Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_E07
(597 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochon... 172 6e-43
sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondr... 167 1e-41
sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochon... 159 7e-39
sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochon... 159 7e-39
sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Perox... 136 3e-32
sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Perox... 136 5e-32
sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase... 134 2e-31
sp|Q8S4Y1|THIC1_ARATH Acetyl-CoA acetyltransferase, cytosol... 130 3e-30
sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxiso... 130 3e-30
sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoace... 127 2e-29
>sp|Q8QZT1|THIL_MOUSE Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 172 bits (436), Expect = 6e-43
Identities = 85/175 (48%), Positives = 129/175 (73%), Gaps = 1/175 (0%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
EDEE R +F + LK F K +GTIT L+L + +A + ++KP+A+
Sbjct: 249 EDEEYKRVDFSKVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAEAAQRLNVKPLAR 308
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDIN 359
+ F+DAA++P +F +AP+ ++ K+L+ L+ +DI++WE+NEAFSV VLAN+K++ +I+
Sbjct: 309 IAAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKML-EID 367
Query: 360 IENVNIHGGAVSLGHPLGMSGARIVLHLALNLQPGQKGIATVCNGGGGASSILLE 524
+ VNIHGGAVSLGHP+GMSGARIV+H+A L+PG+ G+A++CNGGGGAS++L+E
Sbjct: 368 PQKVNIHGGAVSLGHPIGMSGARIVVHMAHALKPGEFGLASICNGGGGASALLIE 422
>sp|P17764|THIL_RAT Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 424
Score = 167 bits (424), Expect = 1e-41
Identities = 82/175 (46%), Positives = 127/175 (72%), Gaps = 1/175 (0%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
EDEE R +F + LK F K +GT+T ++L + +A + +KP+A+
Sbjct: 249 EDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAAVVLMTAEAAQRLKVKPLAR 308
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDIN 359
+ F+DAA++P +F +AP+ ++ K+L+ L+ +DI++WE+NEAFSV VLAN+K++ +I+
Sbjct: 309 IAAFADAAVDPIDFPLAPAYAVPKVLKYAGLKKEDIAMWEVNEAFSVVVLANIKML-EID 367
Query: 360 IENVNIHGGAVSLGHPLGMSGARIVLHLALNLQPGQKGIATVCNGGGGASSILLE 524
+ VN+HGGAVSLGHP+GMSGARIV+HLA L+ G+ G+A++CNGGGGAS++L+E
Sbjct: 368 PQKVNVHGGAVSLGHPIGMSGARIVVHLAHALKQGEFGLASICNGGGGASAVLIE 422
>sp|P24752|THIL_HUMAN Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase) (T2)
Length = 427
Score = 159 bits (401), Expect = 7e-39
Identities = 81/175 (46%), Positives = 124/175 (70%), Gaps = 1/175 (0%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
EDEE R +F + LK F K +GT+T L+L + DA + ++ P+A+
Sbjct: 252 EDEEYKRVDFSKVPKLKTVFQKENGTVTAANASTLNDGAAALVLMTADAAKRLNVTPLAR 311
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDIN 359
++ F+DAA+EP +F IAP + + +L+ L+ +DI++WE+NEAFS+ VLAN+K++ +I+
Sbjct: 312 IVAFADAAVEPIDFPIAPVYAASMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKML-EID 370
Query: 360 IENVNIHGGAVSLGHPLGMSGARIVLHLALNLQPGQKGIATVCNGGGGASSILLE 524
+ VNI+GGAVSLGHP+GMSGARIV HL L+ G+ G+A++CNGGGGAS++L++
Sbjct: 371 PQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425
>sp|Q8HXY6|THIL_MACFA Acetyl-CoA acetyltransferase, mitochondrial precursor
(Acetoacetyl-CoA thiolase)
Length = 427
Score = 159 bits (401), Expect = 7e-39
Identities = 81/175 (46%), Positives = 124/175 (70%), Gaps = 1/175 (0%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
EDEE R +F + LK F K +GTIT L+L + A + ++ P+A+
Sbjct: 252 EDEEYKRVDFSKVPKLKTVFQKENGTITAANASTLNDGAAALVLMTAGAAKRLNVTPLAR 311
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDIN 359
++ F+DAA+EP +F IAP +++ +L+ L+ +DI++WE+NEAFS+ VLAN+K++ +I+
Sbjct: 312 IVAFADAAVEPIDFPIAPVHAVSMVLKDVGLKKEDIAMWEVNEAFSLVVLANIKML-EID 370
Query: 360 IENVNIHGGAVSLGHPLGMSGARIVLHLALNLQPGQKGIATVCNGGGGASSILLE 524
+ VNI+GGAVSLGHP+GMSGARIV HL L+ G+ G+A++CNGGGGAS++L++
Sbjct: 371 PQKVNINGGAVSLGHPIGMSGARIVGHLTHALKQGEYGLASICNGGGGASAMLIQ 425
>sp|Q12598|THIA_CANTR Acetyl-CoA acetyltransferase IA (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IA)
Length = 403
Score = 136 bits (343), Expect = 3e-32
Identities = 73/176 (41%), Positives = 114/176 (64%), Gaps = 3/176 (1%)
Frame = +3
Query: 6 DEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAKV 182
DEE G+ N +R S + F K +GT+T L+L S+ ++ LKP+AK+
Sbjct: 221 DEEIGKFNEERLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKI 280
Query: 183 LGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDINI 362
G+ +AA P +F IAP+ ++ K ++ L + + +E+NEAFSV LAN +L+ +I +
Sbjct: 281 SGWGEAARTPFDFTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELV-NIPL 339
Query: 363 ENVNIHGGAVSLGHPLGMSGARIVLHL--ALNLQPGQKGIATVCNGGGGASSILLE 524
E +N++GGAV++GHPLG SGARI++ L L + G+ G+A VCNGGGGAS++++E
Sbjct: 340 EKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGVAGVCNGGGGASAVVIE 395
>sp|Q04677|THIB_CANTR Acetyl-CoA acetyltransferase IB (Peroxisomal acetoacetyl-CoA
thiolase) (Thiolase IB)
Length = 403
Score = 136 bits (342), Expect = 5e-32
Identities = 75/176 (42%), Positives = 112/176 (63%), Gaps = 3/176 (1%)
Frame = +3
Query: 6 DEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAKV 182
DEE G+ N DR S + F K +GT+T L+L S+ ++ LKP+AK+
Sbjct: 221 DEEIGKFNEDRLKSARTVFQKENGTVTAPNASKLNDGGAALVLVSEAKLKQLGLKPLAKI 280
Query: 183 LGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDINI 362
G+ +AA P +F IAP+ ++ K ++ L + + +E+NEAFSV LAN +L+ I +
Sbjct: 281 SGWGEAARTPFDFTIAPALAVPKAVKHAGLTVDRVDFFELNEAFSVVGLANAELV-KIPL 339
Query: 363 ENVNIHGGAVSLGHPLGMSGARIVLHL--ALNLQPGQKGIATVCNGGGGASSILLE 524
E +N++GGAV++GHPLG SGARI++ L L + G+ G A VCNGGGGAS+I++E
Sbjct: 340 EKLNVYGGAVAMGHPLGCSGARIIVTLLSVLTQEGGRFGAAGVCNGGGGASAIVIE 395
>sp|Q9FIK7|THIC2_ARATH Probable acetyl-CoA acetyltransferase, cytosolic 2 (Cytosolic
acetoacetyl-CoA thiolase 2) (Thiolase 2)
Length = 415
Score = 134 bits (337), Expect = 2e-31
Identities = 75/177 (42%), Positives = 109/177 (61%), Gaps = 3/177 (1%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAFKSDG-TITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
+DE G+ + + L+P+FK DG ++T L+L S + L IAK
Sbjct: 228 KDEGLGKFDAAKLKKLRPSFKEDGGSVTAGNASSISDGAAALVLVSGEKALELGLHVIAK 287
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDIN 359
+ G++DAA PE F P+ +I K +++ L + +EINEAFSV LAN KL+ ++
Sbjct: 288 IRGYADAAQAPELFTTTPALAIPKAIKRAGLDASQVDYYEINEAFSVVALANQKLL-GLD 346
Query: 360 IENVNIHGGAVSLGHPLGMSGARIVLHL--ALNLQPGQKGIATVCNGGGGASSILLE 524
E +N HGGAVSLGHPLG SGARI++ L L + G+ G+A++CNGGGGAS+++LE
Sbjct: 347 PERLNAHGGAVSLGHPLGCSGARILVTLLGVLRAKKGKYGVASICNGGGGASALVLE 403
>sp|Q8S4Y1|THIC1_ARATH Acetyl-CoA acetyltransferase, cytosolic 1 (Cytosolic
acetoacetyl-CoA thiolase 1) (Thiolase 1)
Length = 403
Score = 130 bits (326), Expect = 3e-30
Identities = 76/177 (42%), Positives = 107/177 (60%), Gaps = 3/177 (1%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAFKSDG-TITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
+DE G+ + + L+P+FK +G T+T L+L S + L +AK
Sbjct: 226 KDEGLGKFDAAKLRKLRPSFKENGGTVTAGNASSISDGAAALVLVSGEKALQLGLLVLAK 285
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDIN 359
+ G+ DAA EPE F AP+ +I K + L+ + +EINEAF+V LAN KL+ I
Sbjct: 286 IKGYGDAAQEPEFFTTAPALAIPKAIAHAGLESSQVDYYEINEAFAVVALANQKLL-GIA 344
Query: 360 IENVNIHGGAVSLGHPLGMSGARIVLHL--ALNLQPGQKGIATVCNGGGGASSILLE 524
E VN++GGAVSLGHPLG SGARI++ L L + G+ G+ VCNGGGGAS+++LE
Sbjct: 345 PEKVNVNGGAVSLGHPLGCSGARILITLLGILKKRNGKYGVGGVCNGGGGASALVLE 401
>sp|Q6L8K7|THIL_YARLI Acetyl-CoA acetyltransferase (Peroxisomal acetoacetyl-CoA thiolase)
(Thiolase)
Length = 397
Score = 130 bits (326), Expect = 3e-30
Identities = 71/174 (40%), Positives = 104/174 (59%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAFKSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAKV 182
EDEE AN ++ LKP F G++T +++AS + +AK+
Sbjct: 219 EDEEPKGANAEKLKGLKPVFDKQGSVTAGNASPINDGASAVVVASGTKAKELGTPVLAKI 278
Query: 183 LGFSDAAMEPENFVIAPSESINKLLQKHALQIKDISLWEINEAFSVAVLANLKLIPDINI 362
+ ++DAA P +F IAPS +I L+K L DI+LWEINEAFS LANL + I+
Sbjct: 279 VSYADAATAPIDFTIAPSLAIPAALKKAGLTKDDIALWEINEAFSGVALANLMRL-GIDK 337
Query: 363 ENVNIHGGAVSLGHPLGMSGARIVLHLALNLQPGQKGIATVCNGGGGASSILLE 524
VN+ GGAV+LGHP+G SG RI + L L+ G+ G+A +CNGGG +++I+++
Sbjct: 338 SKVNVKGGAVALGHPIGASGNRIFVTLVNALKEGEYGVAAICNGGGASTAIVIK 391
>sp|P41338|THIL_YEAST Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)
Length = 398
Score = 127 bits (320), Expect = 2e-29
Identities = 71/178 (39%), Positives = 111/178 (62%), Gaps = 4/178 (2%)
Frame = +3
Query: 3 EDEECGRANFDRFASLKPAF-KSDGTITXXXXXXXXXXXXCLLLASKDAIESNDLKPIAK 179
+DEE R + ++ S + F K +GT+T ++L S+ ++ +LKP+A
Sbjct: 220 KDEEPARLHVEKLRSARTVFQKENGTVTAANASPINDGAAAVILVSEKVLKEKNLKPLAI 279
Query: 180 VLGFSDAAMEPENFVIAPSESINKLLQKHALQ-IKDISLWEINEAFSVAVLANLKLIPDI 356
+ G+ +AA +P +F APS ++ K L+ ++ I + +E NEAFSV L N K++ +
Sbjct: 280 IKGWGEAAHQPADFTWAPSLAVPKALKHAGIEDINSVDYFEFNEAFSVVGLVNTKILK-L 338
Query: 357 NIENVNIHGGAVSLGHPLGMSGARIVLHLALNLQP--GQKGIATVCNGGGGASSILLE 524
+ VN++GGAV+LGHPLG SGAR+V+ L LQ G+ G+A +CNGGGGASSI++E
Sbjct: 339 DPSKVNVYGGAVALGHPLGCSGARVVVTLLSILQQEGGKIGVAAICNGGGGASSIVIE 396
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,489,987
Number of Sequences: 369166
Number of extensions: 1386666
Number of successful extensions: 4541
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4294
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4444
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4553075865
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)