Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_C06
(903 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q28735|TMEDA_RABIT Transmembrane emp24 domain containing... 248 1e-65
sp|Q63584|TMEDA_RAT Transmembrane emp24 domain containing p... 248 2e-65
sp|Q5E971|TMEDA_BOVIN Transmembrane emp24 domain containing... 248 2e-65
sp|O35587|TMEDA_MESAU Transmembrane protein Tmp21 precursor... 248 2e-65
sp|Q9D1D4|TMEDA_MOUSE Transmembrane emp24 domain containing... 247 3e-65
sp|P49755|TMEDA_HUMAN Transmembrane emp24 domain containing... 247 4e-65
sp|Q5RE32|TMEDA_PONPY Transmembrane emp24 domain containing... 246 6e-65
sp|Q90515|TMEDA_FUGRU Transmembrane emp24 domain containing... 241 2e-63
sp|P54837|ERV25_YEAST Protein ERV25 precursor 134 5e-31
sp|Q9BVK6|TMED9_HUMAN Transmembrane emp24 domain containing... 98 4e-20
>sp|Q28735|TMEDA_RABIT Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (21 kDa transmembrane
trafficking protein) (Integral membrane protein p23)
Length = 219
Score = 248 bits (634), Expect = 1e-65
Identities = 124/206 (60%), Positives = 159/206 (77%), Gaps = 5/206 (2%)
Frame = +3
Query: 21 ISLFILNFLYVTSAIRF-FVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKASIQ 185
++L L L +S + F P +KCL+EEIHK++LVTG Y+ITD A G + ++
Sbjct: 16 LALLFLFLLGPSSVLAISFHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTHLK 75
Query: 186 VTDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVETK 365
+TDS H+LY K+D +KGKFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE K
Sbjct: 76 ITDSAGHILYSKEDASKGKFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVEAK 133
Query: 366 NYDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSLFS 545
NY+ +A+ EKLKPLEVEL+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS+FS
Sbjct: 134 NYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFS 193
Query: 546 MACLMGLAIWQVLYLRRYFKSKKLIE 623
M CL+GLA WQV YLRR+FK+KKLIE
Sbjct: 194 MFCLIGLATWQVFYLRRFFKAKKLIE 219
>sp|Q63584|TMEDA_RAT Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (21 kDa transmembrane
trafficking protein)
Length = 219
Score = 248 bits (633), Expect = 2e-65
Identities = 124/208 (59%), Positives = 159/208 (76%), Gaps = 6/208 (2%)
Frame = +3
Query: 18 IISLFILNFLYVTSAIR--FFVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKAS 179
++S + FL S++ F P +KCL+EEIHK++LVTG Y+ITD A G +
Sbjct: 14 LLSALLFLFLLGPSSVLGISFHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTH 73
Query: 180 IQVTDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVE 359
+++TDS H+LY K+D KGKFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE
Sbjct: 74 LKITDSAGHILYAKEDATKGKFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVE 131
Query: 360 TKNYDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSL 539
KNY+ +A+ EKLKPLEVEL+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS+
Sbjct: 132 AKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSI 191
Query: 540 FSMACLMGLAIWQVLYLRRYFKSKKLIE 623
FSM CL+GLA WQV YLRR+FK+KKLIE
Sbjct: 192 FSMFCLIGLATWQVFYLRRFFKAKKLIE 219
>sp|Q5E971|TMEDA_BOVIN Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (21 kDa transmembrane
trafficking protein)
Length = 219
Score = 248 bits (633), Expect = 2e-65
Identities = 124/205 (60%), Positives = 158/205 (77%), Gaps = 5/205 (2%)
Frame = +3
Query: 24 SLFILNFLYVTSAIRF-FVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKASIQV 188
+L +L L +S + F P +KCL+EEIHK++LVTG Y+ITD A G + +++
Sbjct: 17 ALLLLLLLGPSSVLAISFHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTHLKI 76
Query: 189 TDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVETKN 368
TDS H+LY K+D KGKFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE KN
Sbjct: 77 TDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVEAKN 134
Query: 369 YDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSLFSM 548
Y+ +A+ EKLKPLEVEL+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS+FSM
Sbjct: 135 YEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSM 194
Query: 549 ACLMGLAIWQVLYLRRYFKSKKLIE 623
CL+GLA WQV YLRR+FK+KKLIE
Sbjct: 195 FCLIGLATWQVFYLRRFFKAKKLIE 219
>sp|O35587|TMEDA_MESAU Transmembrane protein Tmp21 precursor (21 kDa transmembrane
trafficking protein) (Integral membrane protein p23)
Length = 219
Score = 248 bits (632), Expect = 2e-65
Identities = 120/188 (63%), Positives = 150/188 (79%), Gaps = 4/188 (2%)
Frame = +3
Query: 72 FVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKASIQVTDSKSHVLYQKDDIAKG 239
F P +KCL+EEIHK++LVTG Y+ITD A G + +++TDS H+LY K+D KG
Sbjct: 34 FHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTHLKITDSAGHILYAKEDATKG 93
Query: 240 KFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVETKNYDSLAQAEKLKPLEVEL 419
KFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE KNY+ +A+ EKLKPLEVEL
Sbjct: 94 KFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVEL 151
Query: 420 KRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSLFSMACLMGLAIWQVLYLRRY 599
+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS+FSM CL+GLA WQV YLRR+
Sbjct: 152 RRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMLCLIGLATWQVFYLRRF 211
Query: 600 FKSKKLIE 623
FK+KKLIE
Sbjct: 212 FKAKKLIE 219
>sp|Q9D1D4|TMEDA_MOUSE Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (21 kDa transmembrane
trafficking protein)
Length = 219
Score = 247 bits (631), Expect = 3e-65
Identities = 120/188 (63%), Positives = 150/188 (79%), Gaps = 4/188 (2%)
Frame = +3
Query: 72 FVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKASIQVTDSKSHVLYQKDDIAKG 239
F P +KCL+EEIHK++LVTG Y+ITD A G + +++TDS H+LY K+D KG
Sbjct: 34 FHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTHLKITDSAGHILYAKEDATKG 93
Query: 240 KFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVETKNYDSLAQAEKLKPLEVEL 419
KFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE KNY+ +A+ EKLKPLEVEL
Sbjct: 94 KFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVEL 151
Query: 420 KRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSLFSMACLMGLAIWQVLYLRRY 599
+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS+FSM CL+GLA WQV YLRR+
Sbjct: 152 RRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRF 211
Query: 600 FKSKKLIE 623
FK+KKLIE
Sbjct: 212 FKAKKLIE 219
>sp|P49755|TMEDA_HUMAN Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (21 kDa transmembrane
trafficking protein) (p24delta) (S31III125) (S31I125)
(Tmp-21-I)
Length = 219
Score = 247 bits (630), Expect = 4e-65
Identities = 125/206 (60%), Positives = 161/206 (78%), Gaps = 4/206 (1%)
Frame = +3
Query: 18 IISLFILNFLYVTSAIRFFVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKASIQ 185
++ LF+L V AI F + P +KCL+EEIHK++LVTG Y+I+D A G ++ ++
Sbjct: 18 LLLLFLLGPRLVL-AISFHL-PINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLK 75
Query: 186 VTDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVETK 365
+TDS H+LY K+D KGKFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE K
Sbjct: 76 ITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVEAK 133
Query: 366 NYDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSLFS 545
NY+ +A+ EKLKPLEVEL+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS+FS
Sbjct: 134 NYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFS 193
Query: 546 MACLMGLAIWQVLYLRRYFKSKKLIE 623
M CL+GLA WQV YLRR+FK+KKLIE
Sbjct: 194 MFCLIGLATWQVFYLRRFFKAKKLIE 219
>sp|Q5RE32|TMEDA_PONPY Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (21 kDa transmembrane
trafficking protein)
Length = 219
Score = 246 bits (628), Expect = 6e-65
Identities = 125/206 (60%), Positives = 160/206 (77%), Gaps = 4/206 (1%)
Frame = +3
Query: 18 IISLFILNFLYVTSAIRFFVTPNGGKKCLKEEIHKNVLVTGEYDITD----APGQKASIQ 185
++ LF+L V AI F + P +KCL+EEIHK++LVTG Y+I+D A G ++ ++
Sbjct: 18 LLLLFLLGPSLVL-AISFHL-PINSRKCLREEIHKDLLVTGAYEISDQSGGAGGLRSHLK 75
Query: 186 VTDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGVETK 365
+TDS H+LY K+D KGKFAFTTE+Y+MF+VC E+K G G+ PD+ V LD+KHGVE K
Sbjct: 76 ITDSAGHILYSKEDATKGKFAFTTEDYDMFEVCFESK--GTGRIPDQLVILDMKHGVEAK 133
Query: 366 NYDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFSLFS 545
NY+ +A+ EKLKPLEVEL+RLEDLS+ IV DF YM REE+MRDTNEST+ RV YFS+FS
Sbjct: 134 NYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMEKREEEMRDTNESTNTRVLYFSIFS 193
Query: 546 MACLMGLAIWQVLYLRRYFKSKKLIE 623
M CL+GLA WQV YLRR+FK+KKLIE
Sbjct: 194 MFCLIGLATWQVFYLRRFFKAKKLIE 219
>sp|Q90515|TMEDA_FUGRU Transmembrane emp24 domain containing protein 10 precursor
(Transmembrane protein Tmp21) (S31III125)
Length = 213
Score = 241 bits (616), Expect = 2e-63
Identities = 123/209 (58%), Positives = 156/209 (74%), Gaps = 10/209 (4%)
Frame = +3
Query: 27 LFILNFLYVTSAIRFFVTPNGGKKCLKEEIHKNVLVTGEYDITD----------APGQKA 176
LF+ + +I FF+ P +KCL+EEIHK+VLVTGEY+I++ G +
Sbjct: 8 LFLPVLIESAFSISFFL-PVNTRKCLREEIHKDVLVTGEYEISEQVVTVHTSSTVVGDGS 66
Query: 177 SIQVTDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETKLMGGGKGPDREVFLDIKHGV 356
++TDS SH LY K+D KGKFAFTTE+Y+MF+VC E+K G+ PD+ V LD+KHGV
Sbjct: 67 IFKITDSSSHTLYSKEDATKGKFAFTTEDYDMFEVCFESKCT--GRVPDQLVNLDMKHGV 124
Query: 357 ETKNYDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTHNRVFYFS 536
E KNY+ +A+ EKLKPLEVEL+RLEDLS+ IV DF YM+ REE+MRDTNEST+ RV YFS
Sbjct: 125 EAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFS 184
Query: 537 LFSMACLMGLAIWQVLYLRRYFKSKKLIE 623
+FSM CL+GLA WQV YLRR+FK+KKLIE
Sbjct: 185 IFSMFCLIGLATWQVFYLRRFFKAKKLIE 213
>sp|P54837|ERV25_YEAST Protein ERV25 precursor
Length = 211
Score = 134 bits (336), Expect = 5e-31
Identities = 70/177 (39%), Positives = 111/177 (62%), Gaps = 3/177 (1%)
Frame = +3
Query: 99 CLKEEIHKNVLVTGEY--DITDAPGQKASIQVTDSKSHVLYQKDDIAKG-KFAFTTEEYE 269
C+++ + + LV + D + GQK ++ V DS + +K D A + AFT
Sbjct: 35 CIRDFVTEGQLVVADIHSDGSVGDGQKLNLFVRDSVGNEYRRKRDFAGDVRVAFTAPSST 94
Query: 270 MFDVCVETKLMGGGKGPDREVFLDIKHGVETKNYDSLAQAEKLKPLEVELKRLEDLSDEI 449
FDVC E + G+ R + LDI+ G E ++++ ++ EKLKP+EVEL+R+E+++DEI
Sbjct: 95 AFDVCFENQAQYRGRSLSRAIELDIESGAEARDWNKISANEKLKPIEVELRRVEEITDEI 154
Query: 450 VKDFQYMRDREEKMRDTNESTHNRVFYFSLFSMACLMGLAIWQVLYLRRYFKSKKLI 620
V + Y+++REE++RDTNEST+ RV FS+ + L L +WQV YL+ YFK+K +I
Sbjct: 155 VDELTYLKNREERLRDTNESTNRRVRNFSILVIIVLSSLGVWQVNYLKNYFKTKHII 211
>sp|Q9BVK6|TMED9_HUMAN Transmembrane emp24 domain containing protein 9 precursor
(Glycoprotein 25L2)
Length = 214
Score = 97.8 bits (242), Expect = 4e-20
Identities = 60/215 (27%), Positives = 109/215 (50%), Gaps = 16/215 (7%)
Frame = +3
Query: 24 SLFILNFLYVTSAIRFFVTPNGGKKCLKEEIHKNVLVTGEY--DITD---------APGQ 170
+L ++ +L + +F KKC EEI +V G Y + D PG
Sbjct: 3 TLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGL 62
Query: 171 KASIQVTDSKSHVLYQKDDIAKGKFAFTTEEYEMFDVCVETK-----LMGGGKGPDREVF 335
++V D + V+ + ++G+F FT+ +C+ + L GG V
Sbjct: 63 GMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGM---LRVH 119
Query: 336 LDIKHGVETKNYDSLAQAEKLKPLEVELKRLEDLSDEIVKDFQYMRDREEKMRDTNESTH 515
LDI+ G +Y +A +KL L++ +++L + ++I K+ Y R REE+ R T+EST+
Sbjct: 120 LDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTN 179
Query: 516 NRVFYFSLFSMACLMGLAIWQVLYLRRYFKSKKLI 620
RV ++S+ L+ + +WQ+ +L+ +F++KKL+
Sbjct: 180 QRVLWWSILQTLILVAIGVWQMRHLKSFFEAKKLV 214
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,310,517
Number of Sequences: 369166
Number of extensions: 1756388
Number of successful extensions: 5679
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5420
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5632
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9126484700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)