Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_C10
(914 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1 61 5e-09
sp|Q90XC2|NEK8_BRARE Serine/threonine-protein kinase Nek8 (... 60 9e-09
sp|Q02595|KPK2_PLAFK Probable serine/threonine-protein kina... 60 1e-08
sp|P18106|FPS_DROME Tyrosine-protein kinase Fps85D (dFer) 60 1e-08
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 59 1e-08
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 59 1e-08
sp|P41892|CDC7_SCHPO Cell division control protein 7 59 2e-08
sp|Q86SG6|NEK8_HUMAN Serine/threonine-protein kinase Nek8 (... 59 2e-08
sp|P51954|NEK1_MOUSE Serine/threonine-protein kinase Nek1 (... 59 2e-08
sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/S... 59 2e-08
>sp|Q10407|MKH1_SCHPO MAP kinase kinase kinase mkh1
Length = 1116
Score = 60.8 bits (146), Expect = 5e-09
Identities = 36/105 (34%), Positives = 58/105 (55%), Gaps = 6/105 (5%)
Frame = +3
Query: 618 VKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKL------SFERELQVYS 779
+K E +G G++G VF ++I T + VK+++IP T + D+L S E+ + +
Sbjct: 826 MKGELIGNGTYGKVFLAMNINTGELIAVKQVEIPQTINGRHDQLRKDIVDSINAEISMIA 885
Query: 780 KHSDHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRKNGPLSE 914
DH IV+Y KTE + + +EY+ GGS+G +R GP E
Sbjct: 886 D-LDHLNIVQYLGFEKTETDISIFLEYVSGGSIGRCLRNYGPFEE 929
>sp|Q90XC2|NEK8_BRARE Serine/threonine-protein kinase Nek8 (NimA-related protein kinase
8)
Length = 697
Score = 60.1 bits (144), Expect = 9e-09
Identities = 34/96 (35%), Positives = 53/96 (55%)
Frame = +3
Query: 609 ENLVKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFERELQVYSKHS 788
E K + +G G+FG V C T +V+ K +IP ++++L+ + E QV K
Sbjct: 2 EKYEKTKVVGRGAFGIVHLCRR-RTDSALVILK-EIPVEQMTRDERLAAQNECQVL-KLL 58
Query: 789 DHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRK 896
HP I++Y NF + A+ + MEY GG+L YI+K
Sbjct: 59 SHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQK 94
>sp|Q02595|KPK2_PLAFK Probable serine/threonine-protein kinase 2
Length = 510
Score = 59.7 bits (143), Expect = 1e-08
Identities = 40/120 (33%), Positives = 67/120 (55%), Gaps = 5/120 (4%)
Frame = +3
Query: 570 DDLQLYFNVEP----DFENLVKL-EKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKA 734
D++ +FN+ F+N+ L +K+G+GSF T + +I +VVK++ DK+
Sbjct: 91 DEMSQFFNMLGYPILGFKNVYVLNKKIGKGSFSTAYIGTNILYGNRVVVKEV-----DKS 145
Query: 735 KEDKLSFERELQVYSKHSDHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRKNGPLSE 914
K + + E++V K H I+K ++ E V+LV+EY+KGG L Y+ NGP +E
Sbjct: 146 KVKESNVYTEIEVLRKVM-HKYIIKLISAYEQEGFVYLVLEYLKGGELFEYLNNNGPYTE 204
>sp|P18106|FPS_DROME Tyrosine-protein kinase Fps85D (dFer)
Length = 1325
Score = 59.7 bits (143), Expect = 1e-08
Identities = 36/97 (37%), Positives = 54/97 (55%)
Frame = +3
Query: 609 ENLVKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFERELQVYSKHS 788
+++V LE++G G+FG V+K TK ++ VK ++ D+ K L R L+ Y
Sbjct: 1061 DDVVLLERIGRGNFGDVYKAKLKSTKLDVAVKTCRMTLPDEQKRKFLQEGRILKQY---- 1116
Query: 789 DHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRKN 899
DHP IVK + + +VME + GGSL Y+RKN
Sbjct: 1117 DHPNIVKLIGICVQKQPIMIVMELVLGGSLLTYLRKN 1153
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 59.3 bits (142), Expect = 1e-08
Identities = 39/109 (35%), Positives = 67/109 (61%), Gaps = 1/109 (0%)
Frame = +3
Query: 591 NVEPDFENL-VKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFEREL 767
N++ D E L KLEK+G+GSFG VFK I+ + + VV +KI ++A+++ ++E+
Sbjct: 27 NLKADPEELFTKLEKIGKGSFGEVFK--GIDNRTQKVV-AIKIIDLEEAEDEIEDIQQEI 83
Query: 768 QVYSKHSDHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRKNGPLSE 914
V S+ D P + KY ++ + ++++MEY+ GGS + + GPL E
Sbjct: 84 TVLSQ-CDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLL-EPGPLDE 130
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 59.3 bits (142), Expect = 1e-08
Identities = 39/109 (35%), Positives = 67/109 (61%), Gaps = 1/109 (0%)
Frame = +3
Query: 591 NVEPDFENL-VKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFEREL 767
N++ D E L KLEK+G+GSFG VFK I+ + + VV +KI ++A+++ ++E+
Sbjct: 15 NLKADPEELFTKLEKIGKGSFGEVFK--GIDNRTQKVV-AIKIIDLEEAEDEIEDIQQEI 71
Query: 768 QVYSKHSDHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRKNGPLSE 914
V S+ D P + KY ++ + ++++MEY+ GGS + + GPL E
Sbjct: 72 TVLSQ-CDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLL-EPGPLDE 118
>sp|P41892|CDC7_SCHPO Cell division control protein 7
Length = 1062
Score = 58.9 bits (141), Expect = 2e-08
Identities = 30/94 (31%), Positives = 56/94 (59%)
Frame = +3
Query: 633 LGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFERELQVYSKHSDHPRIVKY 812
LG+G+FG V++ ++I+ + VKK+K+ K+ + E +L K+ DHP IVKY
Sbjct: 15 LGKGAFGAVYRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLL---KNLDHPNIVKY 71
Query: 813 ERNFKTENAVFLVMEYMKGGSLGGYIRKNGPLSE 914
+++T +++ +++EY + GSL + G + E
Sbjct: 72 RGSYQTNDSLCIILEYCENGSLRSICKNFGKIPE 105
>sp|Q86SG6|NEK8_HUMAN Serine/threonine-protein kinase Nek8 (NimA-related protein kinase
8) (NIMA-related kinase 12a)
Length = 692
Score = 58.9 bits (141), Expect = 2e-08
Identities = 30/96 (31%), Positives = 54/96 (56%)
Frame = +3
Query: 609 ENLVKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFERELQVYSKHS 788
E ++ +G G+FG V C+ + +++K+ IP KE++ + + E QV K
Sbjct: 2 EKYERIRVVGRGAFGIVHLCLRKADQKLVIIKQ--IPVEQMTKEERQAAQNECQVL-KLL 58
Query: 789 DHPRIVKYERNFKTENAVFLVMEYMKGGSLGGYIRK 896
+HP +++Y NF + A+ + MEY GG+L +I+K
Sbjct: 59 NHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQK 94
>sp|P51954|NEK1_MOUSE Serine/threonine-protein kinase Nek1 (NimA-related protein kinase
1)
Length = 774
Score = 58.5 bits (140), Expect = 2e-08
Identities = 32/91 (35%), Positives = 55/91 (60%), Gaps = 1/91 (1%)
Frame = +3
Query: 609 ENLVKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPT-TDKAKEDKLSFERELQVYSKH 785
E V+L+K+GEGSFG S E V+K++ I +DK +++ RE+ V + +
Sbjct: 2 EKYVRLQKIGEGSFGKAVLVKSTEDGRHYVIKEINISRMSDKERQES---RREVAVLA-N 57
Query: 786 SDHPRIVKYERNFKTENAVFLVMEYMKGGSL 878
HP IV+Y+ +F+ ++++VM+Y +GG L
Sbjct: 58 MKHPNIVQYKESFEENGSLYIVMDYCEGGDL 88
>sp|Q01389|BCK1_YEAST Serine/threonine-protein kinase BCK1/SLK1/SSP31
Length = 1478
Score = 58.5 bits (140), Expect = 2e-08
Identities = 37/103 (35%), Positives = 57/103 (55%), Gaps = 4/103 (3%)
Frame = +3
Query: 618 VKLEKLGEGSFGTVFKCVSIETKGEMVVKKLKIPTTDKAKEDKLSFERELQ---VYSKHS 788
+K E +G+GSFG V+ C+++ T M VK++++P E LS L+ K
Sbjct: 1176 MKGEMIGKGSFGAVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKDL 1235
Query: 789 DHPRIVKYERNFKTENAVF-LVMEYMKGGSLGGYIRKNGPLSE 914
DH IV+Y F+ +N ++ L +EY+ GGS+G IR G E
Sbjct: 1236 DHLNIVQY-LGFENKNNIYSLFLEYVAGGSVGSLIRMYGRFDE 1277
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 98,821,738
Number of Sequences: 369166
Number of extensions: 2057076
Number of successful extensions: 6994
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6383
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6609
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9318621220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)