HUMAN FERM domain containing protein 4A 33 0.56
>sp|Q99PM3|TF2AA_MOUSE Transcription initiation factor IIA subunit 1 (General
transcription factor IIA1) [Contains: Transcription
initiation factor IIA alpha chain (TFIIA p35 subunit);
Transcription initiation factor IIA beta chain (TFIIA
p19 subunit)]
Length = 378
Score = 93.2 bits (230), Expect = 6e-19
Identities = 35/64 (54%), Positives = 54/64 (84%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK + GI NL G+D +F KA+G
Sbjct: 315 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 374
Query: 592 EAEW 603
+AEW
Sbjct: 375 DAEW 378
>sp|P52655|TF2AA_HUMAN Transcription initiation factor IIA subunit 1 (General
transcription factor IIA1) (TFIIA-42) (TFIIAL)
[Contains: Transcription initiation factor IIA alpha
chain (TFIIA p35 subunit); Transcription initiation
factor IIA beta chain (TFIIA p19 subunit)]
Length = 376
Score = 93.2 bits (230), Expect = 6e-19
Identities = 35/64 (54%), Positives = 54/64 (84%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK + GI NL G+D +F KA+G
Sbjct: 313 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 372
Query: 592 EAEW 603
+AEW
Sbjct: 373 DAEW 376
>sp|Q5RCU0|TF2AA_PONPY Transcription initiation factor IIA subunit 1 (General
transcription factor IIA1) [Contains: Transcription
initiation factor IIA alpha chain (TFIIA p35 subunit);
Transcription initiation factor IIA beta chain (TFIIA
p19 subunit)]
Length = 376
Score = 93.2 bits (230), Expect = 6e-19
Identities = 35/64 (54%), Positives = 54/64 (84%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK + GI NL G+D +F KA+G
Sbjct: 313 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 372
Query: 592 EAEW 603
+AEW
Sbjct: 373 DAEW 376
>sp|O08949|TF2AA_RAT Transcription initiation factor IIA subunit 1 (General
transcription factor IIA1) [Contains: Transcription
initiation factor IIA alpha chain (TFIIA p35 subunit);
Transcription initiation factor IIA beta chain (TFIIA
p19 subunit)]
Length = 377
Score = 93.2 bits (230), Expect = 6e-19
Identities = 35/64 (54%), Positives = 54/64 (84%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNSEDD+SD++ +++F+ +NV+VCQY+K++R++NKWK + GI NL G+D +F KA+G
Sbjct: 314 PLNSEDDVSDEEGQELFDTENVVVCQYDKIHRSKNKWKFHLKDGIMNLNGRDYIFSKAIG 373
Query: 592 EAEW 603
+AEW
Sbjct: 374 DAEW 377
>sp|P52654|TF2AA_DROME Transcription initiation factor IIA subunit 1 (dTFIIA-L) [Contains:
Transcription initiation factor IIA alpha chain (TFIIA
p30 subunit); Transcription initiation factor IIA beta
chain (TFIIA p20 subunit)]
Length = 366
Score = 92.0 bits (227), Expect = 1e-18
Identities = 38/64 (59%), Positives = 51/64 (79%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNSEDD++D+D ++F+ DNVIVCQY+K+ R+RNKWK GI N++GKD VF K+ G
Sbjct: 303 PLNSEDDVTDEDSAEMFDTDNVIVCQYDKITRSRNKWKFYLKDGIMNMRGKDYVFQKSNG 362
Query: 592 EAEW 603
+AEW
Sbjct: 363 DAEW 366
>sp|Q9UNN4|TF2AY_HUMAN TFIIA-alpha and beta-like factor (General transcription factor II
A, 1-like factor)
Length = 478
Score = 82.4 bits (202), Expect = 1e-15
Identities = 34/64 (53%), Positives = 47/64 (73%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNS DD+S+ D +F+ DNVIVCQY+K++R++NKWK G+ G+D VF KA+G
Sbjct: 415 PLNSGDDVSEQDVPDLFDTDNVIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIG 474
Query: 592 EAEW 603
+AEW
Sbjct: 475 DAEW 478
>sp|Q8R4I4|TF2AY_MOUSE TFIIA-alpha and beta-like factor (General transcription factor II
A, 1-like factor)
Length = 468
Score = 80.9 bits (198), Expect = 3e-15
Identities = 33/64 (51%), Positives = 47/64 (73%)
Frame = +1
Query: 412 PLNSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVG 591
PLNS DD+S+ D +F+ +NVIVCQY+K++R++N+WK G+ G+D VF KA+G
Sbjct: 405 PLNSGDDVSEQDVPDLFDTENVIVCQYDKIHRSKNRWKFYLKDGVMCFGGRDYVFAKAIG 464
Query: 592 EAEW 603
EAEW
Sbjct: 465 EAEW 468
>sp|P32773|TOA1_YEAST Transcription initiation factor IIA large chain (TFIIA 32 kDa
subunit)
Length = 286
Score = 50.8 bits (120), Expect = 3e-06
Identities = 22/62 (35%), Positives = 36/62 (58%)
Frame = +1
Query: 418 NSEDDISDDDPEKIFEADNVIVCQYEKVNRTRNKWKLNFVSGIANLQGKDSVFHKAVGEA 597
+S+DD + E+ +N+++C Y+KV RT+ +WK + G+ + D F KA EA
Sbjct: 224 DSDDDYLISEGEEDGPDENLMLCLYDKVTRTKARWKCSLKDGVVTINRNDYTFQKAQVEA 283
Query: 598 EW 603
EW
Sbjct: 284 EW 285
>sp|Q8BIE6|FRM4A_MOUSE FERM domain containing protein 4A
Length = 1020
Score = 33.5 bits (75), Expect = 0.56
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +2
Query: 452 RKYSRQTTLSFASMRRSTELATSGSSISLVESRICRARIQCSTRRSARPSGERAVTPS 625
+++SR + S S ++ +TS S + SR+ R C +A P +R+ TPS
Sbjct: 921 KEHSRLSHTSSTSSDSGSQYSTSSQSTFVAHSRVTRMPQMCKATSAALPQSQRSSTPS 978
>sp|Q9P2Q2|FRM4A_HUMAN FERM domain containing protein 4A
Length = 1024
Score = 33.5 bits (75), Expect = 0.56
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +2
Query: 452 RKYSRQTTLSFASMRRSTELATSGSSISLVESRICRARIQCSTRRSARPSGERAVTPS 625
+++SR + S S ++ +TS S + SR+ R C +A P +R+ TPS
Sbjct: 925 KEHSRLSHTSSTSSDSGSQYSTSSQSTFVAHSRVTRMPQMCKATSAALPQSQRSSTPS 982
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,742,765
Number of Sequences: 369166
Number of extensions: 822498
Number of successful extensions: 2196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2196
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6073541875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)